An Appetite to Win: Disordered Eating Behaviours amongst Competitive Cyclists

Competitive cyclists may be vulnerable to disordered eating (DE) and eating disorders (ED) due to perceived body composition optimization and external influences within cycling culture and from stakeholders. Therefore, this study aimed to assess DE and ED risk in competitive cyclists using the Eating Attitudes Test (EAT-26), explore differences in responses based on sex, discipline, and level of competition, and to gain insights into contributing factors towards DE via open-ended survey questions. In total, 203 participants completed a mixed-method questionnaire. Eating disorders were reported by 5.7% (n = 11) of participants, with three being historic cases. The median (inter-quartile range) EAT-26 score was 8 (12) of a total possible score of 78. Disordered eating risk was observed in 16.7% of participants due to an EAT-26 score ≥20. Female participants had significantly higher scores than male participants (12.5 ± 17.5 vs. 6.5 ± 10.0; p = 0.004). There was no significant difference between road cyclists and off-road cyclists (7.0 ± 13.25 vs. 8.0 ± 10.5; p = 0.683). There was a significant difference in scores between novice/club/regional and national/elite/professional cyclists (6.0 ± 11.25 vs. 10.5 ± 12.0; p = 0.007). Thematic analysis of open-text responses found that the social environment of competitive cycling contributed towards DE behaviours and body image issues. These findings indicate competitive cyclists do appear to be an ‘at risk’ population for DE/ED. Therefore, there is need for stakeholders to enhance nutritional services, nutrition education, and create supportive athlete environments

Rapid identification of species, sex and maturity by mass spectrometric analysis of animal faeces

Background We describe a new approach to the recovery of information from faecal samples, based on the analysis of the molecular signature generated by rapid evaporative ionisation mass spectrometry (REIMS). Results Faecal pellets from five different rodent species were analysed by REIMS, and complex mass spectra were acquired rapidly (typically a few seconds per sample). The uninterpreted mass spectra (signatures) were then used to seed linear discriminant analysis and classification models based on random forests. It was possible to classify each species of origin with a high rate of accuracy, whether faeces were from animals maintained under standard laboratory conditions or wild-caught. REIMS signatures were stable to prior storage of the faecal material under a range of different conditions and were not altered rapidly or radically by changes in diet. Further, within species, REIMS signatures could be used to discriminate faeces from adult versus juvenile mice, male versus female mice and those from three different laboratory strains. Conclusions REIMS offers a completely novel method for the rapid analysis of faecal samples, extending faecal analysis (previously focused on DNA) to an assessment of phenotype, and has considerable potential as a new tool in the armamentarium of the field biologist

Structured Observations Reveal Slow HIV-1 CTL Escape

The existence of viral variants that escape from the selection pressures imposed by cytotox- ic T-lymphocytes (CTLs) in HIV-1 infection is well documented, but it is unclear when they arise, with reported measures of the time to escape in individuals ranging from days to years. A study of participants enrolled in the SPARTAC (Short Pulse Anti-Retroviral Thera- py at HIV Seroconversion) clinical trial allowed direct observation of the evolution of CTL es- cape variants in 125 adults with primary HIV-1 infection observed for up to three years. Patient HLA-type, longitudinal CD8+ T-cell responses measured by IFN- γ ELISpot and lon- gitudinal HIV-1 gag , pol , and nef sequence data were used to study the timing and preva- lence of CTL escape in the participants whilst untreated. Results showed that sequence variation within CTL epitopes at the first time point (within six months of the estimated date of seroconversion) was consistent with most mutations being transmitted in the infecting viral strain rather than with escape arising within the first few weeks of infection. Escape arose throughout the first three years of infection, but slowly and steadily. Approximately one third of patients did not drive any new escape in an HLA-restricted epitope in just under two years. Patients driving several escape mutations during these two years were rare and the median and modal numbers of new escape events in each patient were one and zero re- spectively. Survival analysis of time to escape found that possession of a protective HLA type significantly reduced time to first escape in a patient (p = 0.01), and epitopes escaped faster in the face of a measurable CD8+ ELISpot response (p = 0.001). However, even in an HLA matched host who mounted a measurable, specific, CD8+ response the average time before the targeted epitope evolved an escape mutation was longer than two year

Impact of antiretroviral therapy in primary HIV infection on natural killer cell function and the association with viral rebound and HIV DNA following treatment interruption

Natural Killer (NK) cells play a key role in controlling HIV replication, with potential downstream impact on the size of the HIV reservoir and likelihood of viral rebound after antiretroviral therapy (ART) cessation. It is therefore important to understand how primary HIV infection (PHI) disrupts NK cell function, and how these functions are restored by early ART. We examined the impact of commencing ART during PHI on phenotypic and functional NK cell markers at treatment initiation (baseline), 3 months, 1 year, and 2 years in seven well-characterised participants in comparison to HIV seronegative volunteers. We then examined how those NK cell properties differentially impacted by ART related to time to viral rebound and HIV DNA levels in 44 individuals from the SPARTAC trial who stopped ART after 48 weeks treatment, started during PHI. NK cell markers that were significantly different between the seven people with HIV (PWH) treated for 2 years and HIV uninfected individuals included NKG2C levels in CD56dim NK cells, Tim-3 expression in CD56bright NK cells, IFN-γ expressed by CD56dim NK cells after IL-12/IL-18 stimulation and the fraction of Eomes-/T-bet+ in CD56dim and CD56bright NK cells. When exploring time to viral rebound after stopping ART among the 44 SPARTAC participants, no single NK phenotypic marker correlated with control. Higher levels of IL-12/IL-18 mediated NK cell degranulation at baseline were associated with longer times to viral rebound after treatment interruption (P=0.028). Additionally, we found higher fractions of CD56dim NK cells in individuals with lower levels of HIV DNA (P=0.048). NKG2A and NKp30 levels in CD56neg NK cells were higher in patients with lower HIV DNA levels (p=0.00174, r=-0.49 and p=0.03, r= -0.327, respectively) while CD27 levels were higher in those with higher levels of HIV DNA (p=0.026). These data show NK cell functions are heterogeneously impacted by HIV infection with a mixed picture of resolution on ART, and that while NK cells may affect HIV DNA levels and time to viral rebound, no single NK cell marker defined delayed viral rebound

Seroprevalence of anti-SARS-CoV-2 IgG antibodies, risk factors for infection and associated symptoms in Geneva, Switzerland: a population-based study.

Aims: To assess SARS-CoV-2 seroprevalence over the first epidemic wave in the canton of Geneva, Switzerland, as well as risk factors for infection and symptoms associated with IgG seropositivity. Methods: Between April and June 2020, former participants of a representative survey of the 20-74-year-old population of canton Geneva were invited to participate in the study, along with household members aged over 5 years. Blood samples were tested for anti-SARS-CoV-2 immunoglobulin G. Questionnaires were self-administered. We estimated seroprevalence with a Bayesian model accounting for test performance and sampling design. Results: We included 8344 participants, with an overall adjusted seroprevalence of 7.8% (95% credible interval 6.8-8.9). Seroprevalence was highest among 18-49 year-olds (9.5%), and lowest in 5-9-year-old children (4.3%) and individuals >65 years (4.7-5.4%). Odds of seropositivity were significantly reduced for female retirees and unemployed men compared to employed individuals, and smokers compared to non-smokers. We found no significant association between occupation, level of education, neighborhood income and the risk of being seropositive. The symptom most strongly associated with seropositivity was anosmia/dysgeusia. Conclusions: Anti-SARS-CoV-2 population seroprevalence remained low after the first wave in Geneva. Socioeconomic factors were not associated with seropositivity in this sample. The elderly, young children and smokers were less frequently seropositive, although it is not clear how biology and behaviours shape these differences

HIV-1 DNA predicts disease progression and post-treatment virological control

In HIV-1 infection, a population of latently infected cells facilitates viral persistence despite antiretroviral therapy (ART). With the aim of identifying individuals in whom ART might induce a period of viraemic control on stopping therapy, we hypothesised that quantification of the pool of latently infected cells in primary HIV-1 infection (PHI) would predict clinical progression and viral replication following ART. We measured HIV-1 DNA in a highly characterised randomised population of individuals with PHI. We explored associations between HIV-1 DNA and immunological and virological markers of clinical progression, including viral rebound in those interrupting therapy. In multivariable analyses, HIV-1 DNA was more predictive of disease progression than plasma viral load and, at treatment interruption, predicted time to plasma virus rebound. HIV-1 DNA may help identify individuals who could safely interrupt ART in future HIV-1 eradication trials

Epistasis, core-genome disharmony, and adaptation in recombining bacteria

Recombination of short DNA fragments via horizontal gene transfer (HGT) can introduce beneficial alleles, create genomic disharmony through negative epistasis, and create adaptive gene combinations through positive epistasis. For non-core (accessory) genes, the negative epistatic cost is likely to be minimal because the incoming genes have not co-evolved with the recipient genome and are frequently observed as tightly linked cassettes with major effects. By contrast, interspecific recombination in the core genome is expected to be rare because disruptive allelic replacement is likely to introduce negative epistasis. Why then is homologous recombination common in the core of bacterial genomes? To understand this enigma, we take advantage of an exceptional model system, the common enteric pathogens Campylobacter jejuni and C. coli that are known for very high magnitude interspecies gene flow in the core genome. As expected, HGT does indeed disrupt co-adapted allele pairings, indirect evidence of negative epistasis. However, multiple HGT events enable recovery of the genome’s co-adaption between introgressing alleles, even in core metabolism genes (e.g., formate dehydrogenase). These findings demonstrate that, even for complex traits, genetic coalitions can be decoupled, transferred, and independently reinstated in a new genetic background—facilitating transition between fitness peaks. In this example, the two-step recombinational process is associated with C. coli that are adapted to the agricultural niche

Ernst Freund as Precursor of the Rational Study of Corporate Law

Gindis, David, Ernst Freund as Precursor of the Rational Study of Corporate Law (October 27, 2017). Journal of Institutional Economics, Forthcoming. Available at SSRN: https://ssrn.com/abstract=2905547, doi: https://dx.doi.org/10.2139/ssrn.2905547The rise of large business corporations in the late 19th century compelled many American observers to admit that the nature of the corporation had yet to be understood. Published in this context, Ernst Freund's little-known The Legal Nature of Corporations (1897) was an original attempt to come to terms with a new legal and economic reality. But it can also be described, to paraphrase Oliver Wendell Holmes, as the earliest example of the rational study of corporate law. The paper shows that Freund had the intuitions of an institutional economist, and engaged in what today would be called comparative institutional analysis. Remarkably, his argument that the corporate form secures property against insider defection and against outsiders anticipated recent work on entity shielding and capital lock-in, and can be read as an early contribution to what today would be called the theory of the firm.Peer reviewe

Assessing the materiality of nature-related financial risks for the UK

•At least half of global GDP is moderately or highly directly dependent on nature, and ultimately there is no economy without its critical services, including clean and abundant water, clean air and food. Nature across most of the globe has now been significantly altered by multiple human drivers, such as land-use change, pollution, extraction of minerals, abstraction of water and climate change. Statistics on the current state of biodiversity loss and environmental degradation are alarming: the extent and condition of ecosystems has declined in 50% of natural ecosystems, including more than 85% of wetland area lost, and 25% of species are at risk of extinction (IPBES, 2019). The 2019 Global Assessment Report of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) concluded that fourteen of the eighteen ecosystem services that were assessed had declined since the 1970s. The United Kingdom is no exception. The percentage of UK habitats ‘in favourable or improving conservation status’ has been deteriorating since 2007, exacerbating impacts on our soils, pollinators, air and environmental pollution, water and flood protection. Our analyses show that 75% of the United Kingdom is covered by at least one hotspot of natural capital depletion, and 25% is covered by two or more hotspots of natural capital depletion. The UK, with its globally interconnected economy, is also exposed to significant global emerging risks. •The erosion of UK and global natural capital generates significant and long-term risks to society and the UK economy and financial sector. Studies by Central Banks around the world have highlighted the high degree of dependence on nature and the exposure of financial portfolios to nature-related risks. What is not yet clear, is the extent to which this is a material risk to financial stability, on a par with other risks on the radar of Central Banks, and if so, on what timescales this risk could emerge and where it might ‘fall through the cracks’ of current supervision and regulation. •The objective of this project was to assess the materiality of nature-related risks to the UK financial sector both in the near-term and the longer-term. To address this, we develop six innovations: 1.The first Nature-Related Risk Inventory for the UK (UK-NRRI), equivalent in format to the National Risk Register or Climate Change Risk Assessment (CCRA). 2.Dependency analysis with spatial information to track dependencies on international supply and the nature risks therein, alongside transition risk exposures for the seven largest banks. 3.Development of sector-specific nature-related Value at Risk (nVaR) scores. 4.Co-development of three benchmark scenarios of nature-related risks, following the approach outlined by the Taskforce on Nature-Related Financial Disclosures (TNFD 2023). 5.Macroeconomic modelling to gauge the potential impacts on UK prosperity and resilience. 6.Preliminary financial ‘stress test’ for the domestic lending of the UK’s seven largest banks. The analyses focus on physical nature-related risks, with exposure analysis or transition risks. The findings demonstrate that biodiversity loss and environmental degradation create material risks for the UK economy and financial sector, in addition to their wider social and biodiversity impacts. These impacts are near and present. We find that the deterioration of our natural environment could slow economic growth and result in UK Gross Domestic Product (GDP) being 6% lower than it would have been otherwise by the 2030s under two scenarios (domestic and international) and 12% lower under an AMR-pandemic scenario. These are greater than the impact on GDP experienced in the Global Financial Crisis, in which UK GDP fell by around 4% to 6%, and - for the AMR-pandemic scenario – greater than the GDP impact of the COVID-19 pandemic when GDP fell 11% over 2020. While these findings are preliminary, all the evidence points to them being conservative. This study focussed on quantifying near- to medium-term risks, but the evidence clearly demonstrates these risks will increase over time with the potential for crossing tipping points. •The compounding impacts of climate and nature loss would have a very material impact on UK GDP; equivalent to several lost years of growth. It is not realistic to consider nature in isolation as climate change and environmental degradation are occurring in parallel and are interconnected. Environmental degradation increases the likelihood and severity of an acute climate or health shock, and the combined effect would have a very material impact on the economy. For this reason, we also draw upon the NGFS climate scenarios to explore the compounding impacts of climate and nature. We find that in our acute shock scenarios, these compounding impacts can lead to a UK GDP that is 8% lower than it would be otherwise, with a peak shock that wipes out around £200 billion from UK GDP and persists for several quarters, equivalent to 4 – 7 years of lost growth over the period. •The gradual impacts of environmental degradation on the economy are as detrimental or more so than climate change in the near-term - and the chronic year-to-year changes lead to losses that are as important as more sudden shocks. We find that the impact to GDP of chronic year-on-year environmental degradation is at least on par with that from physical climate change risks in the coming decade and for acute shocks, can be far greater than climate impacts alone. In effect, the impacts of environmental degradation are doubling or more the impact of climate change. Antimicrobial resistance (AMR) and zoonotic diseases are closely linked with deforestation and habitat destruction which can bring humans and wild animals into closer contact and have been shown to lead to greater abundance of antibiotic resistance genes in soil. Chronic nature-related risks associated with soil degradation, water provisioning, pollution and pollination services have material impacts on agriculture, manufacturing, construction, utilities and key supply chains. •Around half of UK nature-related risks come from overseas, through supply chains and financial exposures, pointing toward the importance of working internationally to close the gaps in disclosures and risk management. The four trillion GBP of financial assets assessed are dependent upon many trillions more of assets globally. Analyses of UK financial exposures suggest that 56% of the total upstream financial exposures have a High or Very High dependence on ecosystem services. Exposures to overseas risks are most material (in financial terms) for the services and manufacturing sectors, with highest risks related to water. The agricultural sector is most at risk in percentage terms, but the largest risks in monetary terms are to the services and manufacturing sectors. Agriculture can be impacted by disruption to several ecosystem services, with potential reductions in output (the nature-related value at risk – or nVaR) up to 15% of total annual production for disruption of any one service, albeit multiple ecosystem services at risk and impacts occur simultaneously, with risks to related to pollination services, soil quality and invasive species. Looking across the UK economy, we find that the nVaR associated with disruption to ecosystem services is in the hundreds of billions and equivalent to several percentage points of GDP; water risks alone are equivalent to around 13% UK GDP (for an extreme 1-in-100 year risk). The agricultural sector is also the most exposed to transition risks and opportunities. •Looking across the portfolios of the seven largest UK banks, the analyses indicate possible adjustments in the valuations of domestic holdings (excluding finance) of up to 4 – 5% over the coming decade from physical nature-related risks. Depending on the bank, the most at-risk sectors include agriculture, utilities, real-estate and manufacturing. Preliminary analyses suggest that between 8% and 53% of the portfolios of the seven largest banks are exposed also to transition risks. Firms could also derive opportunities from the nature-positive transition; including new demand for nature-positive products and services. The findings of this study take us further than previous studies to-date by clearly demonstrating the materiality of nature-related risks and the potential for compounding risks with climate change. Further work is needed to assess the implications for regulation, policy and supervision. Based upon the findings presented here and elsewhere, we believe there is a case for action by Central Banks, regulators and governments to assess if and where nature may be falling through the cracks of current frameworks and where this could lead to financial stability risks that justify explicit changes to those frameworks. We make a series of arguments based on the evidence for why nature may present new challenges that necessitate action. It is clear, for example, from other work (e.g. GARP 2024 and TNFD 2023), that there are sizeable information asymmetries created by the lack of disclosures of nature-related risks and impacts that mean that risks are currently under-priced. This may lead to an accumulation of systemic risk that goes undetected. We also lay out how nature-related risks suffer from the same ‘tragedy of the horizon’ issue identified by Mark Carney in 2015, but also additional challenges of a ‘tragedy of scale’ and unique drivers and risk transmission channels that are not captured within current climate-related risk assessments. •This study adds further evidence to support the conclusion from the Dasgupta Review and others that an early orderly transition toward a nature-positive economy brings significant benefits for UK prosperity and financial stability, through reducing both transition and physical risks, as well as for people and planet. Actions that could be considered by regulators and supervisors to mitigate risks to financial stability include advancing disclosures, broadening supervisory statements on climate to explicitly include environmental risks and introducing a simple nature-risk scenario within exploratory scenario exercises. There are many low-regrets measures that could be taken now, including supporting capability building through fora such as the Climate Financial Risk Forum and working with the scientific community to advance a set of benchmark scenarios, building upon those developed here. Regulators should also take timely opportunities to incorporate nature alongside climate into emerging frameworks, for example on transition plans, ISSB standards and taxonomies. •For financial institutions, the findings should motivate action to assess and manage nature-related financial risks, build capability, and begin to incorporate nature into emerging transition plans. These preliminary results suggest that even in the short-term nature-related risk is not negligible, especially if the losses are considered in relative terms to specific fractions of a lending portfolio. Financial institutions can manage risks to their own portfolios through working with their clients to reduce risks through supporting their transition and resilience. Importantly the transition toward a nature-positive economy presents opportunities as well as risks. With early action, UK firms – both financial and real-economy – can capture these opportunities. •For government, the materiality of nature-related risks demonstrated in this study add additional urgency to put in place the mechanisms, domestically and globally, to meet the goals and targets of the Kunming-Montreal Global Biodiversity Framework (GBF), as well as domestic policies such as the Environmental Improvement Plan (2023). This includes engaging internationally to ensure that emerging sustainable finance frameworks incorporate nature and nature related, including the IFRS Foundation’s ISSB. There is also an urgent rationale for investment and closer working with the scientific community to improve data and analytics as a public good to underpin the UK's transition to a resilient, nature-positive economy. •To continue to advance this area, strengthening collaboration between financial institutions and the scientific community is essential. Nature-related financial risk assessment is in its infancy, but risks are significant. This study has revealed several gaps in knowledge and approaches that require further study. It has also produced a series of tools that can be advanced today: •A Nature-Related Risk Inventory (UK-NRRI) that includes 29 key risks to the UK, with zoonotic diseases and antimicrobial resistance, soil health decline and global repercussions of food insecurity emerging as highest risks in terms of likelihood and impacts. •A set of benchmark narrative and quantitative scenarios that could form the basis for the further co-development of a set of scenarios for financial institutions, through fora such as the Climate Financial Risk Forum and UK Integrating Finance and Biodiversity network. There is a need to build platforms for collaboration and an opportunity to build upon existing fora such as the Climate Financial Risk Forum. The new £7m UKRI Integrating Finance and Biodiversity network, bringing together 17 research institutions, provides a ready venue to deepen collaborations. •Finally, while our focus has been on nature-related risks, the findings have implications for climate change given the strong interconnections between climate and nature The findings suggest that incorporating nature-related risk amplifications in climate scenarios would double the estimated impact of climate change on the UK economy, beyond what is currently predicted by the NGFS

Industry-Informed Workshops to Develop Graduate Skill Sets in the Circular Economy Using Systems Thinking

Increasing demand for chemicals worldwide, depleting resources, consumer pressure, stricter legislation, and the rising cost of waste disposal are placing increasing pressure on chemical and related industries. For any organization to survive in the current arena of growing climate change laws and regulations, and increasing public influence, the issue of sustainability must be fundamental to the way it operates. A sustainable manufacturing approach will enable economic growth to be combined with environmental and social sustainability and will be realized via collaboration between a multidisciplinary community including chemists, biologists, engineers, environmental scientists, economists, experts in management, and policy makers. Hence, employees with new skills, knowledge, and experience are essential. To realize this approach, the design and development of a series of workshops encompassing systems thinking are presented here. After close consultation with industry, an annual program of interactive workshops has been designed for graduate students to go beyond examining the "greening" of chemical reactions, processes, and products, and instead embed a systems thinking approach to learning. The workshops provide a valuable insight into the issues surrounding sustainable manufacturing covering change management, commercialization, environmental impact, circular economy, legislation, and bioresources incorporating the conversion of waste into valuable products. The multidisciplinary course content incorporates industrial case studies, providing access to real business issues, and is delivered by experts from academic departments across campus and industry