Impact of helium diffusion and helium-flash-induced carbon production on gravity-mode pulsations in subdwarf B stars

Realistic stellar models are essential to the forward modelling approach in asteroseismology. For practicality however, certain model assumptions are also required. For example, in the case of subdwarf B stars, one usually starts with zero-age horizontal branch structures without following the progenitor evolution. We analyse the effects of common assumptions in subdwarf B models on the g-mode pulsational properties. We investigate if and how the pulsation periods are affected by the H-profile in the core-envelope transition zone. Furthermore, the effects of C-production and convective mixing during the core helium flash are evaluated. Finally, we reanalyse the effects of stellar opacities on the mode excitation in subdwarf B stars. We find that helium settling causes a shift in the theoretical blue edge of the g-mode instability domain to higher effective temperatures. This results in a closer match to the observed instability strip of long-period sdB pulsators, particularly for l<=3 modes. We show further that the g-mode spectrum is extremely sensitive to the H-profile in the core-envelope transition zone. If atomic diffusion is efficient, details of the initial shape of the profile become less important in the course of evolution. Diffusion broadens the chemical gradients, and results in less effective mode trapping and different pulsation periods. Furthermore, we report on the possible consequences of the He-flash for the g-modes. The outer edge of a flash-induced convective region introduces an additional chemical transition in the stellar models, and the corresponding spike in the Brunt-Vaisala frequency produces a complicated mode trapping signature in the period spacings.Comment: 9 pages, 6 figures, 1 table, accepted for publication in A&

The blue-edge problem of the V1093 Her instability strip revisited using evolutionary models with atomic diffusion

We have computed a new grid of evolutionary subdwarf B star (sdB) models from the start of central He burning, taking into account atomic diffusion due to radiative levitation, gravitational settling, concentration diffusion, and thermal diffusion. We have computed the non-adiabatic pulsation properties of the models and present the predicted p-mode and g-mode instability strips. In previous studies of the sdB instability strips, artificial abundance enhancements of Fe and Ni were introduced in the pulsation driving layers. In our models, the abundance enhancements of Fe and Ni occur naturally, eradicating the need to use artificial enhancements. We find that the abundance increases of Fe and Ni were previously underestimated and show that the instability strip predicted by our simulations solves the so-called blue edge problem of the subdwarf B star g-mode instability strip. The hottest known g-mode pulsator, KIC 10139564, now resides well within the instability strip {even when only modes with low spherical degrees (l<=2) are considered.Comment: 7 pages, 7 figures. Accepted for publication in Astronomy & Astrophysic

Induction of CCL8/MCP-2 by mycobacteria through the activation of TLR2/PI3K/Akt signaling pathway.

Pleural tuberculosis (TB), together with lymphatic TB, constitutes more than half of all extrapulmonary cases. Pleural effusions (PEs) in TB are representative of lymphocytic PEs which are dominated by T cells. However, the mechanism underlying T lymphocytes homing and accumulation in PEs is still incompletely understood. Here we performed a comparative analysis of cytokine abundance in PEs from TB patients and non-TB patients by protein array analysis and observed that MCP-2/CCL8 is highly expressed in the TB-PEs as compared to peripheral blood. Meanwhile, we observed that CCR5, the primary receptor used by MCP-2/CCL8, is mostly expressed on pleural CD4(+) T lymphocytes. Furthermore, we found that infection with either Mycobacterium bovis Bacillus Calmette-Guérin (BCG) or Mycobacterium tuberculosis H37Rv induced production of MCP-2/CCL8 at both transcriptional and protein level in Raw264.7 and THP-1 macrophage cells, mouse peritoneal macrophages as well as human PBMC monocyte-derived macrophages (MDMs). The induction of MCP-2/CCL8 by mycobacteria is dependent on the activation of TLR2/PI3K/Akt and p38 signaling pathway. We conclude that accumulation of MCP-2/CCL8 in TB-PEs may function as a biomarker for TB diagnosis

Slowing down atomic diffusion in subdwarf B stars: mass loss or turbulence?

Subdwarf B stars show chemical peculiarities that cannot be explained by diffusion theory alone. Both mass loss and turbulence have been invoked to slow down atomic diffusion in order to match observed abundances. The fact that some sdB stars show pulsations gives upper limits on the amount of mass loss and turbulent mixing allowed. Consequently, non-adiabatic asteroseismology has the potential to decide which process is responsible for the abundance anomalies. We compute for the first time seismic properties of sdB models with atomic diffusion included consistently during the stellar evolution. The diffusion equations with radiative forces are solved for H, He, C, N, O, Ne, Mg, Fe and Ni. We examine the effects of various mass-loss rates and mixed surface masses on the abundances and mode stability. It is shown that the mass-loss rates needed to simulate the observed He abundances (10^{-14}<=Mdot [Msun/yr]<=10^{-13}) are not consistent with observed pulsations. We find that for pulsations to be driven the rates should be Mdot<=10^{-15} Msun/yr. On the other hand, weak turbulent mixing of the outer 10^{-6} Msun can explain the He abundance anomalies while still allowing pulsations to be driven. The origin of the turbulence remains unknown but the presence of pulsations gives tight constraints on the underlying turbulence model.Comment: 12 pages, 8 figures, 1 table, accepted for publication in MNRA

Proteomic analysis of spinal cord tissue in a rat model of cancer-induced bone pain

BackgroundCancer-induced bone pain (CIBP) is a moderate to severe pain and seriously affects patients’ quality of life. Spinal cord plays critical roles in pain generation and maintenance. Identifying differentially expressed proteins (DEPs) in spinal cord is essential to elucidate the mechanisms of cancer pain.MethodsCIBP rat model was established by the intratibial inoculation of MRMT-1 cells. Positron emission tomography (PET) scan and transmission electron microscopy (TEM) were used to measure the stats of spinal cord in rats. Label free Liquid Chromatography with tandem mass spectrometry (LC-MS-MS) were used to analyze the whole proteins from the lumbar spinal cord. Differentially expressed proteins (DEPs) were performed using Gene Ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis, and verified using Western blot and immunofluorescence assay.ResultsIn the current study, CIBP rats exhibited bone damage, spontaneous pain, mechanical hyperalgesia, and impaired motor ability. In spinal cord, an hypermetabolism and functional abnormality were revealed on CIBP rats. An increase of synaptic vesicles density in active zone and a disruption of mitochondrial structure in spinal cord of CIBP rats were observed. Meanwhile, 422 DEPs, consisting of 167 up-regulated and 255 down-regulated proteins, were identified among total 1539 proteins. GO enrichment analysis indicated that the DEPs were mainly involved in catabolic process, synaptic function, and enzymic activity. KEGG pathway enrichment analysis indicated a series of pathways, including nervous system disease, hormonal signaling pathways and amino acid metabolism, were involved. Expression change of synaptic and mitochondrial related protein, such as complexin 1 (CPLX1), synaptosomal-associated protein 25 (SNAP25), synaptotagmin 1 (SYT1), aldehyde dehydrogenase isoform 1B1 (ALDH1B1), Glycine amidinotransferase (GATM) and NADH:ubiquinone oxidoreductase subunit A11 (NDUFA11), were further validated using immunofluorescence and Western blot analysis.ConclusionThis study provides valuable information for understanding the mechanisms of CIBP, and supplies potential therapeutic targets for cancer pain

Gravitational settling in pulsating subdwarf B stars and their progenitors

Diffusion of atoms can be important during quiescent phases of stellar evolution. Particularly in the very thin inert envelopes of subdwarf B stars, diffusive movements will considerably change the envelope structure and the surface abundances on a short timescale. Also, the subdwarfs will inherit the effects of diffusion in their direct progenitors, namely giants near the tip of the red giant branch. This will influence the global evolution and the pulsational properties of subdwarf B stars. We investigate the impact of gravitational settling, thermal diffusion and concentration diffusion on the evolution and pulsations of subdwarf B stars. Our diffusive stellar models are compared with models evolved without diffusion. We constructed subdwarf B models with a mass of 0.465 Msun from a 1 and 3 Msun ZAMS progenitor. The low mass star ignited helium in an energetic flash, while the intermediate mass star started helium fusion gently. For each progenitor type we computed series with and without atomic diffusion. Atomic diffusion in red giants causes the helium core mass at the onset of helium ignition to be larger. We find an increase of 0.0015 Msun for the 1 Msun model and 0.0036 Msun for the 3 Msun model. The effects on the red giant surface abundances are small after the first dredge up. The evolutionary tracks of the diffusive subdwarf B models are shifted to lower surface gravities and effective temperatures due to outward diffusion of hydrogen. This affects both the frequencies of the excited modes and the overall frequency spectrum. Especially the structure and pulsations of the post-non-degenerate sdB star are drastically altered, proving that atomic diffusion cannot be ignored in these stars.Comment: 10 pages, 6 figures, accepted for publication in A&

Impacts of the Degraded Alpine Swamp Meadow on Tensile Strength of Riverbank: A Case Study of the Upper Yellow River

In the meandering riverbank of the Upper Yellow River (UYR), the native alpine swamp meadow (AS) has continuously degenerated into an alpine meadow (AM) due to climate change and intensified grazing. Its implication on river morphology is still not well known. This study examined this effect by in situ measurings of (1) physical properties of roots and their distribution in the soil-root mixture of the upper bank layer, and (2) the tensile strength in terms of excavating tests for triggering cantilever collapses of AS and AM riverbanks. The results showed that the root number in AS was significantly greater than that in AM, though the root distribution in both was similar. Also, the average tensile strength of individual roots in AS was 31,310 kPa, while that in AM was only 16,155 kPa. For the soil-root mixture, it decreased from 67.39 to 21.96 kPa. The weakened mechanical property was mainly ascribed to the lessened root number and the simpler root structure in the soil-root mixture of AM that reduces its ability to resist the external force. These findings confirmed that healthy AS can enhance bank stability and delay the development of tensile cracks in the riverbank of the meandering rivers in the UYR

Privacy-preserving local analysis of digital trace data: A proof-of-concept

We present PORT, a software platform for local data extraction and analysis of digital trace data. While digital trace data hold huge potential for social-scientific discovery, their most useful parts have been unattainable for scientists because of privacy concerns and prohibitive access to application programming interfaces. Recently, a workflow was introduced allowing citizens to donate their digital traces to scientists. In this workflow, citizens’ digital traces are processed locally on their machines before providing informed consent to share a subset of the data with researchers. In this paper, we present the newly developed software PORT that implements the local processing part of this workflow, protecting privacy by shielding sensitive data from outside observers, including the researchers themselves. When using PORT, researchers can tailor the local processing procedure suitable to the data download package and research question. Thus, PORT enables a host of potential applications of social data science to hitherto unobtainable data

An evolutionary study of the pulsating subdwarf B eclipsing binary PG1336-018 (NY Vir)

The formation of subdwarf B (sdB) stars is not well understood within the current framework of stellar single and binary evolution. In this study, we focus on the formation and evolution of the pulsating sdB star in the very short-period eclipsing binary PG1336-018. We aim at refining the formation scenario of this unique system, so that it can be confronted with observations. We probe the stellar structure of the progenitors of sdB stars in short-period binaries using detailed stellar evolution calculations. Applying this to PG1336-018 we reconstruct the common-envelope phase during which the sdB star was formed. The results are interpreted in terms of the standard common-envelope formalism (the alpha-formalism) based on the energy equation, and an alternative description (the gamma-formalism) using the angular momentum equation. We find that if the common-envelope evolution is described by the alpha-formalism, the sdB progenitor most likely experienced a helium flash. We then expect the sdB mass to be between 0.39 and 0.48 Msun, and the sdB progenitor initial mass to be below ~2 Msun. However, the results for the gamma-formalism are less restrictive, and a broader sdB mass range (0.3 - 0.8 Msun) is possible in this case. Future seismic mass determination will give strong constraints on the formation of PG1336-018 and, in particular, on the CE phase.Comment: 9 pages, 7 figures, 2 tables, accepted for publication in A&