A CASE STUDY OF MATCH ANALYSIS FOR PRACTICAL TENNIS COACHING

The purpose of this study was to verify a new coaching method through match analysis with video as a case study. A woman collegiate tennis player was selected for this study. The goal for the player was to decrease the problem pattern and to increase the strength pattern in actual match. The coaching period was about 5 weeks. In coaching process, the video clips edited by Gamebreaker (Sportstec) was used. Each rally in pre- and postmatch was categorised to ‘Good’, ‘Poor’ or ‘Neutral’. The frequencies and the ratios of each category were compared between pre- and post-match. As a result, The ratio of ‘Good’ category showed significantly high and the ratio of ‘Poor’ category showed significantly low in post-match compared to pre-match. It was considered those differences between pre- and post-match were caused by the coaching with video

Upstream activation element of the PH03 gene encoding for thiamine-repressible acid phosphatase in Saccharomyces cerevisiae

AbstractThe PH03 gene of Saccharomyces cerevisiae encodes thiamine-repressible acid phosphatase and requires the positively acting regulatory protein TH12 for its expression. Deletion analysis of the 5′-flanking region of PH03 gene revealed that an activating region located at nucleotide position −234 to −215 relative to the translation initiation codon is required for the expression and sensitivity to thiamine. A chemically synthesized DNA fragment covering −234 to −215 showed a significant level of expression when inserted in front of the PH03 promoter lacking the activating region. Electrophoretic mobility shift assay demonstrated the presence of proteins that bound to the above DNA fragment in the nuclear extract from cells grown in thiamine-free medium. These findings suggested that this region between −234 and −215 acts as an upstream activation element of the PH03 gene that can interact with regulatory proteins

Japan's Defence: Today and Tomorrow

When the Pacific War was over, I was taking a training to become a commanding officer of the KORYLI (Seadragon), a five-man midget submarine, modified version of the two-man submarine which penetrated Pearl Harbor in 1941. Missions in these submarines were not suicidal, but very nearly so, because they were automatically forced to the surface after firing one torpedo out of two due to the loss of balance. They would float in front of the target for about twenty seconds, submerge to normal depth, fire the second torpedo and come up again. It was a "secret weapon" in the hands of the Japanese Imperial Navy before the end of the war. About 150 such submarines were ready to fight against the landing forces of the United States. I mention this to underline the difference between my feelings at that time, and my feelings now. I had no fear of death during the war. I was not afraid of fighting against a superior force for the sake of the nation. But I am much more afraid of death now. Although 1 have taught for a long time at the National Defence College (currently the National Institute for Defence Studies) about international security, my sentiments are very different from what they were in the prewar period. The difference in my feelings is also tied to the atomic bombing of Hiroshima. I was in the Submarine School at Otake, 40 kilometers away from Hiroshima when the bomb was dropped, and saw the flash of the explosion and the mushroom cloud, and felt the shockwave. Two days later, I walked through Hiroshima for about two hours. I was told that if I had been there for five hours I would have been fatally affected, but fortunately I was not exposed to the radiation long enough for that. When I came back to Kure, which was near my home port at that time, my group and I got very drunk, to try and expunge the nightmare we had seen. The drinking helped save my life, flushing out the radiation. I do not know whether to believe that or not, but in any case I am still here. Only someone who had gone through the same experience, I think, could understand the anxieties I had after the war, especially when I was about to Hisao Iwashima have my first child. Even my wife did not really understand. At any rate, these feelings make up a part of my mentality. Frankly speaking, the Japanese "nuclear allergy" is something that ought to persist. It is unacceptable to use atomic weapons for any reason, under any reason, under any conditions. At the same time, I have to acknowledge the effectiveness of nuclear weapons as a deterrent. This is the dilemma I have to confront in my own thinking

Instituto Técnico Dr. Miguel Pereira

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Optically tunable fiber grating transmission filters

We propose and demonstrate single- and multiple-passband fiber grating transmission filters that are remotely tunable by exploitation of the optical pump-induced thermal effects in Er Yb-codoped fiber sections. A repeatable, wavelength-independent induced phase shift of 0.1p mW is obtained without hysteresis and anisotropic effects. A transmission extinction ratio of .23 dB with a 3-mW change in pump power is achieved

Emoções vivenciadas pelo psicoterapeuta aprendiz na clínica psicanalítica com adolescentes: um estudo exploratório / Emotions experienced by apprentice psychotherapists in the psychoanalytic clinic with adolescents: an exploratory study

A relação transferencial estabelecida na díade paciente-psicoterapeuta pode propiciar o surgimento de diversos sentimentos em ambos ao longo do processo psicoterapêutico. Tendo em vista o interesse em investigar as emoções do psicoterapeuta aprendiz na clínica psicanalítica, o presente estudo tem por objetivo descrever as emoções vivenciadas pelos psicoterapeutas a partir de fatos clínicos detectados no atendimento a adolescentes em psicoterapia psicanalítica. Foi realizada uma pesquisa qualitativa por meio do método de construção de fatos clínicos psicanalíticos, a partir dos relatórios de sessões clínicas de atendimento psicoterápico a duas adolescentes. Foram analisadas as vivências emocionais relatadas por duas psicoterapeutas decorrentes de fatos ocorridos durante cada processo psicoterápico. Duas emoções se destacaram e foram analisadas a partir de alguns fundamentos da psicanálise. Os resultados indicam que as psicoterapeutas aprendizes passaram por algo que poderíamos nomear de “adolescência profissional”, que poderia dificultar ou impedir a sua função de psicoterapeutas, como também funcionar como mola propulsora na busca de aprimoramento pessoal e profissional

Novel TPP-riboswitch activators bypass metabolic enzyme dependency

Riboswitches are conserved regions within mRNA molecules that bind specific metabolites and regulate gene expression. TPP-riboswitches, which respond to thiamine pyrophosphate (TPP), are involved in the regulation of thiamine metabolism in numerous bacteria. As these regulatory RNAs are often modulating essential biosynthesis pathways they have become increasingly interesting as promising antibacterial targets. Here, we describe thiamine analogs containing a central 1,2,3-triazole group to induce repression of thiM-riboswitch dependent gene expression in different E. coli strains. Additionally, we show that compound activation is dependent on proteins involved in the metabolic pathways of thiamine uptake and synthesis. The most promising molecule, triazolethiamine (TT), shows concentration dependent reporter gene repression that is dependent on the presence of thiamine kinase ThiK, whereas the effect of pyrithiamine (PT), a known TPP-riboswitch modulator, is ThiK independent. We further show that this dependence can be bypassed by triazolethiamine-derivatives that bear phosphate-mimicking moieties. As triazolethiamine reveals superior activity compared to pyrithiamine, it represents a very promising starting point for developing novel antibacterial compounds that target TPP-riboswitches. Riboswitch-targeting compounds engage diverse endogenous mechanisms to attain in vivo activity. These findings are of importance for the understanding of compounds that require metabolic activation to achieve effective riboswitch modulation and they enable the design of novel compound generations that are independent of endogenous activation mechanism