The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth

The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood. Here, we show that in Arabidopsis thaliana, the extracellular peptide RALF1 and its receptor, the FERONIA receptor kinase, promote root hair (RH) tip growth by modulating protein synthesis. We found that RALF1 promotes FERONIA-mediated phosphorylation of eIF4E1, a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate. Phosphorylated eIF4E1 increases mRNA affinity and modulates mRNA translation and, thus, protein synthesis. The mRNAs targeted by the RALF1–FERONIA–eIF4E1 module include ROP2 and RSL4, which are important regulators of RH cell polarity and growth. RALF1 and FERONIA are expressed in a polar manner in RHs, which facilitate eIF4E1 polar localization and thus may control local ROP2 translation. Moreover, we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter, determining the final RH size. Our study reveals that the link between RALF1–FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.Fil: Zhu, Sirui. Hunan University; ChinaFil: Estevez, Jose Manuel. Universidad Andrés Bello; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Liao, Hongdong. Hunan University; ChinaFil: Zhu, Yonghua. Hunan University; ChinaFil: Yang, Tao. Central South University of Forestry and Technology; ChinaFil: Li, Chiyu. Hunan University; ChinaFil: Wang, Yichuan. Southern University of Science and Technology; ChinaFil: Li, Lan. Hunan University; ChinaFil: Liu, Xuanming. Hunan University; ChinaFil: Martinez Pacheco, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Guo, Hongwei. Southern University of Science and Technology; ChinaFil: Yu, Feng. Hunan University; Chin

RALF1-FERONIA complex affects splicing dynamics to modulate stress responses and growth in plants

Wang L, Yang T, Wang B, et al. RALF1-FERONIA complex affects splicing dynamics to modulate stress responses and growth in plants. SCIENCE ADVANCES. 2020;6(21): eaaz1622.The environmentally responsive signaling pathways that link global transcriptomic changes through alternative splicing (AS) to plant fitness remain unclear. Here, we found that the interaction of the extracellular rapid alkalinization FACTOR 1 (RALF1) peptide with its receptor FERONIA (FER) triggered a rapid and massive RNA AS response by interacting with and phosphorylating glycine-rich RNA binding protein7 (GRP7) to elevate GRP7 nuclear accumulation in Arabidopsis thaliana. FER-dependent GRP7 phosphorylation enhanced its mRNA binding ability and its association with the spliceosome component U1-70K to enable splice site selection, modulating dynamic AS. Genetic reversal of a RALF1-FER-dependent splicing target partly rescued mutants deficient in GRP7. AS of GRP7 itself induced nonsense-mediated decay feedback to the RALF1-FER-GRP7 module, fine-tuning stress responses, and cell growth. The RALF1-FER-GRP7 module provides a paradigm for regulatory mechanisms of RNA splicing in response to external stimuli

Prevalence and Related Factors of Insomnia Among Chinese Medical Staff in the Middle and Late Stage of COVID-19

Background: The outbreak of novel coronavirus disease (COVID-19) has brought serious psychological pressure to people, especially medical health staff. At present, there are few studies on insomnia and related factors of medical health staff in the middle and late stage of the epidemic of COVID-19. Therefore, the purpose of this study was to investigate the prevalence of insomnia and its related risk factors among medical workers in China in the middle and later stage of COVID-19 epidemic, as well as the relationship between insomnia and psychological resilience. Methods: From February 14 to March 29, 2020, a cross-sectional survey was conducted among 606 medical staff in China through Ranxing Technology&#39;s &quot;SurveyStar&quot; network platform. All subjects were assessed with the Insomnia Severity Index (ISI) and simplified Chinese version of Connor-Davidson Resilience scale (CD-RISC-10). Results: In the middle and later stages of the COVID-19 outbreak, the incidence of insomnia among medical staff was 32.0%. Compared with non-insomnia group, the insomnia group had younger age, lower education level, longer daily working hours and less psychological resilience. In addition, the prevalence of insomnia was higher in medical staff with a history of somatic diseases. The severity of insomnia of Chinese medical staff was associated with age, education level, daily working hours, psychological resilience and somatic diseases. Conclusions: Our study shows that nearly 1/3 of Chinese medical workers suffer from insomnia nearly a month after the COVID-19 outbreak. Compared with the general population, medical staff who are working with COVID are more prone to insomnia. Risk factors for insomnia include younger age, lower education level, longer working hours per day, and physical illness. The tenacious dimension of psychological resilience is a protective factor for insomnia.</p

Evaluation of health problems and the impact of compensation activities for the professional golf players

Title: Evaluation of health problems and the impact of compensation activities for the professional golf players Objectives: The main aim of this thesis is to describe the most common health problems in golf and to further evaluate the questionnaire form of the most common health problems of the golf players in the major league and the first league of golf in the Czech Republic in connection to a frequent training, playing tournament rounds, and their compensation. Methods: The method of research and the method of written questioning is used in this thesis. Theoretical information was obtained mainly from the foreign sources and information for the evaluation was obtained by non-standardized questionnaires of the first league golf players and of the major golf league competition in the Czech Republic. Total of 115 players were approached, the data were processed by 75 players (65% return). Microsoft Excel software was used for the data analysis. Results: During the main golf season in 2015, 37 golf players had injury that prevented them from playing and training golf, this corresponds to 49% of the golf players. Evaluated data show, that the most common problem among the golf players, is the vulnerability and pain in the wrist tendons, 11 players were struggling with these problems. Another health..

Outsourcing Agricultural Production: Evidence from Rice Farmers in Zhejiang Province

China has recorded positive growth rates of grain production for the past eleven consecutive years. This is a remarkable accomplishment given that China's rapid industrialization and urbanization has led to a vast reduction of arable land and agricultural labor to non-agricultural sectors. While there are many factors contributing to this happy outcome, one potential contributing factor that has received increasing attention is the emergence of agricultural production outsourcing, a new rural institution that has emerged in recent years. This study aims to contribute to the limited but growing literature on agricultural production outsourcing in China. Specifically, this study analyzes factors affecting farmers' decisions to outsource any or some production tasks using data from rice farmers in Zhejiang province. Results from a logistic model show that farm size and government subsidy encourages farmers to outsource while ownership of agricultural machines and land fragmentation have negative effects on farmers' decisions to outsource production tasks. Results also showed that determinants of outsourcing decisions vary with the production tasks that farmers outsourced