941 research outputs found
Contact inhibition of locomotion and mechanical cross-talk between cell-cell and cell-substrate adhesion determines the pattern of junctional tension in epithelial cell aggregates
We generated a computational approach to analyze the biomechanics of
epithelial cell aggregates, either island or stripes or entire monolayers, that
combines both vertex and contact-inhibition-of-locomotion models to include
both cell-cell and cell-substrate adhesion. Examination of the distribution of
cell protrusions (adhesion to the substrate) in the model predicted high order
profiles of cell organization that agree with those previously seen
experimentally. Cells acquired an asymmetric distribution of basal protrusions,
traction forces and apical aspect ratios that decreased when moving from the
edge to the island center. Our in silico analysis also showed that tension on
cell-cell junctions and apical stress is not homogeneous across the island.
Instead, these parameters are higher at the island center and scales up with
island size, which we confirmed experimentally using laser ablation assays and
immunofluorescence. Without formally being a 3-dimensional model, our approach
has the minimal elements necessary to reproduce the distribution of cellular
forces and mechanical crosstalk as well as distribution of principal stress in
cells within epithelial cell aggregates. By making experimental testable
predictions, our approach would benefit the mechanical analysis of epithelial
tissues, especially when local changes in cell-cell and/or cell-substrate
adhesion drive collective cell behavior.Comment: 39 pages, 8 Figures. Supplementary Information is include
A comparison of incompressible limits for resistive plasmas
The constraint of incompressibility is often used to simplify the
magnetohydrodynamic (MHD) description of linearized plasma dynamics because it
does not affect the ideal MHD marginal stability point. In this paper two
methods for introducing incompressibility are compared in a cylindrical plasma
model: In the first method, the limit is taken, where
is the ratio of specific heats; in the second, an anisotropic mass
tensor is used, with the component parallel to the magnetic
field taken to vanish, . Use of resistive MHD reveals
the nature of these two limits because the Alfv\'en and slow magnetosonic
continua of ideal MHD are converted to point spectra and moved into the complex
plane. Both limits profoundly change the slow-magnetosonic spectrum, but only
the second limit faithfully reproduces the resistive Alfv\'en spectrum and its
wavemodes. In ideal MHD, the slow magnetosonic continuum degenerates to the
Alfv\'en continuum in the first method, while it is moved to infinity by the
second. The degeneracy in the first is broken by finite resistivity. For
numerical and semi-analytical study of these models, we choose plasma
equilibria which cast light on puzzling aspects of results found in earlier
literature.Comment: 14 pages, 10 figure
An equilibrium model for RFP plasmas in the presence of resonant tearing modes
The equilibrium of a finite-beta RFP plasma in the presence of
saturated-amplitude tearing modes is investigated. The singularities of the MHD
force balance equation JXB=grad(p) at the modes rational surfaces are resolved
through a proper regularization of the zeroth-order (equilibrium) profiles, by
setting to zero there the gradient of the pressure and parallel current
density. An equilibrium model, which satisfies the regularization rule at the
various rational surfaces, is developed. The comparison with the experimental
data from the Reversed Field eXperiment (RFX) gives encouraging results. The
model provides an easy tool for magnetic analysis: many aspects of the
perturbations can be analyzed and reconstructed.Comment: Final accepted version. 36 page
In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation
Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizing techniques struggle to provide the required sensitivity and accuracy. There is a clear need for the development of new reliable characterisation approaches for the physico-chemical characterisation of nanoparticles with significant accuracy, particularly for the analysis of the particles in the presence of complex biological fluids. Herein, we show that the Differential Centrifugal Sedimentation can be utilised as a high-precision tool for the reliable characterisation of functional nanoparticles of different materials. We report a method to correlate the sedimentation shift with the polymer and biomolecule adsorption on the nanoparticle surface, validating the developed core–shell model. We also highlight its limit when measuring nanoparticles of smaller size and the need to use several complementary methods when characterising nanoparticle corona complexes
Control of neoclassical tearing modes by Sawtooth control
The onset of a neoclassical tearing mode (NTM) depends on the existence of a large enough seed island. It is shown in the Joint European Torus that NTMs can be readily destabilized by long-period sawteeth, such as obtained by sawtooth stabilization from ion-cyclotron heating or current drive. This has important implications for burning plasma scenarios, as alpha particles strongly stabilize the sawteeth. It is also shown that, by adding heating and current drive just outside the inversion radius, sawteeth are destabilized, resulting in shorter sawtooth periods and larger beta values being obtained without NTMs
Aerodynamic investigations of ventilated brake discs.
The heat dissipation and performance of a ventilated brake disc strongly depends
on the aerodynamic characteristics of the flow through the rotor passages. The
aim of this investigation was to provide an improved understanding of ventilated
brake rotor flow phenomena, with a view to improving heat dissipation, as well
as providing a measurement data set for validation of computational fluid
dynamics methods. The flow fields at the exit of four different brake rotor
geometries, rotated in free air, were measured using a five-hole pressure probe
and a hot-wire anemometry system. The principal measurements were taken using
two-component hot-wire techniques and were used to determine mean and unsteady
flow characteristics at the exit of the brake rotors. Using phase-locked data
processing, it was possible to reveal the spatial and temporal flow variation
within individual rotor passages. The effects of disc geometry and rotational
speed on the mean flow, passage turbulence intensity, and mass flow were
determined. The rotor exit jet and wake flow were clearly observed as
characterized by the passage geometry as well as definite regions of high and
low turbulence. The aerodynamic flow characteristics were found to be reasonably
independent of rotational speed but highly dependent upon rotor geometry
BAE dynamics in presence of external magnetic field perturbations in AUG, EAST and JET plasmas
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