173 research outputs found
Pinning dependent field driven domain wall dynamics and thermal scaling in an ultrathin Pt/Co/Pt magnetic film
Magnetic field-driven domain wall motion in an ultrathin Pt/Co(0.45nm)/Pt
ferromagnetic film with perpendicular anisotropy is studied over a wide
temperature range. Three different pinning dependent dynamical regimes are
clearly identified: the creep, the thermally assisted flux flow and the
depinning, as well as their corresponding crossovers. The wall elastic energy
and microscopic parameters characterizing the pinning are determined. Both the
extracted thermal rounding exponent at the depinning transition, 0.15,
and the Larkin length crossover exponent, 0.24, fit well with the
numerical predictions.Comment: 5 pages, 4 figure
A complement to the modern crystallographer's toolbox: Caged gadolinium complexes with versatile binding modes
A set of seven caged gadolinium complexes were used as vectors for introducing the chelated Gd3+ ion into protein crystals in order to provide strong anomalous scattering for de novo phasing. The complexes contained multidentate ligand molecules with different functional groups to provide a panel of possible interactions with the protein. An exhaustive crystallographic analysis showed them to be nondisruptive to the diffraction quality of the prepared derivative crystals, and as many as 50% of the derivatives allowed the determination of accurate phases, leading to high-quality experimental electron-density maps. At least two successful derivatives were identified for all tested proteins. Structure refinement showed that the complexes bind to the protein surface or solvent-accessible cavities, involving hydrogen bonds, electrostatic and CH-π interactions, explaining their versatile binding modes. Their high phasing power, complementary binding modes and ease of use make them highly suitable as a heavy-atom screen for high-throughput de novo structure determination, in combination with the SAD method. They can also provide a reliable tool for the development of new methods such as serial femtosecond crystallography. © 2014 International Union of Crystallography.Peer Reviewe
MUC1 ectodomain is a flagellin-targeting decoy receptor and biomarker operative during Pseudomonas aeruginosa lung infection.
Five key attributes can increase marine protected areas performance for small-scale fisheries management
Marine protected areas (MPAs) have largely proven to be effective tools for conserving marine ecosystem, while socio-economic benefits generated by MPAs to fisheries are still under debate. Many MPAs embed a no-take zone, aiming to preserve natural populations and ecosystems, within a buffer zone where potentially sustainable activities are allowed. Small-scale fisheries (SSF) within buffer zones can be highly beneficial by promoting local socio-economies. However, guidelines to successfully manage SSFs within MPAs, ensuring both conservation and fisheries goals, and reaching a win-win scenario, are largely unavailable. From the peer-reviewed literature, grey-literature and interviews, we assembled a unique database of ecological, social and economic attributes of SSF in 25 Mediterranean MPAs. Using random forest with Boruta algorithm we identified a set of attributes determining successful SSFs management within MPAs. We show that fish stocks are healthier, fishermen incomes are higher and the social acceptance of management practices is fostered if five attributes are present (i.e. high MPA enforcement, presence of a management plan, fishermen engagement in MPA management, fishermen representative in the MPA board, and promotion of sustainable fishing). These findings are pivotal to Mediterranean coastal communities so they can achieve conservation goals while allowing for profitable exploitation of fisheries resources
Magnetic properties and field-driven dynamics of chiral domain walls in epitaxial Pt/Co/AuxPt1-x trilayers
Chiral domain walls in ultrathin perpendicularly magnetized layers have a Néel structure stabilized by a Dzyaloshinskii-Moriya interaction (DMI) that is generated at the interface between the ferromagnet and a heavy metal. Different interface materials or properties are required above and below a ferromagnetic film in order to generate the structural inversion asymmetry needed to ensure that the DMI arising at the two interfaces does not cancel. Here we report on the magnetic properties of epitaxial Pt/Co/AuxPt1−x trilayers grown by sputtering onto sapphire substrates with 0.6 nm thick Co. As x rises from 0 to 1, a structural inversion asymmetry is progressively generated. We characterize the epilayer structure with x-ray diffraction and cross-sectional transmission electron microscopy, revealing (111) stacking. The saturation magnetization falls as the proximity magnetization in Pt is reduced, whilst the perpendicular magnetic anisotropy Ku rises. The micromagnetic DMI strength D was determined using the bubble expansion technique and also rises from a negligible value when x=0 to ∼1 mJ/m2 for x=1. The depinning field at which field-driven domain wall motion crosses from the creep to the depinning regime rises from ∼40 to ∼70 mT, attributed to greater spatial fluctuations of the domain wall energy with increasing Au concentration. Meanwhile, the increase in DMI causes the Walker field to rise from ∼10 to ∼280 mT, meaning that only in the x=1 sample is the steady flow regime accessible. The full dependence of domain wall velocity on driving field bears little resemblance to the prediction of a simple one-dimensional model, but can be described very well using micromagnetic simulations with a realistic model of disorder. These reveal a rise in Gilbert damping as x increases
Next Generation Sequencing of Nevus Spilus-Type Congenital Melanocytic Nevus: Exquisite Genotype-Phenotype Correlation in Mosaic RASopathies.
The Science of Marine Protected Areas (3rd edition, Mediterranean)
The main purpose of the booklet is to present the latest scientific information about the effects of MPAs in the Mediterranean in order to inform current management dialogues. This is particularly relevant given the increasing legislative frameworks and political initiatives to implement networks of MPAs in countries across the Mediterranean Sea. Importantly, this Edition does much more than simply tailor the earlier content for the Mediterranean region. The edition update the basic content of the booklet, drawing on the wealth of new published scientific literature, highlighting case studies from the Mediterranean Sea
Structural Analysis of the Essential Resuscitation Promoting Factor YeaZ Suggests a Mechanism of Nucleotide Regulation through Dimer Reorganization
Extent: 8p.Background: The yeaZ gene product forms part of the conserved network YjeE/YeaZ/YgjD essential for the survival of many Gram-negative eubacteria. Among other as yet unidentified roles, YeaZ functions as a resuscitation promoting factor required for survival and resuscitation of cells in a viable but non-culturable (VBNC) state. Methodology/Principal Findings: In order to investigate in detail the structure/function relationship of this family of proteins we have performed X-ray crystallographic studies of Vibrio parahaemolyticus YeaZ. The YeaZ structure showed that it has a classic actin-like nucleotide-binding fold. Comparisons of this crystal structure to that of available homologues from E. coli, T. maritima and S. typhimurium revealed two distinctly different modes of dimer formation. In one form, prevalent in the absence of nucleotide, the putative nucleotide-binding site is incomplete, lacking a binding pocket for a nucleotide base. In the second form, residues from the second subunit complete the nucleotide-binding site. This suggests that the two dimer architectures observed in the crystal structures correspond to a free and a nucleotide-bound form of YeaZ. A multiple sequence alignment of YeaZ proteins from different bacteria allowed us to identify a large conserved hydrophobic patch on the protein surface that becomes exposed upon nucleotide-driven dimer re-arrangement. We hypothesize that the transition between two dimer architectures represents the transition between the ‘on’ and ‘off’ states of YeaZ. The effect of this transition is to alternately expose and bury a docking site for the partner protein YgjD. Conclusions/Significance: This paper provides the first structural insight into the putative mechanism of nucleotide regulation of YeaZ through dimer reorganization. Our analysis suggests that nucleotide binding to YeaZ may act as a regulator or switch that changes YeaZ shape, allowing it to switch partners between YjeE and YgjD.Inci Aydin, Yumiko Saijo-Hamano, Keiichi Namba, Connor Thomas and Anna Roujeinikov
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