Mechanisms involved in the adsorption of bioinsecticides and strategies to enhance their passage through the insect midgut.

During the past decade a number of products have emerged from the effort to develop alternative biopesticidal technologies. The most recent approach to integrated pest management is based on the detection of new genes encoding for polypeptides with potential insecticidal activity, with a particular attention to biopesticides derived from viruses, microorganisms, fungi, plants and insects. Bacteria and viruses themselves can be used for biological control purpose. In most cases, biopesticides are molecules that have haemocoelic targets and must pass undegraded the gut barrier in order to exert their activity. Therefore for an effective oral delivery of these molecules it will be crucial to develop basic information on the molecular mechanisms mediating the absorption of proteins and peptides by the insect gut and to develop strategies to facilitate their passage through the midgut barrier. These issues have been the subject of my research project during these three years. In particular, I characterized the strategies adopted by Junonia coenia densovirus (JcDNV) to cross the midgut barrier of the lepidopteran pest Spodoptera frugiperda. I also studied a possible mechanism to increase the protein uptake in insect midgut characterizing the mechanisms involved in the internalization of selected Cell Penetrating Peptides (Tat, Penetratin, L-oligoarginine and D-oligoarginine) through the plasma membrane of lepidopteran larveae midgut cells. Even though these two aspects represent the main part of my PhD project, I have also collaborated to the study devoted to the identification of strategies to increase the permeability of the paracellular route

classification of oncologic data with genetic programming

Discovering the models explaining the hidden relationship between genetic material and tumor pathologies is one of the most important open challenges in biology and medicine. Given the large amount of data made available by the DNA Microarray technique, Machine Learning is becoming a popular tool for this kind of investigations. In the last few years, we have been particularly involved in the study of Genetic Programming for mining large sets of biomedical data. In this paper, we present a comparison between four variants of Genetic Programming for the classification of two different oncologic datasets: the first one contains data from healthy colon tissues and colon tissues affected by cancer; the second one contains data from patients affected by two kinds of leukemia (acute myeloid leukemia and acute lymphoblastic leukemia). We report experimental results obtained using two different fitness criteria: the receiver operating characteristic and the percentage of correctly classified instances. These results, and their comparison with the ones obtained by three nonevolutionary Machine Learning methods (Support Vector Machines, MultiBoosting, and Random Forests) on the same data, seem to hint that Genetic Programming is a promising technique for this kind of classification

The promotion of mature theory of mind skills in educational settings: a mini-review

After formal school entry, theory of mind development encounters a blooming period of growth intertwined with social and academic achievements and challenges. Within this framework, in last years researchers have proposed training programs to foster mature ToM skills, but also, to test causal pathways for the role that ToM development may have in broader cognitive and social outcomes. In the current mini-review we examine which training programs have been developed so far to enhance three key aspects of mature ToM skills: second-order false belief reasoning, the ability to put one’s own ToM knowledge into use, and the mentalization of thoughts and emotions. We also illustrate effects of these activities on intra- and inter- personal competence. In its conclusion the paper provides considerations of both first achievements of research in this area and gaps to be addressed in future works

Teachers during the COVID-19 Era: The Mediation Role Played by Mentalizing Ability on the Relationship between Depressive Symptoms, Anxious Trait, and Job Burnout

Background: The COVID-19 outbreak caused severe changes in school activities over the past two years. Teachers underwent a re-planning of their teaching approaches, shifting from face-to-face teaching formats to remote ones. These challenges resulted in high levels of burnout. The identification of risk/protective factors contributing to burnout is crucial in order to inform intervention programs. Thus, we hypothesized a mediation role of teachers’ mentalizing ability (processing of emotions, a component of mentalized affectivity) on the relationship between depression, anxiety, and depersonalization (burnout dimension). Two reverse models were computed. Job satisfaction, teachers’ age and gender, school grade, and length of teaching experience served as covariates. Methods: 466 (M(sd) = 46.2 (10.4) years) online questionnaires were completed by Italian teachers of primary (n = 204) and middle (n = 242) schools. Measures of burnout, depression, anxiety, and mentalization were administered. Results: The findings corroborated our hypotheses: in all models, processing emotions served as a mediator on the relationship between depression, anxiety, and depersonalization, and on the reciprocal one. Job satisfaction positively impacted processing emotion, and negatively impacted depression and depersonalization; women teachers reported high levels of the anxious trait. Conclusions: Overall, it can be concluded that the ability to mentalize has a beneficial impact on teachers’ well-being. Policymaking, clinical, and research implications were discussed

Internalizing and externalizing symptoms in children during the COVID-19 pandemic: a systematic mixed studies review

Introduction: Given the vulnerability of children during the COVID-19 pandemic, paying close attention to their wellbeing at the time is warranted. The present protocol-based systematic mixed-studies review examines papers published during 2020–2022, focusing on the impact of the COVID-19 pandemic on children's internalizing/externalizing symptoms and the determinants thereof. Method: PROSPERO: CRD42022385284. Five databases were searched and the PRISMA diagram was applied. The inclusion criteria were: papers published in English in peer-reviewed journals; papers published between January 2020 and October 2022 involving children aged 5–13 years; qualitative, quantitative, and mixed studies. The standardized Mixed Method Appraisal Tool protocol was used to appraise the quality of the studies. Results: Thirty-four studies involving 40,976 participants in total were analyzed. Their principal characteristics were tabulated. The results showed that children's internalizing/externalizing symptoms increased during the pandemic, largely as a result of disengagement from play activities and excessive use of the internet. Girls showed more internalizing symptoms and boys more externalizing symptoms. Distress was the strongest parental factor mediating children's internalizing/externalizing symptoms. The quality of the studies was appraised as low (n = 12), medium (n = 12), and high (n = 10). Conclusion: Gender-based interventions should be designed for children and parents. The studies reviewed were cross-sectional, so long-term patterns and outcomes could not be predicted. Future researchers might consider a longitudinal approach to determine the long-term effects of the pandemic on children's internalizing and externalizing symptoms

The black pedagogy scale: A new task to explore educational practices for children's well-being

The present contribute focuses on the concept of "Black Pedagogy" (Rutschky, 1977; ISBN: 3548356702), meant as a set of educational practices assimilable into those that nowadays are included in the frame of physical and psychological maltreatment (e.g., corporal punishment, frightening children, etc.). The purpose of this work is to present our operationalization proposal of the concept and the results deriving from a first validation of the "Black Pedagogy Scale". The questionnaire was administered to 374 Italian university students in their university classrooms (pilot study with double administration) and to 830 Italian adults, parents of primary school-aged children, through an online survey platform (main study). In the pilot study, explorative analyses, paired-samples t-test and ML EFA (with Varimax rotation) were performed. In the main study, proprieties of the refined instrument and relations between the construct of Black Pedagogy and demographics were explored. The Black Pedagogy Scale (α > .8) resulted composed by three factors, consistently with what was initially hypothesized: "Values of Black Pedagogy" (var. 18.7%), "Education of children over time" (var. 10.6%) "Methods of Black Pedagogy" (var. 8.6%). Participants resulted more in agreement with Black Pedagogy's values rather than with its methods, and those with higher educational qualification showed less agreement with the construct, F(2, 813) = 28.22, p < .001, η² = .065. The possible legacy of a Black Pedagogy's forma mentis can contribute to explain why some detrimental disciplinary practices are culturally deemed as acceptable. Results suggest designing interventions focused on educational values to discourage such practices

Stevens-Johnson induced by imiquimod 5% cream: a case report

Imiquimod 5% cream is an approved treatment for actinic keratoses, superficial basal cell carcinomas and anogenital warts. Severe systemic side effects associated to imiquimod 5% cream are rare, although few cases of erythema multiforme and Stevens-Johnson syndrome have been described. We present a case of Stevens-Johnson syndrome associated to topical treatment with imiquimod of two superficial basal cell carcinomas

Inhibition of de novo ceramide biosynthesis affects aging phenotype in an in vitro model of neuronal senescence.

Although aging is considered to be an unavoidable event, recent experimental evidence suggests that the process can be counteracted. Intracellular calcium (Ca2+i) dyshomeostasis, mitochondrial dysfunction, oxidative stress, and lipid dysregulation are critical factors that contribute to senescence-related processes. Ceramides, a pleiotropic class of sphingolipids, are important mediators of cellular senescence, but their role in neuronal aging is still largely unexplored. In this study, we investigated the effects of L-cycloserine (L-CS), an inhibitor of thede novoceramide biosynthesis, on the aging phenotype of cortical neurons cultured for 22 days, a setting employed as anin vitromodel of senescence. Our findings indicate that, compared to control cultures, 'aged' neurons display dysregulation of [Ca2+]ilevels, mitochondrial dysfunction, increased generation of reactive oxygen species (ROS), altered synaptic activity as well as the activation of neuronal death-related molecules. Treatment with L-CS positively affected the senescent phenotype, a result associated with recovery of neuronal [Ca2+]isignaling and reduction of mitochondrial dysfunction and ROS generation. The results suggest that thede novoceramide biosynthesis represents a critical intermediate in the molecular and functional cascade leading to neuronal senescence and identify ceramide biosynthesis inhibitors as promising pharmacological tools to decrease age-related neuronal dysfunctions

Long-term monitoring for the surveillance of the conservation status of Tursiops truncatus in an EU Natura2000 site in the Mediterranean Sea. A pilot study in the Tuscan Archipelago

A pilot study, using the dataset from the research network ‘FLT Med Net’, which regularly monitors transborder regions in the Mediterranean Sea using ferries as platform for systematic surveys, was undertaken to assess common bottlenose dolphin range and population trends within the Natura 2000 EU marine site “Tutela del Tursiops truncatus”. The site was recently designated by the Tuscany Region (Italy) within the requirement of the EU Habitats Directive. In order to evaluate the conservation status of bottlenose dolphin according to the surveillance scheme of the Directive, two six-year periods (2007-2012; 2013-2018) were compared to assess trends in distribution-occurrence (range); Sightings Per Unit of Effort and Density (population). In total, 18146 NM were surveyed along two fixed transects, recording 90 sightings of Tursiops truncatus and a total of 268 specimens. Between the two periods, slight but not statistically significant differences were assessed, with decreasing trend in range and population  of the species; no variation was detected in mean group sizes. Travelling was the most common behaviour, and juveniles were present in 20% of the sightings, concentrated during spring and summer. The consistent FLT Med Net dataset was found to be appropriate to evaluate important parameters for the assessment of trends in the conservation status of Tursiops truncatus at the Natura2000 site scale.

Fairness Norms and Theory of Mind in an Ultimatum Game: Judgments, Offers, and Decisions in School-Aged Children

The sensitivity to fairness undergoes relevant changes across development. Whether such changes depend on primary inequity aversion or on sensitivity to a social norm of fairness is still debated. Using a modified version of the Ultimatum Game that creates informational asymmetries between Proposer and Responder, a previous study showed that both perceptions of fairness and fair behavior depend upon normative expectations, i.e., beliefs about what others expect one should do in a specific situation. Individuals tend to comply with the norm when risking sanctions, but disregard the norm when violations are undetectable. Using the same methodology with children aged 8-10 years, the present study shows that children's beliefs and behaviors differ from what is observed in adults. Playing as Proposers, children show a self-serving bias only when there is a clear informational asymmetry. Playing as Responders, they show a remarkable discrepancy between their normative judgment about fair procedures (a coin toss to determine the offer) and their behavior (rejection of an unfair offer derived from the coin toss), supporting the existence of an outcome bias effect. Finally, our results reveal no influence of theory of mind on children's decision-making behavior