12 research outputs found
Control of crystalline orientation of hot forged Nd-Fe-B magnet
Nd-Fe-B powder obtained by melt spinning was compacted, and then forged to produce a magnet with even magnetic properties. There is a strong correlation between the strain introduced to the magnet while it is being forged and the obtained magnetic properties. In general, such strain is unevenly introduced to the magnet owing to the friction generated between the mold and magnet. Consequently, the obtained magnetic properties also become uneven. We obtained even magnetic properties by defining the optimum shape before forging with the use of finite element method (FEM), choosing the optimum mold material and lubricant, and introducing strain evenly. From the viewpoint of decreasing friction, we used a smooth-surface ceramic mold and a graphite-based lubricant. If a ceramic mold with a normal shape is used, its corners are easily broken during forging because stress is concentrated at these corners. Ceramic molds are also extremely expensive. In this study, we configured a mold composed of ceramic plates in a simple shape. This approach successfully prevented the mold from being broken and kept the mold price low
SNHG5 promotes proliferation and induces apoptosis in melanoma by sponging miR-155
Background: Melanoma is the most common malignancy of skin cancer. Small nucleolar RNA host gene 5 (SNHG5), a long non-coding RNA (lncRNA), has been demonstrated to be abnormally expressed in multiple malignances. However, the roles and molecular mechanisms of SNHG5 in melanoma progression have not been well identified. Methods: RT-qPCR assays were used to detect the expression patterns of SNHG5 and microRNA-155 (miR-155). Cell proliferation was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays. Cell apoptosis rate was measured by flow cytometry via double-staining of fluorescein isothiocyanate (FITC)-labeled annexin V (Annexin V-FITC) and propidium iodide (PI). The interaction between SNHG5 and miR-155 was validated using bioinformatics analysis, subcellular fraction assay, luciferase assay and RNA immunoprecipitation (RIP) assay. A mouse model of melanoma was established to further verify the effect of SNHG5 on tumor growth in vivo. Results: SNHG5 expression was upregulated in melanoma tumor tissues and cell lines. Moreover, higher SNHG5 expression was associated with advanced pathogenic status and poor prognosis. Functional analysis showed that SNHG5 knockdown suppressed proliferation and facilitated apoptosis in melanoma cells. Mechanical exploration revealed that SNHG5 acted as a molecular sponge of miR-155 in melanoma cells. Restoration experiments delineated that miR-155 down-regulation partly abrogated SNHG5-knockdown-mediated anti-proliferation and pro-apoptosis effect in melanoma cells. In vivo assays further demonstrated that SNHG5 depletion hindered tumor growth through up-regulating miR-155 expression. Conclusion: SNHG5 promoted the development of melanoma by sponging miR-155 in vitro and in vivo, implying the important implication of lncRNAs in melanoma progression and providing a potential therapeutic target for melanoma