Struktur Caring Pada Mahasiswa Keperawatan (Caring Structure In Nursing Students)

The caring attitude of students tends to be lacking so that it can have an impact on the quality of nursing care services to patients during clinical practice. The purpose of this study was to describe the Strukturons of caring for nursing students. Methods This type of research was analytic observational with a cross sectional design. The research population was all students of the nursing faculty at a private campus in Surabaya as many as 122 people, where the sampling technique used total sampling. The research instrument used a caring questionnaire that has been tested for validity and reliability. Results The majority of respondents have a good caring attitude as many as 84 respondents (69%). The results of the analysis with ordinal regression obtained that the Strukturon of knowing in caring has the largest Nagelkerke coefficient, which is 0.528, which means that knowing has a large influence of 52.8% on students' caring attitudes. Discussion The caring attitude of students on the Strukturon of knowing has the greatest value because at this stage it is often done by students to obtain patient data as nursing assessment data that is useful for determining patient problems. Conclusion Five Strukturons of caring have a great influence on the caring attitude of students

In Vitro Propagation of Lily (Lilium longiflorum Thunb.) with Growth Regulators BAP and NAA

Lily plant (Lilium longiflorum Thunb.) is an ornamental and cut flower. The demand for cut lily flowers has a promising demand, but the availability of seedling is llimited. This study consisted of in vitro germination of lily seeds on ½ Murashige & Skoog (MS) medium, multiplication of base and shoot-tip explants in various treatments of MS medium with the combination of plant growtht regulator (PGR) BAP and NAA. The aims of this research were to obtain the appropriate combination of media and the parts of explants of growing shoots, roots and callus.The combinations of MS medium with BAP and NAA are (a)control 0:0 mg L-1, (b)0:0.1 mg L-1, (c)0:1 mg L-1, (d)0.1:0 mg L-1, (e)0.1:0.1 mg L-1, (f)(0.1:1 mg L-1), (g)1:0 mg L-1, (h)0.1:1 mg L-1, (i)1:1 mg L-1. The research were arranged as factorial design with two factor in Randomized Complete Block Design (RCBD). The first factors were combination of BAP and NAA, and the second one was the types of explant (base part of shoot and shoot tip). Lily seeds were succesfully germinated as much as 58%. Medium c and i succesfully induced explants to grow buds, roots and callus. Medium c induced shoots, roots and callus, meanwhile medium i induced roots and callus. Percentage of response growth of shoot base explants were 11.1% shoot growth and 13.3% roots, while shoot tip explants only showed 6.7% root growth without shoot growth. The growth of callus showed the same results on the base and shoot tip explants (4.4%).Tanaman lili (Lilium longiflorum Thunb.) merupakan tanaman hias dan bunga potong. Permintaan bunga potong lili sangat menjanjikan tetapi ketersediaan bibit masih terbatas. Penelitian ini terdiri dari perkecambahan biji lili secara in vitro pada media ½ Murashige & Skoog (MS), multiplikasi bagian eksplan pangkal dan ujung tunas pada berbagai perlakuan media MS kombinasi ZPT BAP dan NAA.  Tujuannya untuk mendapatkan kombinasi media yang tepat dan mengetahui persentase tertinggi bagian eksplan yang mampu tumbuh tunas, akar dan kalus. Kombinasi media MS dengan BAP dan NAA yaitu (a) kontrol 0:0 mg L-1, (b) 0:0.1 mg L-1, (c) 0:1 mg L-1, (d) 0.1:0 mg L-1, (e) 0.1:0.1 mg L-1, (f) 0.1:1 mg L-1, (g) 1:0 mg L-1, (h) 0.1:1 mg L-1, (i) 1;1 mg L-1. Penelitian ini disusun dalam RAL faktorial dua faktor yaitu kombinasi pemberian BAP dan NAA, multiplikasi eksplan bagian pangkal dan ujung tunas. Biji lili mampu berkecambah sebanyak 58%. Media c dan i yang mampu menginduksi eksplan untuk tumbuh tunas, akar dan kalus. Media c mampu menginduksi tunas, akar dan kalus, media i mampu menginduksi akar dan kalus. Persentase respon pertumbuhan eksplan bagian pangkal tunas yaitu pertumbuhan tunas 11.1% dan akar 13.3%, sedangkan eksplan bagian ujung tunas menunjukkan pertumbuhan akar 6.7% tanpa pertumbuhan tunas. Pertumbuhan kalus menunjukkan hasil yang sama pada eksplan pangkal dan ujung tunas yaitu 4.4%

Pelatihan Pengisian SPT Tahunan Orang Pribadi (OP) pada Mahasiswa Prodi Akuntansi Universitas Bumigora

The community service program implemented at Bumigora University aims to: carry out training activities with all students of the Accounting study program, in training on Procedures for Filling in Annual Personal Income Tax Return. The service material presented was training on reporting and filling out the Annual Personal Income Tax Return in hall 3 of the Bumigora University building. The method used in this activity namely by giving lectures / giving material followed by discussion and question and answer responsibility between presenters and participants in filling out the Annual Personal Income Tax Return. Training results The completion of the Annual Personal Income Tax Return is expected to make a significant contribution in the form of knowledge and skills that can be used as provisions in readiness to become a skilled workforce in the field of Accounting and Taxation to be able to compete in the world of work

Pendampingan Pelaporan SPT Tahunan Berbasis E-filing Bagi Pedagang Kelontong di tengah Wabah Covid-19

Government appeals to stay at home and restrictions on the delivery of public services do make people less comfortable in receiving public services, but this is a policy currently taken by the government is an effort to limit or stop the spread of Corona Virus. based on the instructions given, DJP (Direktorat Jenderal Pajak) then stop reporting directly and lead to the use of e-filing and extend the tax reporting period that should end on 31 March 2020 to 30 April 2020. Termination is what later became a problem experienced by taxpayers, especially the grocery traders in Gerung Market, Gerung District, West Lombok Regency, where the average trader was very unfamiliar with the use of electronics, especially the use of computers and the internet. By coordinating with an account representative, the servant provides assistance to traders who wish to report their annual tax return online while still paying attention to social distancing or maintaining a safe distance from participants. because of the adaptations made so that not all registered participants can be accompanied, namely only 50% of participants who successfully report online, 20% of participants are reported manually and the rest are still waiting for further policies

Pollen Viability and Microspore Culture in Three Broccoli Cultivars (Brassica oleracea L. var. italica Plenck)

Broccoli is a high value vegetable crop in Indonesia, however production is low due to limited number of suitable cultivars, so, breeding hybrid broccoli for warm climate is important.  The first step in hybridization is providing homozygote parent plants which can be done efficiently via microspore culture.  The objectives of this study were to determine 1) bud size that produce uninucleate microspore stage appropriate for culture; 2) pollen viability, 3) microspore development, in three broccoli cultivars (‘BL 10001’, ‘Royal Green’ and ‘Green Magic’). Various bud size (1 – 5 cm) was squashed and observed microscopically to determine bud size containing uninucleate microspore.  Pollen viability was determined by IKI staining and pollen germination method.  Chromosome number was counted on root tips using squash method with aceto-orcein stain. Various heat treatment schemes were conducted to induce microspreo development.  Result showed uninucleate microspore derived from 2-3 mm and 3-4 mm bud length of ‘BL-10001’ and ‘Royal Green’ was responsive for microspore development in culture.  Pollen viability varied among cultivars, 78-87% on IKI method and 15-16% on germination test. Microspore culture showed different embryogenesis response; pollen-like structure was produced by ‘BL 10001’

Genetic Diversity and Phylogenetic of Longtail Tuna (Thunnus tonggol) Landed in Pabean Fish Market, Surabaya

Indonesia is the biggest tuna exporter in Southeast Asia. With a high number of tuna catch, it is worried that the catch will decrease tuna population, specifically longtail tuna. To anticipate the decrease, there needs to be a conservation program to protect longtail tunas from scarcity. One method used to protect longtail tuna is by genetic conservation. The aim of this research is to understand the genetic and phylogenetic variety of the longtail tuna in Pabean Surabaya Fish Market. The polymerase chain reaction was used to amplify segment of the mitochondrial control region gene from members of these sample, used forward primer CRK 5’-AGCTC AGCGC CAGAG CGCCG GTCTT GTAAA-3’ and reverse primer CRE 5’-CCTGA AGTAG GAACC AGATG-3’. Based on the sequencing process, 28 out of 29 samples longtail tuna  were analyzed successfully. The results of the 28 sample analysis of longtail tuna based on its genetic variety and phylogenetic tree reconstruction showed a haplotype variety (Hd) score of 1,00000, and nucleotide (π) variety of 0,1939. Genetic variety value showed that longtail tuna has great adaption capabilities toward environmental changes time to time. Phylogenetic tree reconstruction results showed 7 clades with a genetic range of 0,005 – 0,035, which shows that all samples are closely related. The results of this study can be used as basic information in forming regulations on longtail tuna sustainable management and genetic conservation

Inisiasi Kalus Bangle (Zingiber purpureum Roscoe) pada Beberapa Kombinasi 2.4-D dan Kinetin

Bangle (Zingiber purpureum Roscoe) termasuk keluarga Zingiberaceae yang dimanfaatkan sebagai tanaman obat. Penelitian bertujuan untuk mengetahui pengaruh 2.4-D dan kinetin pada inisiasi kalus bangle. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Tumbuhan, Prodi Biologi, Fakultas MIPA, Universitas Udayana dari bulan Februari hingga bulan Mei 2023. Desain percobaan yaitu Rancangan Acak Lengkap (RAL) dua faktor dan lima ulangan. Faktor pertama konsentrasi 2.4-D (0, 1 dan 2 ppm) dan faktor kedua konsentrasi kinetin (0, 0.5 dan 1 ppm). Variabel yang diamati meliputi waktu inisiasi kalus, persentase pembentukan kalus, dan morfologi kalus. Hasil penelitian menunjukkan 1 ppm 2.4-D - 0.5 ppm kinetin menghasilkan waktu inisiasi kalus tercepat (10.8 HST) dan persentase pembentukan kalus tertinggi (100%), sementara 1 ppm 2.4-D - 0.5 ppm kinetin dan 2 ppm 2.4-D - 0.5 ppm kinetin menghasilkan morfologi kalus yang baik dan embriogenik (warna putih dengan tekstur remah). Kata Kunci: auksin, sitokinin, tanaman obat, ZingiberaceaeBangle (Zingiber purpureum Roscoe) is belonging to the Zingiberaceae family that has been utilized as a medicinal plant. The objective of the research was to evaluate the effects of 2.4-D and kinetin on bangle’s callus initiation. This research was conducted at the Plant Tissue Culture Laboratory, Biology Study Program, Faculty of Mathematics and Natural Science, Udayana University from February until May 2023. This experiment was arranged using a Completely Randomized Design (CRD) with two factors and repeated five times. The first factor was 2.4-D’s concentration (0, 1 and 2 ppm) and the second factor was kinetin’s concentration (0, 0.5 and 1 ppm). Variables observed were callus initiation time, percentage of callus formation, and callus morphology. The result shows that 1 ppm 2.4-D - 0.5 ppm kinetin produce the fastest callus initiation time (10.8 days) and the highest callus formation percentage (100%), whereas 1 ppm 2.4-D - 0.5 ppm kinetin and 2 ppm 2.4-D - 0.5 ppm kinetin produce embriogenic callus morphology (white color and friable texture). Keywords: auxin, cytokinin, medicinal plant, Zingiberacea

EMBRIOGENESIS PADA TANAMAN TEBU (Saccharum sp.) VARIETAS BULULAWANG DENGAN TEKNIK KULTUR MIKROSPORA

Tanaman tebu adalah keluarga poace yang berpotensi menghasilkan nilai ekonomi tinggi karena manfaatnya sebagai produk unggulan dalam menghasilkan gula serta bioetanol yang banyak digunakan oleh masyarakat luas. Untuk itu perlu upaya pemuliaan tanaman yang cepat untuk memenuhi kebutuhan pasar. Teknik kultur mikrospora merupakan aplikasi bioteknologi yang efisien dalam menghasilkan tanaman haploid. Teknik ini belum berhasil diaplikasikan pada tanaman tebu sehingga dalam penelitian ini bertujuan untuk memicu embriogenesis mikrospora tebu dengan aplikasi kultur mikrospora pada varietas bululawang. Metode yang digunakan yaitu dengan stress perlakukan perendaman mannitol 0.3M sebelum isolasi kultur pada lama perendaman yang berbeda. Analisis data menggunakan uji anova dan uji duncan. Hasil yang diperoleh yaitu Perlakuan stress yang diberikan berhasil memicu embriogenesis mikrospora dengan lama perendaman anther optimum pada 7 hari pada mannitol 0.3

Optimasi Metode Cryotherapy untuk Mengeliminasi Virus pada Tunas Kentang In Vitro (Optimation of Cryotherapy Method to Eliminate Virus on In Vitro Potato Shoot Tips)

Penggunaan benih kentang generasi awal dan bebas virus merupakan kunci keberhasilan produksi kentang berkualitas. Cryotherapy (perendaman dalam nitrogen cair) merupakan teknik terbaru untuk mengeliminasi virus pada benih kentang. Salah satu kendala dalam penerapan teknologi cryotherapy ialah tingkat daya hidup eksplan yang masih rendah. Penelitian ini bertujuan untuk mengetahui efektifitas teknik enkapsulasi-dehidrasi untuk mendapatkan tunas yang sehat setelah perendaman dalam nitrogen cair. Ujung tunas in vitro ukuran 2–3 mm dari empat genotipe kentang di prakultur selama 3 hari secara bertahap pada media MS dengan penambahan gula 0,25 M, 0,5 M, dan 0, 75 M. Kemudian tunas dienkapsulasi, didehidrasi selama 5 jam, lalu direndam dalam nitrogen cair selama 60 menit lalu dihangatkan kembali dalam waterbath selama 3 menit. Tunas dalam kapsul kemudian dikulturkan pada media MS +30 g/l sukrosa + 8 g/l agar + 0,4 mg/l BAP + 1 mg/l GA3 untuk pemulihan, lalu dipelihara di ruang kultur dengan suhu 24oC. Daya hidup ujung tunas diamati pada minggu ke-8 dengan menggunakan kriteria skoring sebagai berikut: (1) pemutihan jaringan dan tidak ada respons pertumbuhan, (2) kalus mencokelat, (3) kalus hijau, (4) tumbuh tunas, dan (5) planlet sehat. Hasil penelitian menunjukkan daya hidup ujung tunas bervariasi antargenotipe. Skor daya hidup berkisar 1–2 (frekuensi 2–10) pada perlakuan nitrogen cair, yang menunjukkan tidak ada respons pertumbuhan tunas, beberapa memperlihatkan pertumbuhan kalus. Tunas pada perlakuan kontrol (tanpa perendaman dalam nitrogen cair) menunjukkan skor daya hidup 5 (frekuensi 1–7), di mana ujung tunas mampu beregenerasi menjadi planlet.KeywordsCryopreservation; Solanum tuberosum; Kultur jaringan tanaman; Eliminasi virusAbstractVirus-free, early generation seed is a key in the production of high quality potatoes. Cryotherapy (exposure to liquid nitrogen) is a new and promising method of virus elimination. One bottleneck in cryotheraphy method is survival of the explants after treatment with liquid nitrogen. This study investigated the effectiveness of enkapsulasi-dehidrasi method to obtain survival explants. Shoot tips were precultured for 3 days in MS media with sucrose addition of 0.25 M, 0.5 M and 0.75 M. Shoot tips were then encapsulate, dehydrate for 5 hours, expose to liquid nitrogen for 60 minutes and rewarm in waterbath for 3 minutes. Beads with shoot tips were then cultured in MS media + 30 g/l sucrose + 8 g/l agar + 0.4 mg/l BAP + 1 mg/l GA3 for recovery, and placed in 24oC culture room. Shoot tip survival was assessed at 8 weeks using the following scoring criteria: (1) tissue bleaching and no growth response, (2) brown callus, (3) green callus, (4) shoot growth, and (5) plantlet establishment. Survival was varied among genotypes. Survival scored between 1–2 (frequency 2–10) on liquid nitrogen treatment, showing shoot tips are mostly has no growth response, only some callus growth. Shoot tips on control treatment (without exposure in liquid nitrogen) shows survival scored 5 (frequency 1–7), i.e. shoot tips able to regenerate into plantlets