Информационные интеллектуальные системы и семантический веб

В учебном пособии рассматриваются основные составляющие технологии семантического веба: XML, пространство имен, универсальный идентификатор ресурсов URI, XML Schema, XSL, RDF, RDF Schema и OWL. Особое внимание уделяется использованию DTD и XML Schema, а также модели DOM XML. Материал проиллюстрирован наглядными практическими примерами, разделы включают лабораторные работы. Предназначено для студентов специальностей "Прикладная лингвистика", "Прикладная информатика" и других информационных и компьютерных направлений

Perfringolysin O-induced plasma membrane pores trigger actomyosin remodeling and endoplasmic reticulum redistribution

Clostridium perfringens produces an arsenal of toxins that act together to cause severe infections in humans and livestock animals. Perfringolysin O (PFO) is a cholesterol-dependent pore-forming toxin encoded in the chromosome of virtually all C. perfringens strains and acts in synergy with other toxins to determine the outcome of the infection. However, its individual contribution to the disease is poorly understood. Here, we intoxicated human epithelial and endothelial cells with purified PFO to evaluate the host cytoskeletal responses to PFO-induced damage. We found that, at sub-lytic concentrations, PFO induces a profound reorganization of the actomyosin cytoskeleton culminating into the assembly of well-defined cortical actomyosin structures at sites of plasma membrane (PM) remodeling. The assembly of such structures occurs concomitantly with the loss of the PM integrity and requires pore-formation, calcium influx, and myosin II activity. The recovery from the PM damage occurs simultaneously with the disassembly of cortical structures. PFO also targets the endoplasmic reticulum (ER) by inducing its disruption and vacuolation. ER-enriched vacuoles were detected at the cell cortex within the PFO-induced actomyosin structures. These cellular events suggest the targeting of the endothelium integrity at early stages of C. perfringens infection, in which secreted PFO is at sub-lytic concentrations.This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior in the framework of the project POCI-01-0145-FEDER-030863 (PTDC/BIA-CEL/30863/2017) and Norte-01-0145-FEDER-000012—Structured program on bioengineered therapies for infectious diseases and tissue regeneration, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). Publication Fees were supported by ICBAS, University of Porto. CB and FSM were supported by FCT fellowships (SFRH/BD/112217/2015, SFRH/BPD/94458/2013, respectively). CB was a Fulbright and FLAD fellow. SS received support from FCT Investigator program (COMPETE, POPH, and FCT)

On the origin and pathway of the saline inflow to the Nordic Seas: insights from models

The behaviours of three high-resolution ocean circulation models of the North Atlantic, differing chiefly in their description of the vertical coordinate, are investigated in order to elucidate the routes and mechanisms by which saline water masses of southern origin provide inflows to the Nordic Seas. An existing hypothesis is that Mediterranean Overflow Water (MOW) is carried polewards in an eastern boundary undercurrent, and provides a deep source for these inflows. This study, however, provides an alternative view that the inflows are derived from shallow sources, and are comprised of water masses of western origin, carried by branches of the North Atlantic Current (NAC), and also more saline Eastern North Atlantic Water (ENAW), transported northwards from the Bay of Biscay region via a ‘Shelf Edge Current’ (SEC) flowing around the continental margins. In two of the models, the MOW flows northwards, but reaches only as far as the Porcupine Bank (53°N). In third model, the MOW also invades the Rockall Trough (extending to 60°N). However, none of the models allows the MOW to flow northwards into the Nordic Seas. Instead, they all support the hypothesis of there being shallow pathways, and that the saline inflows to the Nordic Seas result from NAC-derived and ENAW water masses, which meet and partially mix in the Rockall Trough. Volume and salinity transports into the southern Rockall Trough via the SEC are, in the various models, between 25 and 100% of those imported by the NAC, and are also a similarly significant proportion (20–75%) of the transports into the Nordic Seas. Moreover, the highest salinities are carried northwards by the SEC (these being between 0.13 and 0.19 psu more saline at the southern entrance to the Trough than those in the NAC-derived waters). This reveals for the first time the importance of the SEC in carrying saline water masses through the RockallTrough and into the Nordic Seas. Furthermore, the high salinities found on density surfaces appropriate to the MOW in the Nordic Seas are shown to result from the wintertime mixing of the saline near-surface waters advected northwards by the SEC/NAC system. Throughout, we have attempted to demonstrate the extent to which the models agree or disagree with interpretations derived from observations, so that the study also contributes to an ongoing community effort to assess the realism of our current generation of ocean models

Технологические решения для строительства эксплуатационной наклонно-направленной скважины глубиной 3035 метров на нефтяном месторождении Томской области

Целью данной работы – проектирование эксплуатационной скважины на нефтяном месторождении Томской области. В процессе работы был разработан проект на строительство эксплуатационной нефтяной скважины Урманского месторождении Томской области глубиной 3035 метров. В работе рассмотрен вопрос анализ возможности использования различных сочетаний материалов для рабочей пары ВЗД.The purpose of this work is the design of a production well in an oil field in the Tomsk Region. In the course of work, a project was developed for the construction of an operational oil well at the Urmanskoye field in the Tomsk Region, 3035 meters deep. The paper discusses the possibility of using different combinations of materials for a working pair of PDMs

Autoantibodies to aS1-Casein Are Induced by Breast-Feeding

Background: The generation of antibodies is impaired in newborns due to an immature immune system and reduced exposure to pathogens due to maternally derived antibodies and placental functions. During nursing, the immune system of newborns is challenged with multiple milk-derived proteins. Amongst them, caseins are the main constituent. In particular, human aS1-casein (CSN1S1) was recently shown to possess immunomodulatory properties. We were thus interested to determine if auto-antibodies to CSN1S1 are induced by breast-feeding and may be sustained into adulthood. Methods: 62 sera of healthy adult individuals who were (n = 37) or were not (n = 25) breast-fed against human CSN1S1 were investigated by a new SD (surface display)-ELISA. For cross-checking, these sera were tested for anti Epstein-Barr virus (EBV) antibodies by a commercial ELISA. Results: IgG-antibodies were predominantly detected in individuals who had been nursed. At a cut-off value of 0.4, the SDELISA identified individuals with a history of having been breast-fed with a sensitivity of 80% and a specificity of 92%. Under these conditions, 35 out of 37 sera from healthy donors, who where breast-fed, reacted positively but only 5 sera of the 25 donors who were not breast-fed. The duration of breast-feeding was of no consequence to the antibody reaction as some healthy donors were only short term breast-fed (5 days minimum until 6 weeks maximum), but exhibited significant serum reaction against human CSN1S1 nonetheless. Conclusion: We postulate that human CSN1S1 is an autoantigen. The antigenicity is orally determined, caused by breastfeeding, and sustained into adulthood

Worms:Pernicious parasites or allies against allergies?

Host-parasite interactio

Analysis of a rapid sea ice retreat event in the Bellingshausen Sea

The winter advance of the sea ice edge in the Bellingshausen Sea is frequently interrupted by periods of rapid retreat lasting a few days. The frequency and duration of such events strongly controls the location of the late winter sea ice edge in this sector of the Antarctic. We examine the dynamics and thermodynamics of a retreat event that occurred in May 2001 using data from a drifting buoy array together with diagnostics from a kinematic/thermodynamic ice growth model and a high-resolution (11 km) regional coupled ocean-ice model. During the retreat event, the ice edge retreated by 250 km over 13 days in response to strong and persistent northerly winds associated with a quasi-stationary low-pressure system. Ice motion in the outer part of the pack was convergent and correlated strongly with local wind forcing. By contrast, in the region closer to the coast, ice motion was less well correlated with wind forcing. Model diagnostics indicate that ice thickening resulting from convergence in the outer pack was largely balanced by basal melting. In the outer pack, ice was in a state close to free drift while, closer to the coast, internal ice stresses became significant. The ocean-ice model simulated the characteristics of the retreat event realistically, giving us confidence in the ability of such models to reproduce ice conditions in this sector