Lizard thermoregulation revisited after two decades of global warming

CRUE-CSIC (Acuerdos Transformativos 2022)1.Although the effects of global warming on thermoregulation are usually explored using predictions of climate envelop modelling, such effects should best be analysed empirically, studying the same population with the same methods after a long enough period of temperature rise. 2. We used a 30-year long database about body temperatures (Tbs) of field-active Psammodromus algirus lizards inhabiting a well-conserved temperate open forest, and we focused on the summers of 1997 and 2017 to compare Tbs, environmental operative temperatures (Tes), their proximity to the selected thermal range (Tsel), and the selection of sunlit and shaded patches all along the day. From these data, we estimated the precision (standard deviation of Tbs), accuracy (average distance between Tbs and Tsel) and effectiveness (extent to which Tbs are closer to Tsel than Tes) of thermoregulation. 3. Of the highest 5% of all Tbs in the database, 95% were recorded in 2017, when the adjustment to Tsel was much better for Tbs selected in a laboratory thermogradient than for field Tbs (percentages of Tbs above Tsel of 2% and 52% respectively). 4. In 2017, especially after 12:00 h, the selection of shaded patches (87% of lizards in full shade vs. <1% in full sun) was more intense than in 1997, contributed more to overall thermoregulation, and produced a larger difference between Tes and Tbs. In spite of this, Tbs were lower—and closer to Tsel—in 1997 (when most shaded patches offered favourable thermal opportunities, with Tes within or below Tsel) than in 2017 (when only 33% of full shade Tes, and 8% of all Tes, were within or below Tsel). As a consequence, estimates of the accuracy and effectiveness of thermoregulation decreased over the 20-year period examined. 5. We conclude that given the low availability of Tes within or below Tsel, lizards cannot longer prevent the rise of their Tbs above Tsel, at least in hot summer days. Thus, the effects of global warming are already hindering the ability of lizards to buffer environmental change by behavioural means, even in temperate forests with a fine-grained mosaic of sun and shade patches.Depto. de Biodiversidad, Ecología y EvoluciónFac. de Ciencias BiológicasTRUEMinisterio de Ciencia e Innovación (MICINN)pu

Gamification as a tool for acquisition soft skills in the design field

Comunicació presentada a INTED2018, 12th International Technology, Education and Development Conference. (March 5-7 2018, Valencia, Spain).The current labour context is increasingly changing, driven by the continuous blooming of technological changes. So, the integration of recent graduates to the labour market, as new professionals, demand the acquisition of not only each field’s specific Hard Skills, but also generic, transversal Soft Skills which might facilitate the adaptation of these professionals to future contexts, while enabling them to develop their work career in a responsible way, as well as increasing their entrepreneurial spirit. These abilities are especially relevant in the field of design engineering, as the idiosyncrasy of the discipline leads their practitioners to offering solutions to diverse problems society and users have. So their professional development is inextricably linked to social changes and society evolution. In this way, in the “Sustainable Development Goal 4, Education 2030” (SDG4-Education 2030), regarding targets and commitments, the UNESCO defines the needs to emphasize the development of high-level cognitive and non-cognitive/transferable skills, such as problem solving, critical thinking, creativity, teamwork, communication skills or conflicts resolution, which can be applied across a wide range of occupational fields. Therefore, the acquisition of these Soft Skills by design professionals can ease their adaptation to a novel future scenario and guarantee a prolonged professional development over time. So, not only learners should be provided with opportunities to update their skills continuously through lifelong learning, but it is also necessary to give the chance for active professionals to adapt to new contexts in the future. This work presents an action proposal aimed to enhance the acquisition of Soft Skills by design professionals, in a playful way. So, a conceptual proposal of a methodology, based on the concept of Gamification, is suggested, where the degree of acquisition of some Soft Skills by the designer would be assessed. To do so, a platform where sharing and assessing evidences is to be developed. And the incentive for the participation of both new graduates and professionals lies in the detection of their professional weaknesses (from the evidences’ assessment) and the possibility of having a feedback consisting of professional recycling actions. The use of Smartphones becomes the vehicular platform for acquiring and assessing these transversal abilities, as these devices offer different technological possibilities for generating the designers’ own evidences, by means of actions such as voice recording, video recording, image capturing, text recognition, etc. Once generated, the evidences would be assessed by using a co-evaluation system, based on a peer review process, where the evaluation of colleagues’ work is presented as a part of a collaborative game and where the assessment process of peers’ work can contribute ideas for improving personal Soft Skills. Furthermore, all this may end up in the generation of a community with common interests. As a result, the suggested methodology can offer a useful tool for designers for continuously assessing the state of their Soft Skills, thus facilitating their progressive adaptation to the changes to their future professional contexts

Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.

Communication through cell-cell contacts and extracellular vesicles (EVs) enables immune cells to coordinate their responses against diverse types of pathogens. The function exerted by EVs in this context depends on the proteins and nucleic acids loaded into EVs, which elicit specific responses involved in the resolution of infection. Several mechanisms control protein and nucleic acid loading into EVs; in this regard, acetylation has been described as a mechanism of cellular retention during protein sorting to exosomes. HDAC6 is a deacetylase involved in the control of cytoskeleton trafficking, organelle polarity and cell migration, defense against Listeria monocytogenes (Lm) infection and other immune related functions. Here, we show that the protein content of dendritic cells (DCs) and their secreted EVs (DEVs) vary during Lm infection, is enriched in proteins related to antiviral functions compared to non-infected cells and depends on HDAC6 expression. Analyses of the post-translational modifications revealed an alteration of the acetylation and ubiquitination profiles upon Lm infection both in DC lysates and DEVs. Functionally, EVs derived from infected DCs upregulate anti-pathogenic genes (e.g. inflammatory cytokines) in recipient immature DCs, which translated into protection from subsequent infection with vaccinia virus. Interestingly, absence of Listeriolysin O in Lm prevents DEVs from inducing this anti-viral state. In summary, these data underscore a new mechanism of communication between bacteria-infected DC during infection as they alert neighboring, uninfected DCs to promote antiviral responses.This study was supported by grant PDI-2020-120412RB-I00, PDC2021-121797-I00, BIO2015-67580-P and PGC2018-097019-BI00 from the Spanish Ministry of Economy and Competitiveness (MINECO), grant S2017/BMD-3671-INFLAMUNE-CM from the Comunidad de Madrid, a grant from the Ramón Areces Foundation “Ciencias de la Vida y la Salud” (XIX Concurso-2018), “la Caixa” Banking Foundation (grants HR17-00016 and HR17-00247), BIOIMID (PIE13/041) and PRB3 (IPT17/0019 - ISCIII-SGEFI/ ERDF, ProteoRed) from Instituto de Salud Carlos III, CIBER Cardiovascular (CB16/11/00272), and Fondo de Investigación Sanitaria del Instituto de Salud Carlos III and co-funding by Fondo Europeo de Desarrollo Regional FEDER). IF-D is supported by a Fellowship from the Spanish Ministry of Science, Innovation, and Universities (FPU15/02539). DC-F is supported by a Fellowship from “la Caixa” Foundation (LCF/BQ/DR19/11740010). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX2020- 001041-S). Funding agencies did not intervene in the design of the studies, with no copyright over the study.S

DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]

Supplementary Figure 1. Isolated EVs present typical size and topology. Supplementary Figure 2. Protein profiling from total cell lysates and their derived EVs from WT and KO-HDAC6 BMDCs. Supplementary Figure 3. Enrichment in acetylated and ubiquitinated DC proteins upon Lm infection. Supplementary Figure 4. Ubiquitination in K-48 and K-63 state in T lymphoblast total cell lysates and their derived EVs. Supplementary Figure 5. Pore filtration methods restrain Lm and do not induce strong antipathogenic responses. Supplementary Figure 6. IFN-β is detected following Lm infection. Table S1. List of antibodies used for Western-blot and Flow Cytometry and the used dilution. Table S2. List of primers, with their corresponding sequence, used for qPCR. Table S3: Protein quantification in total cell lysates Table S4: IPA analysis of total cell lysates: canonical pathways and diseases and functions category Table S5: Protein quantification in EVs Table S6: IPA analysis of EVs: diseases and functions category Table S7: Ubiquitinated and acetylated peptides in total cell lysates and EVs Table S8: Enrichment analysis of ubiquitinated and acetylated proteinsPeer reviewe

TIC Y SISTEMAS INTELIGENTES como herramientas de soporte para el manejo, educación y prevención del trauma

La corporación ecuatoriana para el desarrollo de la investigación y la academia,. CEDIA, promueve la exploración y resultados de proyectos innovadores que vinculan a instituciones ecuatorianas. A inicios del año 2014, CEDIA conformó grupos de trabajo en varias áreas d einterés; una de ellas fue Telemedicina y Telesalud debido al gran impacto que tiene dentro de la investigación. Este grupo cuenta con la participación de seis universidades ecuatorianas: Universidad de Cuenca, Universidad Salesiana, Universidad Católica de Cuenca, Universidad del Azuay, Universidad Regional Autónoma de los Andes y Universidad Nacional de Chimborazo. El objetivo de este grupo de trabajo es fortalecer, impulsar y motivar actividades de investigación entre las instituciones miembros de CEDIA en bienestar d ela comunidad. En este libro se visualiza el trabajo realizado por el Grupo de Trabajo en temas de trauma utilizando múltiples formas de tecnología, desde aplicaciones móviles, e-learning, objetos de aprendizaje, hasta sistemas de recolección automatizada de datos; desarrollando programas que impactan en la atención de la salud en el área pre-hospitalaria, prevención de lesiones, registro de trauma, modalidades de educación y aprendizaje, mediante el uso de las TIC

Refugiado climático: hacia la garantía en el ejercicio efectivo de los derechos de las personas en movilidad humana

Los embates producidos por el cambio climático han demostrado que este fenómeno no solo es una realidad, sino que ha cambiado las condiciones de vida de millones de personas. Esto abre la puerta para que la civilización se movilice en busca de mejores condiciones para vivir y con ello se permita la libre movilidad o circulación de todos aquellos a quienes su Estado, no les ha podido garantizar la aplicación de sus derechos, y, por ende, una calidad de vida acorde a sus necesidades. El presente artículo tiene como objetivo dilucidar la necesidad de crear nuevas instituciones o estatus jurídicos específicos que protejan los derechos de las personas en movilidad humana por razones climáticas. Para la realización de este artículo se han empleado los métodos cualitativo, descriptivo y bibliográfico, que implicó revisión de material documental, doctrina y el ordenamiento jurídico nacional e internacional. Como resultado de esta investigación se encontró que existen vastas justificaciones que apuntan a la necesidad de que la comunidad internacional se pronuncie sobre el vacío legal que existe para las personas que han tenido que desplazarse forzosamente por razones climáticas. En tal razón, se puede concluir que es imperante reconocer el concepto de refugiado hacia aquellos desplazados por situaciones climáticas que se han quedado en la indefensión ante la vulneración de sus derechos. Este reconocimiento incluye aceptar que la adversidad climática es una de las razones para que las personas en movilidad humana accedan al estatus de refugiado climático y así brindarles una respuesta que les permita rehacer su vida en condiciones dignas y al amparo legal de otras jurisdicciones

DataSheet_1_Assessment of the interactions between cetaceans and fisheries at the south of the Cetacean Migration Corridor and neighboring waters (Western Mediterranean).docx

The Cetacean Migration Corridor is an important marine protected area for cetacean species in the Western Mediterranean, and part of its waters constitute the main fishing grounds for the Valencia region (East Spain) fishing fleet. Here we aim to assess the interactions between all active fisheries operating in the waters of the Valencia region and the cetacean species inhabiting this area. A total of 282 face-to-face interviews (51.37% of the total fleet) to fishers were held on 20 ports at the study area to gather information about cetacean by-catch and all types of interactions between fisheries and cetaceans. The interviewed bottom trawlers (n = 148 boats) reported a monthly by-catch rate (C) of 0.01 dolphins per vessel. Bottom trawling vessels operating at neritic zones only reported by-catch of common bottlenose dolphin (C = 0.009), while those fishing at oceanic zones by-caught striped dolphin (C = 0.006) and common bottlenose dolphin (C = 0.003). The interviewed artisanal fishers (n = 114 boats), the second most important fishery in the study area, only reported one dolphin by-catch event, but 90 of these boats communicated continued negative interactions with the common bottlenose dolphin, causing gear damage and catch take. The interviewed vessels gave an estimation of their annual economic loss produced by this interaction (mean ± SD: 2,998.10 ± 2095.02 € per boat). Other fisheries operating in the Valencia region were purse-seiners (n = 15 boats) and pelagic longlines, a fishery that has almost disappeared in the study area (n = 5 boats). The first one had the highest estimated dolphin by-catch rate (C= 0.04) and mainly interacted with common bottlenose dolphin, but also with striped dolphin. The information gathered from interviews was complimented with long-term stranding data (1990–2020). Despite the fact that only 7.80% of all recorded stranded cetaceans showed fisheries interaction signs, 26.72% of the common bottlenose dolphins recorded showed evidence of this interaction, thus confirming that it is the cetacean species most affected by fisheries in the area. Records of stranded striped dolphin (6.45%) also showed evidence of fisheries interaction. Based on both dolphin by-catch and gear damage, management plans are needed in the area to ensure cetacean conservation and also fisheries sustainability in waters inside and around marine protected areas at the Spain’s Mediterranean.</p

DataSheet_2_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.zip [Dataset]

Table S3: Protein quantification in total cell lysates Table S4: IPA analysis of total cell lysates: canonical pathways and diseases and functions category Table S5: Protein quantification in EVs Table S6: IPA analysis of EVs: diseases and functions category Table S7: Ubiquitinated and acetylated peptides in total cell lysates and EVs Table S8: Enrichment analysis of ubiquitinated and acetylated proteinsPeer reviewe

Modified Sequential Organ Failure Assessment Score vs. Early Warning Scores in Prehospital Care to Predict Major Adverse Cardiac Events in Acute Cardiovascular Disease

(1) Background: The Modified Sequential Organ Failure Assessment (mSOFA) is an Early Warning Score (EWS) that has proven to be useful in identifying patients at high risk of mortality in prehospital care. The main objective of this study was to evaluate the predictive validity of prehospital mSOFA in estimating 2- and 90-day mortality (all-cause) in patients with acute cardiovascular diseases (ACVD), and to compare this validity to that of four other widely-used EWS. (2) Methods: We conducted a prospective, observational, multicentric, ambulance-based study in adults with suspected ACVD who were transferred by ambulance to Emergency Departments (ED). The primary outcome was 2- and 90-day mortality (all-cause in- and out-hospital). The discriminative power of the predictive variable was assessed and evaluated by the area under the curve (AUC) of the receiver operating characteristic (ROC). (3) Results: A total of 1540 patients met the inclusion criteria. The 2- and 90-day mortality rates were 5.3% and 12.7%, respectively. The mSOFA showed the highest AUC of all the evaluated scores for both 2- and 90-day mortality, AUC = 0.943 (0.917–0.968) and AUC = 0.874 (0.847–0.902), respectively. (4) Conclusions: The mSOFA is a quick and easy-to-use EWS with an excellent ability to predict mortality at both 2 and 90 days in patients treated for ACVD, and has proved to be superior to the other EWS evaluated in this study