8 research outputs found
Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall
Research Areas: Science & TechnologyABSTRACT - In this study, a rational combination of 200 pre-selected Carbohydrate-Active enzymes (CAZymes) and
sulfatases were tested, individually or combined, according to their ability to degrade Chlorella vulgaris
cell wall to access its valuable nutritional compounds. The disruption of microalgae cell walls by a four enzyme mixture (Mix) in comparison with the control, enabled to release up to 1.21g/L of reducing
sugars (p<0.001), led to an eight-fold increase in oligosaccharides release (p<0.001), and reduced
the fuorescence intensity by 47% after staining with Calcofuor White (p<0.001). The Mix treatment
was successful in releasing proteins (p<0.001), some MUFA (p<0.05), and the benefcial 18:3n-3 fatty
acid (p0.05), total carotenoids were
increased in the supernatant (p<0.05) from the Mix treatment, relative to the control. Taken together,
these results indicate that this four-enzyme Mix displays an efective capacity to degrade C. vulgaris cell
wall. Thus, these enzymes may constitute a good approach to improve the bioavailability of C. vulgaris
nutrients for monogastric diets, in particular, and to facilitate the cost-efective use of microalgae by
the feed industry, in general.info:eu-repo/semantics/publishedVersio
Impact of dietary incorporation of Spirulina (Arthrospira platensis) and exogenous enzymes on broiler performance, carcass traits and meat quality
This study assessed the effect of Spirulina
(Arthrospira platensis), individually and in combination
with exogenous enzymes, on growth
performance, carcass traits, and meat quality of broiler
chickens. One hundred and twenty Ross 308 male
chickens were allocated into 40 battery brooders, with 3
birds per cage, and fed ad libitum a corn-based diet
during the first 21 D of the trial. The experimental period
lasted from day 21 to 35, during which birds were fed 4
different diets: a corn-soybean basal diet, taken as the
control group, a basal diet containing 15% Spirulina
(MA), a basal diet containing 15% Spirulina plus 0.005%
Rovabio Excel AP (MAR), and a basal diet containing
15% Spirulina plus 0.01% lysozyme (MAL). Body weight
gain (P , 0.001) and feed conversion rate (P , 0.001)
were improved in control chickens, when compared with
those fed with Spirulina. In addition, Spirulina increased
the length of duodenum plus jejunum in relation to the
other treatment (P , 0.01). Chickens on the MAL diet showed a considerable increase in digesta viscosity
(P , 0.05) compared with the control group. Breast and
thigh meats from chickens fed with Spirulina, with or
without the addition of exogenous enzymes, had higher
values of yellowness (b*) (P , 0.001), total carotenoids
(P , 0.001), and saturated fatty acids (P , 0.001),
whereas n-3 polyunsaturated fatty acid (P , 0.01) and
a-tocopherol (P , 0.001) decreased, when compared
with the control. In conclusion, the incorporation of 15%
Spirulina in broiler diets, individually or combined with
exogenous enzymes, reduced birds’ performance through
a higher digesta viscosity, which is likely associated with
the gelation of microalga indigestible proteins. In addition,
cell wall of Spirulina was successfully broken by the
addition of lysozyme, but not by Rovabio Excel AP.
Therefore, we anticipate that the combination of lysozyme
with an exogenous specific peptidase could improve
the digestibility of proteins from this microalga and
avoid their detrimental gelationinfo:eu-repo/semantics/publishedVersio