Quantitative reevaluation of the function of Karez using remote sensing technology

The water delivery capacity of Karez is gradually declining, and it is possible that such a traditional water supply systems might be completely replaced by modern water conservancy projects. To determine whether these ancient water conservancy projects have a future, we conducted a study in a typical research area of Xinjiang Province, China. Using remote sensing technology, in situ surveying, and the analytic hierarchy process, we evaluated Karez in terms of water delivery capacity, water intake convenience, and capability to maintaining the ecological stability of the surrounding area. The derived results are as follows. (1) During 2005–2020, the length of 14 Karez systems in the study area decreased; however, the calculated water access convenience degree (C) indicated that Karez are more convenient for water intake compared with modern transmission channels, i.e., Ckarez = 0.68 and Cchannel = 0.51. (2) The mean annual runoff of the Karez system was 69.38 × 106 m3, similar to that of the modern water transmission channels in the area (73.49 × 106 m3). (3) Change in ecological sensitivity occurred mostly in regions where Karez systems have disappeared, with increase of 70.69 % and 14.51 % in high and medium sensitivity areas, respectively, and decline of 21.53 % and 9.74 % in low-sensitivity and non-sensitivity areas. Our research shows that Karez have considerable water delivery capacity, and that their existence is beneficial to maintaining the stability of the surrounding ecology. The Karez system can be considered a template for harmonious coexistence between humans and nature

Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

Skeletal myogenesis is a highly ordered process finely regulated by various factors. Long non-coding RNAs play an important regulatory role in myogenesis via multiple mechanisms. In this study, we identified the lncRNA Gm10561, which was upregulated during myogenic differentiation and is highly expressed in skeletal muscle. Knockdown of Gm10561 inhibited the proliferation and differentiation of C2C12 myoblasts in vitro and muscle growth in vivo. Overexpression of Gm10561 promoted the proliferation and differentiation of both C2C12 myoblasts and porcine muscle satellite cells. Notably, lncRNA Gm10561 is localized in the cytoplasm and competitively bound to miR-432, which directly targets MEF2C and E2F3. It was confirmed that lncRNA Gm10561 regulates the proliferation and differentiation of myoblasts by acting as a sponge of miR-432 to modulate MEF2C and E2F3 expression. Thus, the lncRNA-Gm10561-miR-432-MEF2C/E2F3 axis plays an important role in myogenesis