Transcriptome Analysis Describing New Immunity and Defense Genes in Peripheral Blood Mononuclear Cells of Rheumatoid Arthritis Patients

Background: Large-scale gene expression profiling of peripheral blood mononuclear cells from Rheumatoid Arthritis (RA) patients could provide a molecular description that reflects the contribution of diverse cellular responses associated with this disease. The aim of our study was to identify peripheral blood gene expression profiles for RA patients, using Illumina technology, to gain insights into RA molecular mechanisms. Methodology/Principal Findings: The Illumina Human-6v2 Expression BeadChips were used for a complete genome-wide transcript profiling of peripheral blood mononuclear cells (PBMCs) from 18 RA patients and 15 controls. Differential analysis per gene was performed with one-way analysis of variance (ANOVA) and P values were adjusted to control the False Discovery Rate (FDR < 5%). Genes differentially expressed at significant level between patients and controls were analyzed using Gene Ontology (GO) in the PANTHER database to identify biological processes. A differentially expression of 339 Reference Sequence genes (238 down-regulated and 101 up-regulated) between the two groups was observed. We identified a remarkably elevated expression of a spectrum of genes involved in Immunity and Defense in PBMCs of RA patients compared to controls. This result is confirmed by GO analysis, suggesting that these genes could be activated systemically in RA. No significant down-regulated ontology groups were found. Microarray data were validated by real time PCR in a set of nine genes showing a high degree of correlation. Conclusions/Significance: Our study highlighted several new genes that could contribute in the identification of innovative clinical biomarkers for diagnostic procedures and therapeutic interventions

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Brain atrophy after cortical hyperintensities in systemic lupus erythematosus

Universidade Federal de São Paulo, Departamento de Neurologia e Neurocirurgia, Divisão Geral de Neurologia, Sao Paulo SP, BrazilUniversidade Federal de São Paulo, Departamento de Neurologia e Neurocirurgia, Divisão Geral de Neurologia, Sao Paulo SP, BrazilWeb of Scienc

The Ig VH complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor

The present work aims at investigating the mechanism of action of the Rb9 peptide, which contains the VHCDR 3 sequence of anti-sodium-dependent phosphate transport protein 2B (NaPi2B) monoclonal antibody RebMab200 and displayed antitumor properties. Short peptides corresponding to the hypervariable complementarity-determining regions (CDRs) of immunoglobulins have been associated with antimicrobial, antiviral, immunomodulatory and antitumor activities regardless of the specificity of the antibody. We have shown that the CDR derived peptide Rb9 induced substrate hyperadherence, inhibition of cell migration and matrix invasion in melanoma and other tumor cell lines. Rb9 also inhibited metastasis of murine melanoma in a syngeneic mouse model. We found that Rb9 binds to and interferes with Hsp90 chaperone activity causing attenuation of FAK-Src signaling and downregulation of active Rac1 in B16F10-Nex2 melanoma cells. The peptide also bound to an adhesion G-protein coupled receptor, triggering a concentration-dependent synthesis of cAMP and activation of PKA and VASP signaling as well as IP-3 dependent Ca2+ release. Hsp90 is highly expressed on the cell surface of melanoma cells, and synthetic agents that target Hsp90 are promising cancer therapeutic drugs. Based on their remarkable antitumor effects, the CDR-H3-derived peptides from RebMab200, and particularly the highly soluble and stable Rb9, are novel candidates to be further studied as potential antitumor drugs, selectively acting on cancer cell motility and invasion

Genetic progress of phenotypic recurrent selection in popcorn Progresso genético da seleção recorrente fenotípica em milho-pipoca

Popcorn is widely consumed in the south of Minas Gerais; however, up to now no specific breeding program for the region has been carried out. Recurrent selection (RS) is a viable alternative for breeding of this crop; nevertheless, it is necessary to frequently evaluate the progress achieved. Consequently, the purpose of this work was to assess the genetic progress obtained after two cycles of RS for the traits of popping expansion (PE) and grain yield (GY). Cycles one and two of the UFLA - E and UFLA - R populations, the original population (UFLA) and two controls were assessed. The experiment was conducted at the UFLA experimental farm in a randomized block design with eleven replications; ten agronomic traits, including PE and GY, were assessed. Gains from selection for the traits studied were observed, as well as that the type of kernel had an influence on the response for genetic progress for the PE trait and that the populations assessed exhibited potential for future selection cycles.<br>O milho-pipoca é amplamente consumido no Sul de Minas Gerais, entretanto, até o momento, nenhum programa de melhoramento especifico para a região foi conduzido. A seleção recorrente (RS) é uma alternativa viável para o melhoramento da cultura, todavia faz-se necessário verificar frequentemente o progresso obtido. Por conseguinte, objetivou-se avaliar o progresso genético obtido após dois ciclos de RS para os caracteres capacidade de expansão (PE) e rendimento de grãos (GY). Foram avaliados os ciclos um e dois das populações UFLA - E e UFLA - R, a população original (UFLA) e duas testemunhas. O experimento foi conduzido na fazenda experimental da UFLA, no delineamento em blocos ao acaso com onze repetições, dez características agronômicas incluindo PE e GY foram avaliadas. Constataram-se ganhos com a seleção para os caracteres estudados, que o tipo de grão (pérola e espinho) influenciou na resposta do progresso genético para o caráter PE e que as populações avaliadas apresentam potencial para futuros ciclos seletivo