Tear proteomic profiling with MALDI-TOF mass spectrometry coupled with magnetic-beads HIC-18 separation as a diagnostic tools in blepharitis disease

Comunicaciones a congreso

Oral bacterial microflora associated with total acrylic dentures: implant supported vs mucus supported

Poster presented at the 1st International Congress of CiiEM - From Basic Sciences to Clinical Research. 27-28 November 2015, Egas Moniz, Caparica, Portugal

Performance of fly ash-based geopolymer mortar

This study has investigated the joint effect of several mix parameters on the properties of foam geopolymers. The mix parameters analysed through a laboratory experiment of 54 different mortar mixes were, sodium silicate/sodium hydroxide mass ratio (2.5, 3.5, 4.5), activator/binder mass ratio (0.6, 0.8, 1.0), chemical foaming agent type (hydrogen peroxide (H2O2) and sodium perborate (NaBO3)) and foaming agent mass ratio content (1%, 2%, 3%). Properties, SEM and FTIR analysis and cost analysis are included. The results show that the sodium perborate over performs hydrogen peroxide leading to a lower overall thermal conductibility of foam geopolymers. The use of an activator/binder ratio of 0.8 a sodium silicate/sodium hydroxide of 2.5 led to the lowest thermal conductivity. Mixtures with a low thermal conductivity of around 0.1 W/(m.ºK) and a compressive strength of around 6 MPa were achieved. The cost analysis show that the foaming agents are responsible for a small percentage of foam geopolymers total cost (less than 10%) being that the alkaline activators are responsible for more than 80

Effect of extrusion on the trypsin inhibitors activity and digestibility of the raw soybean in dogs determined by the regression, substitution, and difference methods.

The lack of standardized methodologies to evaluate an ingredient?s digestibility in dogs contributes to the poor information about it. This is particularly important in protein sources, such as soybean, because dogs are carnivorous. There has been little investigation on the effect of pet food extrusion on the inactivation of trypsin inhibitors present in raw soybeans (RSB). Because of this, we aimed to evaluate the effect of extrusion on trypsin inhibitors and on the digestibility of RSB by the regression (REG), substitution (SUB), and difference (DIF) methods in dogs. Animals, material and methods: Six adult Beagle dogs were distributed in a Latin square design (6 x 6). The dogs were fed six extruded diets (single screw extruder) with increasing levels of RSB (0, 6, 12, 18, 24, and 30%) for a 5-day adaptation period followed by 5 days of total faecal collection per period (AAFCO, 2004). Trypsin inhibitors activity was determined in the diets before and after extrusion. The coefficients of total tract apparent digestibility (CTTAD) of nutrients and the metabolizable energy (ME) of the RSB were determined by the SUB (Matterson et al., 1965), REG, and DIF methods (Fan and Sauer, 1995). Data were submitted to regression and to analysis of variance and the means were compared by Tukey's test (P0.05). The ether extract CTTAD (0.891 to 0.910) and the ME (18.4 to 19.0 MJ/kg) of the diets increased linearly (P0.05) in the CTTAD and ME predicted by the three methods, nor between the variances of the methods. Conclusion: The single screw extrusion process of diets containing high levels of RSB does not completely inactivate the trypsin inhibitors. However, up to 12% of RSB can be used in extruded diets without compromising protein digestibility and faecal consistency of dogs. Greater inclusion levels of RSB reduce estimative errors of this ingredient digestibility. Although the three methods can be used to predict ingredients digestibility in dogs, the DIF and the SUB methods are more practical than the REG method, considering the utilization of only two diets (reference and test), while the REG method requires at least four diets

Mix design, properties and cost analysis of fly ash-based geopolymer foam

This study has investigated the joint effect of several mix parameters on the properties of foam geopolymers. The mix parameters analysed through a laboratory experiment of 54 different mortar mixes were, sodium silicate/sodium hydroxide mass ratio (2.5, 3.5, 4.5), activator/binder mass ratio (0.6, 0.8, 1.0), chemical foaming agent type (hydrogen peroxide (H2O2) and sodium perborate (NaBO3)) and foaming agent mass ratio content (1%, 2%, 3%). Properties, SEM and FTIR analysis and cost analysis are included. The results show that the sodium perborate over performs hydrogen peroxide leading to a lower overall thermal conductibility of foam geopolymers. Mixtures with a low thermal conductivity of around 0.1 W/ (m K) and a compressive strength of around 6 MPa were achieved. The cost analysis show that the foaming agents are responsible for a small percentage of foam geopolymers total cost being that the alkaline activators are responsible for more than 80%

Morphological and Molecular Characterization of Grapevine Accessions Known as Albillo

Twenty-eight accessions identified under the name of Albillo or cited as a synonym of this ancient Spanish grapevine variety were characterized using 43 morphological descriptors, four different isozyme systems (CO, ACPH, GOT, and SOD) and two Amplified Fragment Length Polymorphism (AFLP) primer combinations. Genetic similarity data resulting from these analyses allowed three different groups of Albillo to be distinguished that corresponded with geographical and historical origin of representative varieties: Turruntés, Malvasía de El Bierzo, and the classical Albillo de Madrid, related to the oldest Albillo ampelographical descriptions. In addition, homonyms as well as varieties incorrectly cited as Albillo synonyms were identified. To ensure accurate variety identification, association among historical descriptions based on morphological descriptors and molecular fingerprints was required. Isozymes proved to be useful markers for varietal identification. However, only AFLP allowed distinction among closely related varieties as well as the analysis of intravarietal variation.M.T.C. was funded by a Ministerio de Educación y Cultura contract; I.R., J.A.C., and J.C. were funded by predoctoral fellowships from the European Union, Instituto Nacional de Investigaciones Agrarias y Agroalimentarias, and Agencia Española de Cooperación Internacional, respectively. This research was funded in part by projects INIA SC96-010, SC94-092, and CM 07B-0010-1997. Support for research activity at the Centro Nacional de Biotecnología is provided by a CSIC-INIA specific agreement.Peer reviewe

Fruit quality of Chenin Blanc grape influenced by different rootstocks during a production cycle in the second half.

This study aimed to evaluate the influence of different rootstocks on physico-chemical characteristics of the fruits of Chenin Blanc grapes produced in the second half of the year

Polarization of Lambda^0 hyperons in nucleus-nucleus collisions at high energies

The measurement of Lambda^0 hyperons polarization in nucleus-nucleus collisions is considered as one of possible tools to study the phase transition. Fixed target and collider experiments are discussed for the case of Lambda^0's production from Au-Au central collisions at \sqrt{s_{NN}} of several GeV.Comment: 15 pages, 6 figure

Regularity analysis of nocturnal oximetry recordings to assist in the diagnosis of sleep apnoea syndrome

The relationship between sleep apnoea–hypopnoea syndrome (SAHS) severity and the regularity of nocturnal oxygen saturation (SaO2) recordings was analysed. Three different methods were proposed to quantify regularity: approximate entropy (AEn), sample entropy (SEn) and kernel entropy (KEn). A total of 240 subjects suspected of suffering from SAHS took part in the study. They were randomly divided into a training set (96 subjects) and a test set (144 subjects) for the adjustment and assessment of the proposed methods, respectively. According to the measurements provided by AEn, SEn and KEn, higher irregularity of oximetry signals is associated with SAHS-positive patients. Receiver operating characteristic (ROC) and Pearson correlation analyses showed that KEn was the most reliable predictor of SAHS. It provided an area under the ROC curve of 0.91 in two-class classification of subjects as SAHS-negative or SAHS-positive. Moreover, KEn measurements from oximetry data exhibited a linear dependence on the apnoea–hypopnoea index, as shown by a correlation coefficient of 0.87. Therefore, these measurements could be used for the development of simplified diagnostic techniques in order to reduce the demand for polysomnographies. Furthermore, KEn represents a convincing alternative to AEn and SEn for the diagnostic analysis of noisy biomedical signals