Welding high-strength aluminum alloys

Handbook has been published which integrates results of 19 research programs involving welding of high-strength aluminum alloys. Book introduces metallurgy and properties of aluminum alloys by discussing commercial alloys and heat treatments. Several current welding processes are reviewed such as gas tungsten-arc welding and gas metal-arc welding

Leaders for North Carolina's schools : a review of programs for the professional development of principals

The purpose of this study was to describe and evaluate four programs North Carolina has implemented for the professioanl development of principals. The programs included the Principals' Executive Program of the Institute of Government at the University of North Carolina at Chapel Hill, the North Carolina Effective Principal Training Program of the Personnel Services Area of the North Carolina State Department of Public Instruction, the North Carolina Assessment Center of the National Association of Secondary School Principals and the Leadership Institute of the Personnel Services Area of the North Carolina Department of Public Instruction, and the Initial Certification Program for Administrators and Curriculum-Instructional Specialists of the Division of Teacher Education of the North Carolina Department of Public Instruction

Functional characterization and signalling systems of corazonin and red pigment concentrating hormone in the green shore crab, Carcinus maenas

Neuropeptides play a central role as neurotransmitters, neuromodulators and hormones in orchestrating arthropod physiology. The post-genomic surge in identified neuropeptides and their putative receptors has not been matched by functional characterization of ligand-receptor pairs. Indeed, until very recently no G protein-coupled receptors (GPCRs) had been functionally defined in any crustacean. Here we explore the structurally-related, functionally-diverse gonadotropin-releasing hormone paralogs, corazonin (CRZ) and red-pigment concentrating hormone (RPCH) and their G-protein coupled receptors (GPCRs) in the crab, Carcinus maenas. Using aequorin luminescence to measure in vitro Ca2+ mobilization we demonstrated receptor-ligand pairings of CRZ and RPCH. CRZR-activated cell signaling in a dose-dependent manner (EC50 0.75 nM) and comparative studies with insect CRZ peptides suggest that the C-terminus of this peptide is important in receptor-ligand interaction. RPCH interacted with RPCHR with extremely high sensitivity (EC50 20 pM). Neither receptor bound GnRH, nor the AKH/CRZ-related peptide. Transcript distributions of both receptors indicate that CRZR expression was, unexpectedly, restricted to the Y-organs (YO). Application of CRZ peptide to YO had no effect on ecdysteroid biosynthesis, excepting a modest stimulation in early post-molt. CRZ had no effect on heart activity, blood glucose levels, lipid mobilization or pigment distribution in chromatophores, a scenario that reflected the distribution of its mRNA. Apart from the well-known activity of RPCH as a chromatophorotropin, it also indirectly elicited hyperglycemia (which was eyestalk-dependent). RPCHR mRNA was also expressed in the ovary, indicating possible roles in reproduction. The anatomy of CRZ and RPCH neurons in the nervous system is described in detail by immunohistochemistry and in situ hybridization. Each peptide has extensive but non-overlapping distribution in the CNS, and neuroanatomy suggests that both are possibly released from the post-commissural organs. This study is one of the first to deorphanize a GPCR in a crustacean and to provide evidence for hitherto unknown and diverse functions of these evolutionarily-related neuropeptides

Improved single-chain transactivators of the Tet-On gene expression system

BACKGROUND: The Tet-Off (tTA) and Tet-On (rtTA) regulatory systems are widely applied to control gene expression in eukaryotes. Both systems are based on the Tet repressor (TetR) from transposon Tn10, a dimeric DNA-binding protein that binds to specific operator sequences (tetO). To allow the independent regulation of multiple genes, novel Tet systems are being developed that respond to different effectors and bind to different tetO sites. To prevent heterodimerization when multiple Tet systems are expressed in the same cell, single-chain variants of the transactivators have been constructed. Unfortunately, the activity of the single-chain rtTA (sc-rtTA) is reduced when compared with the regular rtTA, which might limit its application. RESULTS: We recently identified amino acid substitutions in rtTA that greatly improved the transcriptional activity and doxycycline-sensitivity of the protein. To test whether we can similarly improve other TetR-based gene regulation systems, we introduced these mutations into tTA and sc-rtTA. Whereas none of the tested mutations improved tTA activity, they did significantly enhance sc-rtTA activity. We thus generated a novel sc-rtTA variant that is almost as active and dox-sensitive as the regular dimeric rtTA. This variant was also less sensitive to interference by co-expressed TetR-based tTS repressor protein and may therefore be more suitable for applications where multiple TetR-based regulatory systems are used. CONCLUSION: We developed an improved sc-rtTA variant that may replace regular rtTA in applications where multiple TetR-based regulatory systems are used

Physical Conditions in the Dwarf Local Group Irregular Galaxy IC10. I- Diffuse Ionized Gas

A detailed analysis on the physical conditions of the Interstellar Medium in the main body of the Local Group dwarf irregular galaxy IC 10 is carried out using long-slit spectroscopy. Maps of the excitation, the H$\alpha$ flux, [NII]/H$\alpha$ and [SII]/H$\alpha$ are presented. The Diffuse Ionized Gas inside the galaxy is studied. We found that the spectral characteristics are not similar to those for spiral galaxies: the values of the ratios [NII]/H$\alpha$ and [SII]/H$\alpha$ are not as large as in spiral galaxies but the excitation is much larger. These values, especially the large excitation, can be explained by proposing an extra source of ionization. Different sources are studied but any of the models at hand could be really fitted except a combination of photons leaking from the HII regions in addition to the ionization provided by the WR stars present in this galaxy. Shocks are not needed for explaining the line intensity ratios except in a small part in the west side of the galaxy.Comment: 12 pages, 12 figures, accepted for publication in A&

Use of Avian Bornavirus Isolates to Induce Proventricular Dilatation Disease in Conures

The fulfillment of Koch’s postulates shows that the virus causes proventricular dilatation disease in parrots