PON1 status does not influence cholinesterase activity in Egyptian agricultural workers exposed to chlorpyrifos.

Animal studies have shown that paraoxonase 1 (PON1) genotype can influence susceptibility to the organophosphorus pesticide chlorpyrifos (CPF). However, Monte Carlo analysis suggests that PON1 genotype may not affect CPF-related toxicity at low exposure conditions in humans. The current study sought to determine the influence of PON1 genotype on the activity of blood cholinesterase as well as the effect of CPF exposure on serum PON1 in workers occupationally exposed to CPF. Saliva, blood and urine were collected from agricultural workers (n=120) from Egypt's Menoufia Governorate to determine PON1 genotype, blood cholinesterase activity, serum PON1 activity towards chlorpyrifos-oxon (CPOase) and paraoxon (POase), and urinary levels of the CPF metabolite 3,5,6-trichloro-2-pyridinol (TCPy). The PON1 55 (P≤0.05) but not the PON1 192 genotype had a significant effect on CPOase activity. However, both the PON1 55 (P≤0.05) and PON1 192 (P≤0.001) genotypes had a significant effect on POase activity. Workers had significantly inhibited AChE and BuChE after CPF application; however, neither CPOase activity nor POase activity was associated with ChE depression when adjusted for CPF exposure (as determined by urinary TCPy levels) and stratified by PON1 genotype. CPOase and POase activity were also generally unaffected by CPF exposure although there were alterations in activity within specific genotype groups. Together, these results suggest that workers retained the capacity to detoxify chlorpyrifos-oxon under the exposure conditions experienced by this study population regardless of PON1 genotype and activity and that effects of CPF exposure on PON1 activity are minimal

Shifting the focus: Increasing engagement and improving performance of nursing students in science subjects using face-to-face workshops to reduce anxiety

It is generally accepted that, as a group, undergraduate nursing students struggle with science subjects and this is often attributed to deficiencies in their background knowledge. However, nursing students also report feeling anxious about science subjects and this “science anxiety” could also influence student performance. In an attempt to deal with student’s science anxiety, for the past 2 years we have conducted voluntary, two-day “pre-science workshops” for nursing students. These face-to-face sessions are run 1-2 weeks before the first science subject and are aimed at reducing anxiety, increase self-efficacy, and encouraging mastery-approach goals. When science content is presented it is to stimulate discussions about its relevance to the student’s chosen career, rather than to address deficiencies in their science knowledge. To date, 186 students have attended the workshops. In follow-up interviews students report that the sessions reduced their anxiety and enhanced their engagement with science subjects. In addition, the final grades of attendees, and their progression through the nursing course, were found to be significantly higher than non-attendees. These outcomes demonstrate that programs targeted towards reducing anxiety and increasing engagement with science can improve nursing student’s engagement with and performance in bioscience subjects

Shifting the Focus: Increasing Engagement and Improving Performance of Nursing Students in Bioscience Subjects Using Face-to-Face Workshops to Reduce Anxiety

The difficulty many undergraduate nursing students have with science subjects is often attributed to deficiencies in background knowledge, but anxiety about science subjects could also be involved. While reports indicate that nursing students feel anxious about science-based subjects, the cause of this anxiety and how to reduce it has received less attention. This project aimed 1) to identify the sources of anxiety experienced by nursing students at the start of their first year, and 2) to develop a two-day workshop to reduce anxiety and enhance engagement with science subjects. When surveyed, a substantial proportion of nursing students reported feeling anxious about a range of science and non-science-based activities. To address this anxiety, a two-day “Get Ready for Science” face-to-face workshop was developed and made available to nursing students. Attendees report that the workshops reduced their anxiety and enhanced their engagement with science subjects. In addition, the final grades and the rate of progression through the nursing course of workshop attendees have been significantly higher than non-attendees. These outcomes demonstrate that face-to-face workshops aimed at reducing anxiety can improve nursing student’s engagement with, and performance in, bioscience subjects

Experimental evidence for phonemic contrasts in a nonhuman vocal system

The ability to generate new meaning by rearranging combinations of meaningless sounds is a fundamental component of language. Although animal vocalizations often comprise combinations of meaningless acoustic elements, evidence that rearranging such combinations generates functionally distinct meaning is lacking. Here, we provide evidence for this basic ability in calls of the chestnut-crowned babbler (Pomatostomus ruficeps), a highly cooperative bird of the Australian arid zone. Using acoustic analyses, natural observations, and a series of controlled playback experiments, we demonstrate that this species uses the same acoustic elements (A and B) in different arrangements (AB or BAB) to create two functionally distinct vocalizations. Specifically, the addition or omission of a contextually meaningless acoustic element at a single position generates a phoneme-like contrast that is sufficient to distinguish the meaning between the two calls. Our results indicate that the capacity to rearrange meaningless sounds in order to create new signals occurs outside of humans. We suggest that phonemic contrasts represent a rudimentary form of phoneme structure and a potential early step towards the generative phonemic system of human language

Spiny Mice (\u3cem\u3eAcomys\u3c/em\u3e) Exhibit Attenuated Hallmarks of Aging and Rapid Cell Turnover after UV Exposure in the Skin Epidermis

The study of long-lived and regenerative animal models has revealed diverse protective responses to stressors such as aging and tissue injury. Spiny mice (Acomys) are a unique mammalian model of skin wound regeneration, but their response to other types of physiological skin damage has not been investigated. In this study, we examine how spiny mouse skin responds to acute UVB damage or chronological aging compared to non-regenerative C57Bl/6 mice (M. musculus). We find that, compared to M. musculus, the skin epidermis in A. cahirinus experiences a similar UVB-induced increase in basal cell proliferation but exhibits increased epidermal turnover. Notably, A. cahirinus uniquely form a suprabasal layer co-expressing Keratin 14 and Keratin 10 after UVB exposure concomitant with reduced epidermal inflammatory signaling and reduced markers of DNA damage. In the context of aging, old M. musculus animals exhibit typical hallmarks including epidermal thinning, increased inflammatory signaling and senescence. However, these age-related changes are absent in old A. cahirinus skin. Overall, we find that A. cahirinus have evolved novel responses to skin damage that reveals new aspects of its regenerative phenotype

Modulation of SK Channel Trafficking by Beta Adrenoceptors Enhances Excitatory Synaptic Transmission and Plasticity in the Amygdala

Emotionally arousing events are particularly well remembered. This effect is known to result from the release of stress hormones and activation of beta adrenoceptors in the amygdala. However, the underlying cellular mechanisms are not understood. Small conductance calcium-activated potassium (SK) channels are present at glutamatergic synapses where they limit synaptic transmission and plasticity. Here, we show that beta adrenoceptor activation regulates synaptic SK channels in lateral amygdala pyramidal neurons, through activation of protein kinase A. We show that SK channels are constitutively recycled from the postsynaptic membrane and that activation of beta adrenoceptors removes SK channels from excitatory synapses. This results in enhanced synaptic transmission and plasticity. Our findings demonstrate a novel mechanism by which beta adrenoceptors control synaptic transmission and plasticity, through regulation of SK channel trafficking, and suggest that modulation of synaptic SK channels may contribute to beta adrenoceptor-mediated potentiation of emotional memories