Letter to the Editor: a response to Horne and Lucey (2017)

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Ionospheric longitudinal variations at midlatitudes: Incoherent scatter radar observation at Millstone Hill

Incoherent scatter radar (ISR) extra-wide coverage experiments during the period of 1978–2011 at Millstone Hill are used to investigate longitudinal differences in electron density. This work is motivated by a recent finding of the US east-west coast difference in TEC suggesting a combined effect of changing geomagnetic declination and zonal winds. The current study provides strong supporting evidence of the longitudinal change and the plausible mechanism by examining the climatology of electron density Ne on both east and west sides of the radar with a longitude separation of up to 40o for different heights within 300–450 km. Main findings include: 1) The east-west difference can be up to 60% and varies over the course of the day, being positive (East side Ne > West side Ne) in the late evening, and negative (West side Ne > East side Ne) in the pre-noon. 2) The east-west difference exists throughout the year. The positive (relative) difference is most pronounced in winter; the negative (relative) difference is most pronounced in early spring and later summer. 3) The east-west difference tends to enhance toward decreasing solar activity, however, with some seasonal dependence; the enhancements in the positive and negative differences do not take place simultaneously. 4) Both times of largest positive and largest negative east-west differences in Ne are earlier in summer and later in winter. The two times differ by 12–13 h, which remains constant throughout the year. 5) Variations at different heights from 300–450 km are similar. Zonal wind climatology above Millstone Hill is found to be perfectly consistent with what is expected based on the electron density difference between the east and west sides of the site. The magnetic declination-zonal wind mechanism is true for other longitude sectors as well, and may be used to understand longitudinal variations elsewhere. It may also be used to derive thermospheric zonal winds.National Natural Science Foundation (China) (Grant 40890164)National Science Foundation (U.S.) (Grants ATM-0733510 and ATM- 6920184

An On Body Accelerometer System for streaming therapy data using COTS UHF RFID

This paper details the use of an on body Accelerometer COTS RFID system for use in determining specific therapeutic movements, and the performance of said movements. Conventional methodologies rely on power intensive Bluetooth or Wi-Fi technologies for live communications, but this paper outlines the use of ultra-low power battery assistive passive modes on UHF RFID chips to harness sub-2mW continuous streaming of Accelerometer data

Proteostasis and the Regulation of Intra- and Extracellular Protein Aggregation by ATP-Independent Molecular Chaperones: Lens α-Crystallins and Milk Caseins

Conspectus: Molecular chaperone proteins perform a diversity of roles inside and outside the cell. One of the most important is the stabilization of misfolding proteins to prevent their aggregation, a process that is potentially detrimental to cell viability. Diseases such as Alzheimer\u27s, Parkinson\u27s, and cataract are characterized by the accumulation of protein aggregates. In vivo, many proteins are metastable and therefore under mild destabilizing conditions have an inherent tendency to misfold, aggregate, and hence lose functionality. As a result, protein levels are tightly regulated inside and outside the cell. Protein homeostasis, or proteostasis, describes the network of biological pathways that ensures the proteome remains folded and functional. Proteostasis is a major factor in maintaining cell, tissue, and organismal viability. We have extensively investigated the structure and function of intra- and extracellular molecular chaperones that operate in an ATP-independent manner to stabilize proteins and prevent their misfolding and subsequent aggregation into amorphous particles or highly ordered amyloid fibrils. These types of chaperones are therefore crucial in maintaining proteostasis under normal and stress (e.g., elevated temperature) conditions. Despite their lack of sequence similarity, they exhibit many common features, i.e., extensive structural disorder, dynamism, malleability, heterogeneity, oligomerization, and similar mechanisms of chaperone action. In this Account, we concentrate on the chaperone roles of α-crystallins and caseins, the predominant proteins in the eye lens and milk, respectively. Intracellularly, the principal ATP-independent chaperones are the small heat-shock proteins (sHsps). In vivo, sHsps are the first line of defense in preventing intracellular protein aggregation. The lens proteins αA- and αB-crystallin are sHsps. They play a crucial role in maintaining solubility of the crystallins (including themselves) with age and hence in lens proteostasis and, ultimately, lens transparency. As there is little metabolic activity and no protein turnover in the lens, crystallins are very long lived proteins. Lens proteostasis is therefore very different to that in normal, metabolically active cells. Crystallins undergo extensive post-translational modification (PTM), including deamidation, racemization, phosphorylation, and truncation, which can alter their stability. Despite this, the lens remains transparent for tens of years, implying that lens proteostasis is intimately integrated with crystallin PTMs. Many PTMs do not significantly alter crystallin stability, solubility, and functionality, which thereby facilitates lens transparency. In the long term, however, extensive accumulation of crystallin PTMs leads to large-scale crystallin aggregation, lens opacification, and cataract formation. Extracellularly, various ATP-independent molecular chaperones exist that exhibit sHsp-like structural and functional features. For example, caseins, the major milk proteins, exhibit chaperone ability by inhibiting the amorphous and amyloid fibrillar aggregation of a diversity of destabilized proteins. Caseins maintain proteostasis within milk by preventing deleterious casein amyloid fibril formation via incorporation of thousands of individual caseins into an amorphous structure known as the casein micelle. Hundreds of nanoclusters of calcium phosphate are sequestered within each casein micelle through interactions with short, highly phosphorylated casein sequences. This results in a stable biofluid that contains a high concentration of potentially amyloidogenic caseins and concentrations of calcium and phosphate that can be far in excess of the solubility of calcium phosphate. Casein micelle formation therefore performs vital roles in neonatal nutrition and calcium homeostasis in the mammary gland

Purification and partial characterization of draculin, the anticoagulant factor present in the saliva of vampire bats (<i>Desmodus rotundus</i>)

From the saliva of the vampire bat Desmodus rotundus, we isolated an unknown anticoagulant protein which we have named draculin. Its molecular mass as determined by non-reduced SDS-PAGE is about 83 kDa. The reduced polypeptide shows a slower migration. HPLC in a molecular sieve matrix yields a single, symmetrical peak corresponding to 88.5 kDa. Isoelectric focusing shows an acidic protein with pI = 4.1–4.2. Aminoacid analysis is compatible with a single chain polypeptide of about 80 kDa. Cyanogen bromide cleavage yields a single 16-aminoacid peptide, corresponding to the amino-terminus of the native molecule. Draculin inhibits the activated form of coagulation factors IX and X. It does not act on thrombin, trypsin, chymotrypsin and does not express fibrinolytic activity. The inhibition is immediate and not readily reversible, with a stoichiometry of about two molecules of draculin per molecule of factor IXa or Xa. Surprisingly, the inhibitory activity against either factor is not affected by the presence of the other. Draculin binds quantitatively to either immobilised factor Xa or factor IXa. Our preliminary interpretation is that there are two forms of draculin that hardly differ in structure. Both bind to factor Xa and to factor IXa but one form inhibits factor Xa and the other inhibits factor IXa. When added to plasma, draculin increases the lag phase as well as the height of the peak of thrombin generation

Thermospheric poleward wind surge at midlatitudes during great storm intervals

We report a significant poleward surge in thermospheric winds at subauroral and midlatitudes following the 17–18 March 2015 great geomagnetic storm. This premidnight surge is preceded by strong westward winds. These disturbances were observed over three sites with geodetic latitudes 35–42°N in the American sector by Fabry-Perot interferometers at 630 nm wavelength. Prior to the wind disturbances, subauroral polarization streams (SAPS) were measured by the Millstone Hill incoherent scatter radar between 20 and 02 UT. We identify the observed neutral wind variations as driven by SAPS, through a scenario where strong ion flows cause a westward neutral wind, subsequently establishing a poleward wind surge due to the poleward Coriolis force on that westward wind. These regional disturbances appear to have prevented the well-known storm time equatorward wind surge from propagating into low latitudes, with the consequence that the classic disturbance dynamo mechanism failed to occur.United States. National Aeronautics and Space Administration (Living with a Star NNX15AB83G

The effectiveness and cost-effectiveness of telephone triage of patients requesting same day consultations in general practice: study protocol for a cluster randomised controlled trial comparing nurse-led and GP-led management systems (ESTEEM)

notes: PMCID: PMC3574027This is a freely-available open access publication. Please cite the published version which is available via the DOI link in this record.Recent years have seen an increase in primary care workload, especially following the introduction of a new General Medical Services contract in 2004. Telephone triage and telephone consultation with patients seeking health care represent initiatives aimed at improving access to care. Some evidence suggests that such approaches may be feasible but conclusions regarding GP workload, cost, and patients' experience of care, safety, and health status are equivocal. The ESTEEM trial aims to assess the clinical- and cost-effectiveness of nurse-led computer-supported telephone triage and GP-led telephone triage, compared to usual care, for patients requesting same-day consultations in general practice.UK National Institute of Health Research Health Technology Assessment programmeDepartment of Healt

Families’ perceptions of and experiences related to a pediatric weight management program.

Objective: To examine parents' and children's perceptions of and experiences related to a Parents as Agents of Change (PAC) intervention for managing pediatric obesity. Methods: Ten families were recruited from a PAC intervention. Participants were interviewed before (10 adults and 9 children), during (9 adults and 8 children), and after (8 adults) the intervention. Results: Before the intervention, families reported goals to increase physical activity, plan and eat healthier meals, reduce screen time, and lose weight. During the intervention, families described different approaches to making behavior changes depending on who assumed responsibility (parent, child, or shared responsibility). After the intervention, group setting, goal setting, and portion size activities were viewed positively. Suggestions for improvement included engaging children and reducing intervention length. Conclusions and Implications: Practitioners delivering PAC interventions should discuss families' goals and concerns, and who is responsible for making lifestyle changes. Practical activities are valuable. The length of interventions and engagement of children should be considere