Automated design of bacterial genome sequences

Background: Organisms have evolved ways of regulating transcription to better adapt to varying environments. Could the current functional genomics data and models support the possibility of engineering a genome with completely rearranged gene organization while the cell maintains its behavior under environmental challenges? How would we proceed to design a full nucleotide sequence for such genomes? Results: As a first step towards answering such questions, recent work showed that it is possible to design alternative transcriptomic models showing the same behavior under environmental variations than the wild-type model. A second step would require providing evidence that it is possible to provide a nucleotide sequence for a genome encoding such transcriptional model. We used computational design techniques to design a rewired global transcriptional regulation of Escherichia coli, yet showing a similar transcriptomic response than the wild-type. Afterwards, we “compiled” the transcriptional networks into nucleotide sequences to obtain the final genome sequence. Our computational evolution procedure ensures that we can maintain the genotype-phenotype mapping during the rewiring of the regulatory network. We found that it is theoretically possible to reorganize E. coli genome into 86% fewer regulated operons. Such refactored genomes are constituted by operons that contain sets of genes sharing around the 60% of their biological functions and, if evolved under highly variable environmental conditions, have regulatory networks, which turn out to respond more than 20% faster to multiple external perturbations. Conclusions: This work provides the first algorithm for producing a genome sequence encoding a rewired transcriptional regulation with wild-type behavior under alternative environments

Public Goods and Territory

The paper will attempt to demostrate the fundamental importance of public goods in the performance of any territory or economy. After discussing the concept and definition of public goods, the most important of them will be reviewed along with their degree of publicness. Their relative positions within society will also be analyzed. Finally, the relationship between private and public goods will be discussed and some conclusions will be reached as to the necessary balance and relative importance among them.

Novel and Diverse Recommendations by Leveraging Linear Models with User and Item Embeddings

[Abstract] Nowadays, item recommendation is an increasing concern for many companies. Users tend to be more reactive than proactive for solving information needs. Recommendation accuracy became the most studied aspect of the quality of the suggestions. However, novel and diverse suggestions also contribute to user satisfaction. Unfortunately, it is common to harm those two aspects when optimizing recommendation accuracy. In this paper, we present EER, a linear model for the top-N recommendation task, which takes advantage of user and item embeddings for improving novelty and diversity without harming accuracy.This work was supported by project RTI2018-093336-B-C22 (MCIU & ERDF), project GPC ED431B 2019/03 (Xunta de Galicia & ERDF) and accreditation ED431G 2019/01 (Xunta de Galicia & ERDF). The first author also acknowledges the support of grant FPU17/03210 (MCIU)Xunta de Galicia; ED431B 2019/03Xunta de Galicia; ED431G 2019/0

Is it Worth Refining Linear Approximations to Non-Linear Rational Expectations Models?

We characterize the balanced growth path of the basic neoclassical growth economy using standard, almost linear numerical solution methods, as well as the parameterized expectations approach, which preserves the nonlinearity in the model. We also apply the same methods after adding indivisible labor to the basic model, and to a monetary version of that economy, subject to a cash-in-advance constraint. In a unified framework we tackle the question of how much of the nonlinear structure of the original problem is useful to maintain when using an “almost” linear method. We show that it is possible to find an almost linear method to solve these models as accurately as by parameterizing expectations. Our results show the importance of performing log-linear approximations, as well as the convenience of refining a linear solution method by mixing some structure of the original non-linear problem with structure of the approximated system.Linear-quadratic approximation, numerical accuracy, simulation,numerical methods.

Tools for managing knowledge in SMEs and laggard regions

As the biggest producers and employers of the system, in general SMEs in laggard regions need to keep pace with the expanding rate of technological change that is taking place. To achieve that goal, adequate tools must be developed to generate sufficient transfer of knowledge to those regions and SMEs. The paper will analyze past development and the current situation of Technological Institutes (TI) as a feasible tool for transfering and managing technical change in SMEs. Analyzing their characteristics and proposing how they should be related to the industrial fabric of the region will be the main output of the paper.

Climate Change, Climate Variability and Brucellosis

In addition to natural climate variability observed over comparable time periods, climate change is attributed directly or indirectly to human activity, altering the composition of global atmosphere. This phenomenon continues to be a significant and global threat for the humankind, and its impact compromises many aspects of the society at different levels, including health. The impact of climate change on zoonotic diseases has been largely ignored, particularly brucellosis. We here review some direct and indirect evidences of the impact of climate change and climate variability on brucellosis

Diagnóstico de la calidad del agua implementando parámetros de sólidos suspendidos volátiles y totales para la evaluación del potencial de autodepuración

Trabajo de InvestigaciónLa problemática de la calidad del agua se genera por actividades comunes de las personas y en algunas ocasiones su manejo es inadecuado, por ejemplo; evacuación por medio de la tubería hidrosanitaria o desecho de residuos peligrosos en laderas o canales de desagüe, esto puede provocar impactos negativos en el consumo de la mismaINTRODUCCION 1. GENERALIDADES 2. ANTECEDENTES 3. PLANTEAMIENTO Y FORMULACIÓN DEL PROBLEMA. 4. OBJETIVOS 5. JUSTIFICACIÓN 6. ALCANCES Y LIMITACIONES 8. MARCO DE REFERENCIA 9. ESTADO DEL ARTE 10. METODOLOGÍA 11. ESTRATEGIAS DE COMUNICACIÓN Y DIVULGACIÓN 12. CONCLUSIONES 13. RECOMENDACIONES BIBLIOGRAFIA ANEXOSPregradoIngeniero Civi