Cigarette smoking is associated with amplified age-related volume loss in subcortical brain regions

BACKGROUND: Magnetic resonance imaging studies of cigarette smoking-related effects on human brain structure have primarily employed voxel-based morphometry, and the most consistently reported finding was smaller volumes or lower density in anterior frontal regions and the insula. Much less is known about the effects of smoking on subcortical regions. We compared smokers and non-smokers on regional subcortical volumes, and predicted that smokers demonstrate greater age-related volume loss across subcortical regions than non-smokers. METHODS: Non-smokers (n=43) and smokers (n=40), 22-70 years of age, completed a 4T MRI study. Bilateral total subcortical lobar white matter (WM) and subcortical nuclei volumes were quantitated via FreeSurfer. In smokers, associations between smoking severity measures and subcortical volumes were examined. RESULTS: Smokers demonstrated greater age-related volume loss than non-smokers in the bilateral subcortical lobar WM, thalamus, and cerebellar cortex, as well as in the corpus callosum and subdivisions. In smokers, higher pack-years were associated with smaller volumes of the bilateral amygdala, nucleus accumbens, total corpus callosum and subcortical WM. CONCLUSIONS: Results provide novel evidence that chronic smoking in adults is associated with accelerated age-related volume loss in subcortical WM and GM nuclei. Greater cigarette quantity/exposure was related to smaller volumes in regions that also showed greater age-related volume loss in smokers. Findings suggest smoking adversely affected the structural integrity of subcortical brain regions with increasing age and exposure. The greater age-related volume loss in smokers may have implications for cortical-subcortical structural and/or functional connectivity, and response to available smoking cessation interventions

Report of the panel on earth structure and dynamics, section 6

The panel identified problems related to the dynamics of the core and mantle that should be addressed by NASA programs. They include investigating the geodynamo based on observations of the Earth's magnetic field, determining the rheology of the mantle from geodetic observations of post-glacial vertical motions and changes in the gravity field, and determining the coupling between plate motions and mantle flow from geodetic observations of plate deformation. Also emphasized is the importance of support for interdisciplinary research to combine various data sets with models which couple rheology, structure and dynamics

Calculation of Turbulent Subsonic Diffuser Flows Using the NPARC Navier-Stokes Code

Axisymmetric subsonic diffuser flows were calculated with the NPARC Navier-Stokes code in order to determine the effects various code features have on the flow solutions. The code features examined in this work were turbulence models and boundary conditions. Four turbulence models available in NPARC were used: the Baldwin-Lomax algebraic model, the Baldwin-Barth one-equation model, and the Chien kappa-epsilon and Wilcox kappa-omega two-equation models. The three boundary conditions examined were the free boundary, the mass flux boundary and the subsonic outflow with variable static pressure. In addition to boundary condition type, the geometry downstream of the diffuser was varied to see if upstream influences were present. The NPARC results are compared with experimental data and recommendations are given for using NPARC to compute similar flows

Ocean Chlorophyll Studies from a U-2 Aircraft Platform

Chlorophyll gradient maps of large ocean areas were generated from U-2 ocean color scanner data obtained over test sites in the Pacific and Atlantic Oceans. The delineation of oceanic features using the upward radiant intensity relies on an analysis method which presupposes that radiation backscattered from the atmosphere and ocean surface can be properly modeled using a measurement made at 778 nm. An estimation of the chlorophyll concentration was performed by properly ratioing radiances measured at 472 nm and 548 nm after removing the atmospheric effects. The correlation between the remotely sensed data and in-situ surface chlorophyll measurements was validated in two sets of data. The results show that the correlation between the in-situ measured chlorophyll and the derived quantity is a negative exponential function and the correlation coefficient was calculated to be -0.965

Yolk sac erythromyeloid progenitors expressing gain of function PTPN11 have functional features of JMML but are not sufficient to cause disease in mice

Background: Accumulating evidence suggests the origin of juvenile myelomonocytic leukemia (JMML) is closely associated with fetal development. Nevertheless, the contribution of embryonic progenitors to JMML pathogenesis remains unexplored. We hypothesized that expression of JMML-initiating PTPN11 mutations in HSC-independent yolk sac erythromyeloid progenitors (YS EMPs) would result in a mouse model of pediatric myeloproliferative neoplasm (MPN). Results: E9.5 YS EMPs from VavCre+;PTPN11D61Y embryos demonstrated growth hypersensitivity to granulocyte-macrophage colony-stimulating factor (GM-CSF) and hyperactive RAS-ERK signaling. Mutant EMPs engrafted the spleens of neonatal recipients, but did not cause disease. To assess MPN development during unperturbed hematopoiesis we generated CSF1R-MCM+;PTPN11E76K;ROSAYFP mice in which oncogene expression was restricted to EMPs. Yellow fluorescent protein-positive progeny of mutant EMPs persisted in tissues one year after birth and demonstrated hyperactive RAS-ERK signaling. Nevertheless, these mice had normal survival and did not demonstrate features of MPN. Conclusions: YS EMPs expressing mutant PTPN11 demonstrate functional and molecular features of JMML but do not cause disease following transplantation nor following unperturbed development

Determination of DiazaCon in Quail Feed and Quail Serum by Ion Pair Reversed-Phase Chromatography

Liquid chromatographic (LC) methods were developed for quantitating the potential avian contraceptive DiazaCon in quail feed and serum. DiazaCon was extracted from ground quail feed with basic n-butyl chloride. The n-butyl chloride extract was evaporated to dryness. The DiazaCon residues were dissolved in an aqueous methanolic ion pairing solution and quantitated by LC at 206 nm. Avian sera was combined with an equal volume of a pH 4 aqueous solution of ion pairing reagent and filtered to remove interfering proteins. DiazaCon was quantitated by LC. Mean recoveries for 500 and 2000 ppm fortified feed were 89.1 and 91.0%, respectively. The mean recovery for sera fortified at 5 levels ranging from 35 to 2000 ppm was 84.9%. Method limits of detection were approximately 14 and 13 ppm for feed and sera, respectively

Finely Grinding Cereal Grains in Pelleted Diets Offers Little Improvement in Nursery Pig Growth Performance

Five experiments were conducted to determine the effects of corn particle size and diet form on nursery pig performance and feed preference. In Exp. 1, 192 nursery pigs (PIC 327 × 1050; initially 14.7 lb and 26 d of age) were used in a 35-d experiment. Pens of pigs were balanced by BW and allotted to 1 of 4 treatments with 6 pigs per pen and 8 pens per treatment. The same corn and soybean meal-based diet formulation was used for all treatments. The 2 × 2 factorial consisted of the main effects of corn particle size (400 vs. 700 μm) and diet form (mash vs. pelleted). Pigs fed mash diets had improved overall ADG and greater ADFI during all periods (P \u3c 0.05) and particle size did not impact (P \u3e 0.10) performance. In Exp. 2, a study utilized 96 pigs to evaluate feed preference of pigs consuming mash diets with either 400 or 700 μm corn. Pigs overwhelmingly (P \u3c 0.05) preferred to consume 700 μm corn compared to 400 μm corn (79.3 vs. 20.7%). In Exp. 3, 224 nursery pigs (PIC 327 × 1050; initially 24.1 lb and 40 d of age) were used in a 10-d experiment to determine the effects of corn particle size in pelleted diets on nursery pig performance. Experimental treatments were formed by grinding corn to 1 of 4 different particle sizes (250, 400, 550, or 700 μm). Particle size tended to affect (P \u3c 0.10) ADG in a quadratic manner, but did not impact (P \u3e 0.10) ADFI or F/G. Pigs fed pelleted diets from either 250 or 700 μm corn had poorer ADG than the intermediate treatments. Exp. 4 utilized 91 pigs to evaluate the preference of pigs consuming pelleted diets with either 250 or 700 μm corn from Exp. 3. Even in pelleted form, pigs preferred (P \u3c 0.05) to consume diets manufactured with the coarser particle size corn (58.2 vs. 41.8%). In Exp. 5, 180 nursery pigs (PIC 327 × 1050; initially 15.8 lb and 36 d of age) were used in a 35-d experiment to determine the effects of corn particle size and pelleting on nursery pig growth performance. The 2 × 2 factorial consisted of 2 corn particle sizes (500 μm vs. 750 μm) and two diet forms (mash vs. pelleted). Overall, reducing particle size from 750 to 500 μm did not affect growth performance (P \u3e 0.10). Pelleting reduced (P \u3c 0.05) feed intake, but did not affect ADG or F/G (P \u3e 0.10). These studies suggest that there is little value to be gained by grinding corn to less than 700 microns if fed in pelleted form. Furthermore, our data suggest that, regardless if fed as mash or pellets, pigs prefer to consume diets manufactured with coarser ground corn if given the choice