Crustal influx, indentation, ductile thinning and gravity redistribution in a continental wedge: Building a Moldanubian mantled gneiss dome with underthrust Saxothuringian material (European Variscan belt)

27 p.International audience[1] The contribution of lateral forces, vertical load, gravity redistribution and erosion to the origin of mantled gneiss domes in internal zones of orogens remains debated. In the Orlica-Snieznik dome (Moldanubian zone, European Variscan belt), the polyphase tectono-metamorphic history is initially characterized by the development of subhorizontal fabrics associated with medium- to high-grade metamorphic conditions in different levels of the crust. It reflects the eastward influx of a Saxothuringian-type passive margin sequence below a Teplá-Barrandian upper plate. The ongoing influx of continental crust creates a thick felsic orogenic root with HP rocks and migmatitic orthogneiss. The orogenic wedge is subsequently indented by the eastern Brunia microcontinent producing a multiscale folding of the orogenic infrastructure. The resulting kilometre-scale folding is associated with the variable burial of the middle crust in synforms and the exhumation of the lower crust in antiforms. These localized vertical exchanges of material and heat are coeval with a larger crustal-scale folding of the whole infrastructure generating a general uplift of the dome. It is exemplified by increasing metamorphic conditions and younging of 40Ar/39Ar cooling ages toward the extruded migmatitic subdomes cored by HP rocks. The vertical growth of the dome induces exhumation by pure shear-dominated ductile thinning laterally evolving to non-coaxial detachment faulting, while erosion feeds the surrounding sedimentary basins. Modeling of the Bouguer anomaly grid is compatible with crustal-scale mass transfers between a dense superstructure and a lighter infrastructure. The model implies that the Moldanubian Orlica-Snieznik mantled gneiss dome derives from polyphase recycling of Saxothuringian material

Linking habitat quality with genetic diversity: a lesson from great bustards in Spain

P. 411-419The effects of habitat loss and fragmentation on the genetic structure and variability of wild populations have received wide empirical support and theoretical formalization. By contrast, the effects of habitat quality seem largely underinvestigated, partly due to technical difficulties in properly assessing habitat quality. In this study, we combine geographic information system (GIS)-based habitat-quality modelling with a landscape genetics approach based on mitochondrial DNA markers to evaluate the possible influence of habitat quality on the levels and distribution of genetic diversity in a range of natural populations (n = 15) of Otis tarda throughout Spain. Ninety-three percent of the population represented by our countrywide sample lives in good-quality habitats, while 4.5% and 2.5% occur respectively in intermediate and poor habitats. Habitat quality was highly correlated with patch size, population size and population density, indicating the reliability and predictive power of the habitat suitability model. Genetic diversity was significantly correlated with habitat quality, size and density of the population, but not with patch size. Three of a total of 20 existing matrilineages from the species’ current genetic pool are restricted to poor-quality habitats. This study therefore highlights the importance of considering both population genetics and habitat quality in a species of high conservation priority.S

Precision QTL mapping of downy mildew resistance in hop (Humulus lupulus L.)

Hop downy mildew (DM) is an obligate parasite causing severe losses in hop if not controlled. Resistance to this pathogen is a primary goal for hop breeding programs. The objective of this study was to identify QTLs linked to DM resistance. Next-generation-sequencing was performed on a mapping population segregating for DM resistance levels. Cloned plants were grown in a RCBD with three replicates under three environments: greenhouse (GH), field plots in Oregon (OR), Corvallis field plots in Washington (WA), Yakima). The linkage map of 3,341 SNP markers was determined with a four-stage process using Rqtl, TMAP, Joinmap v 4.0 and MERGEMAP. QTL analysis was performed using JMP Genomics and TASSEL 5.0. SNP markers were distributed across 11 linkage groups (LGs) with an average distance between markers of 0.2 cM and total distance of 745.9 cM. QTLs for all three environments were identified using multiple interval mapping. Overall heritability across the three environments varied from h² = 0.38 (GH) to 0.57 (OR). A total of 22 QTLs across 8 LG were identified for DM resistance: 5 identified from OR field data, 12 using WA data and five from GH DM data. No epistasis was observed. This study points out the complexity of genetic control of DM resistance in hop and identifies several markers that can be potentially be used to select for DM resistance in hop. It also provides the first linkage map suitable for genome sequencing due to the high density of SNP markers.Keywords: Pseudoperonospora, Humulus, Genetic map, Downy mildew, QTL mapping, Hop, SNP

3D Molecular Cytology of Hop (Humulus lupulus) Meiotic Chromosomes Reveals Non-disomic Pairing and Segregation, Aneuploidy, and Genomic Structural Variation

Hop (Humulus lupulus L.) is an important crop worldwide, known as the main flavoring ingredient in beer. The diversifying brewing industry demands variation in flavors, superior process properties, and sustainable agronomics, which are the focus of advanced molecular breeding efforts in hops. Hop breeders have been limited in their ability to create strains with desirable traits, however, because of the unusual and unpredictable inheritance patterns and associated non-Mendelian genetic marker segregation. Cytogenetic analysis of meiotic chromosome behavior has also revealed conspicuous and prevalent occurrences of multiple, atypical, non-disomic chromosome complexes, including those involving autosomes in late prophase. To explore the role of meiosis in segregation distortion, we undertook 3D cytogenetic analysis of hop pollen mother cells stained with DAPI and FISH. We used telomere FISH to demonstrate that hop exhibits a normal telomere clustering bouquet. We also identified and characterized a new sub-terminal 180 bp satellite DNA tandem repeat family called HSR0, located proximal to telomeres. Highly variable 5S rDNA FISH patterns within and between plants, together with the detection of anaphase chromosome bridges, reflect extensive departures from normal disomic signal composition and distribution. Subsequent FACS analysis revealed variable DNA content in a cultivated pedigree. Together, these findings implicate multiple phenomena, including aneuploidy, segmental aneuploidy, or chromosome rearrangements, as contributing factors to segregation distortion in hop

Identification of tandem repeat families from long-read sequences of Humulus lupulus

Hop (Humulus lupulus L.) is known for its use as a bittering agent in beer and has a rich history of cultivation, beginning in Europe and now spanning the globe. There are five wild varieties worldwide, which may have been introgressed with cultivated varieties. As a dioecious species, its obligate outcrossing, non-Mendelian inheritance, and genomic structural variability have confounded directed breeding efforts. Consequently, understanding the hop genome represents a considerable challenge, requiring additional resources. In order to facilitate investigations into the transmission genetics of hop, we report here a tandem repeat discovery pipeline developed using k-mer filtering and dot plot analysis of PacBio long-read sequences from the hop cultivar Apollo. From this we identified 17 new and distinct tandem repeat sequence families, which represent candidates for FISH probe development. For two of these candidates, HuluTR120 and HuluTR225, we produced oligonucleotide FISH probes from conserved regions of and demonstrated their utility by staining meiotic chromosomes from wild hop, var. neomexicanus to address, for example, questions about hop transmission genetics. Collectively, these tandem repeat sequence families represent new resources suitable for development of additional cytogenomic tools for hop research

Palaeozoic Basement of the Pyrenees

International audienceIn the Pyrenees, the Cambrian-Lower Ordovician strata represent a quiescent time span with no remarkable tectonic activity, followed by a late Early-Mid Ordovician episode of uplift and erosion that led to the formation of the Sardic unconformity. Silurian sedimentation was widespread and transgressive followed by a Devonian succession characterized by a complex mosaic of sedi-mentary facies. Carboniferous pre-Variscan sediments (Tournaisian-Viséan cherts and limestones) precede the arrival of the synorogenic siliciclastic supplies of the Culm flysch at the Late Serpukhovian. All this succession was subsequently affected by the Serpukhovian-Bashkirian (Variscan) collision, as a result of which, the Palaeozoic rocks were incorporated into the northeastern branch of the Ibero-Armorican Arc

The evolution of acoustic size exaggeration in terrestrial mammals

Recent studies have revealed that some mammals possess adaptations that enable them to produce vocal signals with much lower fundamental frequency (F0) and formant frequency spacing (ΔF) than expected for their size. Although these adaptations are assumed to reflect selection pressures for males to lower frequency components and exaggerate body size in reproductive contexts, this hypothesis has not been tested across a broad range of species. Here we show that male terrestrial mammals produce vocal signals with lower ΔF (but not F0) than expected for their size in mating systems with greater sexual size dimorphism. We also reveal that males produce calls with higher than expected F0 and ΔF in species with increased sperm competition. This investigation confirms that sexual selection favours the use of ΔF as an acoustic size exaggerator, and supports the notion of an evolutionary trade-off between pre-copulatory signalling displays and sperm production

Migratory Pathways and Connectivity in Asian Houbara Bustards: Evidence from 15 Years of Satellite Tracking

Information on migratory pathways and connectivity is essential to understanding population dynamics and structure of migrant species. Our manuscript uses a unique dataset, the fruit of 103 individual Asian houbara bustards captured on their breeding grounds in Central Asia over 15 years and equipped with satellite transmitters, to provide a better understanding of migratory pathways and connectivity; such information is critical to the implementation of biologically sound conservation measures in migrant species. At the scale of the distribution range we find substantial migratory connectivity, with a clear separation of migration pathways and wintering areas between western and eastern migrants. Within eastern migrants, we also describe a pattern of segregation on the wintering grounds. But at the local level connectivity is weak: birds breeding within the limits of our study areas were often found several hundreds of kilometres apart during winter. Although houbara wintering in Arabia are known to originate from Central Asia, out of all the birds captured and tracked here not one wintered on the Arabian Peninsula. This is very likely the result of decades of unregulated off-take and severe habitat degradation in this area. At a time when conservation measures are being implemented to safeguard the long-term future of this species, this study provides critical data on the spatial structuring of populations