Feeding and management practices for racehorses in Turkey

The aim of this study was to gather information on feeding practices, and to identify nutrient imbalances of racehorses in Turkey. 134 racehorses who visited the TJK (Turkish Jockey Club) Racecourse Equine Hospital in Istanbul were referred for professional nutritional advice in 2013. Each horse was examined and body weight (BW), body condition score, exercise level, feeding practices, reasons for the veterinary visit as well as feed and supplement intake were recorded. Intakes of energy, starch, crude nutrients, and minerals were calculated and compared with NRC [15] recommendations. Descriptive data were calculated using commercial statistical software (IBM SPSS Statistics 24, Chicago, IL). The median age of the population was 3 years. The mean BW of the horses was 423.7 ± 38.4 kg and BCSwas 4.4/9 ± 0.7. Nearly all (99.2 %) horses were fed grass hay and 61 % of the horses received alfalfa as well. The average forage intake was 1.08 ± 0.42 % of BW/d. The average intake of concentrate was 1.2 ± 0.46 % of BW. Forty nine percent of the horses were fed supplements and only 12 % received oil. The mean DE intake was 1.03 ± 0.26 MJ DE/kg BW0.75 for thoroughbreds and 0.97 ± 0.19 MJ DE/kg BW0.75 for Arabians. The calculated mean starch intake was 5.0 ± 2.2 g/kg BW and the sugar intake was 1.2 ± 0.4 g/kg BW. Communication between horse owners/trainers and the equine nutritionists is needed with regard to formulating a proper diet for each horse

Integrating Traditional and Scientific Knowledge through Collaborative Natural Science Field Research: Identifying Elements for Success

We discuss two recent projects to examine the role of collaborative environmental fieldwork both in research and in the interactions between academically trained researchers and experienced local residents. The Bidarki Project studied black leather chitons (Katharina tunicata) in the lower Kenai Peninsula, Alaska. Its conclusion that chiton declines are part of a serial decline of intertidal invertebrates drew on collaborative fieldwork, archaeological data, historical records, and interviews with local residents. The Siku-Inuit-Hila Project studied sea ice in Barrow, Alaska; Clyde River, Nunavut; and Qaanaaq, Greenland. Quantitative data from locally maintained observation sites were supplemented by knowledge exchanges among hunters from the communities and by discussion in local working groups to develop an understanding of the physical dynamics and human uses of sea ice at each locale. We conclude that careful planning and preparation, along with the effort to build strong personal relationships, can increase the likelihood that collaborative fieldwork will be productive, enjoyable, and rewarding.Nous discutons de deux récents projets ayant eu pour but d’examiner le rôle d’études environnementales collaboratives sur le terrain, tant sur le plan de la recherche que sur le plan des interactions entre les chercheurs universitaires et les résidents expérimentés des localités visées. Le projet Bidarki se penchait sur les chitons noirs (Katharina tunicata) de la basse péninsule Kenai, en Alaska. La conclusion selon laquelle le déclin des chitons fait partie d’un déclin en série d’invertébrés intertidaux s’appuie sur des études collaboratives sur le terrain, sur des données archéologiques, sur des dossiers historiques ainsi que sur des entrevues de résidents des localités. Pour sa part, le projet Siku-Inuit-Hila a eu comme objectif d’étudier la glace de mer à Barrow, en Alaska; à Clyde River, au Nunavut; et à Qaanaaq, au Groenland. Les données quantitatives provenant de lieux d’observation entretenus localement ont été supplémentées par les échanges de connaissances des chasseurs des collectivités et par les discussions de groupes de travail locaux visant à mieux comprendre la dynamique physique et l’utilisation humaine de la glace de mer à chaque endroit. Nous en concluons que des travaux de planification et de préparation attentionnés, accompagnés d’efforts visant à nouer des liens personnels étroits, peuvent accroître la possibilité que les études collaboratives sur le terrain soient productives, agréables et valorisantes

Terminal NK cell maturation is controlled by concerted actions of T-bet and Zeb2 and is essential for melanoma rejection

Natural killer (NK) cell maturation is a tightly controlled process that endows NK cells with functional competence and the capacity to recognize target cells. Here, we found that the transcription factor (TF) Zeb2 was the most highly induced TF during NK cell maturation. Zeb2 is known to control epithelial to mesenchymal transition, but its role in immune cells is mostly undefined. Targeted deletion of Zeb2 resulted in impaired NK cell maturation, survival, and exit from the bone marrow. NK cell function was preserved, but mice lacking Zeb2 in NK cells were more susceptible to B16 melanoma lung metastases. Reciprocally, ectopic expression of Zeb2 resulted in a higher frequency of mature NK cells in all organs. Moreover, the immature phenotype of Zeb2(-/-) NK cells closely resembled that of Tbx21(-/-) NK cells. This was caused by both a dependence of Zeb2 expression on T-bet and a probable cooperation of these factors in gene regulation. Transgenic expression of Zeb2 in Tbx21(-/-) NK cells partially restored a normal maturation, establishing that timely induction of Zeb2 by T-bet is an essential event during NK cell differentiation. Finally, this novel transcriptional cascade could also operate in human as T-bet and Zeb2 are similarly regulated in mouse and human NK cells

It's about time: A synthesis of changing phenology in the Gulf of Maine ecosystem

© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Staudinger, M. D., Mills, K. E., Stamieszkin, K., Record, N. R., Hudak, C. A., Allyn, A., Diamond, A., Friedland, K. D., Golet, W., Henderson, M. E., Hernandez, C. M., Huntington, T. G., Ji, R., Johnson, C. L., Johnson, D. S., Jordaan, A., Kocik, J., Li, Y., Liebman, M., Nichols, O. C., Pendleton, D., Richards, R. A., Robben, T., Thomas, A. C., Walsh, H. J., & Yakola, K. It's about time: A synthesis of changing phenology in the Gulf of Maine ecosystem. Fisheries Oceanography, 28(5), (2019): 532-566, doi: 10.1111/fog.12429.The timing of recurring biological and seasonal environmental events is changing on a global scale relative to temperature and other climate drivers. This study considers the Gulf of Maine ecosystem, a region of high social and ecological importance in the Northwest Atlantic Ocean and synthesizes current knowledge of (a) key seasonal processes, patterns, and events; (b) direct evidence for shifts in timing; (c) implications of phenological responses for linked ecological‐human systems; and (d) potential phenology‐focused adaptation strategies and actions. Twenty studies demonstrated shifts in timing of regional marine organisms and seasonal environmental events. The most common response was earlier timing, observed in spring onset, spring and winter hydrology, zooplankton abundance, occurrence of several larval fishes, and diadromous fish migrations. Later timing was documented for fall onset, reproduction and fledging in Atlantic puffins, spring and fall phytoplankton blooms, and occurrence of additional larval fishes. Changes in event duration generally increased and were detected in zooplankton peak abundance, early life history periods of macro‐invertebrates, and lobster fishery landings. Reduced duration was observed in winter–spring ice‐affected stream flows. Two studies projected phenological changes, both finding diapause duration would decrease in zooplankton under future climate scenarios. Phenological responses were species‐specific and varied depending on the environmental driver, spatial, and temporal scales evaluated. Overall, a wide range of baseline phenology and relevant modeling studies exist, yet surprisingly few document long‐term shifts. Results reveal a need for increased emphasis on phenological shifts in the Gulf of Maine and identify opportunities for future research and consideration of phenological changes in adaptation efforts.This work was supported by the Department of the Interior Northeast Climate Adaptation Science Center (G14AC00441) for MDS, AJ, and KY; the National Science Foundation's Coastal SEES Program (OCE‐1325484) for KEM, ACT, MEH, and AA; the National Aeronautics and Space Administration (NNX16 AG59G) for ACT, KEM, NRR, and KSS; the USGS Climate Research and Development Program for TGH; National Science & Engineering Research Council of Canada, University of New Brunswick, Environment Canada, Sir James Dunn Wildlife Research Centre, and New Brunswick Wildlife Trust Fund for AD. We also thank the Regional Association for Research on the Gulf of Maine for support, and the Gulf of Maine Research Institute for hosting and providing in kind resources for a two day in‐person workshop in August 2016. We greatly appreciate contributions from K. Alexander, G. Calandrino, C. Feurt, I. Mlsna, N. Rebuck, J. Seavey, and J. Sun for helping shape the initial scope of the manuscript. We thank J. Weltzin and two anonymous reviewers for their constructive comments. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the views of the Northeast Climate Adaptation Science Center, U.S. Geological Survey, National Oceanographic and Atmospheric Administration, Fisheries and Oceans Canada or the US Environmental Protection Agency. This manuscript is submitted for publication with the understanding that the United States Government is authorized to reproduce and distribute reprints for Governmental purposes. None of the authors have conflicts of interest to declare in association with the contents of this manuscript

Pratiques documentaires numériques à l'université

Cet ouvrage, coordonné par Ghislaine Chartron, professeur au conservatoire des Arts et métiers (CNAM) et responsable de l'Institut national des techniques de la documentation (INTD). Annaïg Mahé, maître de conférences à l'Unité régionale de formation à l'information scientifique et technique (Urfist) de Paris et à l'école des Chartes, et Benoît Epron, maître de conférences à l'enssib, se propose d'explorer les pratiques documentaires dans l'enseignement supérieur et la recherche au moment clé du développement, voire de la croissance exponentielle de l'utilisation des outils numériques. À l'heure d'une maturité croissante de l'offre documentaire numérique et de ses potentiels dans tous les champs scientifiques, que connaissons-nous vraiment de la réalité des pratiques documentaires à l'université et dans les organismes de recherche ? Faire le point sur cette question fut l'objectif de la journée d'étude « Diversité des pratiques documentaires numériques dans les champs scientifiques » organisée à l'enssib en juillet 2009, qui a souhaité réunir un ensemble de travaux récents couvrant une large palette d'observations des pratiques dans la recherche et l'enseignement universitaire. Cette diversité concerne les méthodologies convoquées (quantitatives et qualitatives), les différents supports (notamment revues et ouvrages) et les différents champs disciplinaires (physique des hautes énergies, mathématiques et informatique, sciences de l'éducation, sciences politiques) : des mises en perspective plus transversales sont par ailleurs complémentaires. Les points de vue abordés sont internationaux

Intracellular Serine Protease Inhibitor SERPINB4 Inhibits Granzyme M-Induced Cell Death

Granzyme-mediated cell death is the major pathway for cytotoxic lymphocytes to kill virus-infected and tumor cells. In humans, five different granzymes (i.e. GrA, GrB, GrH, GrK, and GrM) are known that all induce cell death. Expression of intracellular serine protease inhibitors (serpins) is one of the mechanisms by which tumor cells evade cytotoxic lymphocyte-mediated killing. Intracellular expression of SERPINB9 by tumor cells renders them resistant to GrB-induced apoptosis. In contrast to GrB, however, no physiological intracellular inhibitors are known for the other four human granzymes. In the present study, we show that SERPINB4 formed a typical serpin-protease SDS-stable complex with both recombinant and native human GrM. Mutation of the P2-P1-P1′ triplet in the SERPINB4 reactive center loop completely abolished complex formation with GrM and N-terminal sequencing revealed that GrM cleaves SERPINB4 after P1-Leu. SERPINB4 inhibited GrM activity with a stoichiometry of inhibition of 1.6 and an apparent second order rate constant of 1.3×104 M−1s−1. SERPINB4 abolished cleavage of the macromolecular GrM substrates α-tubulin and nucleophosmin. Overexpression of SERPINB4 in tumor cells inhibited recombinant GrM-induced as well as NK cell-mediated cell death and this inhibition depended on the reactive center loop of the serpin. As SERPINB4 is highly expressed by squamous cell carcinomas, our results may represent a novel mechanism by which these tumor cells evade cytotoxic lymphocyte-induced GrM-mediated cell death

An international effort towards developing standards for best practices in analysis, interpretation and reporting of clinical genome sequencing results in the CLARITY Challenge

There is tremendous potential for genome sequencing to improve clinical diagnosis and care once it becomes routinely accessible, but this will require formalizing research methods into clinical best practices in the areas of sequence data generation, analysis, interpretation and reporting. The CLARITY Challenge was designed to spur convergence in methods for diagnosing genetic disease starting from clinical case history and genome sequencing data. DNA samples were obtained from three families with heritable genetic disorders and genomic sequence data were donated by sequencing platform vendors. The challenge was to analyze and interpret these data with the goals of identifying disease-causing variants and reporting the findings in a clinically useful format. Participating contestant groups were solicited broadly, and an independent panel of judges evaluated their performance. RESULTS: A total of 30 international groups were engaged. The entries reveal a general convergence of practices on most elements of the analysis and interpretation process. However, even given this commonality of approach, only two groups identified the consensus candidate variants in all disease cases, demonstrating a need for consistent fine-tuning of the generally accepted methods. There was greater diversity of the final clinical report content and in the patient consenting process, demonstrating that these areas require additional exploration and standardization. CONCLUSIONS: The CLARITY Challenge provides a comprehensive assessment of current practices for using genome sequencing to diagnose and report genetic diseases. There is remarkable convergence in bioinformatic techniques, but medical interpretation and reporting are areas that require further development by many groups