REST is a hypoxia-responsive transcriptional repressor

Cellular exposure to hypoxia results in altered gene expression in a range of physiologic and pathophysiologic states. Discrete cohorts of genes can be either up- or down-regulated in response to hypoxia. While the Hypoxia-Inducible Factor (HIF) is the primary driver of hypoxia-induced adaptive gene expression, less is known about the signalling mechanisms regulating hypoxia-dependent gene repression. Using RNA-seq, we demonstrate that equivalent numbers of genes are induced and repressed in human embryonic kidney (HEK293) cells. We demonstrate that nuclear localization of the Repressor Element 1-Silencing Transcription factor (REST) is induced in hypoxia and that REST is responsible for regulating approximately 20% of the hypoxia-repressed genes. Using chromatin immunoprecipitation assays we demonstrate that REST-dependent gene repression is at least in part mediated by direct binding to the promoters of target genes. Based on these data, we propose that REST is a key mediator of gene repression in hypoxia

Taxis en Barcelona y uberización del sector

El cas consta de 2 documents: El cas per a l'alumnat i una Guia per al professorat amb pautes

AIF-regulated oxidative phosphorylation supports lung cancer development

International audienc

Palaeoenvironmental and chronological context of human occupations at El Cierro cave (Northern Spain) during the transition from the late Upper Pleistocene to the early Holocene

El Cierro Cave possesses one of the few sequences in SW Europe in which archaeological levels cover the transition from the late Pleistocene to the early Holocene. Information contributed by the palynological and anthracological studies indicates that this transition was marked by a steady expansion of broadleaf woodland and a reduction in herbaceous-shrub communities. Archaeofaunal studies reveal continuity in subsistence strategies throughout the sequence. This was based on specialisation in hunting red deer, fishing, and gathering molluscs, crustaceans and echinoderm species on rocky shores. The difference between the Azilian and Mesolithic occupations lies in the replacement of some marine invertebrate species and the decrease in limpet sizes. The abiotic evidence is characterised by a lithic assemblage with allochthonous flint types, which has been interpreted as continuity of the raw material procurement strategies inherited from the Magdalenian