Stochastic theory of non-equilibrium wetting

We study a Langevin equation describing non-equilibrium depinning and wetting transitions. Attention is focused on short-ranged attractive substrate-interface potentials. We confirm the existence of first order depinning transitions, in the temperature-chemical potential diagram, and a tricritical point beyond which the transition becomes a non-equilibrium complete wetting transition. The coexistence of pinned and depinned interfaces occurs over a finite area, in line with other non-equilibrium systems that exhibit first order transitions. In addition, we find two types of phase coexistence, one of which is characterized by spatio-temporal intermittency (STI). A finite size analysis of the depinning time is used to characterize the different coexisting regimes. Finally, a stationary distribution of characteristic triangles or facets was shown to be responsible for the structure of the STI phase.Comment: To appear in Europhys. Lett. // 3 figure

Nonequilibrium wetting transitions with short range forces

We analyze within mean-field theory as well as numerically a KPZ equation that describes nonequilibrium wetting. Both complete and critical wettitng transitions were found and characterized in detail. For one-dimensional substrates the critical wetting temperature is depressed by fluctuations. In addition, we have investigated a region in the space of parameters (temperature and chemical potential) where the wet and nonwet phases coexist. Finite-size scaling analysis of the interfacial detaching times indicates that the finite coexistence region survives in the thermodynamic limit. Within this region we have observed (stable or very long-lived) structures related to spatio-temporal intermittency in other systems. In the interfacial representation these structures exhibit perfect triangular (pyramidal) patterns in one (two dimensions), that are characterized by their slope and size distribution.Comment: 11 pages, 5 figures. To appear in Physical Review

An SMT-based discovery algorithm for C-nets

Recently, Causal nets have been proposed as a suitable model for process discovery, due to their declarative semantics and the great expressiveness they possess. In this paper we propose an algorithm to discover a causal net from a set of traces. It is based on encoding the problem as a Satisfiability Modulo Theories (SMT) formula, and uses a binary search strategy to optimize the derived model. The method has been implemented in a prototype tool that interacts with an SMT solver. The experimental results obtained witness the capability of the approach to discover complex behavior in limited time.Postprint (published version

Definition and Validation of Process Mining Use Cases

Process mining is an emerging topic in the BPM marketplace. Recently, several (commercial) software solutions have become available. Due to the lack of an evaluation framework, it is very difficult for potential users to assess the strengths and weaknesses of these process mining tools. As the first step towards such an evaluation framework, we developed a set of process mining use cases and validated these use cases by means of expert interviews and a survey. We present the list of use cases and discuss the insights from our empirical validation. These use cases will then form the basis for a detailed evaluation of current process mining tools on the market

Conformance checking using activity and trace embeddings

Conformance checking describes process mining techniques used to compare an event log and a corresponding process model. In this paper, we propose an entirely new approach to conformance checking based on neural network-based embeddings. These embeddings are vector representations of every activity/task present in the model and log, obtained via act2vec, a Word2vec based model. Our novel conformance checking approach applies the Word Mover’s Distance to the activity embeddings of traces in order to measure fitness and precision. In addition, we investigate a more efficiently calculated lower bound of the former metric, i.e. the Iterative Constrained Transfers measure. An alternative method using trace2vec, a Doc2vec based model, to train and compare vector representations of the process instances themselves is also introduced. These methods are tested in different settings and compared to other conformance checking techniques, showing promising results

Dextrin-based hydrogel for the development of injectable bone substitute

Book of Abstracts of CEB Annual Meeting 2017[Excerpt] The development of injectable bone substitutes (IBS) have garnered great importance in the bone regeneration field, as a strategy to reach areas of the body using minimally invasive procedures, and showing the ability of perfect fitting according to irregularities of bone tissue defects. In this context, the combination of injectable hydrogels and ceramic granules is emerging as a well-established trend. Particularly, in situ gelation hydrogels have arisen as a new IBS generation. [...]info:eu-repo/semantics/publishedVersio

Experimental Granulomatous Pulmonary Nocardiosis in BALB/C Mice

Pulmonary nocardiosis is a granulomatous disease with high mortality that affects both immunosuppressed and immunocompetent patients. The mechanisms leading to the establishment and progression of the infection are currently unknown. An animal model to study these mechanisms is sorely needed. We report the first in vivo model of granulomatous pulmonary nocardiosis that closely resembles human pathology. BALB/c mice infected intranasally with two different doses of GFP-expressing Nocardia brasiliensis ATCC700358 (NbGFP), develop weight loss and pulmonary granulomas. Mice infected with 109 CFUs progressed towards death within a week while mice infected with 108 CFUs died after five to six months. Histological examination of the lungs revealed that both the higher and lower doses of NbGFP induced granulomas with NbGFP clearly identifiable at the center of the lesions. Mice exposed to 108 CFUs and subsequently to 109 CFUs were not protected against disease severity but had less granulomas suggesting some degree of protection. Attempts to identify a cellular target for the infection were unsuccessful but we found that bacterial microcolonies in the suspension used to infect mice were responsible for the establishment of the disease. Small microcolonies of NbGFP, incompatible with nocardial doubling times starting from unicellular organisms, were identified in the lung as early as six hours after infection. Mice infected with highly purified unicellular preparations of NbGFP did not develop granulomas despite showing weight loss. Finally, intranasal delivery of nocardial microcolonies was enough for mice to develop granulomas with minimal weight loss. Taken together these results show that Nocardia brasiliensis microcolonies are both necessary and sufficient for the development of granulomatous pulmonary nocardiosis in mice

Network analysis of the transcriptional pattern of young and old cells of Escherichia coli during lag phase

Background: The aging process of bacteria in stationary phase is halted if cells are subcultured and enter lag phase and it is then followed by cellular division. Network science has been applied to analyse the transcriptional response, during lag phase, of bacterial cells starved previously in stationary phase for 1 day (young cells) and 16 days (old cells). Results: A genome scale network was constructed for E. coli K-12 by connecting genes with operons, transcription and sigma factors, metabolic pathways and cell functional categories. Most of the transcriptional changes were detected immediately upon entering lag phase and were maintained throughout this period. The lag period was longer for older cells and the analysis of the transcriptome revealed different intracellular activity in young and old cells. The number of genes differentially expressed was smaller in old cells (186) than in young cells (467). Relatively, few genes (62) were up- or down-regulated in both cultures. Transcription of genes related to osmotolerance, acid resistance, oxidative stress and adaptation to other stresses was down-regulated in both young and old cells. Regarding carbohydrate metabolism, genes related to the citrate cycle were up-regulated in young cells while old cells up-regulated the Entner Doudoroff and gluconate pathways and down-regulated the pentose phosphate pathway. In both old and young cells, anaerobic respiration and fermentation pathways were down-regulated, but only young cells up-regulated aerobic respiration while there was no evidence of aerobic respiration in old cells.Numerous genes related to DNA maintenance and replication, translation, ribosomal biosynthesis and RNA processing as well as biosynthesis of the cell envelope and flagellum and several components of the chemotaxis signal transduction complex were up-regulated only in young cells. The genes for several transport proteins for iron compounds were up-regulated in both young and old cells. Numerous genes encoding transporters for carbohydrates and organic alcohols and acids were down-regulated in old cells only. Conclusion: Network analysis revealed very different transcriptional activities during the lag period in old and young cells. Rejuvenation seems to take place during exponential growth by replicative dilution of old cellular components

Combined Treatment of Heterocyclic Analogues and Benznidazole upon Trypanosoma cruzi In Vivo

Chagas disease caused by Trypanosoma cruzi is an important cause of mortality and morbidity in Latin America but no vaccines or safe chemotherapeutic agents are available. Combined therapy is envisioned as an ideal approach since it may enhance efficacy by acting upon different cellular targets, may reduce toxicity and minimize the risk of drug resistance. Therefore, we investigated the activity of benznidazole (Bz) in combination with the diamidine prodrug DB289 and in combination with the arylimidamide DB766 upon T. cruzi infection in vivo. The oral treatment of T.cruzi-infected mice with DB289 and Benznidazole (Bz) alone reduced the number of circulating parasites compared with untreated mice by about 70% and 90%, respectively. However, the combination of these two compounds decreased the parasitemia by 99% and protected against animal mortality by 100%, but without providing a parasitological cure. When Bz (p.o) was combined with DB766 (via ip route), at least a 99.5% decrease in parasitemia levels was observed. DB766+Bz also provided 100% protection against mice mortality while Bz alone provided about 87% protection. This combined therapy also reduced the tissular lesions induced by T. cruzi infection: Bz alone reduced GPT and CK plasma levels by about 12% and 78% compared to untreated mice group, the combination of Bz with DB766 resulted in a reduction of GPT and CK plasma levels of 56% and 91%. Cure assessment through hemocultive and PCR approaches showed that Bz did not provide a parasitological cure, however, DB766 alone or associated with Bz cured ≥13% of surviving animals