Effects of student-led drama on nursing students' attitudes to interprofessional working and nursing advocacy: A pre-test post-test educational intervention study

This is the final version. Available from Elsevier via the DOI in this record. Background: Nursing educators need to equip students to work in interprofessional teams and advocate for patients in increasingly integrated health and social care settings. Drama-based education has been used in nursing to help students understand complex concepts and practices, including communication, empathy, and patient safety. However, few studies have evaluated drama-based education to promote understanding of interprofessional care and advocacy, and none have involved student-led drama where students create dramatic performances to support learning. Objectives: To examine the effects of student-led drama on student nurses’ attitudes to interprofessional working and advocacy. Design: Pre-test post-test educational intervention study. Settings: Public university in Scotland. Participants: 400 undergraduate student nurses enrolled on a 15-week module focussed on health and social care integration and interprofessional working. Methods: Students completed paper questionnaires at the start (n = 274, response rate: 80.1 %) and end (n = 175, 63.9 %) of the module. Outcome measures were the validated Attitudes Towards Healthcare Teams Scale (ATHCTS) and Protective Nursing Advocacy Scale (PNAS). Change in mean ATHCTS and PNAS scores were assessed using paired samples t-tests, with Cohen’s d to estimate effect size. Results: ATHCTS scores significantly increased from 3.87 to 4.19 (p < 0.001, d = 0.52). PNAS scores increased from 3.58 to 3.81 (p < 0.001, d = 0.79), with significant improvements in the ‘acting as an advocate’ (4.18 to 4.51, p < 0.001, d = 0.81) and ‘environmental and educational influences’ subscales (3.79 to 4.13, p < 0.001, d = 0.75). Statements focussed on promoting holistic, dignified care and enabling health professionals to be responsive to emotional and financial needs of patients, showed greatest change. Conclusions: Education based on plays created and performed by student nurses led to significant improvements in student nurses’ attitudes towards interprofessional working and nursing advocacy. Student-led drama should be embedded in nursing curricula to enable students to understand the realities and complexities of health and social care integration and interprofessional workingEconomic and Social Research CouncilEdinburgh Napier Universit

On the discovery of stars, quasars, and galaxies in the Southern Hemisphere with S-PLUS DR2

This paper provides a catalogue of stars, quasars, and galaxies for the Southern Photometric Local Universe Survey Data Release 2 (S-PLUS DR2) in the Stripe 82 region. We show that a 12-band filter system (5 Sloan-like and 7 narrow bands) allows better performance for object classification than the usual analysis based solely on broad bands (regardless of infrared information). Moreover, we show that our classification is robust against missing values. Using spectroscopically confirmed sources retrieved from the Sloan Digital Sky Survey DR16 and DR14Q, we train a random forest classifier with the 12 S-PLUS magnitudes + 4 morphological features. A second random forest classifier is trained with the addition of the W1 (3.4 μm) and W2 (4.6 μm) magnitudes from the Wide-field Infrared Survey Explorer (WISE). Forty-four per cent of our catalogue have WISE counterparts and are provided with classification from both models. We achieve 95.76 per cent (52.47 per cent) of quasar purity, 95.88 per cent (92.24 per cent) of quasar completeness, 99.44 per cent (98.17 per cent) of star purity, 98.22 per cent (78.56 per cent) of star completeness, 98.04 per cent (81.39 per cent) of galaxy purity, and 98.8 per cent (85.37 per cent) of galaxy completeness for the first (second) classifier, for which the metrics were calculated on objects with (without) WISE counterpart. A total of 2926 787 objects that are not in our spectroscopic sample were labelled, obtaining 335 956 quasars, 1347 340 stars, and 1243 391 galaxies. From those, 7.4 per cent, 76.0 per cent, and 58.4 per cent were classified with probabilities above 80 per cent. The catalogue with classification and probabilities for Stripe 82 S-PLUS DR2 is available for download. © 2021 The Author(s).This work has been supported by a PhD fellowship to the lead author from Fundacao de Amparo à Pesquisa do Estado de Sao Paulo (FAPESP), 2019/01312-2. LN also acknowledges the support of Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior -Brasil (CAPES) -Finance Code 001 and FAPESP through process number 2014/10566-4. LN also thanks the staff of the Astronomy department from the University of Florida, where part of this work was done; Marco Antonio dos Santos and Ulisses Manzo Castello for the technical support; Luis Manrique for the technical support, feedback, and discussions about Machine Learning; Christian Massao Tsujiguchi Takagi and Vin ' icius Amaral Haga for the feedback on the accessibility of the figures in this paper; Gustavo Oliveira Schwarz for building the database. CMdO acknowledges funding from FAPESP through grants 2009/542028 and 2019/26492-3 and funding from the Brazilian National Research Council (CNPq), through grant 309209/2019-6. NSTH acknowledges FAPESP (grants 2017/25835-9 and 2015/22308-2). CQ acknowledges support from FAPESP (grants 2015/11442-0 and 2019/06766-1). AM acknowledges FAPESP scholarship grant 2018/25671-9. CEB acknowledges FAPESP, grant 2016/12331-0. FA-F acknowledges funding for this work from FAPESP grant 2018/20977-2. LSJ acknowledges support from Brazilian agencies FAPESP (2019/10923-5) and CNPq (304819/201794). AAC acknowledges support from Fundacao de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ; grant E26/203.186/2016) and CNPq (grants 304971/2016-2 and 401669/2016-5). EVL acknowledges funding for this work from CNPq grant 169181/2017-0 and CAPES grant 88887.470064/2019-00. MLB acknowledges FAPESP, grants 2018/09165-6 and 2019/23388-0. KMD acknowledges support from FAPERJ (grant E-26/203.184/2017), CNPq (grant 312702/2017-5) and the Serrapilheira Institute (grant Serra-1709-17357). AAC acknowledges support from FAPERJ (grant E26/203.186/2016), CNPq (grants 304971/2016-2 and 401669/2016-5), from the Universidad de Alicante under contract UATALENTO18-02, and from the State Agency for Research of the Spanish MCIU through the `Center of Excellence Severo Ochoa' award to the Instituto de Astrofísica de Andalucía (SEV-2017-0709). ARL acknowledges the financial support from CNPq through the PCI (Programa de Capacitacao Institucional) fellowship. The S-PLUS project, including the T80-South robotic telescope and the S-PLUS scientific survey, was founded as a partnership between FAPESP, the Observatorio Nacional (ON), the Federal University of Sergipe (UFS), and the Federal University of Santa Catarina (UFSC), with important financial and practical contributions from other collaborating institutes in Brazil, Chile (Universidad de La Serena), and Spain (Centro de Estudios de Física del Cosmos de Aragón, CEFCA). We further acknowledge financial support from FAPESP, CNPq, CAPES, FAPERJ, and the Brazilian Innovation Agency (FINEP).Peer reviewe

Uncovering Ubiquitin and Ubiquitin-like Signaling Networks

Microscopic imaging and technolog

Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper.

There is an acknowledged need for in vitro fish intestinal model to help understand dietary exposure to chemicals in the aquatic environment. The presence and use of such models is however largely restrictive due to technical difficulties in the culturing of enterocytes in general and the availability of appropriate established cell lines in particular. In this study, the rainbow trout (Oncorhynchus mykiss) intestinal derived cell line (RTgutGC) was used as a surrogate for the "gut sac" method. To facilitate comparison, RTgutGC cells were grown as monolayers (double-seeded) on permeable Transwell supports leading to a two-compartment intestinal model consisting of polarised epithelium. This two-compartment model divides the system into an upper apical (lumen) and a lower basolateral (portal blood) compartment. In our studies, these cells stained weakly for mucosubstances, expressed the tight junction protein ZO-1 in addition to E-cadherin and revealed the presence of polarised epithelium in addition to microvilli protrusions. The cells also revealed a comparable transepithelial electrical resistance (TEER) to the in vivo situation. Importantly, the cell line tolerated apical saline (1:1 ratio) thus mimicking the intact organ to allow assessment of uptake of compounds across the intestine. Following an exposure over 72 h, our study demonstrated that the RTgutGC cell line under sub-lethal concentrations of copper sulphate (Cu) and modified saline solutions demonstrated uptake of the metal with saturation levels comparable to short term ex situ gut sac preparations. Gene expression analysis revealed no significant influence of pH or time on mRNA expression levels of key stress related genes (i.e. CYP3A, GST, mtA, Pgp and SOD) in the Transwell model. However, significant positive correlations were found between all genes investigated suggesting a co-operative relationship amongst the genes studied. When the outlined characteristics of the cell line are combined with the division of compartments, the RTgutGC double seeded model represents a potential animal replacement model for ecotoxicological studies. Overall, this model could be used to study the effects and predict aquatic gastrointestinal permeability of metals and other environmentally relevant contaminants in a cost effective and high throughput manner

Adopting a child perspective for exposome research on mental health and cognitive development - Conceptualisation and opportunities.

Mental disorders among children and adolescents pose a significant global challenge. The exposome framework covering the totality of internal, social and physical exposures over a lifetime provides opportunities to better understand the causes of and processes related to mental health, and cognitive functioning. The paper presents a conceptual framework on exposome, mental health, and cognitive development in children and adolescents, with potential mediating pathways, providing a possibility for interventions along the life course. The paper underscores the significance of adopting a child perspective to the exposome, acknowledging children's specific vulnerability, including differential exposures, susceptibility of effects and capacity to respond; their susceptibility during development and growth, highlighting neurodevelopmental processes from conception to young adulthood that are highly sensitive to external exposures. Further, critical periods when exposures may have significant effects on a child's development and future health are addressed. The paper stresses that children's behaviour, physiology, activity pattern and place for activities make them differently vulnerable to environmental pollutants, and calls for child-specific assessment methods, currently lacking within today's health frameworks. The importance of understanding the interplay between structure and agency is emphasized, where agency is guided by social structures and practices and vice-versa. An intersectional approach that acknowledges the interplay of social and physical exposures as well as a global and rural perspective on exposome is further pointed out. To advance the exposome field, interdisciplinary efforts that involve multiple scientific disciplines are crucial. By adopting a child perspective and incorporating an exposome approach, we can gain a comprehensive understanding of how exposures impact children's mental health and cognitive development leading to better outcomes

Fossil record of stem groups employed in evaluating the chronogram of insects (Arthropoda: Hexapoda)

Insecta s. str. (=Ectognatha), comprise the largest and most diversified group of living organisms, accounting for roughly half of the biodiversity on Earth. Understanding insect relationships and the specific time intervals for their episodes of radiation and extinction are critical to any comprehensive perspective on evolutionary events. Although some deeper nodes have been resolved congruently, the complete evolution of insects has remained obscure due to the lack of direct fossil evidence. Besides, various evolutionary phases of insects and the corresponding driving forces of diversification remain to be recognized. In this study, a comprehensive sample of all insect orders was used to reconstruct their phylogenetic relationships and estimate deep divergences. The phylogenetic relationships of insect orders were congruently recovered by Bayesian inference and maximum likelihood analyses. A complete timescale of divergences based on an uncorrelated log-normal relaxed clock model was established among all lineages of winged insects. The inferred timescale for various nodes are congruent with major historical events including the increase of atmospheric oxygen in the Late Silurian and earliest Devonian, the radiation of vascular plants in the Devonian, and with the available fossil record of the stem groups to various insect lineages in the Devonian and Carboniferous. © The Author(s) 2016