Determinación de la prevalencia de brucelosis bovina en la hacienda "Rancho Shilla", provincia del Cañar.

El objetivo de este proyecto experimental fue determinar la prevalencia de brucelosis bovina en la hacienda “Rancho Shilla”, Provincia del Cañar. Para el cumplimiento del objetivo se empleó dos tipos de pruebas de laboratorio en el diagnóstico de los animales seropositivos a la enfermedad de brucelosis, donde se extrajeron muestras de sangre de la vena coccígea de los 40 animales con los que se trabajó, posteriormente se realizó la evaluación de las muestras de sangre en el laboratorio aplicando la prueba Rosa de Bengala (RB) obtener así los animales sospechosos, luego todos los animales sospechosos fueron evaluados mediante la prueba Elisa competitiva (EC) la cual confirmó la seropositividad de los animales para Brucella abortus; luego fueron caracterizados los animales en neonatos, jóvenes y adultos, para finalmente poder analizar los factores de riesgo predisponentes donde se incluyeron variables como el sexo, edad, etapa productiva, abortos, síntomas y vacunación. Para analizar los resultados se utilizó estadística descriptiva donde existieron 18 animales sospechosos al utilizar RB y 17 animales confirmados al usar EC; al categorizar a los animales se encontró que la enfermedad está presente en el 5.9% de neonatos, 29.4% de animales jóvenes y 64.7 % en adultos; los factores de riesgo la variable sexo, abortos y síntomas presentaron diferencias altamente significativas, mientras que los factores edad, etapa productiva y vacunas no presentaron diferencias significativas. Al concluir con los análisis se ha determinado que la prevalencia de B. bovina fue del 45 % con RB y del 42.5% con EC, teniendo una mayor prevalencia en animales adultos del sexo hembra en la Hacienda. Se recomienda la vacunación con Cepa 19 o RB51 para mantener una ganadería libre de brucelosis.The aim of this experimental study was to determine the prevalence of bovine brucellosis at the farm Rancho Shilla located in the province of Cañar. To achieve this goal, two types of tests were used for the diagnosis of seropositive animals. Blood samples were collected from the coccygeal veins in 40 animals of this study. Then they were analyzed at the laboratory using the Rose Bengal test (RBT) to detect the suspected cases. Afterwards, the suspected cases were tested using the competitive ELISA, which confirmed that the animals were seropositive for Brucella abortus. Then the animals were characterized as neonates and young and adult animals. Finally, the risk factors were analyzed, and the variables, such as sex, age, productive stage, abortions, symptoms. and vaccination were included. Descriptive statistics was used to analyze the results. A total of 18 suspected cases were confirmed using RBT, and a total of 17 suspected cases were confirmed using ELISA. When characterizing the animals, it was found that the disease is prevalent in neonates (5.9%), young animals (29.4%), and adults (64.7%). Regarding the risk factors, the variables, such as sex, abortions, and symptoms showed high significant differences, whereas age, productive stage, and vaccination did not show significant differences. To conclude, the prevalence of bovine brucellosis was 45% using RBT and 42% using ELISA. The prevalence was higher among female adults at the farm. Strain 19 or RB51 vaccines must be administered to have a brucellosis-free herd

Ditopic receptors containing urea groups for solvent extraction of Cu(II) salts

[Abstract] The ditopic receptor L3 [1-(2-((7-(4-(tert-butyl)benzyl)-1,4,7,10-tetraazacyclododecan-1-yl)methyl)phenyl)-3-(3-nitrophenyl)urea] containing a macrocyclic cyclen unit for Cu(II)-coordination and a urea moiety for anion binding was designed for recognition of metal salts. The X-ray structure of [CuL3(SO4)] shows that the sulfate anion is involved in cooperative binding via coordination to the metal ion and hydrogen-bonding to the urea unit. This behaviour is similar to that observed for the related receptor L1 [1-(2-((bis(pyridin-2-ylmethyl)amino)methyl)phenyl)-3-(3-nitrophenyl)urea], which forms a dimeric [CuL1(μ-SO4)]2 structure in the solid state. In contrast, the single crystal X-ray structure of [ZnL3(NO3)2] contains a 1 : 2 complex (metal : anion) where one anion coordinates to the metal and the other is hydrogen-bonded to the urea group. Spectrophotometric titrations performed for the [CuL3(OSMe2)]2+ complex indicate that this system is able to bind a wide range of anions with an affinity sequence: MeCO2− > Cl− > H2PO4− > Br− > NO2− > HSO4− > NO3−. Lipophilic analogues of L1 and L3 extract CuSO4 and CuCl2 from water into chloroform with high selectivity over the corresponding Co(II), Ni(II) and Zn(II) salts.Xunta de Galicia; EM 2012/088Xunta de Galicia; CN-2012/01

La incorporación de la voz del alumnado en el desarrollo de una estructura curricular modular. La experiencia del grado de educación social en la UPV-EHU

Con el objetivo de acercar las prácticas universitarias al discurso de democratización educativa, este artículo recoge la experiencia de la incorporación de la voz del alumnado en el desarrollo de la estructura modular del Grado de Educación Social de la UPV-EHU. Centrándonos en un análisis del efecto de la incorporación de la voz del alumnado en las estructuras organizativas, en diferentes contextos, en su propio aprendizaje y en el cambio de las prácticas docentes, hemos intentado desvelar el sentido otorgado a la participación del alumnado en la vida formativa y universitaria, detectar los claroscuros que se visualizan a su acción como sujeto y analizar el efecto producido en su aprendizaje.With the aim of bringing university practices to address educational democratization, this article describes the experiences of incorporating student voice in the development of the modular structure of Social Education degree from the UPV-EHU. Focusing on an analysis of the effect of the incorporation of student voice in organizational structures, in different contexts, in their own learning and changing teaching practices, we tried to reveal the meaning given to student participation in the life training and university detect shades that are displayed to its action as a subject and analyze the effect on their learning

Recognition of AMP, ADP and ATP through cooperative binding by Cu(II) and Zn(II) complexes containing urea and/or phenylboronic acid moieties

[Abstract] We report a series of Cu(II) and Zn(II) complexes with different ligands containing a dipicolyl unit functionalized with urea groups that may contain or not a phenylboronic acid function. These complexes were designed for the recognition of phosphorylated anions through coordination to themetal ion reinforced by hydrogen bonds involving the anion and NHgroups of urea. The complexes were isolated and several adducts with pyrophosphate were characterized using X-ray diffraction measurements. Coordination of one of the urea nitrogen atoms to themetal ion promoted the hydrolysis of the ligands containing 1,3-diphenylurea units, while ligands bearing 1-ethyl-3-phenylurea groups did not hydrolyze significantly at room temperature. Spectrophotometric titrations, combined with 1H and 31P NMR studies, were used in investigating the binding of phosphate, pyrophosphate (PPi), and nucleoside 50-polyphosphates (AMP, ADP, ATP, CMP, and UMP). The association constants determined in aqueous solution (pH 7.0, 0.1MMOPS) point to a stronger association with PPi, ADP, and ATP as compared with the anions containing a single phosphate unit. The [CuL4]2+ complex shows important selectivity for pyrophosphate (PPi) over ADP and ATP.Galicia. Consellería de Cultura, Educación e Ordenación Universitaria; EM 2012/08

Aprendizaje integrado de software para producción de buques

Una alta productividad en Ingeniería está asociada entre otras cosas a una gestión eficiente del flujo de las enormes cantidades de información y correspondientes tomas de decisiones consubstanciales a los entornos de diseño y producción. Ello exige que nuestros titulados estén formados para ser capaces de manejar esa información de modo integrado, a través de los diferentes estadios en que se genera y procesa. En el contexto de la enseñanza de la Ingeniería existen un buen número de cursos designados a desarrollar competencias específicas, como las requeridas en los currículos académicos, pero muy pocos en los cuales las competencias de integración sean el objetivo principal. En este artículo se documenta una asignatura que tiene esa orientación, con la intención de alimentar el debate sobre la formación, tan necesario en tiempos de fuerte contracción de la actividad económica como el que vivimos en la actualidad y que está promoviendo el COIN. La asignatura se denomina “Tecnologías de la Información Aplicadas a la Construcción Naval” y es impartida en la titulación de Ingeniero Naval y Oceánico, plan 2002, de la Universidad Politécnica de Madrid (UPM). La primera parte de la asignatura está dedicada a la planificación y gestión de proyectos; los estudiantes adquieren competencias en la definición, usando Ms-PROJECT, de la estructura de descomposición de tareas y la asignación de recursos así como en el seguimiento de proyectos, a través de una serie de ejemplos de complejidad creciente, finalizando con la construcción de un buque. La segunda parte está dedicada al uso de un gestor de bases de datos, Ms-ACCESS, y orientada a la gestión de la información relativa a Producción. Otra vez la estrategia pasa por trabajar sobre una serie de ejemplos de complejidad creciente y se finaliza con la administración de una base de datos de tuberías de un buque, para las cuales se establecen hitos de producción, recepción y montaje, lo que conecta está parte con la primera. Finalmente, la tercera parte de la asignatura está dedicada al trabajo con FORAN, con el cual se definen con detalle los elementos estructurales de todo el buque sobre el que se ha elaborado la planificación. En esta parte, el trabajo es cooperativo, dado que los estudiantes operan simultáneamente en el mismo modelo 3D. Las clases se realizan en un aula multimedia en la cual cada estudiante puede utilizar todas las aplicaciones de software tratadas. Se han realizado encuestas a los estudiantes para obtener una retroalimentación desde su experiencia así como para hacer una valoración de su satisfacción con el proceso de aprendizaje. Los resultados de esas encuestas son discutidos en el artículo

Cinderella Elements: Strategies to Increase the Stability of Group 1 Complexes by Tailoring Crown Macrocycles

[Abstract] The synthesis and structural characterization of six sodium complexes with bibracchial lariatethers containing aniline or benzimidazole side arms, and derived from 1,7 diaza-12-crown-4, 1,10-diaza-15-crown-5 or 4,13-diaza-18-crown-6, are reported. The X-ray structures of four of these compounds have been obtained. Additionally, the X-ray structures of a sodium macrobicyclic complex derived from 1,10-diaza-15-crown-5, and a potassium complex with a bibracchial lariat ether containing aniline side arms are also reported. Bonding distances as well as the stability constants in acetonitrile solution confirm that the coordination of the pendant arms provides an important contribution to the overall stability of the complexes, particularly when benzimidazole pendants are present rending more stable complexes, even more than cryptand complexes of the same size. Compared with the parent crown ethers, the stability increases when the side arms contain benzimidazole moieties but remains about the same order when aniline side arms are present.Xunta de Galicia; CN2012/01

Phenotypic Characterization of Encephalitis in the BRAINS of Badgers Naturally Infected with Canine Distemper Virus

[EN] Canine distemper virus (CDV) affects a huge diversity of domestic and wild carnivores, with increasing numbers of mortality events worldwide. The local cell-mediated immune response elicited against a natural infection is an important factor in determining the outcome of CDV infection. Therefore, the purposes of this study were to describe the local immune response within the central nervous systems (CNSs) of seven badgers naturally infected with CDV in Asturias (Atlantic Spain) and to determine the phenotype and distribution of microglial cells, T and B lymphocytes, and astrocytes in the foci of gliosis located in the thalamus and cerebellum using immunohistochemistry. The immunohistochemical assessment demonstrated the presence of Iba1-positive microglia and GFAP-positive astrocytes in the foci of gliosis, whereas T (CD3-negative) or B (CD20-negative) lymphocytes in those same lesions were absent. Our results also revealed that the badgers with natural CDV encephalitis presented lesions mostly located in the white matter of the thalamus and cerebellum, suggesting a CDV-specific tropism for the white matter of badger brains in those locations. The knowledge gained in the field of the immunopathogenesis of distemper disease affecting the CNSs of badgers could help to clarify CDV disease patterns in this speciesSIThis work was partially funded by the Principality of Asturias (PCTI 2021–2023, GRUPIN: IDI-2021-000102) and European Regional Development Fun

Variability of the lower limb symmetry index associated with the gait parameters in the overweight adult population with flatfoot: a case-control study

Background: Adult acquired flatfoot is characterized by a medial arch collapse during monopodal support in the stance phase, developing eversion of the calcaneus and abduction of the forefoot linked to the hindfoot. The purpose of our research was to analyze the dynamic symmetry index in the lower limbs comparing patients with flatfoot and normal foot.Methods: A case-control study was carried out with a sample of 62 participants divided into two groups consisting of 31 participants were overweight with bilateral flatfoot and 31 participants with healthy feet. A portable plantar pressure platform with piezoresistive sensors was used to measure the load symmetry index in the lower limbs in the foot areas and gait phases.Results: Gait pattern analysis showed statistically significant differences in the symmetry index for lateral load (p = 0.004), the initial contact phase (p = 0.025) and the forefoot phase (p < 0.001).Conclusion: The adults were overweight with bilateral flatfoot evidenced alterations in the symmetry index in the lateral load and in the initial contact and flatfoot contact phases, showing greater instability in overweight adult flatfoot compared to the people with normal feet

Influence of the center of pressure on baropodometric gait pattern variations in the adult population with flatfoot: A case-control study

[Abstract] Background: Adult flatfoot is considered an alteration in the foot bone structure characterized by a decrease or collapse of the medial arch during static or dynamic balance in the gait pattern. The aim of our research was to analyze the center of pressure differences between the population with adult flatfoot and the population with normal feet. Methods: A case-control study involving 62 subjects was carried out on 31 adults with bilateral flatfoot and 31 healthy controls. The gait pattern analysis data were collected employing a complete portable baropodometric platform with piezoresistive sensors. Results: Gait pattern analysis showed statistically significant differences in the cases group, revealing lower levels in the left foot loading response of the stance phase in foot contact time (p = 0.016) and contact foot percentage (p = 0.019). Conclusion: The adult population with bilateral flatfoot evidenced higher contact time data in the total stance phase compared to the control group, which seems to be linked to the presence of foot deformity in the adult population