New challenges for Adaptive Optics: Extremely Large Telescopes

The performance of an adaptive optics (AO) system on a 100m diameter ground based telescope working in the visible range of the spectrum is computed using an analytical approach. The target Strehl ratio of 60% is achieved at 0.5um with a limiting magnitude of the AO guide source near R~10, at the cost of an extremely low sky coverage. To alleviate this problem, the concept of tomographic wavefront sensing in a wider field of view using either natural guide stars (NGS) or laser guide stars (LGS) is investigated. These methods use 3 or 4 reference sources and up to 3 deformable mirrors, which increase up to 8-fold the corrected field size (up to 60\arcsec at 0.5 um). Operation with multiple NGS is limited to the infrared (in the J band this approach yields a sky coverage of 50% with a Strehl ratio of 0.2). The option of open-loop wavefront correction in the visible using several bright NGS is discussed. The LGS approach involves the use of a faint (R ~22) NGS for low-order correction, which results in a sky coverage of 40% at the Galactic poles in the visible.Comment: 11 pages, 9 figures, 4 tables. Accepted for publication in MNRA

Antifungal activity of novel microcycle derivatives

Cryptococcus neoformans is an opportunistic fungal pathogen that causes cryptococcosis. After inhalation, the organism disseminates to the brain, where it causes cryptococcal meningitis. Annually, approximately 225,000 immunocompromised individuals develop cryptococcal meningitis, resulting in over 181,000 deaths. To treat these patients, there are only four classes of antifungals currently available and these options are toxic at high concentrations and ineffective. In addition, fungal pathogens are becoming resistant to existing antifungals. In the current study, we are testing the antifungal activity of macrocycle compounds against C. neoformans. These compounds have been shown to be active against many other fungal pathogens, allowing us to hypothesize that these compounds would exhibit antifungal activity against C. neoformans. We first tested 12 macrocycle compounds against C. neoformans strain H99. After incubating each compound at different concentrations with H99, the minimum inhibitory concentration (MIC) was calculated. Compounds exhibiting antifungal activity were then tested for cytotoxicity using the mouse macrophage cell line J774.A. Effective, non-toxic compounds were then assayed with existing antifungal drugs in checkerboard assays to determine possible synergistic or antagonistic activity. The majority of the compounds showed antifungal activity. Of these compounds, 6 were non-toxic. Initial checkerboard assays have shown synergistic and indifferent interactions between the tested compounds and antifungal drugs. Future studies will focus on identifying the mechanism of action of these compounds. Confocal and electron microscopy will be used to identify changes in fungal cell wall & membrane morphology, and screening of mutant libraries will be used to identify mutants resistant to these compounds.Lew Wentz FoundationMicrobiology and Molecular GeneticsChemistryPhysic

Synthesis of a [2]rotaxane through first- and second-sphere coordination

In an effort to expand the application of a new template from interpenetrated to interlocked molecular species, we report the synthesis of a new [2]rotaxane by means of both first- and second-sphere coordination of a palladium(II) dichloride subunit

SINFONI in the Galactic Center: young stars and IR flares in the central light month

We report 75 milli-arcsec resolution, near-IR imaging spectroscopy within the central 30 light days of the Galactic Center [...]. To a limiting magnitude of K~16, 9 of 10 stars in the central 0.4 arcsec, and 13 of 17 stars out to 0.7 arcsec from the central black hole have spectral properties of B0-B9, main sequence stars. [...] all brighter early type stars have normal rotation velocities, similar to solar neighborhood stars. We [...] derive improved 3d stellar orbits for six of these S-stars in the central 0.5 arcsec. Their orientations in space appear random. Their orbital planes are not co-aligned with those of the two disks of massive young stars 1-10 arcsec from SgrA*. We can thus exclude [...] that the S-stars as a group inhabit the inner regions of these disks. They also cannot have been located/formed in these disks [...]. [...] we conclude that the S-stars were most likely brought into the central light month by strong individual scattering events. The updated estimate of distance to the Galactic center from the S2 orbit fit is Ro = 7.62 +/- 0.32 kpc, resulting in a central mass value of 3.61 +/- 0.32 x 10^6 Msun. We happened to catch two smaller flaring events from SgrA* [...]. The 1.7-2.45 mum spectral energy distributions of these flares are fit by a featureless, red power law [...]. The observed spectral slope is in good agreement with synchrotron models in which the infrared emission comes from [...] radiative inefficient accretion flow in the central R~10 Rs region.Comment: 50 pages, 10 figures, 2 tables, submitted to ApJ, February 6th, 2005, abstract abridge

Isobutyl acetate: electronic state spectroscopy by high-resolution vacuum ultraviolet photoabsorption, He(I) photoelectron spectroscopy and ab initio calculations

The high-resolution vacuum ultraviolet photoabsorption spectrum of isobutyl acetate, C6H12O2, is presented here and was measured over the energy range 4.3–10.8 eV (290–115 nm). Valence and Rydberg transitions with their associated vibronic series have been observed in the photoabsorption spectrum and are assigned in accordance with new ab initio calculations of the vertical excitation energies and oscillator strengths. The measured photoabsorption cross sections have been used to calculate the photolysis lifetime of this ester in the Earth’s upper atmosphere (20–50 km). Calculations have also been carried out to determine the ionization energies and fine structure of the lowest ionic state of isobutyl acetate and are compared with a photoelectron spectrum (from 9.5 to 16.7 eV), recorded for the first time. Vibrational structure is observed in the first photoelectron band of this molecule

Adaptive Optics for Astronomy

Adaptive Optics is a prime example of how progress in observational astronomy can be driven by technological developments. At many observatories it is now considered to be part of a standard instrumentation suite, enabling ground-based telescopes to reach the diffraction limit and thus providing spatial resolution superior to that achievable from space with current or planned satellites. In this review we consider adaptive optics from the astrophysical perspective. We show that adaptive optics has led to important advances in our understanding of a multitude of astrophysical processes, and describe how the requirements from science applications are now driving the development of the next generation of novel adaptive optics techniques.Comment: to appear in ARA&A vol 50, 201

Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA

Gene expression is highly regulated at the step of transcription initiation, and transcription activators play a critical role in this process. RbpA, an actinobacterial transcription activator that is essential in Mycobacterium tuberculosis (Mtb), binds selectively to group 1 and certain group 2 σ-factors. To delineate the molecular mechanism of RbpA, we show that the Mtb RbpA σ-interacting domain (SID) and basic linker are sufficient for transcription activation. We also present the crystal structure of the Mtb RbpA-SID in complex with domain 2 of the housekeeping σ-factor, σ(A). The structure explains the basis of σ-selectivity by RbpA, showing that RbpA interacts with conserved regions of σ(A) as well as the nonconserved region (NCR), which is present only in housekeeping σ-factors. Thus, the structure is the first, to our knowledge, to show a protein interacting with the NCR of a σ-factor. We confirm the basis of selectivity and the observed interactions using mutagenesis and functional studies. In addition, the structure allows for a model of the RbpA-SID in the context of a transcription initiation complex. Unexpectedly, the structural modeling suggests that RbpA contacts the promoter DNA, and we present in vivo and in vitro studies supporting this finding. Our combined data lead to a better understanding of the mechanism of RbpA function as a transcription activator

Transition Metal Complexes of Dibenzyl Tetraazamacrocycles

Tetraazamacrocycles, cyclic molecules with four nitrogen atoms, have long been known to produce highly stable transition metal complexes. Cross-bridging such molecules with 2-carbon chains has been shown to enhance the stability of these complexes even further, providing enough stability to use the resulting compounds in applications as diverse and demanding as aqueous, green oxidation catalysis all the way to drug molecules injected into humans. Although the stability of these compounds is believed to result from the increased rigidity and topological complexity imparted by the cross-bridge, there is insufficient experimental data to exclude other causes. In this study, standard organic and inorganic synthetic methods were used to produce unbridged dibenzyl tetraazamacrocycle analogues of known cross-bridged tetraazamacrocycles and their transition metal complexes to allow direct comparison of molecules identical except for the cross-bridge. The syntheses of the known tetraazamacrocycles and the novel transition metal complexes were successful with high yields and purity. Initial chemical characterization of the complexes by UV-Visible spectroscopy and cyclic voltammetry shows little difference in electronic properties from bridged versions. Direct comparison studies of the unbridged and bridged compounds’ stabilities remain to be carried out and will shed light on the importance of the cross-bridge to complex robustness