3 research outputs found
Aeromonas hydrophila subsp. dhakensis – a causative agent of gastroenteritis imported into the Czech Republic
Out of the twenty-one A. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two
A. hydrophila subsp. dhakensis isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct
taxonomic position of the A. hydrophila subsp. dhakensis CCM 7329 was verified by cpn60 sequencing (GeneBank accession
number HM536193). The remaining A. hydrophila complex isolates were identified as A. hydrophila subsp. hydrophila. The
ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both A. hydrophila subsp. dhakensis and
A. hydrophila subsp. hydrophila was limited. In contrast to the A. hydrophila subsp. hydrophila, the faecal isolates of A. hydrophila
subsp. dhakensis did not produce acid from arbutin. When compared in a two-dimensional plot, the A. hydrophila subsp.
dhakensis faecal isolates contained higher amounts of the two minor fatty acids C13:0 and C17:1 ω8c than the A. hydrophila
subsp. hydrophila reference strain. This is the first detected occurrence of the less frequent A. hydrophila subsp. dhakensis
in our region and ribotyping was proved as a suitable method for the identification of A. hydrophila subsp. dhakensis