Effect of high pressure processing on carotenoid and phenolic compounds, antioxidant capacity, and microbial counts of bee-pollen paste and bee-pollen-based beverage

[EN] The optimal high pressure processing treatments (200 400 MPa, 5 15 min) of a pasty matrix of bee-pollen mixed with peptone water (1.5 g/mL) and bee-pollen added to a pineapple juice-based beverage matrix (0 10% (w/v)) were studied in order to guarantee food safety and maximum retention of bioactive compounds. Salmonella and yeasts were used as target microorganisms, while total carotenoid content (TCC), total phenolic content (TPC), and antioxidant capacity (FRAP) were studied from the food quality point of view. For the pasty matrix of bee-pollen, the results showed a significant influence of pressure and time, increasing the levels of TPC, FRAP, and TCC, in comparison with a control sample. A treatment of 395 MPa for 15 min was found as the optimal. For the pineapple juice-based beverage matrix, the factors pressure and bee-pollen concentration increased the levels of TPC, FRAP and TCC. Optimal conditions were found at 315 MPa for 14.5 min with 8% (w/v) of bee-pollen. Industrial relevance: This investigation demonstrated the efficacy of the application of HPP on bee-pollen to inactivate microorganisms, both pathogenic and spoilage, up to 5-log 10 cycles. Additionally, a structural modification of the grain was achieved, with a consequent extractability of bioactive compounds and an increasing in the antioxidant activity, higher than 60% in comparison to fresh bee-pollen. The inclusion of bee-pollen in a fruit juice-based beverage matrix had a positive effect on the contribution of bioactive compounds that the fruit juice itself does not contain, such as carotenoids, for which bee-pollen can be considered as a natural additive that enhances the product functional characteristics.The authors thank the Colombian Administrative Department of Science, Technology and Innovation (COLCIENCIAS) and the Research Direction of Universidad Nacional de Colombia for their support, project AGL 2013-48993-C2-2-R and FEDER funds for resourcesZuluaga, C.; Martínez, A.; Fernández, J.; López-Baldó, J.; Quiles Chuliá, MD.; Rodrigo, D. (2016). Effect of high pressure processing on carotenoid and phenolic compounds, antioxidant capacity, and microbial counts of bee-pollen paste and bee-pollen-based beverage. Innovative Food Science & Emerging Technologies. 37:10-17. https://doi.org/10.1016/j.ifset.2016.07.023S10173

Fracturas supracondíleas sobre prótesis total de rodilla

Presentamos 7 casos de fractura del extreme distal del fémur en pacientes con sustitución total de rodilla. El propósito del estudio consiste en valorar las distintas opciones terapéuticas y los resultados obtenidos a medio plazo. Los enfermos fueron tratados quirúrgicamente mediante un clavo intramedular u ortopédicamente con un sistema de tracción de Neufeld de una férula de tipo Q.T.B. El método de tratamiento se decidió en función del grado de desplazamiento de la fractura, tiempo transcurrido desde la misma y estado general del paciente. Todos alcanzaron un nivel de actividad similar al que poseían previo a la fractura.We present 7 cases of supracondylar fracture of the femur in patients with total knee arthroplaty. The purpose of this study was to asses the different options of treatment and their results at mid-term. Fractures were treated with an intramedular nail or with a Neufeld traction system followed by a Q.T.B. cast. The method of treatment depends on the grade of fracture displacement, period of time since the fracture and patient health. All o them reached similar level of activity like before the fracture

Loss of Bone Mineral Density Associated with Age in Male Rats Fed on Sunflower Oil Is Avoided by Virgin Olive Oil Intake or Coenzyme Q Supplementation

The role of dietary fat unsaturation and the supplementation of coenzyme Q have been evaluated in relation to bone health. Male Wistar rats were maintained for 6 or 24 months on two diets varying in the fat source, namely virgin olive oil, rich in monounsaturated fatty acids, or sunflower oil, rich in n-6 polyunsaturated fatty acids. Both dietary fats were supplemented or not with coenzyme Q10 (CoQ10). Bone mineral density (BMD) was evaluated in the femur. Serum levels of osteocalcin, osteopontin, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), adrenocorticotropin (ACTH) and parathyroid hormone (PTH), as well as urinary F2-isoprostanes were measured. Aged animals fed on virgin olive oil showed higher BMD than those fed on sunflower oil. In addition, CoQ10 prevented the age-related decline in BMD in animals fed on sunflower oil. Urinary F2-isoprostanes analysis showed that sunflower oil led to the highest oxidative status in old animals, which was avoided by supplementation with CoQ10. In conclusion, lifelong feeding on virgin olive oil or the supplementation of sunflower oil on CoQ10 prevented, at least in part mediated by a low oxidative stress status, the age-related decrease in BMD found in sunflower oil fed animals.This work was supported by the Spanish Ministry of Education and Science (AGL2008-01057) and the Autonomous Government of Andalusia (AGR832)

A telemedicine distributed system for cooperative medical diagnosis

Procceedings of: Eighteenth Annual Symposium on Computer Aplications in Medical Care, november 5-9, 1994, Washington, USA. Edited by Judy G. OzboltTelemedicine is changing the classicalform of health care delivery, dramatically increasing the number of new applications in which some type of distributed synchronous cooperation between health care professionals is required. This paper presents the design and development ofa telemedicine distributed system for cooperative medical diagnosis based on two new approaches: 1) a distributed layered architecture specially designed to add synchronous computer supported cooperative workfeatures either to new or existing medical applications; 2) the definition of a methodological procedure to design graphical user interfaces for telemedicine cooperative working scenarios. The cooperative work is supported by a collaborative toolkit that provides telepointing, window sharing, coordination and synchronization. Finally, we have implemented and installed the telemedicine system in clinical practice between two hospitals, providing teleconferencing facilities for cooperative decision support in haemodynamics studies. This specific implementation and a preliminary evaluation were accomplished under the Research Project FEST "Framework for European Services in Telemedicine" funded by the EU AIM Programme.This work is supported in part by EU - AIM FEST project no. A-201 1, and by grants CICYT TEMA TIC 92-1288-PB and TELEMEDICINA TIC 93-1279-E.Publicad

Diets based on virgin olive oil or fish oil but not on sunflower oil prevent age-related alvolar bone resorption by mitochondrial-related mechanisms

Background/Objectives: Aging enhances frequency of chronic diseases like cardiovascular diseases or periodontitis. Here we reproduced an age-dependent model of the periodontium, a fully physiological approach to periodontal conditions, to evaluate the impact of dietary fat type on gingival tissue of young (6 months old) and old (24 months old) rats.Methods/Findings: Animals were fed life-long on diets based on monounsaturated fatty acids (MUFA) as virgin olive oil, n-6 polyunsaturated fatty acids (n-6PUFA), as sunflower oil, or n-3PUFA, as fish oil. Age-related alveolar bone loss was higher in n-6PUFA fed rats, probably as a consequence of the ablation of the cell capacity to adapt to aging. Gene expression analysis suggests that MUFA or n-3PUFA allowed mitochondria to maintain an adequate turnover through induction of biogenesis, autophagy and the antioxidant systems, and avoiding mitochondrial electron transport system alterations.Conclusions: The main finding is that the enhanced alveolar bone loss associated to age may be targeted by an appropriate dietary treatment. The mechanisms involved in this phenomenon are related with an ablation of the cell capacity to adapt to aging. Thus, MUFA or n-3PUFA might allow mitochondrial maintaining turnover through biogenesis or autophagy. They might also be able to induce the corresponding antioxidant systems to counteract age-related oxidative stress, and do not inhibit mitochondrial electron transport chain. From the nutritional and clinical point of view, it is noteworthy that the potential treatments to attenuate alveolar bone loss (a feature of periodontal disease) associated to age could be similar to some of the proposed for the prevention and treatment of cardiovascular diseases, a group of pathologies recently associated with age-related periodontitis.This study was supported by I+D grants from the Spanish Ministry of Education and Science (AGL2008-01057) and the Autonomous Government of Andalusia (AGR832)

Low-Complexity TTCM Based Distributed Video Coding Architecture

Abstract. Distributed Video Coding (DVC) is a promising coding solution for some emerging applications, where the encoder complexity, power consumption or memory requirements are constraint the system resources. Current approaches to DVC focus on improving the performance of the WynerZiv coding by improving the quality of the reconstructed side information or by improving the quality of channel codes. Up to date, no attention has been paid to the problem of key frames coding where a low-encoding complexity scenario is also needed. This work focuses on key frames coding in its effect to the Wyner-Ziv frames decoding aiming to implement a very low-complexity Turbo Trellis Coded Modulation (TTCM) based DVC architecture. In this paper, we propose a new key frame coding scheme which has very low complexity and memory requirements for the TTCM based distributed video codec. Results show that the proposed intra frame codec for key frame coding outperforms the JPEG2000 and the Intra H.264 AVC codecs in terms of encoding-time and memory requirements, with better RD performance

Accuracy of digital planning in zygomatic implants

Background: Zygomatic implants have been described as a therapeutic alternative for patients with severe maxillary atrophy in order to avoid bone augmentation procedures. Taking that into account, in these treatments, the key factor is the position of the implant, the virtual surgical planning (VSP) is widespread among most clinicians before surgery on the patient. However, there are no studies which evaluate the clinical relevance of these VSP. The aim of this study is to determine whether digital planning on zygomatic implants has any influence on the implant dimensions and position, even when performing conventional surgery afterwards. Results: Fourteen zygomatic implants were placed in four patients. Pre-operative and post-operative helicoidal computed tomography were performed to each patient to allow the comparison between the digital planning and the final position of implants. Tridimensional deviation (TD), mesio-distal deviation (MDD), bucco-palatine deviation (BPD), and apico-coronal deviation (ACD) were evaluated as well as angular deviation (AD). Significative differences in apical TD were observed with a mean of 6.114 ± 4.28 mm (p < 0.05). Regarding implant position, only implants placed in the area of the first right molar reported significant differences (p < 0.05) for ACD. Also, implant length larger than 45 mm showed BPD significative differences (p < 0.05). Conclusions: Zygomatic implant surgery is a complex surgical procedure, and although VSP is a useful tool which helps the clinician determine the number and the length of zygomatic implants as well as its proper position, surgical experience is still mandatory.peer-reviewe

Industrial-Scale Decontamination Procedure Effects on the Content of Acaricides, Heavy Metals and Antioxidant Capacity of Beeswax

María Dolores Navarro-Hortal is a FPU fellow from the Spanish Ministry of Ciencia, Innovación y Universidades. We acknowledge Nutraceutical Translations for English language editing of this manuscript.Beeswax is useful for the beekeeping sector but also for the agro-food, pharmaceutical or cosmetics sectors. Frequently, this bee product is contaminated with pesticides reducing its utility and causing the decline in its market. This study aimed to prove the effectiveness of an industrial-scale decontamination method in removing acaricides from beeswax. Chlorfenvinphos and coumaphos decrease was higher than 90%, whereas tau fluvalinate decrease was only 30%. No changes were observed in the beeswax content of hydrocarbons and monoesters, whereas a decrease in the concentrations of Ca, Fe, Zn, Hg, Mn and P, and an increase in the concentrations of As and Si were found after the decontamination. Filtration reduced total phenolics, flavonoids and the antioxidant capacity of the lipophilic extract. These results demonstrate that the industrial method used was as effective as the method previously tested on a laboratory scale. The study also contributes to a better knowledge and characterization of beeswax, specially related to trace and ultra-trace elements and antioxidant capacity. Moreover, it offers the chance to further develop a method to effectively detect wax adulterations based on the chemical elements profile.The present study was partially funded by the “National Beekeeping Aid Program (Programa Nacional de Ayudas a la Apicultura)”, cofounded by the European Union and assigned to Spanish FEGA and FEAGA agencies (2016)

NLRP3 inflammasome suppression improves longevity and prevents cardiac aging in male mice

While NLRP3‐inflammasome has been implicated in cardiovascular diseases, its role in physiological cardiac aging is largely unknown. During aging, many alterations occur in the organism, which are associated with progressive impairment of metabolic pathways related to insulin resistance, autophagy dysfunction, and inflammation. Here, we investigated the molecular mechanisms through which NLRP3 inhibition may attenuate cardiac aging. Ablation of NLRP3‐inflammasome protected mice from age‐related increased insulin sensitivity, reduced IGF‐1 and leptin/adiponectin ratio levels, and reduced cardiac damage with protection of the prolongation of the agedependent PR interval, which is associated with atrial fibrillation by cardiovascular aging and reduced telomere shortening. Furthermore, old NLRP3 KO mice showed an inhibition of the PI3K/AKT/mTOR pathway and autophagy improvement, compared with old wild mice and preserved Nampt‐mediated NAD+ levels with increased SIRT1 protein expression. These findings suggest that suppression of NLRP3 prevented many age‐associated changes in the heart, preserved cardiac function of aged mice and increased lifespan.Andalusian regional government; Consejería de Salud de la Junta de Andalucia, Grant/ Award Number: PI‐0036‐2014; Ministerio de economía y competitividad, Grant/Award Number: SAF2017‐84494‐C2‐1‐