Animal-mediated seed dispersal and the demo-genetic configuration across plant colonization gradients

1.Ecologists have long recognized that seed dispersal mutualisms trigger natural regeneration and range expansion of animal-dispersed trees. Yet we lack empirical studies addressing whether frugivore activity counteracts influences founder effects, which reduce genetic diversity at the colonization front of expanding populations. 2.Here we evaluate the contribution, from both demographic and genetic perspectives, of animal frugivores dispersing seeds across an expansion gradient. We used DNA barcoding for frugivores identification and highly polymorphic genetic markers (SSRs) for maternal analysis of juniper seeds to investigate how (1) stand maturity, (2) microhabitat types, and (3) foraging patterns shape the distribution of the maternal progenies along this gradient. 3.We expect a reduced seed rain density and low numbers of source trees contributing to the seed rain at the colonization front, with limited availability of local fruiting trees. We also anticipated that larger frugivore species would promote maternally rich seed rain due to their capability to mix progenies in their stomachs and move further distances across the landscape. 4.Contrary to our expectations, we found that all identified frugivores produced dense and genetically diverse seed rains across the expansion gradient, even at the colonization front characterized by the scarcity of local fruiting trees.Contrary to our expectations, we found that frugivores generated dense, genetically diverse seed rains across the entire expansion gradient, even at the colonization front with a scarcity of local fruiting trees. 5.Our findings shed light on the fundamental and applied implications of plant-frugivore interactions in shaping highly diverse second-growth forests. These results emphasize the necessity of preserving plant-animal mutualistic interactions to ensure the persistence and expansion of natural tree populations, particularly in formerly fragmented landscapes

Genetic Structure of the Azores Islands: a Study using 15 Autosomal Short Tandem Repeat Loci

The Azores archipelago (Portugal), located in the Atlantic Ocean, 1,500 km from the European mainland, is formed by nine islands of volcanic origin. The relative position of these islands allows the definition of three geographical groups: Eastern, Central and Western. Previous studies of the Azores using Short Tandem Repeats (STRs) have highlighted differences in the frequencies of several loci, when compared to Mainland Portugal or Madeira Island. Furthermore, linkage disequilibrium (LD), described for Azorean samples has been tentatively explained as reflecting the presence of genetic sub-structuring in the archipelago. To provide information concerning the genetic profile of the Azores Islands and to evaluate the presence of substructuring we have determined the allelic frequencies of 15 autosomal STR loci, using the AmpFlSTR® Identifiler™ Kit, in representative samples from the Azorean Islands. Either considering the Azores as a whole, or analysing by island all the loci were in conformity with Hardy-Weinberg equilibrium. Average gene diversity ranged from 0.7669 in Corvo to 0.7972 in Terceira Island. Allelic independence between loci, tested for the global sample, detected significant LD (after correction for multiple tests) for pairs D21S11/D7S820 and D3S1358/D5S818. The exact test of population differentiation, combining the information of the 15 markers analysed, revealed significant differences between the three groups of islands, and between islands. Inter-island analysis reinforces the previous data that suggested the existence of sub-structuring in the Azores archipelago. Moreover, the data generated by this study can be used in a future forensic genetic database of the Azores after the appropriate enlacement of sample size by island, preventing, in that way, misinterpretations caused by population substructuring and small sample sizes

COPD And Family Care: Orientations For Developing Psychoeducational Programs

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COVID-19 Severity and Survival over Time in Patients with Hematologic Malignancies: A Population-Based Registry Study

Mortality rates for COVID-19 have declined over time in the general population, but data in patients with hematologic malignancies are contradictory. We identified independent prognostic factors for COVID-19 severity and survival in unvaccinated patients with hematologic malignancies, compared mortality rates over time and versus non-cancer inpatients, and investigated post COVID-19 condition. Data were analyzed from 1166 consecutive, eligible patients with hematologic malignancies from the population-based HEMATO-MADRID registry, Spain, with COVID-19 prior to vaccination roll-out, stratified into early (February–June 2020; n = 769 (66%)) and later (July 2020–February 2021; n = 397 (34%)) cohorts. Propensity-score matched non-cancer patients were identified from the SEMI-COVID registry. A lower proportion of patients were hospitalized in the later waves (54.2%) compared to the earlier (88.6%), OR 0.15, 95%CI 0.11–0.20. The proportion of hospitalized patients admitted to the ICU was higher in the later cohort (103/215, 47.9%) compared with the early cohort (170/681, 25.0%, 2.77; 2.01–3.82). The reduced 30-day mortality between early and later cohorts of non-cancer inpatients (29.6% vs. 12.6%, OR 0.34; 0.22–0.53) was not paralleled in inpatients with hematologic malignancies (32.3% vs. 34.8%, OR 1.12; 0.81–1.5). Among evaluable patients, 27.3% had post COVID-19 condition. These findings will help inform evidence-based preventive and therapeutic strategies for patients with hematologic malignancies and COVID-19 diagnosis.Depto. de MedicinaFac. de MedicinaTRUEFundación Madrileña de Hematología y HemoterapiaFundación Leucemia y LinfomaAsociación Madrileña de Hematología y Hemoterapiapu

The PI3K/Akt1 pathway enhances steady-state levels of FANCL

Fanconi anemia hematopoietic stem cells display poor self-renewal capacity when subjected to a variety of cellular stress. This phenotype raises the question of whether the Fanconi anemia proteins are stabilized or recruited as part of a stress response and protect against stem cell loss. Here we provide evidence that FANCL, the E3 ubiquitin ligase of the Fanconi anemia pathway, is constitutively targeted for degradation by the proteasome. We confirm biochemically that FANCL is polyubiquitinated with Lys-48-linked chains. Evaluation of a series of N-terminal-deletion mutants showed that FANCL's E2-like fold may direct ubiquitination. In addition, our studies showed that FANCL is stabilized in a complex with axin1 when glycogen synthase kinase-3β is overexpressed. This result leads us to investigate the potential regulation of FANCL by upstream signaling pathways known to regulate glycogen synthase kinase-3β. We report that constitutively active, myristoylated-Akt increases FANCL protein level by reducing polyubiquitination of FANCL. Two-dimensional PAGE analysis shows that acidic forms of FANCL, some of which are phospho-FANCL, are not subject to polyubiquitination. These results indicate that a signal transduction pathway involved in self-renewal and survival of hematopoietic stem cells also functions to stabilize FANCL and suggests that FANCL participates directly in support of stem cell function

Estudios de Caso sobre Ciencias Agropecuarias y Rurales en el siglo XXI.

Libro científico sobre estudios de casos en el medio agropecuario y ruralCon el advenimiento del siglo XXI y el avance de los procesos de globalización, el medio rural presenta diversos cambios económicos, sociales, políticos y culturales. Lo anterior significa que el campo es un objeto de estudio altamente dinámico, complejo e inasible. las ciencias agropecuarias y rurales, en la actualidad, requieren de un abordaje sistémico e interdisciplinario que den cuenta de la heterogeneidad de situaciones y contextos que enfrenta el campo mexicano. La presente obra agrupa 18 estudios de caso, que capturan algunas fotografías de las diversas problemáticas de la ruralidad mexicana, con lo cual se pretende dar cuenta tanto de los objetivos de estudio como de la perspectiva teórico metodológico desde que estos son abordados. lo anterior tiene que ver con el hecho de que las ciencias agropecuarias y rurales manifiestan un alto grado de observación empírica, motivo por el que los estudios de caso se convierten en la perspectiva metodológica idónea que permite ir y venir de la realidad a la teoría y viceversa para la construcción de objetos de estudio. En este volumen se aborda una gran diversidad de casos, que sintetizan la heterogeneidad de enfoques y perspectivas mediante las cuales los fenómenos agropecuarios y rurales han sido abordados en el Instituto de Ciencias Agropecuarias y Rurales de la Universidad Autónoma del Estado de México, en los últimos 30 años

Crear y diseñar un punto de venta de Óptica Maja para incrementar las ventas en Ambato

1. Estudio de mercado 2. Estudio Técnico 3. Introducción del estudio administrativo 4. Estudio Económico.Óptica Maja es una pequeña empresa dedicada a realizar exámenes visuales optométricos y a la venta de armazones, lentes, gafas en diferente calidad; el capital empleado en la inversión del negocio propio; las ventas de Óptica Maja están reducidas.Pontificia Universidad Católica del Ecuador, Escuela de Administración de EmpresasTecnólogo en Administracion de Pequeñas y Medianas Empresa

Prevalence and clinical associations of wheezes and crackles in the general population: The Tromsø study

Background - Wheezes and crackles are well-known signs of lung diseases, but can also be heard in apparently healthy adults. However, their prevalence in a general population has been sparsely described. The objective of this study was to determine the prevalence of wheezes and crackles in a large general adult population and explore associations with self-reported disease, smoking status and lung function. Methods - We recorded lung sounds in 4033 individuals 40 years or older and collected information on self-reported disease. Pulse oximetry and spirometry were carried out. We estimated age-standardized prevalence of wheezes and crackles and associations between wheezes and crackles and variables of interest were analyzed with univariable and multivariable logistic regressions. Results - Twenty-eight percent of individuals had wheezes or crackles. The age-standardized prevalence of wheezes was 18.6% in women and 15.3% in men, and of crackles, 10.8 and 9.4%, respectively. Wheezes were mostly found during expiration and crackles during inspiration. Significant predictors of expiratory wheezes in multivariable analyses were age (10 years increase - OR 1.18, 95%CI 1.09–1.30), female gender (1.45, 1.2–1.8), self-reported asthma (1.36, 1.00–1.83), and current smoking (1.70, 1.28–2.23). The most important predictors of inspiratory crackles were age (1.76, 1.57–1.99), current smoking, (1.94, 1.40–2.69), mMRC ≥2 (1.79, 1.18–2.65), SpO2 (0.88, 0.81–0.96), and FEV1 Z-score (0.86, 0.77–0.95). Conclusions - Nearly over a quarter of adults present adventitious lung sounds on auscultation. Age was the most important predictor of adventitious sounds, particularly crackles. The adventitious sounds were also associated with self-reported disease, current smoking and measures of lung function. The presence of findings in two or more auscultation sites was associated with a higher risk of decreased lung function than solitary findings

Curva de crescimento de diferentes linhagens de frango de corte caipira

Objetivou-se identificar o modelo de regressão não linear mais adequado para descrever a curva de crescimento de quatro linhagens de frangos caipira e compará-las entre as linhagens. Foram utilizadas 100 aves, sendo 13 machos e 12 fêmeas de cada uma das linhagens. Foram testados 13 modelos de regressão não linear e, após a seleção do melhor modelo, foi verificada a igualdade de parâmetros e identidade de modelos não lineares. O peso médio aos 77 dias de idade foi de 3.017, 2.781, 2.724 e 2.587kg para a Pesadão, Mista, Carijó e Pescoço Pelado, respectivamente. O modelo Quadrático Logarítmico apresentou melhor ajuste dos dados para as linhagens Pesadão, Carijó e Mista, segundo o quadrado médio do resíduo e desvio médio absoluto. Para a linhagem Pescoço Pelado, os critérios estatísticos apontaram como mais adequado o modelo Polinomial Inverso, seguido do Quadrático Logarítmico. As curvas de crescimento entre machos e entre fêmeas das linhagens foram diferentes, assim, como as curvas de crescimento de machos e fêmeas dentro de linhagens. A divergência entre as classes avaliadas foi atribuída à taxa de decréscimo de peso, parâmetro c do modelo Quadrático Logarítmico. O modelo Quadrático Logarítmico pode ser utilizado para descrever a curva de crescimento das quatro linhagens. A linhagem Pesadão tem o crescimento mais acelerado e a linhagem Pescoço Pelado tem o crescimento mais lento. A partir do 35o dia de idade, sugere-se o ajuste do manejo nutricional para alterar a curva de crescimento e atender a legislação vigente e o mercado consumidor