Modern approach to neuropsychological assessment as a predictor of computerized cognitive rehabilitation

Neuropsychological tests are specially designed tasks used to assess cognitive function, known to be related to a particular brain structure or pathway. The modern approach in neuropsychological assessment involves the application of the test in a computer application. This approach has many advantages, simple application of other technology and sensors, to get as informative the data. The purpose of this research was to analyze the possibilities for realization of certain neuropsychological tests in the form of computer applications, with the use of an additional sensor and application of the data obtained in rehabilitation. The Wisconsin Card Sorting Test - WCST, a card sorting test, was used as an example of the test. The survey included 20 respondents from the regular population, ages 17-29, selected by the random sample method, who voluntarily accepted to participate in the research. Respondents solved the test in a computer application with an additional sensor attached to an Emotiv Epoc Electroencephalograph. The results show that it takes less time to solve the test in a computer application and that errors that the examiner may make are excluded. Moreover, the additional sensor provides accurate data on registered features that can be used in diagnostics as well as in rehabilitation. All this information cannot otherwise be obtained by the usual test method

Recent advances in flower color alteration by metabolic manipulation of carotenoid biosynthesis

U hortikulturi je prisutna stalna potreba za ukrasnim biljkama sa novim karakteristikama, gde boja cveta predstavlja jednu od najvažnijih osobina koja određuje njihovu komercijalnu vrednost. Sa razvojem metoda genetičkog inženjeringa otvorena je mogućnost kreiranja biljaka sa željenom bojom cvetova koja se ne može postići klasičnim ukrštanjem ili mutagenezom. Boja cvetova kod biljaka određena je sadržajem tri biljna pigmenta: antocijanina, karotenoida i betalaina. Do sada, najveći napredak postignut je genetičkom modulacijom biosinteze antocijanina. Na ovaj način postignute su nove boje cvetova kod najmanje 50 ukrasnih vrsta, a neki od tih modifikovanih varijeteta su već dugi niz godina u slobodnoj prodaji. Međutim, promena boje cveta manipulacijom biosintetskog puta karotenoida je dokumentovana kod svega nekoliko ukrasnih vrsta i poslednjih godina intenzivirana su istraživanja u tom pravcu. U ovom radu je razmatran potencijal ovog pristupa, sa posebnim osvrtom na rezultate postignute na promeni boje cvetova kod kultivara ljubičice uvodjenjem gena za kapsantin-kapsorubin sintazu.In horticulture, there is a constant need for ornamental plants with new characteristics, where the flower color is one of the most important features that determines their commercial value. With the development of genetic engineering methods, it has been possible to create plants with the desired flower color which cannot be achieved by classical breeding or mutagenesis. The flower color in plants is determined by the content of three plant pigments: anthocyanins, carotenoids and betalains. Up to date, the greatest progress has been made by genetic modulation of anthocyanin biosynthesis. In this way, the new flower colors have been achieved in at least 50 ornamental species, and some of these modified varieties have been on market for many years. However, the alteration of flower color by manipulating the carotenoid biosynthetic pathway has been documented in only a few ornamental species, and the research has been significantly increased last few years. In this paper, the potential of this approach is considered, with special reference to the results achieved on flower color alteration of pansy cultivars by introducing the gene for capsanthin-capsorubin synthase

Determination of coincidence summing correction factors for 22na point source

The coincidence summing effect plays an important role in HPGe spectrometry, especially at low source-detector distances, due to a large solid angle; therefore, the calculation of correction factors is necessary. The aim of the research described in this paper was to compare values of correction factors for a 22Na point source obtained using the GESPECOR software package (Monte-Carlo method) and experimentally obtained values. Measurements were performed using a semiconductor HPGe spectrometer and the point source axially positioned at nine different distances from the detector end-cap. For the purpose of determining correction factors, a system of equations was formed, which, besides nuclear data as the input parameters, uses the experimentally obtained values of the total count in the entire spectrum, as well as the counts in the full energy peaks. The system of equations was solved for each particular case and correction factors were determined. By comparing the results obtained using the experimental and Monte-Carlo method, it was found that the correction factors for the 22Na point source have discrepancies less than 3%. The significance of these discrepancies was also verified from a statistical point of view using a Student's t-test. © 2019 RAD Association. All rights reserved.Conference of 6th International Conference on Radiation and Applications in Various Fields of Research, RAD 2018 ; Conference Date: 18 June 2018 Through 22 June 2018; Conference Code:14955

Efficient genetic transformation of Impatiens hawkerii Bull. (Balsamiaceae) using agrobacterium rhizogenes

Transformation of Impatiens hawkerii Bull. mediated by Agrobacterium rhizogenes strain A4M70GUS was studied. Hairy roots developed 10 days after inoculation were excised from the shoot explants and transferred onto Murashige and Skoog's (MS) basal medium lacking plant growth regulators. More than 20 hairy root clones were established and eight of them were further analyzed. Each clone differed significantly from the others in growth capacity and lateral branching. Clone C2 showed the highest biomass (20.6 g L-1) as well as the highest number of lateral roots (37 ± 2.2). The transgenic nature of the established hairy root clones was confirmed by GUS assay and PCR analysis. In conclusion, hairy roots were developed for the first time in I. hawkerii Bull., and transgenic hairy root clones showed a distinct morphological nature and growth patterns.Proučavana je genetička transformacija Impatiens hawkerii Bull. posredstvom Agrobacterium rhizogenes soja A4M70GUS. Deset dana posle inokulacije formirali su se transgeni korenovi na eksplantatima izdanaka, a zatim gajeni na Murashige and Skoog's (MS) osnovnoj hranljivoj podlozi bez biljnih regulatora rastenja. Uspostavljene su kulture više od 20 klonova, a 8 je dalje analizirano. Klonovi su se međusobno značajno razlikovali u odnosu na kapacitet rastenja i bočnog grananja. Klon C2 je imao najveću biomasu (20.6 g L-1), kao i najveći broj bočnih korenova (37 ± 2.2). Prisustvo stranih gena u klonovima transgenih korenova je potvrđeno GUS eseja i PCR analize. Transgeni korenovi su dobijeni prvi put kod Impatiens hawkerii Bull. i pokazuju značajne razlike u morfologiji i parametrima rastenja.Projekat ministarstva br. TR-2301

Radon exhalation rate of some building materials common in Serbia

It is well-known that radon is the second important human carcinogen for lung cancer, after smoking. The major sources of indoor radon concentrations are soil and building material. Under certain conditions, a dose received from the inhalation of radon and its progenies can be higher than a dose received from the external exposure due to radium concentration in building materials. In this contribution, the results of the radon and thoron exhalation rate measurement from 9 commonly used building materials are reported. Exhalation rate measurements were performed with accumulation chamber method using active device for measurement of radon concentration. © 2019 RAD Association. All rights reserved.Conference of 6th International Conference on Radiation and Applications in Various Fields of Research, RAD 2018 ; Conference Date: 18 June 2018 Through 22 June 2018; Conference Code:14955

Morphological description and molecular detection of Pestalotiopsis sp on hazelnut in Serbia

In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch's postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-alpha, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut

Virus elimination from ornamental plants using in vitro culture techniques

Viruses are responsible for numerous epidemics in different crops in all parts of the world. As a consequence of their presence great economic losses are being incurred. In addition to the development of sensitive techniques for detection, identification and characterization of viruses, substantial attention has also been paid to biotechnological methods for their elimination from plants. In this review article, the following biotechnological in vitro culture techniques for virus elimination from ornamental plants are presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or a combination of these methods. The plant species, as well as the type of virus determine the choice of a most suitable method. The state of the art in investigation of virus elimination from Impatiens sp. in Serbia is summarized.Virusi su odgovorni za brojne epidemije na različitim usevima u svim delovima sveta. Posledica njihovog prisustva su velike ekonomske štete, pa osim razvoju osetljivih tehnika za detekciju, identifikaciju i karakterizaciju virusa, velika pažnja se poklanja i biotehnološkim metodama za njihovu eliminaciju. U ovom preglednom radu predstavljene su tehnike in vitro kulture za eliminaciju virusa iz biljnog materijala: kultura meristema, termoterapija, hemoterapija, krioterapija ili kombinacija ovih metoda. Koja će metoda biti primenjena zavisi od biljne vrste, kao i od vrste virusa. U radu je dat pregled istraživanja na eliminaciji virusa iz Impatiens sp. u Srbiji.Projekat ministarstva br. TR-31019 and III4300

Analysis of accuracy and precision of optical 3D digitisation devices in dental computer-aided-design and computer-aided-manufacturing systems

The implementation of intraoral and extraoral computer-aided-design and computer-aided-manufacturing (CAD/CAM) systems in prosthetic dentistry has simplified the procedure, shortened the period of design and manufacture and improved accuracy and aesthetic properties of dental restorations. Three-dimensional (3D) digitisation has become an adequate replacement for conventional dental impressions. The market offers a variety of diverse optical intraoral and extraoral CAD/CAM systems equipped with digitisation devices that are based on different working principles. The main goal of this research is to determine whether precision and accuracy differ among optical digitisation devices. The research includes five high-end devices: Cerec AC, Cerec InEos, Trios, KaVo Everest and Sinergia Scan. The evaluation methodology of the experiment is based on CAD inspection. The results, obtained from accuracy and precision measurements with tolerance levels of 0.01, 0.25 and 0.05 mm, indicate that there is a difference in accuracy and precision between optical digitisation devices based on different working principles

Određivanje korekcionih faktora za koincidentno sumiranje u slučaju tačkastog izvora Y88

У гама спектрометрији се често сусрећемо са ефектом коинцидентног сумирања, а самим тим постоји потреба за одређивањем корекционих фактора. Циљ истраживања описаног у овом раду је поређење вредности корекционих фактора за тачкасти извор 88Y добијених помоћу софтверског пакета GESPECOR (Монте Карло методом) и експериментално добијених вредности. Мерења су обављена помоћу полупроводничког HPGe спектрометра, при чему је тачкасти извор био позициониран на аксијалној оси детектора на девет различитих растојања од капе детектора. Формиран је систем једначина, који осим нуклеарних података, као улазне параметре користи и експериментално добијене вредности тоталног одброја у целом спектру, као и одброја испод фотопикова. На основу решавања система једначина одређени су корекциони фактори за коинцидентно сумирање. Упоређивањем резултата, утврђено је да се вредности корекционих фактора добијених помоћу ове две методе разликују до 3 %.In gamma ray spectrometry often deals with coincidence summing effect, and therefore calculation of correction factors is necessary. Coincidence summing effect play an important role in HPGe spectrometry at low source-detector distances, due to the large solid angle subtended by the detector at the source. The aim of research described in this paper was to compare values of correction factors for point source 88Y obtained using the software package GESPECOR (Monte-Carlo method) and experimentally obtained values. Measurements were performed using a semiconductor HPGe spectrometer, wherein point source was axially located on detector at nine different distances. The system of equations was formed whereas net areas in full energy peaks as well as in entire spectrum were used as input data. The system was solved and correction factors for point source 88Y were determined. By comparing the obtained values, it was found that correction factors obtained by these two methods have discrepancy less than 3 %.Зборник радова : XXIX симпозијум ДЗЗСЦГ : Сребрно језеро, 27-29. септембар 2017. годин

Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull

Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %)