An implant periapical lesion associated with presence of residual root fragments: Report of case treated with apicoectomy

Indexación: Scopus.Chemicals and CAS Registry Numbers: azithromycin, 83905-01-5, 117772-70-0, 121470-24-4; chlorhexidine, 3697-42-5, 55-56-1; erythromycin, 114-07-8, 70536-18-4The present study reports a case of a periapical lesion in an implant placed in an alveolar ridge which presented a residual root fragment of a deciduous tooth. Patient attended a dental clinic because she wanted to replace the missing tooth with an implant. She reported having lost the temporary tooth for one year. The initial Cone-beam Computerised Tomography image showed an edentulous area in the #13 zone, and the presence of a fragment of the deciduous tooth. The small root fragment was removed and an implant 4.0 × 13 mm amplified was installed. Patient presented acute pain and inflammation at the implant site 20 d after the surgery when the implant was originally installed; the first periapical surgery was carried out. Four months after the first periapical surgery, in periapical x-ray a radiolucent lesion was observed surrounding the apical region of the implant with a dry sound under percussion. The second periapical surgery was carried out. Five months after the second periapical surgery, the presence of a fistula was observed clinically, while in X-ray a radiolucent lesion was observed in the apex of the implant. Implant-plasty was carried out, removing approximately 5 mm of the apical portion of the implant. This treatment was successful after 27 months' monitoring. © 2018, Scientific Publishers of India. All rights reserved.https://www.alliedacademies.org/articles/an-implant-periapical-lesion-associated-with-presence-of-residual-root-fragments-report-of-case-treated-with-apicoectomy-10489.htm

Single standard calibration and data processing in flow injection titration based on concentration gradients

This paper describes use of gradients of concentration generated in flow injection (FI) systems to perform determinations based on points where the concentration of titrant and analyte are at stoichiometric ratio. Two procedures were developed. In one procedure the titrant is injected in a FI manifold and merges with the sample which is continuously pumped towards the detector. In the other procedure the sample is injected and merged with the titrant which is continuously pumped. Both techniques make use of concentration gradients of the sample or titrant generated in FI manifolds that contain a mixing chamber. This gradient is calibrated employing only one standard solution (usually the titrant) in order to convert any detector signal, obtained in the elapsed time after injection, to instantaneous concentration values. The flow system is microcomputer controlled and data are treated to locate points where the concentration of titrant and analyte are at the stoichiometric ratio. These points are found in abrupt changes of the signal × concentration curves obtained in the presence of the reaction. The method has been evaluated for determination of Fe(II) and acetic acid by spectrophotometric and conductimetric detection, respectively. Results show a mean relative standard deviation lower than 1%, an average accuracy of 1% and a high sampling processing capability (40 to 60 samples per hour)

THE HOLY SEE FOREIGN POLICY TOWARDS THE POLISH POLITICAL TRANSITION: THE ROLE OF POPE JOHN PAUL II (1978-1989)

This paper uses the method of process tracing in order to follow how the change in the Vatican leadership on the year of 1978 contributed to the shift in its foreign policy towards an anti-communism policy that helped on the fall of the Polish communist regime. In the late 1970s, the Vatican influenced the political change on some Communist countries. The final years of the Cold War introduced a range of new actors that contributed to the American preponderance in the international system after the demise of the USSR. Among them, we can highlight the rebirth of the oldest diplomacy in the world, which was able to be among the high players in the system once again: the Holy See. Also, the cooperation between the American president Ronald Reagan and Pope John Paul II is a controversial point that must be investigated. It is possible to assert, however, that Karol Wojtyła played an essential role during the Polish political transition and that it was possible because of the partnership that His Holiness developed with Ronald Reagan

An overview of data‐driven HADDOCK strategies in CAPRI rounds 38-45

Our information-driven docking approach HADDOCK has demonstrated a sustained performance since the start of its participation to CAPRI. This is due, in part, to its ability to integrate data into the modeling process, and to the robustness of its scoring function. We participated in CAPRI both as server and manual predictors. In CAPRI rounds 38-45, we have used various strategies depending on the available information. These ranged from imposing restraints to a few residues identified from literature as being important for the interaction, to binding pockets identified from homologous complexes or template-based refinement/CA-CA restraint-guided docking from identified templates. When relevant, symmetry restraints were used to limit the conformational sampling. We also tested for a large decamer target a new implementation of the MARTINI coarse-grained force field in HADDOCK. Overall, we obtained acceptable or better predictions for 13 and 11 server and manual submissions, respectively, out of the 22 interfaces. Our server performance (acceptable or higher-quality models when considering the top 10) was better (59%) than the manual (50%) one, in which we typically experiment with various combinations of protocols and data sources. Again, our simple scoring function based on a linear combination of intermolecular van der Waals and electrostatic energies and an empirical desolvation term demonstrated a good performance in the scoring experiment with a 63% success rate across all 22 interfaces. An analysis of model quality indicates that, while we are consistently performing well in generating acceptable models, there is room for improvement for generating/identifying higher quality models

It Takes Two to Tango, Part II : Synthesis of A-Ring Functionalised Quinones Containing Two Redox-Active Centres with Antitumour Activities

In 2021, our research group published the prominent anticancer activity achieved through the successful combination of two redox centres (ortho-quinone/para-quinone or quinone/seleniumcontaining triazole) through a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. The combination of two naphthoquinoidal substrates towards a synergetic product was indicated, but not fully explored. Herein, we report the synthesis of 15 new quinone-based derivatives prepared from click chemistry reactions and their subsequent evaluation against nine cancer cell lines and the murine fibroblast line L929. Our strategy was based on the modification of the A-ring of paranaphthoquinones and subsequent conjugation with different ortho-quinoidal moieties. As anticipated, our study identified several compounds with IC50 values below 0.5 µM in tumour cell lines. Some of the compounds described here also exhibited an excellent selectivity index and low cytotoxicity on L929, the control cell line. The antitumour evaluation of the compounds separately and in their conjugated form proved that the activity is strongly enhanced in the derivatives containing two redox centres. Thus, our study confirms the efficiency of using A-ring functionalized para-quinones coupled with ortho-quinones to obtain a diverse range of two redox centre compounds with potential applications against cancer cell lines. Here as well, it literally takes two for an efficient tango

Annotation of rule-based models with formal semantics to enable creation, analysis, reuse and visualization

Motivation: Biological systems are complex and challenging to model and therefore model reuse is highly desirable. To promote model reuse, models should include both information about the specifics of simulations and the underlying biology in the form of metadata. The availability of computationally tractable metadata is especially important for the effective automated interpretation and processing of models. Metadata are typically represented as machine-readable annotations which enhance programmatic access to information about models. Rule-based languages have emerged as a modelling framework to represent the complexity of biological systems. Annotation approaches have been widely used for reaction-based formalisms such as SBML. However, rule-based languages still lack a rich annotation framework to add semantic information, such as machine-readable descriptions, to the components of a model. Results: We present an annotation framework and guidelines for annotating rule-based models, encoded in the commonly used Kappa and BioNetGen languages. We adapt widely adopted annotation approaches to rule-based models. We initially propose a syntax to store machine-readable annotations and describe a mapping between rule-based modelling entities, such as agents and rules, and their annotations. We then describe an ontology to both annotate these models and capture the information contained therein, and demonstrate annotating these models using examples. Finally, we present a proof of concept tool for extracting annotations from a model that can be queried and analyzed in a uniform way. The uniform representation of the annotations can be used to facilitate the creation, analysis, reuse and visualization of rule-based models. Although examples are given, using specific implementations the proposed techniques can be applied to rule-based models in general