Estudio immunohistoquímico de tumores de folículos pilosos caninos

El objetivo de este trabajo ha sido analizar los patrones de expresión de varias citoqueratinas e involucrina en tumores del folículo piloso canino con especial interés en los que exhiben menor diferenciación tricofítica. En el estudio se han utilizado 71 tumores foliculares diagnosticados en el Servicio de Diagnóstico de Histología y Anatomía Patológica Comparadas de la UCO. Las muestras, fijadas en formol al 10%, se procesaron de la forma habitual para diagnóstico histopatológico y se utilizó la técnica ABC para el estudio inmunohistoquímico. Los tumores foliculares se clasificaron histopatológicamente según Golsmidht y cols, (1998) como tricoepiteliomas (22), epiteliomas intracutáneos cornificantes (23) y tricoblastomas (5)y pilomatricomas (21)

Plural valuation of nature for equity and sustainability: Insights from the Global South

Plural valuation is about eliciting the diverse values of nature articulated by different stakeholders in order to inform decision making and thus achieve more equitable and sustainable outcomes. We explore what approaches align with plural valuation on the ground, as well as how different social-ecological contexts play a role in translating plural valuation into decisions and outcomes. Based on a co-constructed analytical approach relying on empirical information from ten cases from the Global South, we find that plural valuation contributes to equitable and sustainable outcomes if the valuation process: 1) is based on participatory value elicitation approaches; 2) is framed with a clear action-oriented purpose; 3) provides space for marginalized stakeholders to articulate their values in ways that can be included in decisions; 4) is used as a tool to identify and help reconcile different cognitive models about human-nature relations; and 5) fosters open communication and collaboration among stakeholders. We also find that power asymmetries can hinder plural valuation. As interest and support for undertaking plural valuation grows, a deeper understanding is needed regarding how it can be adapted to different purposes, approaches, and social-ecological contexts in order to contribute to social equity and sustainability

Ulmus laevis in the Iberian Peninsula: a review of its ecology and conservation

European white elm (Ulmus laevis Pallas) populations are scarce, small and fragmented in the Iberian Peninsula. Due to these characteristics the indigenous status of the species in the region has been questioned, whilst the species? role in Iberian riparian forest ecology has been neglected. Herein we review past studies regarding this species? distribution and ecology in the Iberian Peninsula, with special emphasis on the establishment of conservation priorities. We first present a collection of palaeogeographic, historic and genetic data suggesting that the Iberian Peninsula was a glacial refuge for U. laevis. Secondly, we analyse U. laevis distribution in relation to soil physico- chemical properties and water availability in Spain. Following this, we focus on the reproductive biology of the species, and investigate the effect of masting and empty seed production on predation and regeneration establishment. Finally, based on this knowledge, we propose conservation policies for U. laevis in the Iberian Peninsula

Characterisation of the Putative Effector Interaction Site of the Regulatory HbpR Protein from Pseudomonas azelaica by Site-Directed Mutagenesis

Bacterial transcription activators of the XylR/DmpR subfamily exert their expression control via σ54-dependent RNA polymerase upon stimulation by a chemical effector, typically an aromatic compound. Where the chemical effector interacts with the transcription regulator protein to achieve activation is still largely unknown. Here we focus on the HbpR protein from Pseudomonas azelaica, which is a member of the XylR/DmpR subfamily and responds to biaromatic effectors such as 2-hydroxybiphenyl. We use protein structure modeling to predict folding of the effector recognition domain of HbpR and molecular docking to identify the region where 2-hydroxybiphenyl may interact with HbpR. A large number of site-directed HbpR mutants of residues in- and outside the predicted interaction area was created and their potential to induce reporter gene expression in Escherichia coli from the cognate PC promoter upon activation with 2-hydroxybiphenyl was studied. Mutant proteins were purified to study their conformation. Critical residues for effector stimulation indeed grouped near the predicted area, some of which are conserved among XylR/DmpR subfamily members in spite of displaying different effector specificities. This suggests that they are important for the process of effector activation, but not necessarily for effector specificity recognition

Nutrición parenteral domiciliaria en España 2018. Informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA

Objetivo: comunicar los datos de nutrición parenteral domiciliaria (NPD) obtenidos del registro del grupo NADYA-SENPE (www.nadya-senpe.com) del año 2018. Material y métodos: análisis descriptivo de los datos recogidos de pacientes adultos y pediátricos con NPD en el registro NADYA-SENPE del 1 de enero al 31 de diciembre de 2018. Resultados: se registraron 278 pacientes (54, 7% mujeres), 23 niños y 255 adultos, procedentes de 45 hospitales españoles, lo que representa una tasa de prevalencia de 5, 95 pacientes/millón de habitantes/año 2018. El diagnóstico más frecuente en adultos fue “oncológico paliativo” (22, 0%), seguido de “otros”. En niños fue la enfermedad de Hirschsprung junto con la enterocolitis necrotizante, con cuatro casos (17, 4%). El primer motivo de indicación fue síndrome de intestino corto tanto en niños (60, 9%) como en adultos (35, 7%). El tipo de catéter más utilizado fue el tunelizado tanto en niños (81, 0%) como en adultos (41, 1%). Finalizaron 75 episodios, la causa más frecuente fue el fallecimiento (52, 0%) y el paso a vía oral (33, 3%). Conclusiones: el número de centros y profesionales colaboradores en el registro de pacientes que reciben NPD se mantiene estable, así como las principales indicaciones y los motivos de finalización de la NPD. Aim: To communicate home parenteral nutrition (HPN) data obtained from the HPN registry of the NADYA-SENPE group (www.nadya-senpe.  com) for the year 2018. Material and methods: Descriptive analysis of the data collected from adult and pediatric patients with HPN in the NADYA-SENPE group registry from January 1st, 2018 to December 31st, 2018.  Results: There were 278 patients from 45 Spanish hospitals (54.7% women), 23 children and 255 adults, which represent a prevalence rate of 5.95 patients/million inhabitants/year 2018. The most frequent diagnosis in adults was “palliative cancer” (22.0%), followed by “others”. In children it was Hirschsprung’s disease together with necrotizing enterocolitis, with four cases (17.4%). The first indication was short bowel syndrome in both children (60.9%) and adults (35.7%). The most frequently used type of catheter was tunneled in both children (81.0%) and adults (41.1%). Ending 75 episodes, the most frequent cause was death (52.0%) and change to oral feeding (33.3%). Conclusions: The number of centers and collaborating professionals in the registry of patients receiving HPN remains stable, as well as the main indications and reasons for termination of HPN

Seguimiento de las guías españolas para el manejo del asma por el médico de atención primaria: un estudio observacional ambispectivo

Objetivo Evaluar el grado de seguimiento de las recomendaciones de las versiones de la Guía española para el manejo del asma (GEMA 2009 y 2015) y su repercusión en el control de la enfermedad. Material y métodos Estudio observacional y ambispectivo realizado entre septiembre del 2015 y abril del 2016, en el que participaron 314 médicos de atención primaria y 2.864 pacientes. Resultados Utilizando datos retrospectivos, 81 de los 314 médicos (25, 8% [IC del 95%, 21, 3 a 30, 9]) comunicaron seguir las recomendaciones de la GEMA 2009. Al inicio del estudio, 88 de los 314 médicos (28, 0% [IC del 95%, 23, 4 a 33, 2]) seguían las recomendaciones de la GEMA 2015. El tener un asma mal controlada (OR 0, 19, IC del 95%, 0, 13 a 0, 28) y presentar un asma persistente grave al inicio del estudio (OR 0, 20, IC del 95%, 0, 12 a 0, 34) se asociaron negativamente con tener un asma bien controlada al final del seguimiento. Por el contrario, el seguimiento de las recomendaciones de la GEMA 2015 se asoció de manera positiva con una mayor posibilidad de que el paciente tuviera un asma bien controlada al final del periodo de seguimiento (OR 1, 70, IC del 95%, 1, 40 a 2, 06). Conclusiones El escaso seguimiento de las guías clínicas para el manejo del asma constituye un problema común entre los médicos de atención primaria. Un seguimiento de estas guías se asocia con un control mejor del asma. Existe la necesidad de actuaciones que puedan mejorar el seguimiento por parte de los médicos de atención primaria de las guías para el manejo del asma. Objective: To assess the degree of compliance with the recommendations of the 2009 and 2015 versions of the Spanish guidelines for managing asthma (Guía Española para el Manejo del Asma [GEMA]) and the effect of this compliance on controlling the disease. Material and methods: We conducted an observational ambispective study between September 2015 and April 2016 in which 314 primary care physicians and 2864 patients participated. Results: Using retrospective data, we found that 81 of the 314 physicians (25.8%; 95% CI 21.3–30.9) stated that they complied with the GEMA2009 recommendations. At the start of the study, 88 of the 314 physicians (28.0%; 95% CI 23.4–33.2) complied with the GEMA2015 recommendations. Poorly controlled asthma (OR, 0.19; 95% CI 0.13–0.28) and persistent severe asthma at the start of the study (OR, 0.20; 95% CI 0.12–0.34) were negatively associated with having well-controlled asthma by the end of the follow-up. In contrast, compliance with the GEMA2015 recommendations was positively associated with a greater likelihood that the patient would have well-controlled asthma by the end of the follow-up (OR, 1.70; 95% CI 1.40–2.06). Conclusions: Low compliance with the clinical guidelines for managing asthma is a common problem among primary care physicians. Compliance with these guidelines is associated with better asthma control. Actions need to be taken to improve primary care physician compliance with the asthma management guidelines

Fracturing and cementation of shallow buried Miocene proximal alluvial fan deposits

Fluid flow in fractures and host rocks has been investigated in shallow buried Miocene alluvial fan deposits. A structural, petrographical (optical, CL, SE microscopes and XRD) and geochemical (microprobe and δ18O-δ13C stable isotopes) study has been performed in normal faults affecting Serravalian-Tortonian siliciclastic rocks of the Vallès-Penedès basin. These faults formed during the development of the Vallès-Penedès fault-related syncline, which caused the rotation of the earliest fractures. Faulting occurred continuously before, during and after host rock cementation. Rocks affected by faulting are represented by clay-rich gouges, which formed thanks to the high phyllite clast content within the otherwise clean and mature sandstones and conglomerates. Despite the low permeability of these rocks, cross-fault and fault-parallel fluid flows occurred in most of the faults. Host rocks and veins were cemented by two generations of calcite, i.e. Cc1 and Cc2. Cc1 precipitated from meteoric waters at shallow burial conditions whereas Cc2 precipitated from meteoric waters in a confined aquifer. Palygorskite has been identified in shear zones within the gouges indicating their later formation by interaction of Mg-rich fluids with previous smectites. These fluids probably derived from Miocene seawater expelled from the underlying Transitional–Marine Complex “TMC” by compaction. Sedimentation, fracturing and cementation occurred in a very short lapse time of about 6–7 Ma, between the Serravalian-Tortonian age of the sediments and the end of the extensional tectonics in the Vallès-Penedès fault (Pliocene).This research was performed within the framework of DGICYT Spanish Project CGL2010-18260, Grup Consolidat de Recerca “Geologia Sedimentària” (2009SGR-1451) and the grant 2011FI_B2 00179 supported by the Comissionat per a Universitats i Recerca del Departament d'Innovació, Universitats i Empresa de la Generalitat de Catalunya i el Fons Social Europeu.Peer reviewe

Curcumin-Piperlongumine Hybrids with a Multitarget Profile Elicit Neuroprotection in In Vitro Models of Oxidative Stress and Hyperphosphorylation

Curcumin shows a broad spectrum of activities of relevance in the treatment of Alzheimer’s disease (AD); however, it is poorly absorbed and is also chemically and metabolically unstable, leading to a very low oral bioavailability. A small library of hybrid compounds designed as curcumin analogues and incorporating the key structural fragment of piperlongumine, a natural neuroinflammation inhibitor, were synthesized by a two-step route that combines a three-component reaction between primary amines, β-ketoesters and α-haloesters and a base-promoted acylation with cinnamoyl chlorides. These compounds were predicted to have good oral absorption and CNS permeation, had good scavenging properties in the in vitro DPPH experiment and in a cellular assay based on the oxidation of dichlorofluorescin to a fluorescent species. The compounds showed low toxicity in two cellular models, were potent inductors of the Nrf2-ARE phase II antioxidant response, inhibited PHF6 peptide aggregation, closely related to Tau protein aggregation and were active against the LPS-induced inflammatory response. They also afforded neuroprotection against an oxidative insult induced by inhibition of the mitochondrial respiratory chain with the rotenone-oligomycin A combination and against Tau hyperphosphorylation induced by the phosphatase inhibitor okadaic acid. This multitarget pharmacological profile is highly promising in the development of treatments for AD and provides a good hit structure for future optimization efforts

Análisis de la definición de la matrona, acceso a la formación y programa formativo de este profesional de la salud a nivel internacional, europeo y español

Resumen: La matrona es el profesional sanitario de referencia en relación con el cuidado de la mujer, y su acceso y formación viene regulada a nivel internacional, europeo y español.Debido a que en la actualidad una matrona formada en otros países puede ejercer en España su profesión surge la necesidad de analizar la definición de este profesional en los diferentes contextos, así como las normativas de acceso a la formación y la estructura del programa formativo de la matrona.Para lo cual se llevó a cabo un análisis documental y se analizó la coherencia entre los documentos normativos vigentes en relación con la definición de la matrona, acceso y programa formativo a nivel internacional, europeo y nacional.En este estudio se detectan ligeras diferencias en la definición de este profesional, así como se identifican dos modelos de acceso a la formación que condicionan un itinerario formativo más o menos extenso. Se contempla una modalidad de formación específica independiente y otra modalidad como especialidad enfermera tras la formación como enfermero generalista. Así mismo, se detecta que la matrona tiene reconocidas más competencias de las que actualmente ejerce en el sistema nacional de salud español. Abstract: A midwife is the health professional of reference for the healthcare of women, and their entry to the midwifery profession has been regulated on an International, European, and Spanish basis.As internationally educated midwives can currently practice in Spain, there appears to be a need for a comprehensive assessment of the different International and European definitions of the profession, as well as the different education and training programs.A documental study of the different regulations has been performed in an International, European and National context. An analysis has also been performed on their coherence in respect to the midwifery profession definition, education programs, and profession entry.The differences detected in the results of the study as regards the definition of a midwife, as well as the education programs to enter the profession are discussed. Two main different approaches to midwife education were also found, the independent midwife professional, and the midwife specialist nurse. Midwives were also found to have more competences than those trained in the Spanish Health System. Palabras clave: Competencia clínica, Enfermera obstétrica, Matronas, Keywords: Clinical competence, Nurse midwives, Midwifer