187 research outputs found

    Vibratory Loads Data from a Wind-Tunnel Test of Structurally Tailored Model Helicopter Rotors

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    An experimental study was conducted in the Langley Transonic Dynamics Tunnel to investigate the use of a Bell Helicopter Textron (BHT) rotor structural tailoring concept, known as rotor nodalization, in conjunction with advanced blade aerodynamics as well as to evaluate rotor blade aerodynamic design methodologies. A 1/5-size, four-bladed bearingless hub, three sets of Mach-scaled model rotor blades were tested in forward flight from transition up to an advance ratio of 0.35. The data presented pertain only to the evaluation of the structural tailoring concept and consist of fixed-system and rotating system vibratory loads. These data will be useful for evaluating the effects of tailoring blade structural properties on fixed-system vibratory loads, as well as validating analyses used in the design of advanced rotor systems

    Telomere dynamics and fusion of critically shortened telomeres in plants lacking DNA ligase IV

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    In the absence of the telomerase, telomeres undergo progressive shortening and are ultimately recruited into end-to-end chromosome fusions via the non-homologous end joining (NHEJ) double-strand break repair pathway. Previously, we showed that fusion of critically shortened telomeres in Arabidopsis proceeds with approximately the same efficiency in the presence or absence of KU70, a key component of NHEJ. Here we report that DNA ligase IV (LIG4) is also not essential for telomere joining. We observed only a modest decrease (3-fold) in the frequency of chromosome fusions in triple tert ku70 lig4 mutants versus tert ku70 or tert. Sequence analysis revealed that, relative to tert ku70, chromosome fusion junctions in tert ku70 lig4 mutants contained less microhomology and less telomeric DNA. These findings argue that the KU-LIG4 independent end-joining pathway is less efficient and mechanistically distinct from KU-independent NHEJ. Strikingly, in all the genetic backgrounds we tested, chromosome fusions are initiated when the shortest telomere in the population reaches ∼1 kb, implying that this size represents a critical threshold that heralds a detrimental structural transition. These data reveal the transitory nature of telomere stability, and the robust and flexible nature of DNA repair mechanisms elicited by telomere dysfunction

    Molecular Assisted Identification Reveals Hidden Red Algae Diversity from the Burica Peninsula, Pacific Panama

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    The marine flora of Panama harbors a rich diversity of green, red and brown algae,and despite chronic understudy, it is reported as the second most diverse marine flora along thePacific Central American coast, with 174 macroalgal species. Extensive new collections and molecularassisted identification (MAI) by an international team of researchers has revealed an even greaterdiversity for this country. Here, the intertidal and shallow subtidal marine flora of the remoteBurica Peninsula is introduced. This area is characterized by an uplifted extensive intertidal flatcomposed of firm, sedimentary benthos known as mudrock, on which abundant algal communitiesthrive, even during extended periods of exposure. A collection of nearly 200 brown, green and redmacroalgae specimens representing the first marine floristic inventory of this region was made inJanuary 2011, and results of analyses of 45 foliose red algae specimens are presented. DNA sequencedata for several loci (rbcL-3P; COI-5P; UPA) have been generated for molecular assisted identificationand to guide morphological assessments. Twenty-six species were identified among the specimensincluding 21 new Pacific Panama records, as well as previously unrealized transisthmian distributions,and two new species, Neorubra parvolacertoides sp. nov. and Grateloupia irregularis sp. novNational Science Foundation/[0743334]/NSF/Estados UnidosUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Ciencias del Mar y Limnología (CIMAR)UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí

    Determinants of Restaurant Systematic Risk: A Reexamination

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    This study reexamines determinants of the systematic risk or beta of restaurant firms based on the financial data of 75 U.S. restaurant firms from 1996 through 1999. Our weighted least-squares regression analysis found that restaurant systematic risk correlated negatively with assets turnover but positively with quick ratio. The findings suggest that high efficiency in generating sales revenue helps lower the systematic risk, while excess liquidity tends to increase the risk

    Estimating fine-root production by tree species and understorey functional groups in two contrasting peatland forests

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    Background and aims Estimation of root-mediated carbon fluxes in forested peatlands is needed for understanding ecosystem functioning and supporting greenhouse gas inventories. Here, we aim to determine the optimal methodology for utilizing ingrowth cores in estimating annual fine-root production (FRP) and its vertical distribution in trees, shrubs and herbs. Methods We used 3-year data obtained with modified ingrowth core method and tested two calculation methods: 'ingrowth-dividing' and `ingrowth-subtracting'. Results The ingrowth-dividing method combined with a 2-year incubation of ingrowth cores can be used for the 'best estimate' of FRP. The FRP in the nutrient-rich fen forest (561 g m(-2)) was more than twice that in the nutrient-poor bog forest (244 g m(-2)). Most FRP occurred in the top 20-cm layer (76-82 %). Tree FRP accounted for 71 % of total FRP in the bog and 94 % in the fen forests, respectively, following the aboveground vegetation patterns; however, in fen forest the proportions of spruce and birch in FRP were higher than their proportions in stand basal area. Conclusions Our methodology may be used to study peatland FRP patterns more widely and will reduce the volume of labour-intensive work, but will benefit from verification with other methods, as is the case in all in situ FRP studies.Peer reviewe

    Decisions that hasten death: double effect and the experiences of physicians in Australia

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    BACKGROUND: In Australian end-of-life care, practicing euthanasia or physician-assisted suicide is illegal. Despite this, death hastening practices are common across medical settings. Practices can be clandestine or overt but in many instances physicians are forced to seek protection behind ambiguous medico-legal imperatives such as the Principle of Double Effect. Moreover, the way they conceptualise and experience such practices is inconsistent. To complement the available statistical data, the purpose of this study was to understand the reasoning behind how and why physicians in Australia will hasten death. METHOD: A qualitative investigation was focused on palliative and critical/acute settings. A thematic analysis was conducted on semi-structured in-depth interviews with 13 specialist physicians. Attention was given to eliciting meanings and experiences in Australian end-of-life care. RESULTS: Highlighting the importance of a multidimensional approach, physicians negotiated multiple influences when death was regarded as hastened. The way they understood and experienced end-of-life care practices were affected by politico-religious and cultural influences, medico-legal imperatives, and personal values and beliefs. Interpersonal and intrapsychic aspects further emphasised the emotional and psychological investment physicians have with patients and others. In most cases death occurred as a result of treating suffering, and sometimes to fulfil the wishes of patients and others who requested death. Experience was especially subject to the efficacy with which physicians negotiated complex but context-specific situations, and was reflective of how they considered a good death. Although many were compelled to draw on the Principle of Double Effect, every physician reported its inadequacy as a medico-legal guideline. CONCLUSIONS: The Principle of Double Effect, as a simplistic and generalised guideline, was identified as a convenient mechanism to protect physicians who inadvertently or intentionally hastened death. But its narrow focus on the physician’s intent illuminated how easily it may be manipulated, thus impairing transparency and a physician’s capacity for honesty. It is suggested the concept of “force majeure” be examined for its applicability in Australian medical end-of-life law where, consistent with a multidimensional and complex world, a physician’s motivations can also be understood in terms of the emotional and psychological pressures they face in situations that hasten death
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