Development of a Rapid Fatigue Life Testing Method for Reliability Assessment of Flip-Chip Solder Interconnects

The underlying physics of failure are critical in assessing the long term reliability of power packages in their intended field applications, yet traditional reliability determination methods are largely inadequate when considering thermomechanical failures. With current reliability determination methods, long test durations, high costs, and a conglomerate of concurrent reliability degrading threat factors make effective understanding of device reliability difficult and expensive. In this work, an alternative reliability testing apparatus and associated protocol was developed to address these concerns; targeting rapid testing times with minimal cost while preserving fatigue life prediction accuracy. Two test stands were fabricated to evaluate device reliability at high frequency (60 cycles/minute) with the first being a single-directional unit capable of exerting large forces (up to 20 N) on solder interconnects in one direction. The second test stand was developed to allow for bi-directional application of stress and the integration of an oven to enable testing at elevated steady-state temperatures. Given the high frequency of testing, elevated temperatures are used to emulate the effects of creep on solder fatigue lifetime. Utilizing the mechanical force of springs to apply shear loads to solder interconnects within the devices, the reliability of a given device to withstand repeated cycling was studied using resistance monitoring techniques to detect the number of cycles-to-failure (CTF). Resistance monitoring was performed using specially designed and fabricated, device analogous test vehicles assembled with the ability to monitor circuit resistance in situ. When a resistance rise of 30 % was recorded, the device was said to have failed. A mathematical method for quantifying the plastic work density (amount of damage) sustained by the solder interconnects prior to failure was developed relying on the relationship between Hooke’s Law for springs and damage deflection to accurately assess the mechanical strength of tested devices

A New Approach: Patient Portals for Primary Intervention

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Laser induced photoluminescence spectroscopy of cometary radicals

Flash photolysis together with laser excitation of the product fragments was used in laboratory studies of cometary radicals. The LIPS method has been applied to the CN radical to determine: (1) Radiative lifetimes of individual rotational levels in the zeroth vibrational level of the B state; (2) energy partitioning during photodissociation of C2N2; and (3) vibrational and rotational excitation during formation of CN radicals in the photodissociation of dicyanoacetylene

Beauty and the Flirt: Male Physical Attractiveness and Approaches to Relationship Initiation

This multi-study investigation explored how women evaluate men’s approach strategies. In Study 1, 330 participants generated 546 verbal strategies used in courtship initiation. Strategies were rated on three dimensions that define relationships: affiliation, dominance, and explicitness. Affiliation and explicitness were related to strategy flirtatiousness. In Study 2, 361 females participated in an experiment that explored the effects of four approach strategies used by an attractive or unattractive man on conversation continuance and future interaction. Attractive men using inviting, playful, and less annoying messages were most successful. Study 3 (N = 398) replicated Study 2 using different approach strategies, and also demonstrated male attractiveness, affiliation and explicitness inform message flirtatiousness and influence courtship outcomes. Results suggest approach strategy, but not male attractiveness, influence perceived first date goals

Vector competence of Aedes aegypti, Culex tarsalis, and Culex quinquefasciatus from California for Zika virus.

Zika virus (ZIKV) has emerged since 2013 as a significant global human health threat following outbreaks in the Pacific Islands and rapid spread throughout South and Central America. Severe congenital and neurological sequelae have been linked to ZIKV infections. Assessing the ability of common mosquito species to transmit ZIKV and characterizing variation in mosquito transmission of different ZIKV strains is important for estimating regional outbreak potential and for prioritizing local mosquito control strategies for Aedes and Culex species. In this study, we evaluated the laboratory vector competence of Aedes aegypti, Culex quinquefasciatus, and Culex tarsalis that originated in areas of California where ZIKV cases in travelers since 2015 were frequent. We compared infection, dissemination, and transmission rates by measuring ZIKV RNA levels in cohorts of mosquitoes that ingested blood meals from type I interferon-deficient mice infected with either a Puerto Rican ZIKV strain from 2015 (PR15), a Brazilian ZIKV strain from 2015 (BR15), or an ancestral Asian-lineage Malaysian ZIKV strain from 1966 (MA66). With PR15, Cx. quinquefasciatus was refractory to infection (0%, N = 42) and Cx. tarsalis was infected at 4% (N = 46). No ZIKV RNA was detected in saliva from either Culex species 14 or 21 days post feeding (dpf). In contrast, Ae. aegypti developed infection rates of 85% (PR15; N = 46), 90% (BR15; N = 20), and 81% (MA66; N = 85) 14 or 15 dpf. Although MA66-infected Ae. aegypti showed higher levels of ZIKV RNA in mosquito bodies and legs, transmission rates were not significantly different across virus strains (P = 0.13, Fisher's exact test). To confirm infectivity and measure the transmitted ZIKV dose, we enumerated infectious ZIKV in Ae. aegypti saliva using Vero cell plaque assays. The expectorated plaque forming units PFU varied by viral strain: MA66-infected expectorated 13±4 PFU (mean±SE, N = 13) compared to 29±6 PFU for PR15-infected (N = 13) and 35±8 PFU for BR15-infected (N = 6; ANOVA, df = 2, F = 3.8, P = 0.035). These laboratory vector competence results support an emerging consensus that Cx. tarsalis and Cx. quinquefasciatus are not vectors of ZIKV. These results also indicate that Ae. aegypti from California are efficient laboratory vectors of ancestral and contemporary Asian lineage ZIKV

Tidal Evolution of Close-in Extrasolar Planets: High Stellar Q from New Theoretical Models

In recent years it has been shown that the tidal coupling between extrasolar planets and their stars could be an important mechanism leading to orbital evolution. Both the tides the planet raises on the star and vice versa are important and dissipation efficiencies ranging over four orders of magnitude are being used. In addition, the discovery of extrasolar planets extremely close to their stars has made it clear that the estimates of the tidal quality factor, Q, of the stars based on Jupiter and its satellite system and on main sequence binary star observations are too low, resulting in lifetimes for the closest planets orders of magnitude smaller than their age. We argue that those estimates of the tidal dissipation efficiency are not applicable for stars with spin periods much longer than the extrasolar planets' orbital period. We address the problem by applying our own values for the dissipation efficiency of tides, based on our numerical simulations of externally perturbed volumes of stellar-like convection. The range of dissipation we find for main-sequence stars corresponds to stellar $Q_*$ of $10^8$ to $3{\times}10^9$. The derived orbit lifetimes are comparable to, or much longer than the ages of the observed extrasolar planetary systems. The predicted orbital decay transit timing variations due to the tidal coupling are below the rate of ms/yr for currently known systems, but within reach of an extended Kepler mission provided such objects are found in its field.Comment: Accepted for publication in ApJ 5 pages 3 figure

Recolonizing carnivores: Is cougar predation behaviorally mediated by bears?

Conservation and management efforts have resulted in population increases and range expansions for some apex predators, potentially changing trophic cascades and foraging behavior. Changes in sympatric carnivore and dominant scavenger populations provide opportunities to assess how carnivores affect one another. Cougars (Puma concolor) were the apex predator in the Great Basin of Nevada, USA, for over 80 years. Black bears (Ursus americanus) have recently recolonized the area and are known to heavily scavenge on cougar kills. To evaluate the impacts of sympatric, recolonizing bears on cougar foraging behavior in the Great Basin, we investigated kill sites of 31 cougars between 2009 and 2017 across a range of bear densities. We modeled the variation in feeding bout duration (number of nights spent feeding on a prey item) and the proportion of primary prey, mule deer (Odocoileus hemionus), in cougar diets using mixed-effects models. We found that feeding bout duration was driven primarily by the size of the prey item being consumed, local bear density, and the presence of dependent kittens. The proportion of mule deer in cougar diet across all study areas declined over time, was lower for male cougars, increased with the presence of dependent kittens, and increased with higher bear densities. In sites with feral horses (Equus ferus), a novel large prey, cougar consumption of feral horses increased over time. Our results suggest that higher bear densities over time may reduce cougar feeding bout durations and influence the prey selection trade-off for cougars when alternative, but more dangerous, large prey are available. Shifts in foraging behavior in multicarnivore systems can have cascading effects on prey selection. This study highlights the importance of measuring the impacts of sympatric apex predators and dominant scavengers on a shared resource base, providing a foundation for monitoring dynamic multipredator/scavenger systems

A single H/ACA small nucleolar RNA mediates tumor suppression downstream of oncogenic RAS.

Small nucleolar RNAs (snoRNAs) are a diverse group of non-coding RNAs that direct chemical modifications at specific residues on other RNA molecules, primarily on ribosomal RNA (rRNA). SnoRNAs are altered in several cancers; however, their role in cell homeostasis as well as in cellular transformation remains poorly explored. Here, we show that specific subsets of snoRNAs are differentially regulated during the earliest cellular response to oncogenic RASG12V expression. We describe a novel function for one H/ACA snoRNA, SNORA24, which guides two pseudouridine modifications within the small ribosomal subunit, in RAS-induced senescence in vivo. We find that in mouse models, loss of Snora24 cooperates with RASG12V to promote the development of liver cancer that closely resembles human steatohepatitic hepatocellular carcinoma (HCC). From a clinical perspective, we further show that human HCCs with low SNORA24 expression display increased lipid content and are associated with poor patient survival. We next asked whether ribosomes lacking SNORA24-guided pseudouridine modifications on 18S rRNA have alterations in their biophysical properties. Single-molecule Fluorescence Resonance Energy Transfer (FRET) analyses revealed that these ribosomes exhibit perturbations in aminoacyl-transfer RNA (aa-tRNA) selection and altered pre-translocation ribosome complex dynamics. Furthermore, we find that HCC cells lacking SNORA24-guided pseudouridine modifications have increased translational miscoding and stop codon readthrough frequencies. These findings highlight a role for specific snoRNAs in safeguarding against oncogenic insult and demonstrate a functional link between H/ACA snoRNAs regulated by RAS and the biophysical properties of ribosomes in cancer

Concert recording 2021-03-01

[Track 1]. Halo theme (Halo) / Martin O\u27Donnell and Michael Salvatori ; arranged by Jackson Martin -- [Track 2].To Zanarkand (Final fantasy X) / Nobuo Uematsu ; arranged by John Oeth -- [Track 3]. Legend of Zelda (Zelda BOTW) . Breath of the wild theme ; Ari, los tin the storm / Rajime Wakai and Gareth Cosser ; arranged by Samuel Wilson -- [Track 4]. Donkey country medley (Donkey kong country) / David Wise ; arranged Brett Shock -- [Track 5]. Goldeneye64 DAM (Goldeneye 007) / Grant Kirkhope ; arranged by Greg Aliano -- [Track 6]. Wii sports theme (Wii sports) / Kazumi Totaka ; arranged by Drew Swiecicki -- [Track 7]. Black Thorn City theme (Pokemon heart gold) / Junichi Masuda ; arranged Nathan Yauger -- [Track 8]. Gerudo Valley (Zenda: ocarina of time) ; -- [Track 9]. Ocarina of time (Legend of Zelda) / Koji Kondo ; arranged James Joseph -- [Track 10]. Ophelia\u27s song (Coffee shop tycoon) / Moontan ; arranged Kinley Hyde -- [Track 11]. The ballad of Gay Tony intro theme (Grand theft auto 4: The ballad of Gay Tony) / Johnston, Murphy, and Bortnick -- [Track 12]. Halo medley (Halo) / Martin O\u27Donnell and Michael Salvatori ; arranged Dylan Wenino

Autogenic versus environmental control during development of river biofilm

In the natural environment, microbial community structure of river biofilm is controlled by biotic and abiotic factors. This study explored the capacity to manipulate the structure of microbial communities by modifying environmental conditions during the course of biofilm development. River epilithic biofilm was cultivated in situ on artificial substrates placed parallel to river water flow. Substrates were incubated for 3 and 5.5 weeks in river to allow natural biofilm development, at two sites with contrasting physico-chemical characteristics. The first site (Aurade´ , Gers, France) was located in an agricultural watershed basin and the second site (Larroque, Haute-Garonne, France) was located in a forested watershed basin. After 3 weeks of biofilm development, a subset of substrates was collected from one site and transplanted to the second site where they remained for 2.5 further weeks. Epilithic bacterial community structure (at 3 weeks from each site and at 5.5 weeks from biofilms with and without transplantation) was assessed using PCR-DGGE of 16S rDNA fragment. Biofilm biomass was estimated using ash free dry mass (AFDM). After 3 weeks of development, biofilms from the two sites exhibited comparable AFDM values (average of 1.4¡0.2 g.mx2). A difference between the two sites was observed after 5.5 weeks of development: AFDM decreased for biofilms from the agricultural watershed basin (from 1.4 to 0.18 g.mx2) as a consequence of grazing pressure (Bithynia), and increased for biofilms from the forested agricultural watershed (from 1.4 to 2.6 g.mx2). Microbial community analyses revealed a differentiated community structure between biofilms from the different sites and exhibited a change of microbial community structure after 5.5 weeks of biofilm development. These observations confirm a process of ecological succession in microbial communities. Changing the incubation site during biofilm development modified the trajectory of these ecological successions, suggesting that site characteristics mainly conditioned the structure of these microbial communities