Comparative Antioxidant, Antiproliferative and Apoptotic Effects of Ilex laurina and Ilex paraguariensis on Colon Cancer Cells

Purpose: To determine and compare the antioxidant, antiproliferative and apoptotic effects of leaf infusions of Ilex laurina and Ilex paraguariensis in colon cancer cells.Methods: Antioxidant activity was determined by ORAC (Oxygen Radical Absorbance Capacity) and FRAP (Ferric Reducing Antioxidant Power). Cytotoxic and antiproliferative effects were analyzed using MTT ((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and sulfhorodamine-B respectively. Cell death and apoptosis of human colon adenocarcinoma cells SW480 and their metastatic-derived SW620 cells, were analyzed by flow cytometry using propidium iodide and Annexin-V.Results: Although their flavonoid levels were similar, I. laurina infusion contained 2.2 and 4.4 times higher amounts of total phenolic and caffeoyl derivatives, respectively, than I. paraguariensis. FRAP and ORAC values for I. laurina infusion were 1.6 and 2.0 more active than I. paraguariensis. Both plant infusions inhibited viability and cell growth of SW480 and SW620 cells. These results may be associated to cell cycle-arrest and apoptosis because of the comparable increase of hypodiploid and annexin-V positive colon cancer cells.Conclusion: These data highlight the antioxidant and promising anticancer activities of I. laurina and Ilex paraguariensis.Keywords: Ilex laurina, Ilex paraguariensis, Antioxidant, Antiproliferative, Apoptosis, Colon cance

Experimental evidence for inherent Lévy search behaviour in foraging animals

Recently, Lévy walks have been put forward as a new paradigm for animal search and many cases have been made for its presence in nature. However, it remains debated whether Lévy walks are an inherent behavioural strategy or emerge from the animal reacting to its habitat. Here, we demonstrate signatures of Lévy behaviour in the search movement of mud snails (Hydrobia ulvae) based on a novel, direct assessment of movement properties in an experimental set-up using different food distributions. Our experimental data uncovered clusters of small movement steps alternating with long moves independent of food encounter and landscape complexity. Moreover, size distributions of these clusters followed truncated power laws. These two findings are characteristic signatures of mechanisms underlying inherent Lévy-like movement. Thus, our study provides clear experimental evidence that such multi-scale movement is an inherent behaviour rather than resulting from the animal interacting with its environmen

Effect of Anesthesia on Microelectrode Recordings during Deep Brain Stimulation Surgery in Tourette Syndrome Patients

Background: Deep brain stimulation (DBS) is an accepted treatment for patients with medication-resistant Tourette syndrome (TS). Sedation is commonly required during electrode implantation to attenuate anxiety, pain, and severe tics. Anesthetic agents potentially impair the quality of microelectrode recordings (MER). Little is known about the effect of these anesthetics on MER in patients with TS. We describe our experience with different sedative regimens on MER and tic severity in patients with TS. Methods: The clinical records of all TS patients who underwent DBS surgery between 2010 and 2018 were reviewed. Demographic data, stimulation targets, anesthetic agents, perioperative complications, and MER from each hemisphere were collected and analyzed. Single-unit activity was identified by filtering spiking activity from broadband MER data and principal component analysis with K-means clustering. Vocal and motor tics which caused artifacts in the MER data were manually selected using visual and auditory inspection. Results: Six patients underwent bilateral DBS electrode implantation. In all patients, the target was the anterior internal globus pallidus. Patient comfort and hemodynamic and respiratory stability were maintained with conscious sedation with one or more of the following anesthetic drugs: propofol, midazolam, remifentanil, clonidine, and dexmedetomidine. Good quality MER and clinical testing were obtained in 9 hemispheres of 6 patients. In 3 patients, MER quality was poor on one side. Conclusion: Cautiously applied sedative drugs can provide patient comfort, hemodynamic and respiratory stability, and suppress severe tics, with minimal interference with MER. (C) 2019 The Author(s) Published by S. Karger AG, Base

Evolution of Class IITCPgenes in perianth bearing Piperales and their contribution to the bilateral calyx in Aristolochia

[EN] Controlled spatiotemporal cell division and expansion are responsible for floral bilateral symmetry. Genetic studies have pointed to class II TCP genes as major regulators of cell division and floral patterning in model core eudicots. Here we study their evolution in perianth-bearing Piperales and their expression in Aristolochia, a rare occurrence of bilateral perianth outside eudicots and monocots. The evolution of class II TCP genes reveals single-copy CYCLOIDEA-like genes and three paralogs of CINCINNATA (CIN) in early diverging angiosperms. All class II TCP genes have independently duplicated in Aristolochia subgenus Siphisia. Also CIN2 genes duplicated before the diversification of Saruma and Asarum. Sequence analysis shows that CIN1 and CIN3 share motifs with Cyclin proteins and CIN2 genes have lost the miRNA319a binding site. Expression analyses of all paralogs of class II TCP genes in Aristolochia fimbriata point to a role of CYC and CIN genes in maintaining differential perianth expansion during mid- and late flower developmental stages by promoting cell division in the distal and ventral portion of the limb. It is likely that class II TCP genes also contribute to cell division in the leaf, the gynoecium and the ovules in A. fimbriata.We thank Anny Garces Palacio, Sarita Munoz, Pablo Perez-Mesa (Universidad de Antioquia, Colombia), Cecilia Zumajo-Cardona (The New York Botanical Garden), Ana Berbel and Clara Ines Ortiz-Ramirez (Instituto de Biologia Molecular y Celular de Plantas, CSIC-UVP, Valencia, Spain) for photographs and assistance during laboratory work. We also thank Sebastian Gonzalez (Massachusetts College of Art and Design) for taking some of the photographs in Figs 1 and 2. Thanks are also due to the Dresden Junior Fellowship for allowing the visiting professor fellowship of NPM to the Technishe Universitat Dresden during 2019. This research was funded by Estrategia de Sostenibilidad 2018-2019 the Convocatoria Programaticas 2017-2018 (code 2017-16302), and the 2018-2019 Fondo de Internacionalizacion (code 201926230) from the Universidad de Antioquia, the iCOOP + 2016 grant COOPB20250 from Centro Superior de Investigacion Cientifica, CSIC and the ExpoSEED (H2020.MSCA-RISE2015-691109) EU grant.Pabon-Mora, N.; Madrigal, Y.; Alzate, JF.; Ambrose, BA.; Ferrandiz Maestre, C.; Wanke, S.; Neinhuis, C.... (2020). Evolution of Class IITCPgenes in perianth bearing Piperales and their contribution to the bilateral calyx in Aristolochia. New Phytologist. 228(2):752-769. https://doi.org/10.1111/nph.167197527692282Aguilar-Martínez, J. A., Poza-Carrión, C., & Cubas, P. (2007). Arabidopsis BRANCHED1Acts as an Integrator of Branching Signals within Axillary Buds. The Plant Cell, 19(2), 458-472. doi:10.1105/tpc.106.048934Almeida, J., Rocheta, M., & Galego, L. (1997). Genetic control of flower shape in Antirrhinum majus. Development, 124(7), 1387-1392. doi:10.1242/dev.124.7.1387Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403-410. doi:10.1016/s0022-2836(05)80360-2Ambrose, B. A., Lerner, D. R., Ciceri, P., Padilla, C. M., Yanofsky, M. F., & Schmidt, R. J. (2000). Molecular and Genetic Analyses of the Silky1 Gene Reveal Conservation in Floral Organ Specification between Eudicots and Monocots. Molecular Cell, 5(3), 569-579. doi:10.1016/s1097-2765(00)80450-5Ballester, P., Navarrete-Gómez, M., Carbonero, P., Oñate-Sánchez, L., & Ferrándiz, C. (2015). Leaf expansion in Arabidopsis is controlled by a TCP-NGA regulatory module likely conserved in distantly related species. Physiologia Plantarum, 155(1), 21-32. doi:10.1111/ppl.12327Bartlett, M. E., & Specht, C. D. (2011). Changes in expression pattern of the teosinte branched1- like genes in the Zingiberales provide a mechanism for evolutionary shifts in symmetry across the order. American Journal of Botany, 98(2), 227-243. doi:10.3732/ajb.1000246Bliss, B. J., Wanke, S., Barakat, A., Ayyampalayam, S., Wickett, N., Wall, P. K., … dePamphilis, C. W. (2013). Characterization of the basal angiosperm Aristolochia fimbriata: a potential experimental system for genetic studies. BMC Plant Biology, 13(1), 13. doi:10.1186/1471-2229-13-13Busch, A., & Zachgo, S. (2007). Control of corolla monosymmetry in the Brassicaceae Iberis amara. Proceedings of the National Academy of Sciences, 104(42), 16714-16719. doi:10.1073/pnas.0705338104Citerne, H. L., Reyes, E., Le Guilloux, M., Delannoy, E., Simonnet, F., Sauquet, H., … Damerval, C. (2016). Characterization ofCYCLOIDEA-like genes in Proteaceae, a basal eudicot family with multiple shifts in floral symmetry. Annals of Botany, 119(3), 367-378. doi:10.1093/aob/mcw219Corley, S. B., Carpenter, R., Copsey, L., & Coen, E. (2005). Floral asymmetry involves an interplay between TCP and MYB transcription factors in Antirrhinum. Proceedings of the National Academy of Sciences, 102(14), 5068-5073. doi:10.1073/pnas.0501340102Crawford, B. C. W., Nath, U., Carpenter, R., & Coen, E. S. (2004). CINCINNATA Controls Both Cell Differentiation and Growth in Petal Lobes and Leaves of Antirrhinum. Plant Physiology, 135(1), 244-253. doi:10.1104/pp.103.036368Cubas, P. (2002). Role of TCP genes in the evolution of morphological characters in angiosperms. Developmental Genetics and Plant Evolution, 247-266. doi:10.1201/9781420024982.ch13Cubas, P., Lauter, N., Doebley, J., & Coen, E. (1999). The TCP domain: a motif found in proteins regulating plant growth and development. The Plant Journal, 18(2), 215-222. doi:10.1046/j.1365-313x.1999.00444.xDamerval, C., Citerne, H., Conde e Silva, N., Deveaux, Y., Delannoy, E., Joets, J., … Nadot, S. (2019). Unraveling the Developmental and Genetic Mechanisms Underpinning Floral Architecture in Proteaceae. Frontiers in Plant Science, 10. doi:10.3389/fpls.2019.00018Damerval, C., Citerne, H., Le Guilloux, M., Domenichini, S., Dutheil, J., Ronse de Craene, L., & Nadot, S. (2013). Asymmetric morphogenetic cues along the transverse plane: Shift from disymmetry to zygomorphy in the flower of Fumarioideae. American Journal of Botany, 100(2), 391-402. doi:10.3732/ajb.1200376Damerval, C., Guilloux, M. L., Jager, M., & Charon, C. (2006). Diversity and Evolution ofCYCLOIDEA-Like TCP Genes in Relation to Flower Development in Papaveraceae. Plant Physiology, 143(2), 759-772. doi:10.1104/pp.106.090324Danisman, S., van der Wal, F., Dhondt, S., Waites, R., de Folter, S., Bimbo, A., … Immink, R. G. H. (2012). Arabidopsis Class I and Class II TCP Transcription Factors Regulate Jasmonic Acid Metabolism and Leaf Development Antagonistically. Plant Physiology, 159(4), 1511-1523. doi:10.1104/pp.112.200303Danisman, S., van Dijk, A. D. J., Bimbo, A., van der Wal, F., Hennig, L., de Folter, S., … Immink, R. G. H. (2013). Analysis of functional redundancies within the Arabidopsis TCP transcription factor family. Journal of Experimental Botany, 64(18), 5673-5685. doi:10.1093/jxb/ert337Doebley, J. (2004). The Genetics of Maize Evolution. Annual Review of Genetics, 38(1), 37-59. doi:10.1146/annurev.genet.38.072902.092425Doebley, J., Stec, A., & Gustus, C. (1995). teosinte branched1 and the origin of maize: evidence for epistasis and the evolution of dominance. Genetics, 141(1), 333-346. doi:10.1093/genetics/141.1.333Doebley, J., Stec, A., & Hubbard, L. (1997). The evolution of apical dominance in maize. Nature, 386(6624), 485-488. doi:10.1038/386485a0Efroni, I., Blum, E., Goldshmidt, A., & Eshed, Y. (2008). A Protracted and Dynamic Maturation Schedule UnderliesArabidopsisLeaf Development. The Plant Cell, 20(9), 2293-2306. doi:10.1105/tpc.107.057521Elomaa, P., Zhao, Y., & Zhang, T. (2018). Flower heads in Asteraceae—recruitment of conserved developmental regulators to control the flower-like inflorescence architecture. Horticulture Research, 5(1). doi:10.1038/s41438-018-0056-8Endress, P. K. (2012). The Immense Diversity of Floral Monosymmetry and Asymmetry Across Angiosperms. The Botanical Review, 78(4), 345-397. doi:10.1007/s12229-012-9106-3Ferrándiz, C., Liljegren, S. J., & Yanofsky, M. F. (2000). Negative Regulation of the SHATTERPROOF Genes by FRUITFULL During Arabidopsis Fruit Development. Science, 289(5478), 436-438. doi:10.1126/science.289.5478.436Galego, L. (2002). Role of DIVARICATA in the control of dorsoventral asymmetry in Antirrhinum flowers. Genes & Development, 16(7), 880-891. doi:10.1101/gad.221002Gaudin, V., Lunness, P. A., Fobert, P. R., Towers, M., Riou-Khamlichi, C., Murray, J. A. H., … Doonan, J. H. (2000). The Expression of D-Cyclin Genes Defines Distinct Developmental Zones in Snapdragon Apical Meristems and Is Locally Regulated by the Cycloidea Gene. Plant Physiology, 122(4), 1137-1148. doi:10.1104/pp.122.4.1137González, F., & Pabón‐Mora, N. (2015). Trickery flowers: the extraordinary chemical mimicry of Aristolochia to accomplish deception to its pollinators. New Phytologist, 206(1), 10-13. doi:10.1111/nph.13328González, F., & Stevenson, D. W. (2000). Perianth development and systematics of Aristolochia. Flora, 195(4), 370-391. doi:10.1016/s0367-2530(17)30995-7Heery, D. M., Kalkhoven, E., Hoare, S., & Parker, M. G. (1997). A signature motif in transcriptional co-activators mediates binding to nuclear receptors. Nature, 387(6634), 733-736. doi:10.1038/42750Hileman, L. C. (2014). Trends in flower symmetry evolution revealed through phylogenetic and developmental genetic advances. Philosophical Transactions of the Royal Society B: Biological Sciences, 369(1648), 20130348. doi:10.1098/rstb.2013.0348Hoang, D. T., Chernomor, O., von Haeseler, A., Minh, B. Q., & Vinh, L. S. (2017). UFBoot2: Improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution, 35(2), 518-522. doi:10.1093/molbev/msx281Horn, S., Pabón-Mora, N., Theuß, V. S., Busch, A., & Zachgo, S. (2015). Analysis of the CYC/TB1 class of TCP transcription factors in basal angiosperms and magnoliids. The Plant Journal, 81(4), 559-571. doi:10.1111/tpj.12750Howarth, D. G., & Donoghue, M. J. (2006). Phylogenetic analysis of the «ECE» (CYC/TB1) clade reveals duplications predating the core eudicots. Proceedings of the National Academy of Sciences, 103(24), 9101-9106. doi:10.1073/pnas.0602827103Howarth, D. G., Martins, T., Chimney, E., & Donoghue, M. J. (2011). Diversification of CYCLOIDEA expression in the evolution of bilateral flower symmetry in Caprifoliaceae and Lonicera (Dipsacales). Annals of Botany, 107(9), 1521-1532. doi:10.1093/aob/mcr049Kalyaanamoorthy, S., Minh, B. Q., Wong, T. K. F., von Haeseler, A., & Jermiin, L. S. (2017). ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14(6), 587-589. doi:10.1038/nmeth.4285Katoh, K. (2002). MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30(14), 3059-3066. doi:10.1093/nar/gkf436Kosugi, S., & Ohashi, Y. (2002). DNA binding and dimerization specificity and potential targets for the TCP protein family. The Plant Journal, 30(3), 337-348. doi:10.1046/j.1365-313x.2002.01294.xKoyama, T., Furutani, M., Tasaka, M., & Ohme-Takagi, M. (2006). TCP Transcription Factors Control the Morphology of Shoot Lateral Organs via Negative Regulation of the Expression of Boundary-Specific Genes inArabidopsis. The Plant Cell, 19(2), 473-484. doi:10.1105/tpc.106.044792Leppik, E. E. (1972). Origin and Evolution of Bilateral Symmetry in Flowers. Evolutionary Biology, 49-85. doi:10.1007/978-1-4757-0256-9_3Li, C., Potuschak, T., Colon-Carmona, A., Gutierrez, R. A., & Doerner, P. (2005). Arabidopsis TCP20 links regulation of growth and cell division control pathways. Proceedings of the National Academy of Sciences, 102(36), 12978-12983. doi:10.1073/pnas.0504039102Li, M., Zhang, D., Gao, Q., Luo, Y., Zhang, H., Ma, B., … Xue, Y. (2019). Genome structure and evolution of Antirrhinum majus L. Nature Plants, 5(2), 174-183. doi:10.1038/s41477-018-0349-9Li, S. (2015). The Arabidopsis thaliana TCP transcription factors: A broadening horizon beyond development. Plant Signaling & Behavior, 10(7), e1044192. doi:10.1080/15592324.2015.1044192Li, S., Gutsche, N., & Zachgo, S. (2011). The ROXY1 C-Terminal L**LL Motif Is Essential for the Interaction with TGA Transcription Factors    . Plant Physiology, 157(4), 2056-2068. doi:10.1104/pp.111.185199Lin, Y.-F., Chen, Y.-Y., Hsiao, Y.-Y., Shen, C.-Y., Hsu, J.-L., Yeh, C.-M., … Tsai, W.-C. (2016). Genome-wide identification and characterization ofTCPgenes involved in ovule development ofPhalaenopsis equestris. Journal of Experimental Botany, 67(17), 5051-5066. doi:10.1093/jxb/erw273Da Luo, Carpenter, R., Copsey, L., Vincent, C., Clark, J., & Coen, E. (1999). Control of Organ Asymmetry in Flowers of Antirrhinum. Cell, 99(4), 367-376. doi:10.1016/s0092-8674(00)81523-8Luo, D., Carpenter, R., Vincent, C., Copsey, L., & Coen, E. (1996). Origin of floral asymmetry in Antirrhinum. Nature, 383(6603), 794-799. doi:10.1038/383794a0Madrigal, Y., Alzate, J. F., & Pabón-Mora, N. (2017). Evolution and Expression Patterns of TCP Genes in Asparagales. Frontiers in Plant Science, 8. doi:10.3389/fpls.2017.00009Martín-Trillo, M., & Cubas, P. (2010). TCP genes: a family snapshot ten years later. Trends in Plant Science, 15(1), 31-39. doi:10.1016/j.tplants.2009.11.003MillerMA PfeifferW SchwartzT.2010.Creating the CIPRES Science Gateway for inference of large phylogenetic trees. [WWW document] URLhttp://www.phylo.org[accessed 5 June 2020].Mondragón-Palomino, M., & Trontin, C. (2011). High time for a roll call: gene duplication and phylogenetic relationships of TCP-like genes in monocots. Annals of Botany, 107(9), 1533-1544. doi:10.1093/aob/mcr059Nath, U., Crawford, B. C. W., Carpenter, R., & Coen, E. (2003). Genetic Control of Surface Curvature. Science, 299(5611), 1404-1407. doi:10.1126/science.1079354Navaud, O., Dabos, P., Carnus, E., Tremousaygue, D., & Hervé, C. (2007). TCP Transcription Factors Predate the Emergence of Land Plants. Journal of Molecular Evolution, 65(1), 23-33. doi:10.1007/s00239-006-0174-zNguyen, L.-T., Schmidt, H. A., von Haeseler, A., & Minh, B. Q. (2014). IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies. Molecular Biology and Evolution, 32(1), 268-274. doi:10.1093/molbev/msu300Pabón-Mora, N., Suárez-Baron, H., Ambrose, B. A., & González, F. (2015). Flower Development and Perianth Identity Candidate Genes in the Basal Angiosperm Aristolochia fimbriata (Piperales: Aristolochiaceae). Frontiers in Plant Science, 6. doi:10.3389/fpls.2015.01095Palatnik, J. F., Allen, E., Wu, X., Schommer, C., Schwab, R., Carrington, J. C., & Weigel, D. (2003). Control of leaf morphogenesis by microRNAs. Nature, 425(6955), 257-263. doi:10.1038/nature01958Parapunova, V., Busscher, M., Busscher-Lange, J., Lammers, M., Karlova, R., Bovy, A. G., … de Maagd, R. A. (2014). Identification, cloning and characterization of the tomato TCP transcription factor family. BMC Plant Biology, 14(1). doi:10.1186/1471-2229-14-157Peréz-Mesa, P., Ortíz-Ramírez, C. I., González, F., Ferrándiz, C., & Pabón-Mora, N. (2020). Expression of gynoecium patterning transcription factors in Aristolochia fimbriata (Aristolochiaceae) and their contribution to gynostemium development. EvoDevo, 11(1). doi:10.1186/s13227-020-00149-8Preston, J. C., & Hileman, L. C. (2012). Parallel evolution of TCP and B-class genes in Commelinaceae flower bilateral symmetry. EvoDevo, 3(1), 6. doi:10.1186/2041-9139-3-6Preston, J. C., Kost, M. A., & Hileman, L. C. (2009). Conservation and diversification of the symmetry developmental program among close relatives of snapdragon with divergent floral morphologies. New Phytologist, 182(3), 751-762. doi:10.1111/j.1469-8137.2009.02794.xRambautA.2014.FigTree: tree figure drawing tool. [WWW document] URLhttp://tree.bio.ed.ac.uk/software/figtree/.Rudall, P. J., & Bateman, R. M. (2004). Evolution of zygomorphy in monocot flowers: iterative patterns and developmental constraints. New Phytologist, 162(1), 25-44. doi:10.1111/j.1469-8137.2004.01032.xSargent, R. D. (2004). Floral symmetry affects speciation rates in angiosperms. Proceedings of the Royal Society of London. Series B: Biological Sciences, 271(1539), 603-608. doi:10.1098/rspb.2003.2644Suárez-Baron, H., Alzate, J. F., González, F., Ambrose, B. A., & Pabón-Mora, N. (2019). Genetic mechanisms underlying perianth epidermal elaboration of Aristolochia ringens Vahl (Aristolochiaceae). Flora, 253, 56-66. doi:10.1016/j.flora.2019.03.004Suárez-Baron, H., Pérez-Mesa, P., Ambrose, B. A., González, F., & Pabón-Mora, N. (2016). Deep into the Aristolochia Flower: Expression of C, D, and E-Class Genes inAristolochia fimbriata(Aristolochiaceae). Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 328(1-2), 55-71. doi:10.1002/jez.b.22686Viola, I. L., Uberti Manassero, N. G., Ripoll, R., & Gonzalez, D. H. (2011). The Arabidopsis class I TCP transcription factor AtTCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain. Biochemical Journal, 435(1), 143-155. doi:10.1042/bj20101019Wang, J., Wang, Y., & Luo, D. (2010). LjCYC Genes Constitute Floral Dorsoventral Asymmetry in Lotus japonicus. Journal of Integrative Plant Biology, 52(11), 959-970. doi:10.1111/j.1744-7909.2010.00926.xYuan, Z., Gao, S., Xue, D.-W., Luo, D., Li, L.-T., Ding, S.-Y., … Zhang, D.-B. (2008). RETARDED PALEA1 Controls Palea Development and Floral Zygomorphy in Rice  . Plant Physiology, 149(1), 235-244. doi:10.1104/pp.108.128231Zhang, W., Kramer, E. M., & Davis, C. C. (2010). Floral symmetry genes and the origin and maintenance of zygomorphy in a plant-pollinator mutualism. Proceedings of the National Academy of Sciences, 107(14), 6388-6393. doi:10.1073/pnas.0910155107Zhang, W., Steinmann, V. W., Nikolov, L., Kramer, E. M., & Davis, C. C. (2013). Divergent genetic mechanisms underlie reversals to radial floral symmetry from diverse zygomorphic flowered ancestors. Frontiers in Plant Science, 4. doi:10.3389/fpls.2013.0030

Modelización de procesos de enseñanza en profesores de ciencias de la ciudad de manizales (colombia) desde el concepto contenido pedagógico del conocimiento

Se modelizó el pensamiento de 50 profesores de educación básica secundaria sobre el concepto Contenido Pedagógico del Conocimiento. El análisis de la información recolectada se realizó con el empleo de Atlas-Ti. Los maestros reconocen que para enseñar ciencias no es suficiente con tener el conocimiento de la materia; también consideran necesarios otros tipos de conocimientos como el pedagógico general, de los estudiantes, curricular, del contexto y didáctico. Los docentes consideran esencial para enseñar ciencias el empleo de múltiples estrategias en el proceso de enseñanza, despertar el interés de los estudiantes y tener conocimientos adecuados sobre el manejo de los grupos de clase

Hydrodynamic Study in a Cone Bottom Stirred Tank Using Computational Fluid Dynamics

Stirred tanks are often used in industrial applications to store and process liquids and solids. However, these systems have become an increasing challenge to improve and optimize these processes. Computational Fluids Dynamics (CFD) simulation predicts complex phenomena as hydrodynamics system performance. An optimal solution is found using an effective mesh scheme and selecting appropriate boundary conditions. This work aims to validate and describe the distribution velocities inside the tank using a rigorous turbulence model. Stirred tank with a diameter of 27 cm and an oval cone tip using a Rushton impeller (radial impeller) and a 4-blade impeller inclined at 45° (axial impeller) are performed. For both cases, hydrodynamics in the bottom tank is analyzed. In addition, the power and the pumping numbers for each impeller are studied. The overall results show that at the tip of the oval cone, the asymmetry in the mesh is improved, and the divergence in the solution is avoided. Also, the cone designer increased the turbulent kinetic energy, which can enhance the mixture process. A decrease in power impeller is shown when the axial type is applied at low Reynolds numbers; however, when the cone is introduced inside the tank and a radial impeller type is used, the impeller power values are increased. The overall results of CFD simulation are compared to experimental data and provide similar values with an absolute deviation below 4.46 %

Characterization of mammary neoplasia by electrical impedance spectroscopy: canine model

Introducción: La espectroscopia de impedancia eléctrica (EIE) es una técnica fácil de usar y de bajo costo que se puede utilizar para analizar tejidos biológicos en condiciones normales o patológicas. El objetivo de este trabajo fue caracterizar neoplasias de glándula mamaria benignas y malignas aplicando la técnica EIE en muestras extraídas de 45 caninos hembras (Canis lupus familiaris). Métodos: Se utilizó un medidor de impedancia eléctrica, Hioki 3532-50, para determinar los parámetros bioeléctricos: resistencia de la matriz extracelular (R), resistencia de la matriz intracelular (S), frecuencia característica (Fc) y capacitancia de membrana (Cm) en un rango de frecuencias entre 42 Hz y 5 MHz y se analizaron estadísticamente mediante la prueba no paramétrica U de Mann-Whitney (Wilcoxon) de dos colas. La precisión diagnóstica de la EIE se efectuó a través de curvas características de operación del receptor (COR) y tablas de doble entrada, con la histopatología como referencia. Resultados: Se encontraron diferencias estadísticamente significativas entre el tejido mamario sano y las neoplasias benignas para los parámetros R, Fc y Cm, p-value < 0,05. Entre tejido mamario sano y neoplasias mamarias malignas se encontraron diferencias estadísticamente significativas para R y Fc con un p-value < 0,05. La comparación entre lesiones tumorales benignas y malignas no presentó diferencias estadísticamente significativas, p-value > 0,05, para ninguna de las variables incluidas en este estudio. Conclusiones: De los parámetros analizados por EIE, la resistencia de la matriz extracelular es la que mejor permite diferenciar entre tejidos mamarios normales y neoplásicos. La EIE es una herramienta diagnóstica potencial que puede ser utilizada en la detección de cáncer mamario, con una precisión diagnóstica cercana al 80%.Introduction: Electrical Impedance Spectroscopy (EIS) it is an easy to use and low-cost technique that can be used to analyze biological tissues in normal or pathological condition. The goal of this work was to characterize benign and malign mammary gland neoplasms applying the EIS technique in 45 female dogs (Canis lupus familiaris). Methods: An impedance meter Hioki 3532-50 was used to determine bioelectric parameters, extracellular matrix resistance (R), intracellular matrix resistance (S), characteristic frequency (Cf), and membrane capacitance (Mc), which were obtained in a 42 Hz and 5 MHz frequencies range. Were statistically analyzed with the non-parametric test of two-tailed MannWhitney (Wilcoxon). The diagnostic precision of the test was performed using receiver operating characteristics (ROC) and two-way tables using histopathology results as reference. Results: Significant differences between healthy mammary tissue and benign neoplasms were found for variables R, Cf and Mc (p < 0.05). There were statistically major differences between the healthy mammary tissue and malign mammary tumors groups for R and Cf (p < 0.05). The comparison between malign and benign tumor lesions did not show a statistically significant difference, p-value > 0.05, for any of the variables included in this study. Conclusion: Among all parameters analyzed for EIS, the extracellular matrix resistance R is the one that best allows differentiating between healthy and neoplastic mammary tissues. EIS is a diagnostic tool that can be used for breast cancer detection with a diagnostic precision close to 80%

Influence of Anesthesia and Clinical Variables on the Firing Rate, Coefficient of Variation and Multi-Unit Activity of the Subthalamic Nucleus in Patients with Parkinson's Disease

BACKGROUND: Microelectrode recordings (MER) are used to optimize lead placement during subthalamic nucleus deep brain stimulation (STN-DBS). To obtain reliable MER, surgery is usually performed while patients are awake. Procedural sedation and analgesia (PSA) is often desirable to improve patient comfort, anxiolysis and pain relief. The effect of these agents on MER are largely unknown. The objective of this study was to determine the effects of commonly used PSA agents, dexmedetomidine, clonidine and remifentanil and patient characteristics on MER during DBS surgery. METHODS: Data from 78 patients with Parkinson's disease (PD) who underwent STN-DBS surgery were retrospectively reviewed. The procedures were performed under local anesthesia or under PSA with dexmedetomidine, clonidine or remifentanil. In total, 4082 sites with multi-unit activity (MUA) and 588 with single units were acquired. Single unit firing rates and coefficient of variation (CV), and MUA total power were compared between patient groups. RESULTS: We observed a significant reduction in MUA, an increase of the CV and a trend for reduced firing rate by dexmedetomidine. The effect of dexmedetomidine was dose-dependent for all measures. Remifentanil had no effect on the firing rate but was associated with a significant increase in CV and a decrease in MUA. Clonidine showed no significant effect on firing rate, CV or MUA. In addition to anesthetic effects, MUA and CV were also influenced by patient-dependent variables. CONCLUSION: Our results showed that PSA influenced neuronal properties in the STN and the dexmedetomidine (DEX) effect was dose-dependent. In addition, patient-dependent characteristics also influenced MER

A specific structure and high richness characterize intestinal microbiota of HIVexposed seronegative individuals

Intestinal microbiota facilitates food breakdown for energy metabolism and influences the im-mune response and maintaining mucosal homeostasis. Overall, HIV infection is associated with intestinal dysbiosis and immune activation, which has been related to seroconversion in HIV-exposed individuals. However, to date, it is unclear whether microbiota dysbiosis is the cause or the effect of immune alterations and disease progression. We characterize the intestinal microbiota and determine its association with immune regulation in HIV-exposed seronegative individuals (HESN), HIV-infected progressors (HIV+), and healthy control (HC) subjects. For this, feces and blood were collected. The microbiota composition of HESN showed a significantly higher alpha and beta diversity compared to HC, but similar to HIV+. A lower Treg percentage was observed in HESN than HC and HIV+, with enrichment of the genus Butyrivibrio being characteristic of this profile. Interestingly, an increase in Succinivibrio and Prevotella and a re-duction in Bacteroides genus were observed in HESN compared to HC, which is typical of HIV-infected individuals. Thus, HESNs have a microbiota profile, similar to that observed in HIV+, most likely because HESN are cohabiting with their HIV+ partners.Intestinal microbiota facilitates food breakdown for energy metabolism and influences the im-mune response and maintaining mucosal homeostasis. Overall, HIV infection is associated with intestinal dysbiosis and immune activation, which has been related to seroconversion in HIV-exposed individuals. However, to date, it is unclear whether microbiota dysbiosis is the cause or the effect of immune alterations and disease progression. We characterize the intestinal microbiota and determine its association with immune regulation in HIV-exposed seronegative individuals (HESN), HIV-infected progressors (HIV+), and healthy control (HC) subjects. For this, feces and blood were collected. The microbiota composition of HESN showed a significantly higher alpha and beta diversity compared to HC, but similar to HIV+. A lower Treg percentage was observed in HESN than HC and HIV+, with enrichment of the genus Butyrivibrio being characteristic of this profile. Interestingly, an increase in Succinivibrio and Prevotella and a re-duction in Bacteroides genus were observed in HESN compared to HC, which is typical of HIV-infected individuals. Thus, HESNs have a microbiota profile, similar to that observed in HIV+, most likely because HESN are cohabiting with their HIV+ partners.https://scienti.minciencias.gov.co/cvlac/EnProdArticulo/query.do?cod_producto=73&cod_rh=0000157775https://scholar.google.com.co/citations?hl=en&user=VLZxl1UAAAAJCOL0112548https://orcid.org/0000-0002-7351-873