Measurement properties of the Dizziness Handicap Inventory by cross-sectional and longitudinal designs

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Effects of DHA- Rich n-3 Fatty Acid Supplementation on Gene Expression in Blood Mononuclear Leukocytes: The OmegAD Study

Background: Dietary fish oil, rich in n-3 fatty acids (n-3 FAs), e. g. docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), regulate inflammatory reactions by various mechanisms, e. g. gene activation. However, the effects of long-term treatment with DHA and EPA in humans, using genome wide techniques, are poorly described. Hence, our aim was to determine the effects of 6 mo of dietary supplementation with an n-3 FA preparation rich in DHA on global gene expression in peripheral blood mononuclear cells. Methods and Findings: In the present study, blood samples were obtained from a subgroup of 16 patients originating from the randomized double-blind, placebo-controlled OmegAD study, where 174 Alzheimer disease (AD) patients received daily either 1.7 g of DHA and 0.6 g EPA or placebo for 6 months. In blood samples obtained from 11 patients receiving n-3 FA and five placebo, expressions of approximately 8000 genes were assessed by gene array. Significant changes were confirmed by real-time PCR. At 6 months, the n-3 FAs group displayed significant rises of DHA and EPA plasma concentrations, as well as up-and down-regulation of nine and ten genes, respectively, was noticed. Many of these genes are involved in inflammation regulation and neurodegeneration, e. g. CD63, MAN2A1, CASP4, LOC399491, NAIP, and SORL1 and in ubiqutination processes, e. g. ANAPC5 and UBE2V1. Down-regulations of ANAPC5 and RHOB correlated to increases of plasma DHA and EPA levels. Conclusions: We suggest that 6 months of dietary n-3 FA supplementatio

Historical biogeography of the leopard (Panthera pardus) and its extinct Eurasian populations

Background: Resolving the historical biogeography of the leopard (Panthera pardus) is a complex issue, because patterns inferred from fossils and from molecular data lack congruence. Fossil evidence supports an African origin, and suggests that leopards were already present in Eurasia during the Early Pleistocene. Analysis of DNA sequences however, suggests a more recent, Middle Pleistocene shared ancestry of Asian and African leopards. These contrasting patterns led researchers to propose a two-stage hypothesis of leopard dispersal out of Africa: an initial Early Pleistocene colonisation of Asia and a subsequent replacement by a second colonisation wave during the Middle Pleistocene. The status of Late Pleistocene European leopards within this scenario is unclear: were these populations remnants of the first dispersal, or do the last surviving European leopards share more recent ancestry with their African counterparts? Results: In this study, we generate and analyse mitogenome sequences from historical samples that span the entire modern leopard distribution, as well as from Late Pleistocene remains. We find a deep bifurcation between African and Eurasian mitochondrial lineages (~ 710 Ka), with the European ancient samples as sister to all Asian lineages (~ 483 Ka). The modern and historical mainland Asian lineages share a relatively recent common ancestor (~ 122 Ka), and we find one Javan sample nested within these. Conclusions: The phylogenetic placement of the ancient European leopard as sister group to Asian leopards suggests that these populations originate from the same out-of-Africa dispersal which founded the Asian lineages. The coalescence time found for the mitochondrial lineages aligns well with the earliest undisputed fossils in Eurasia, and thus encourages a re-evaluation of the identification of the much older putative leopard fossils from the region. The relatively recent ancestry of all mainland Asian leopard lineages suggests that these populations underwent a severe population bottleneck during the Pleistocene. Finally, although only based on a single sample, the unexpected phylogenetic placement of the Javan leopard could be interpreted as evidence for exchange of mitochondrial lineages between Java and mainland Asia, calling for further investigation into the evolutionary history of this subspecies

Injuries among female football players

Background: Football is a popular female team sport played by approximately 40 million women in over 100 countries all over the world. In Sweden football is the largest female team sport with more than 56,000 players over 15 years of age. Aim: The aims of this thesis were to investigate injuries and injury incidences among female non-elite players in second division as well as elite football players in the premiere league in Sweden over an entire football season with special emphases on regional and level differences; to investigate range of motion (ROM) at the beginning of the football season in relationship to upcoming joint (sprain) and muscle-tendon (strain) injuries; to investigate if the injury incidence varied during the different phases of the menstrual cycle and if there was a difference in injury incidence according to oral contraceptive (OC) pill usage. Material and methods: Thirty teams (n=522 players) from two different league levels in Sweden, the second division (9 teams from the most Northern league and 9 teams from the most Southern league, comprising 18 teams) and the premiere league (12 teams), were studied during an entire football season. Baseline information was obtained and ROM was measured. During the season menstruation and OC usage, football exposure and injuries were registered. Result: A total of 466 injuries were studied. The overall injury incidence was 9.6 injuries/1000 hours of football in the second division and 4.6 injuries/1000 hours of football in the premiere league. Traumatic injuries were in majority (59-69%), and the most common type of traumatic injury was sprain, mainly to the ankle. The distribution of injuries varied between regions; the number of total injuries as well as the total injury incidence was higher in the northern than southern region in the second division. Both traumatic and overuse injuries occurred mainly during the early preseason and at the beginning of the competitive spring season. Increased/decreased ROM in the lower extremity did not appear to be a predisposing risk factor for joint (sprain) or muscle-tendon (strain) injuries of the lower extremity. More than half the players began their football season with physical complaints, which in most cases disturbed their football activity. A total of 2 586 menstrual cycles were studied. An increased injuries incidence was noted during the menstrual phase compared to the pre- ovulatory phase as well as during the post-ovulatory phase compared to the pre-ovulatory phase for non-OC users. An increased incidence of traumatic injuries was also noted during the menstrual phase compared to the pre- ovulatory phase for non-OC users. There were no differences between the OC/non-OC groups concerning injury incidence during practice, game or total football. Conclusion: Evidence is presented in this thesis that regional factors as well as play- level are associated with injury incidence. Preseason ROM measurements cannot identify players at risk for upcoming sprain or strain injuries. An increased injury incidence during the menstrual phase was found, however, no significant difference in injury incidence between OC-users and non- users were found.Godkänd; 2006; 20061106 (andbra

Injuries among swedish female elite football players : a prospective population study

Godkänd; 2007; 20071019 (andbra

Injuries among female football players : with special emphasis on regional differences

Godkänd; 2007; 20071019 (andbra

Range of motion in relation to upcoming sprain and strain injuries among female football players

Godkänd; 2007; 20071019 (andbra

Injuries among swedish female elite football players : a prospective population study

Godkänd; 2007; 20071019 (andbra

Concurrent Validity of Cervical Movement Tests Using VR Technology—Taking the Lab to the Clinic

Reduced cervical range of motion (ROM) and movement velocity are often seen in people with neck pain. Objective assessment of movement characteristics is important to identify dysfunction, to inform tailored interventions, and for the evaluation of the treatment effect. The purpose of this study was to investigate the concurrent validity of a newly developed VR technology for the assessment of cervical ROM and movement velocity. VR technology was compared against a gold-standard three-dimensional optical motion capture system. Consequently, 20 people, 13 without and 7 with neck pain, participated in this quantitative cross-sectional study. ROM was assessed according to right/left rotation, flexion, extension, right/left lateral flexion, and four diagonal directions. Velocity was assessed according to fast cervical rotation to the right and left. The correlations between VR and the optical system for cervical ROM and velocity were excellent, with intraclass correlation coefficient (ICC) values &gt; 0.95. The mean biases between VR and the optical system were ≤ 2.1° for the ROM variables, &lt;12°/s for maximum velocity, and ≤3.0°/s for mean velocity. In conclusion, VR is a useful assessment device for ROM and velocity measurements with clinically acceptable biases. It is a feasible tool for the objective measurement of cervical kinematics in the clinic.Validerad;2024;Nivå 2;2024-01-01 (hanlid);Full text license: CC BY</p