Structure retrieval in liquid-phase electron scattering

Electron scattering on liquid samples has been enabled recently by the development of ultrathin liquid sheet technologies. The data treatment of liquid-phase electron scattering has been mostly reliant on methodologies developed for gas electron diffraction, in which theoretical inputs and empirical fittings are often needed to account for the atomic form factor and remove the inelastic scattering background. In this work, we present an alternative data treatment method that is able to retrieve the radial distribution of all the charged particle pairs without the need of either theoretical inputs or empirical fittings. The merits of this new method are illustrated through the retrieval of real-space molecular structure from experimental electron scattering patterns of liquid water, carbon tetrachloride, chloroform, and dichloromethane. Shown here is the arXiv version

Furin is a chemokine-modifying enzyme: in vitro and in vivo processing of CXCL10 generates a C-terminally truncated chemokine retaining full activity

Chemokines comprise a class of structurally related proteins that are involved in many aspects of leukocyte migration under basal and inflammatory conditions. In addition to the large number of genes, limited processing of these proteins by a variety of enzymes enhances the complexity of the total spectrum of chemokine variants. We have recently shown that the native chemokine CXCL10 is processed at the C terminus, thereby shedding the last four amino acids. The present study was performed to elucidate the mechanism in vivo and in vitro and to study the biological activity of this novel isoform of CXCL10. Using a combination of protein purification and mass spectrometric techniques, we show that the production of C-terminally truncated CXCL10 by primary keratinocytes is inhibited in vivo by a specific inhibitor of pro-protein convertases (e.g. furin) but not by inhibition of matrix metalloproteinases. Moreover, CXCL10 is processed by furin in vitro, which is abrogated by a mutation in the furin recognition site. Using GTRγS binding, C

Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection ar

Wavelength independent SOI polarization splitter based on zero-order arrayed waveguide gratings

Novel Zero-order arrayed waveguide grating (AWG) based polarization splitters in the silicon-on-insulator platform are reported and experimentally demonstrated. These passive devices employ the cladding-induced stress for achieving the polarization splitting function

Subglacial lakes and their changing role in a warming climate

Subglacial lakes are repositories of ancient climate conditions, provide habitats for life and modulate ice flow, basal hydrology, biogeochemical fluxes and geomorphic activity. In this Review, we construct the first global inventory of subglacial lakes (773 in total), which includes 675 from Antarctica (59 newly identified), 64 from Greenland, 2 beneath the Devon Ice Cap, 6 beneath Iceland’s ice caps and 26 from valley glaciers. This inventory is used to evaluate subglacial lake environments, dynamics and their wider impact on ice flow and sediment transport. The behaviour of these lakes is conditioned by their subglacial setting and the hydrological, dynamic and mass balance regime of the overlying ice mass. Regions where climate warming causes ice surface steepening are predicted to have fewer and smaller lakes, but increased activity with higher discharge drainages of shorter duration. Coupling to surface melt and rainfall inputs will modulate fill–drain cycles and seasonally enhance oxic processes. Higher discharges cause large, transient ice flow accelerations but might result in overall net slowdown owing to the development of efficient subglacial drainage. Subglacial lake research requires new drilling technologies and the integration of geophysics, satellite monitoring and numerical modelling to provide insight into the wider role of subglacial lakes in the changing Earth system