Between-satellite ambiguity resolution based on preliminary GNSS orbit and clock information using a globally applied ambiguity clustering strategy.

The use of undifferenced (UD) processing schemes of GNSS measurements is becoming more and more popular for the generation of global network solutions (GNSS orbits and clock products) within the GNSS community. As opposed to classical processing schemes, which are based on a two-step approach where the orbits (generally, the contributions to the observation geometry) are estimated in a double-difference (DD) scheme while leaving the estimation of the corresponding clock information (and other linear terms) to a second, independent UD procedure where the orbits are introduced as known, the newer designs combine both parts into a single, compact processing scheme. Although this offers a higher flexibility, some challenges arise from the handling of the many parameters, as well as from the implementation of robust ambiguity resolution (AR) strategies. The latter could lead to a prohibitive computational time for a growing size of the network due to the large amount of ambiguity parameters. To overcome that issue, we propose a new UD-AR strategy that adapts the DD-AR approach. This is accomplished by carefully inspecting the real-valued ambiguities in a stand-alone step, where the DD-AR information is explicitly considered through the use of ambiguity clusters. As a result, the preliminary satellite orbits and clock corrections are modified to become consistent with the integer-cycle property of the carrier phase ambiguities, allowing to resolve them as integer numbers in a computationally inexpensive station-wise parallelization. This strategy is introduced and explained in detail. Moreover, it is shown that the GPS and Galileo solutions generated by this procedure are at a competitive level compared to classical DD-based solutions

Ontogenetic expression of thyroid hormone signaling genes: An in vitro and in vivo species comparison.

Thyroid hormone (TH) is essential for brain development. While disruption of TH signaling by environmental chemicals has been discussed as a mechanism of developmental neurotoxicity (DNT) for more than a decade, there remains a paucity of information linking specific TH disrupting chemicals to adverse neurodevelopmental outcomes. This data gap reflects, in part, the fact that the molecular machinery of TH signaling is complex and varies according to cell type and developmental time. Thus, establishing a baseline of the ontogenetic profile of expression of TH signaling molecules in relevant cell types is critical for developing in vitro and alternative systems-based models for screening TH disrupting chemicals for DNT. Here, we characterize the transcriptomic profile of molecules critical to TH signaling across three species-human, rat, and zebrafish-in vitro and in vivo across different stages of neurodevelopment. Our data indicate that while cultured human and rat neural progenitor cells, primary cultures of rat cortical cells, and larval zebrafish all express a fairly comprehensive transcriptome of TH signaling molecules, the spatiotemporal expression profiles as well as the responses to TH vary across species and developmental stages. The data presented here provides a roadmap for identifying appropriate in vitro and in simpler systems-based models for mechanistic studies and screening of chemicals that alter neurodevelopment via interference with TH action

Real time- and control software for the new orbit measurement system for the CERN SPS

The 240 channel SPS Orbit acquisition system is implemented on a PowerPC under the LynxOS operating system, making use of multi threaded real-time capabilities. The acquired data is transferred efficiently by DMA via the PCI bus into the main memory. System configuration aspects were implemented in a Broker architecture, where individual threads communicate with an Oracle database and the acquisition systems. This Broker hides the implementation details of the front-end systems. A versatile configuration client is provided in Java, to provide both local graphical user interfaces and remote WWW access using a dedicated gateway to the SL equipment layer. The timing diagnostics of the acquisition system are provided in a LabView application integrating oscilloscope control and channel multiplex control. This paper describes in detail the technical solutions implemented and reports on the arguments, which have led to particular choices

Solid phase modular synthesis of polymacromer brushes and their isolation as molecular products by photocleavage from the substrate

We report a novel solid phase method for the sequential coupling of heterobifunctional macromonomers to form a new class of polymeric materials that we refer to as polymacromers. Starting from an azide functional substrate, α-azido, ώ-protected-alkyne macromonomers are added step-by step by thermally initiated click reactions. After each addition step, the terminal alkyne group is deprotected to allow addition of another macromonomer. The use of highly chemoselective click chemistry for the coupling reactions allows virtually any macromonomer to be employed, regardless of its chemical nature. The polymacromers may be left as polymer brushes on the substrate, or can be isolated as molecular products by photocleavage of an ortho-nitrobenzyloxycarbonyl (NBOC) linkage incorporated at the substrate interface. The method is illustrated by forming homopolymacromers by sequential coupling of poly(tert-butyl acrylate) macromonomers. The results of characterization of the polymacromer bushes by ellipsometry, contact angle analysis and x-ray photoelectron spectroscopy, and direct measurements of the molecular weights of isolated products by gel permeation chromatography demonstrate that polymacromers can be prepared with a coupling efficiency approaching 100%

Impact of exposure of methicillin-resistant Staphylococcus aureus to polyhexanide in vitro and in vivo.

Staphylococcus aureus (MRSA) resistant to decolonization agents such as mupirocin and chlorhexidine increase the need to develop alternative decolonization molecules. The absence of reported adverse reactions and bacterial resistance to polyhexanide makes it an excellent choice as topical antiseptic. In the present study we evaluated the in vitro and in vivo capacity to generate strains with reduced polyhexanide susceptibility and cross-resistance with chlorhexidine and/or antibiotics currently used in clinic. Here we report the in vitro emergence of reduced-susceptibility to polyhexanide by prolonged-stepwise exposure to low concentrations in broth culture. Reduced susceptibility to polyhexanide was associated with genomic changes in the mprF and purR genes, and with concomitant decreased susceptibility to daptomycin and other cell-wall active antibiotics. However, the in vitro emergence of reduced-susceptibility to polyhexanide did not result in cross-resistance to chlorhexidine antiseptic. During in vivo polyhexanide clinical decolonization treatment, neither polyhexanide reduced-susceptibility nor chlorhexidine cross-resistance were observed. Together, these observations suggest that polyhexanide could be used safely for decolonisation of carriers of chlorhexidine-resistant S. aureus strains but highlight the need for careful use of polyhexanide at low antiseptic concentrations

Tropospheric Products from High-Level GNSS Processing in Latin America

ARTÍCULO PUBLICADO EN REVISTA EXTERNA. The present geodetic reference frame in Latin America and the Caribbean is given by a network of about 400 continuously operating GNSS stations. These stations are routinely processed by ten Analysis Centres following the guidelines and standards set up by the International Earth Rotation and Reference Systems Service (IERS) and International GNSS Service (IGS). The Analysis Centres estimate daily and weekly station positions and station zenith tropospheric path delays (ZTD) with an hourly sampling rate. This contribution presents some attempts aiming at combining the individual ZTD estimations to generate consistent troposphere solutions over the entire region and to provide reliable time series of troposphere parameters, to be used as a reference. The study covers ZTD and IWV series for a time-span of 5 years (2014–2018). In addition to the combination of the individual solutions, some advances based on the precise point positioning technique using BNC software (BKG NTRIP Client) and Bernese GNSS Software V.5.2 are presented. Results are validated using the IGS ZTD products and radiosonde IWV data. The agreement was evaluated in terms of mean bias and rms of the ZTD differences w.r.t IGS products (mean bias 1.5 mm and mean rms 6.8 mm) and w.r.t ZTD from radiosonde data (mean bias 2 mm and mean rms 7.5 mm). IWV differences w.r.t radiosonde IWV data (mean bias 0.41 kg/m2 and mean rms 3.5 kg/m2).Sitio de la revista: https://link.springer.com/chapter/10.1007/1345_2020_12

Steering Away from Current Amoxicillin Dose Reductions in Hospitalized Patients with Impaired Kidney Function to Avoid Subtherapeutic Drug Exposure.

Current dose reductions recommended for amoxicillin in patients with impaired kidney function could lead to suboptimal treatments. In a prospective, observational study in hospitalized adults with varying kidney function treated with an IV or oral dose of amoxicillin, amoxicillin concentrations were measured in 1-2 samples on the second day of treatment. Pharmacometric modelling and simulations were performed to evaluate the probability of target attainment (PTA) for 40% of the time above MIC following standard (1000 mg q6h), reduced or increased IV dosing strategies. A total of 210 amoxicillin samples was collected from 155 patients with kidney function based on a CKD-EPI of between 12 and 165 mL/min/1.73 m2. Amoxicillin clearance could be well predicted with body weight and CKD-EPI. Recommended dose adjustments resulted in a clinically relevant reduction in the PTA for the nonspecies-related PK/PD breakpoint MIC of 8 mg/L (92%, 62% and 38% with a CKD-EPI of 10, 20 and 30 mL/min/1.73 m2, respectively, versus 100% for the standard dose). For MICs ≤ 2 mg/L, PTA > 90% was reached in these patients following both reduced and standard dose regimens. Our study showed that for amoxicillin, recommended dose reductions with impaired kidney function could lead to subtherapeutic amoxicillin concentrations in hospitalized patients, especially when targeting less susceptible pathogens