The petiole and leaf blade relationships for the determination oi phosphorus and zinc status of vineyards

For the determination of phosphorus and zinc nutrition in vineyards, petiole and leaf blade relationships of these nutrients and their ratios were investigated. Significant correlations were found at 1 % levels between the phosphorus contents in petioles and leaf blades (r = 0.931 **) and also between the zinc contents in leaf blades and petioles (r = 0.445**). From the regression equations of these relationships, 0.215 % for phosphorus and 43 ppm for zinc were obtained as the levels at which petiole and leaf blade nutritional contents were equal to each other.In the phosphorus rich vineyards, the petiole-P is higher than the leaf blade-P and in the zinc rich vineyards, the leaf blade-Zn is higher than the petiole-Zn. However, just the opposite situation happens in the poorly fertilized vineyards.Beziehungen zwischen Blattstiel und Blattspreite zur Beurteilung der Phosphor undZinkversorgung bei WeinrebenZur Beurteilung der Phosphor- und Zinkversorgung von Reben wurden der Gehalt dieser Nährstoffe im Blattstiel und in der Blattspreite sowie die gegenseitigen Beziehungen dieser Elemente untersucht.Es konnte eine statistisch gesicherte Korrelation (P = 1 %) zwischen Blattstiel und Blattspreite hinsichtlich des Phosphorgehaltes (r = 0,931 **) und des Zinkgehaltes (r = 0,445**) aufgezeigt werden. Aus den Regressionsgeraden war zu entnehmen, daß Phosphor und Zink in den beiden Blatteilen dann in gleichen Mengen vorlagen, wenn der Gehalt an Phosphor 0,215 % und an Zink 43 ppm betrug.Bei den mit Phosphor genügend versorgten Weinbergen zeigte die Blattspreite einen niedrigeren Phosphorgehalt als der Blattstiel, während bei ausreichender Zinkversorgung in der Blattspreite mehr Zink als im Blattstiel enthalten war. Im Falle der ungenügend versorgten Weinberge lagen zwischen den beiden Nährstoffen umgekehrte Beziehungen vor

Identification of genes induced by BRCA1 in breast cancer cells

Cataloged from PDF version of article.Inherited mutations of the BRCA1 gene predispose to breast, ovarian, and other cancers. The role of the BRCA1 gene in the maintenance of chromosomal integrity is linked to a number of biological properties of its protein product, including transcriptional regulation. In the present study, we have used suppression subtractive hybridisation (SSH) to identify genes induced by BRCA1 by comparing control MCF7 breast carcinoma cells (driver) with MCF7 cells ectopically expressing BRCA1 (tester) and generated a forward subtracted cDNA library. We screened 500 putative positive clones from this library. Two hundred and ten of these clones were positive by differential screening with forward and reverse subtracted probes and the 65 cDNA clones which showed more than fivefold increase were selected for sequencing analysis. We clustered 46 different genes that share high homology with sequences in the GenBank/EMBL databases. Among these, 30 were genes whose function had been previously identified while the remaining 16 clones were genes with,unknown functions. Of particular interest, BRCA1 gene induces the expression of genes encoding DNA repair proteins RAD21 and MSH2, ERBB2/HER2 interacting protein ERBIN, meningioma-associated protein MAC30, and a candidate ovarian tumour-suppressor OVCA1. Northern and Western blot analyses confirmed that the expression of these five genes are up-regulated following BRCA1 overexpression in MCF7 and UBR60-bcl2 cells. This is the first study reporting a set of BRCA1-induced genes in breast carcinoma cells by the SSH technique. We suggest that some known genes identified in this study may provide new insights into the tumour-suppressor function of BRCA1. (C) 2002 Elsevier Science (USA). All rights reserved

Cancer Cell Cytotoxicities of 1-(4-Substitutedbenzoyl)-4-(4-chlorobenzhydryl) piperazine Derivatives

Cataloged from PDF version of article.A series of novel 1-(4-substitutedbenzoyl)-4-(4-chlorobenzhydryl)piperazine derivatives 5a-g was designed by a nucleophilic substitution reaction of 1-(4-chlorobenzhydryl) piperazine with various benzoyl chlorides and characterized by elemental analyses, IR and H-1 nuclear magnetic resonance spectra. Cytotoxicity of the compounds was demonstrated on cancer cell lines from liver (HUH7, FOCUS, MAHLAVU, HEPG2, HEP3B), breast (MCF7, BT20, T47D, CAMA-1), colon (HCT-116), gastric (KATO-3) and endometrial (MFE-296) cancer cell lines. Time-dependent cytotoxicity analysis of compound 5a indicated the long-term in situ stability of this compound. All compounds showed significant cell growth inhibitory activity on the selected cancer cell lines

A novel thiazolidine compound induces caspase-9 dependent apoptosis in cancer cells

Cataloged from PDF version of article.The forward chemogenomics strategy allowed us to identify a potent cytotoxic thiazolidine compound as an apoptosis-inducing agent. Chemical structures were designed around a thiazolidine ring, a structure already noted for its anticancer properties. Initially, we evaluated these novel compounds on liver, breast, colon and endometrial cancer cell lines. The compound 3 (ALC67) showed the strongest cytotoxic activity (IC50 ∼5 μM). Cell cycle analysis with ALC67 on liver cells revealed SubG1/G1 arrest bearing apoptosis. Furthermore we demonstrated that cytotoxicity of this compound was due to the activation of caspase-9 involved apoptotic pathway, which is death receptor independent. © 2012 Elsevier Ltd. All rights reserve

Dual functionality of conjugated polymer nanoparticles as an anticancer drug carrier and a fluorescent probe for cell imaging

Cataloged from PDF version of article.Multifunctional nanoparticles based on a green emitting, hydrophobic conjugated polymer, poly[(9,9-bis{propeny}fluorenyl-2,7-diyl)-co-(1,4- benzo-{2,1,3}-thiodiazole)] (PPFBT), that acts both as a fluorescent reporter and a matrix to accommodate an anti-cancer compound, camptothecin (CPT), were prepared, characterized and their potential as a fluorescent probe for cell imaging and as a drug delivery vehicle were evaluated via in vitro cell assays. The cell viability of human hepatocellular carcinoma cell line (Huh7) was investigated in the absence and presence of CPT with sulforhodamine B (SRB) and real-time cell electronic sensing (RT-CES) cytotoxicity assays

The Design and Cytotoxic Evaluation of Some 1-Aryl-3- isopropylamino-1-propanone Hydrochlorides towards Human Huh-7 Hepatoma Cells

Cataloged from PDF version of article.A series of 1-aryl-3-isopropylamino-1-propanone hydrochlorides 1 and a related heterocyclic analog 2 as candidate antineoplastic agents were prepared and the rationale for designing these compounds is presented. A specific objective in this study is the discovery of novel compounds possessing growth-inhibiting properties of hepatoma cells. The compounds in series 1 and 2 were prepared and their structures established unequivocally. X-ray crystallography of two representative compounds 1d and 1g were achieved. Over half of the compounds are more potent than 5-fluorouracil which is an established drug used in treating liver cancers. QSAR evaluations and molecular modeling studies were undertaken with a view to detecting some physicochemical parameters which govern cytotoxic potencies. A number of guidelines for amplification of the project have been formulated. A number of Mannich bases displayed greater potency than the reference drug 5-fluorouracil against human Huh-7 hepatoma cells. In particular, 1i emerged as a lead compound possessing 2.8 fold higher activity than that of the reference drug. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

A small library of chalcones induce liver cancer cell death through Akt phosphorylation inhibition

Hepatocellular carcinoma (HCC) ranks as the fifth most common and the second deadliest cancer worldwide. HCC is extremely resistant to the conventional chemotherapeutics. Hence, it is vital to develop new treatment options. Chalcones were previously shown to have anticancer activities in other cancer types. In this study, 11 chalcones along with quercetin, papaverin, catechin, Sorafenib and 5FU were analyzed for their bioactivities on 6 HCC cell lines and on dental pulp stem cells (DPSC) which differentiates into hepatocytes, and is used as a model for untransformed control cells. 3 of the chalcones (1, 9 and 11) were selected for further investigation due to their high cytotoxicity against liver cancer cells and compared to the other clinically established compounds. Chalcones did not show significant bioactivity ([Formula: see text]) on dental pulp stem cells. Cell cycle analysis revealed that these 3 chalcone-molecules induced SubG1/G1 arrest. Akt protein phosphorylation was inhibited by these molecules in PTEN deficient, drug resistant, mesenchymal like Mahlavu cells leading to the activation of p21 and the inhibition of NF[Formula: see text]B-p65 transcription factor. Hence the chalcones induced apoptotic cell death pathway through NF[Formula: see text]B-p65 inhibition. On the other hand, these molecules triggered p21 dependent activation of Rb protein and thereby inhibition of cell cycle and cell growth in liver cancer cells. Involvement of PI3K/Akt pathway hyperactivation was previously described in survival of liver cancer cells as carcinogenic event. Therefore, our results indicated that these chalcones can be considered as candidates for liver cancer therapeutics particularly when PI3K/Akt pathway involved in tumor development

The Effect of the Pairing Interaction on the Energies of Isobar Analog Resonances in 112−124^{112-124}Sb and Isospin Admixture in 100−124^{100-124}Sn Isotopes

In the present study, the effect of the pairing interaction and the isovector correlation between nucleons on the properties of the isobar analog resonances (IAR) in $^{112-124}$Sb isotopes and the isospin admixture in $^{100-124}$Sn isotopes is investigated within the framework of the quasiparticle random phase approximation (QRPA). The form of the interaction strength parameter is related to the shell model potential by restoring the isotopic invariance of the nuclear part of the total Hamiltonian. In this respect, the isospin admixtures in the $^{100-124}$Sn isotopes are calculated, and the dependence of the differential cross section and the volume integral $J_{F}$ for the Sn($^{3}$He,t)Sb reactions at E($^{3}$He)$=200$ MeV occurring by the excitation of IAR on mass number A is examined. Our results show that the calculated value for the isospin mixing in the $^{100}$Sn isotope is in good agreement with Colo et al.'s estimates $(4-5$, and the obtained values for the volume integral change within the error range of the value reported by Fujiwara et al. (53$\pm$5 MeV fm$^{3}$). Moreover, it is concluded that although the differential cross section of the isobar analog resonance for the ($^{3}$He,t) reactions is not sensitive to pairing correlations between nucleons, a considerable effect on the isospin admixtures in $N\approx Z$ isotopes can be seen with the presence of these correlations.Comment: 16 pages, 5 EPS figures and 2 tables, Late