200 research outputs found
Turbulence attenuation by large neutrally buoyant particles
Turbulence modulation by inertial-range-size, neutrally-buoyant particles is
investigated experimentally in a von K\'arm\'an flow. Increasing the particle
volume fraction , maintaining constant impellers Reynolds
number attenuates the fluid turbulence. The inertial-range energy transfer rate
decreases as , suggesting that only particles
located on a surface affect the flow. Small-scale turbulent properties, such as
structure functions or acceleration distribution, are unchanged. Finally,
measurements hint at the existence of a transition between two different
regimes occurring when the average distance between large particles is of the
order of the thickness of their boundary layers.Comment: 7 pages, 4 figure
New pandemics: HIV and AIDS, HCV and chronic hepatitis, Influenza virus and flu
New pandemics are a serious threat to the health of the entire world. They are essentially of viral origin and spread at large speed. A meeting on this topic was held in Lyon, France, within the XIXth Jacques Cartier Symposia, a series of France-Québec meetings held every year. New findings on HIV and AIDS, on HCV and chronic hepatitis, and an update on influenza virus and flu were covered during this meeting on December 4 and 5, 2006. Aspects of viral structure, virus-host interactions, antiviral defenses, drugs and vaccinations, and epidemiological aspects were discussed for HIV and HCV. Old and recent data on the flu epidemics ended this meeting.The
meeting sponsors were the Centre Jacques Cartier, the Agence Nationale
de Recherches sur le SIDA et les hépatites (ANRS) France, the Ecole Normale
Supérieure en Sciences de Lyon, The Réseau SIDA et Maladies Infectieuses
from the Fond de la Recherche en Santé du Québec (FRSQ), The
Institut de Recherches Cliniques de Montréal (IRCM), Boehringer Ingelheim,
Sanofi Aventis, ViroChem Pharma and Merck Frosst. The authors
thank the speakers for their meeting abstracts and comments that helped
writing this review
Simultaneous 3D measurement of the translation and rotation of finite size particles and the flow field in a fully developed turbulent water flow
We report a novel experimental technique that measures simultaneously in
three dimensions the trajectories, the translation, and the rotation of finite
size inertial particles together with the turbulent flow. The flow field is
analyzed by tracking the temporal evolution of small fluorescent tracer
particles. The inertial particles consist of a super-absorbent polymer that
renders them index and density matched with water and thus invisible. The
particles are marked by inserting at various locations tracer particles into
the polymer. Translation and rotation, as well as the flow field around the
particle are recovered dynamically from the analysis of the marker and tracer
particle trajectories. We apply this technique to study the dynamics of
inertial particles much larger in size (Rp/{\eta} \approx 100) than the
Kolmogorov length scale {\eta} in a von K\'arm\'an swirling water flow
(R{\lambda} \approx 400). We show, using the mixed (particle/fluid) Eulerian
second order velocity structure function, that the interaction zone between the
particle and the flow develops in a spherical shell of width 2Rp around the
particle of radius Rp. This we interpret as an indication of a wake induced by
the particle. This measurement technique has many additional advantages that
will make it useful to address other problems such as particle collisions,
dynamics of non-spherical solid objects, or even of wet granular matter.Comment: 18 pages, 7 figures, submitted to "Measurement Science and
Technology" special issue on "Advances in 3D velocimetry
PDF model based on Langevin equation for polydispersed two-phase flows applied to a bluff-body gas-solid flow,
The aim of the paper is to discuss the main characteristics of a complete
theoretical and numerical model for turbulent polydispersed two-phase flows,
pointing out some specific issues. The theoretical details of the model have
already been presented [Minier and Peirano, Physics Reports, Vol. 352/1-3, 2001
]. Consequently, the present work is mainly focused on complementary aspects,
that are often overlooked and that require particular attention. In particular,
the following points are analysed : the necessity to add an extra term in the
equation for the velocity of the fluid seen in the case of twoway coupling, the
theoretical and numerical evaluations of particle averages and the fulfilment
of the particle mass-continuity constraint. The theoretical model is developed
within the PDF formalism. The important-physical choice of the state vector
variables is first discussed and the model is then expressed as a stochastic
differential equation (SDE) written in continuous time (Langevin equations) for
the velocity of the fluid seen. The interests and limitations of Langevin
equations, compared to the single-phase case, are reviewed. From the numerical
point of view, the model corresponds to an hybrid Eulerian/Lagrangian approach
where the fluid and particle phases are simulated by different methods.
Important aspects of the Monte Carlo particle/mesh numerical method are
emphasised. Finally, the complete model is validated and its performance is
assessed by simulating a bluff-body case with an important recirculation zone
and in which two-way coupling is noticeable.Comment: 23 pages, 10 figure
Convergent Chaos
Chaos is widely understood as being a consequence of sensitive dependence upon initial conditions. This is the result of an instability in phase space, which separates trajectories exponentially. Here, we demonstrate that this criterion should be refined. Despite their overall intrinsic instability, trajectories may be very strongly convergent in phase space over extremely long periods, as revealed by our investigation of a simple chaotic system (a realistic model for small bodies in a turbulent flow). We establish that this strong convergence is a multi-facetted phenomenon, in which the clustering is intense, widespread and balanced by lacunarity of other regions. Power laws, indicative of scale-free features, characterize the distribution of particles in the system. We use large-deviation and extreme-value statistics to explain the effect. Our results show that the interpretation of the 'butterfly effect' needs to be carefully qualified. We argue that the combination of mixing and clustering processes makes our specific model relevant to understanding the evolution of simple organisms. Lastly, this notion of convergent chaos, which implies the existence of conditions for which uncertainties are unexpectedly small, may also be relevant to the valuation of insurance and futures contracts
Inhibition of HIV-1 expression and replication by SOFA-HDV ribozymes against Tat and Rev mRNA sequences
Dynamics and statistics of heavy particles in turbulent flows
We present the results of Direct Numerical Simulations (DNS) of turbulent
flows seeded with millions of passive inertial particles. The maximum Taylor's
Reynolds number is around 200. We consider particles much heavier than the
carrier flow in the limit when the Stokes drag force dominates their dynamical
evolution. We discuss both the transient and the stationary regimes. In the
transient regime, we study the growt of inhomogeneities in the particle spatial
distribution driven by the preferential concentration out of intense vortex
filaments. In the stationary regime, we study the acceleration fluctuations as
a function of the Stokes number in the range [0.16:3.3]. We also compare our
results with those of pure fluid tracers (St=0) and we find a critical behavior
of inertia for small Stokes values. Starting from the pure monodisperse
statistics we also characterize polydisperse suspensions with a given mean
Stokes.Comment: 13 pages, 10 figures, 2 table
An Euler Solver Based on Locally Adaptive Discrete Velocities
A new discrete-velocity model is presented to solve the three-dimensional
Euler equations. The velocities in the model are of an adaptive nature---both
the origin of the discrete-velocity space and the magnitudes of the
discrete-velocities are dependent on the local flow--- and are used in a finite
volume context. The numerical implementation of the model follows the
near-equilibrium flow method of Nadiga and Pullin [1] and results in a scheme
which is second order in space (in the smooth regions and between first and
second order at discontinuities) and second order in time. (The
three-dimensional code is included.) For one choice of the scaling between the
magnitude of the discrete-velocities and the local internal energy of the flow,
the method reduces to a flux-splitting scheme based on characteristics. As a
preliminary exercise, the result of the Sod shock-tube simulation is compared
to the exact solution.Comment: 17 pages including 2 figures and CMFortran code listing. All in one
postscript file (adv.ps) compressed and uuencoded (adv.uu). Name mail file
`adv.uu'. Edit so that `#!/bin/csh -f' is the first line of adv.uu On a unix
machine say `csh adv.uu'. On a non-unix machine: uudecode adv.uu; uncompress
adv.tar.Z; tar -xvf adv.ta
Characterization of the TRBP domain required for Dicer interaction and function in RNA interference
<p>Abstract</p> <p>Background</p> <p>Dicer, Ago2 and TRBP are the minimum components of the human RNA-induced silencing complex (RISC). While Dicer and Ago2 are RNases, TRBP is the double-stranded RNA binding protein (dsRBP) that loads small interfering RNA into the RISC. TRBP binds directly to Dicer through its C-terminal domain.</p> <p>Results</p> <p>We show that the TRBP binding site in Dicer is a 165 amino acid (aa) region located between the ATPase and the helicase domains. The binding site in TRBP is a 69 aa domain, called C4, located at the C-terminal end of TRBP. The TRBP1 and TRBP2 isoforms, but not TRBPs lacking the C4 site (TRBPsΔC4), co-immunoprecipitated with Dicer. The C4 domain is therefore necessary to bind Dicer, irrespective of the presence of RNA. Immunofluorescence shows that while full-length TRBPs colocalize with Dicer, TRBPsΔC4 do not. <it>tarbp2</it><sup>-/- </sup>cells, which do not express TRBP, do not support RNA interference (RNAi) mediated by short hairpin or micro RNAs against EGFP. Both TRBPs, but not TRBPsΔC4, were able to rescue RNAi function. In human cells with low RNAi activity, addition of TRBP1 or 2, but not TRBPsΔC4, rescued RNAi function.</p> <p>Conclusion</p> <p>The mapping of the interaction sites between TRBP and Dicer show unique domains that are required for their binding. Since TRBPsΔC4 do not interact or colocalize with Dicer, we suggest that TRBP and Dicer, both dsRBPs, do not interact through bound dsRNA. TRBPs, but not TRBPsΔC4, rescue RNAi activity in RNAi-compromised cells, indicating that the binding of Dicer to TRBP is critical for RNAi function.</p
Automatic Assessment of Speech Capability Loss in Disordered Speech
International audienceIn this article, we report on the use of an automatic technique to assess pronunciation in the context of several types of speech disorders. Even if such tools already exist, they are more widely used in a different context, namely, Computer-Assisted Language Learning, in which the objective is to assess nonnative pronunciation by detecting learners' mispronunciations at segmental and/or suprasegmental levels. In our work, we sought to determine if the Goodness of Pronunciation (GOP) algorithm, which aims to detect phone-level mispronunciations by means of automatic speech recognition, could also detect segmental deviances in disordered speech. Our main experiment is an analysis of speech from people with unilateral facial palsy. This pathology may impact the realization of certain phonemes such as bilabial plosives and sibilants. Speech read by 32 speakers at four different clinical severity grades was automatically aligned and GOP scores were computed for each phone realization. The highest scores, which indicate large dissimilarities with standard phone realizations, were obtained for the most severely impaired speakers. The corresponding speech subset was manually transcribed at phone level; 8.3% of the phones differed from standard pronunciations extracted from our lexicon. The GOP technique allowed the detection of 70.2% of mispronunciations with an equal rate of about 30% of false rejections and false acceptances. Finally, to broaden the scope of the study, we explored the correlation between GOP values and speech comprehensibility scores on a second corpus, composed of sentences recorded by six people with speech impairments due to cancer surgery or neurological disorders. Strong correlations were achieved between GOP scores and subjective comprehensibility scores (about 0.7 absolute). Results from both experiments tend to validate the use of GOP to measure speech capability loss, a dimension that could be used as a complement to physiological measures in pathologies causing speech disorders
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