Strategies for Accelerating the Development of Catalytic Enantioselective Reactions

The development of enantioselective catalytic processes for the manufacture of chiral intermediates is a very complex endeavor and can be very time consuming and expensive. In this contribution the major issues which might lead to long development times will be discussed and strategies to deal with these problems are described. The general part is illustrated with the approach Solvias has chosen for assisting and supporting the development of enantioselective homogeneous hydrogenation processes, at the moment the most important industrial application of asymmetric catalysis. Special emphasis is given to the application of high-throughput screening (HTS) using a Symyx HiP system and the description of the Solvias portfolio of chiral ligands which makes a broad variety of diphosphine ligands available for all phases of process development from the first screening experiments to the large-scale manufacturing phase. Four case histories serve to illustrate the generic description of the development process

Ocean Floor Observation and Bathymetry System (OFOBS): A new Towed Camera/Sonar System for Deep-Sea Habitat Surveys

Towed camera systems are commonly used to collect photo and video images of the deep seafloor for a wide variety of purposes, from pure exploratory research to the development of management plans. Ongoing technological developments are increasing the quantity and quality of data collected from the deep seafloor. Despite these improvements, the area of seafloor, which towed systems can survey, optically remains limited by the rapid attenuation of visible wavelengths within water. We present an overview of a new towed camera platform integrating additional acoustical devices: the ocean floor observation and bathymetry system (OFOBS). The towed system maintains continuous direct communication via fiber optic cable with a support vessel, operational at depths up to 6000 m. In addition to collecting seafloor photo and video data, OFOBS gathers sidescan data over a 100-m swath width. OFOBS functionality is further augmented by a forward looking sonar, used to aid in hazard avoidance and real-time course correction. Data collected during the first field deployments of OFOBS, at a range of seamounts on the Langseth Ridge/Gakkel Ridge intersection (86° N, 61° E) in the high Arctic in September 2016, are presented to demonstrate the functionality of the system. Collected from a location with near continuous ice cover, this explanatory data set highlights the advantages of the system for deep-sea survey work in environments currently difficult to access for the majority of subsurface research platforms

PlasPI marine cameras: Open-source, affordable camera systems for time series marine studies

Imaging underwater can be particularly problematic and expensive given the harsh environmental conditions posed by salinity and for some deployments, pressure. To counter these difficulties, expensive waterproof pressure resistant housings are often used, commonly built from expensive materials such as titanium, if intended for long duration deployments. Further, environmental investigations often benefit from replicate data collection, which additionally increases study costs. In this paper we present a new camera system, developed with off the shelf and 3D printed cost effective components for use in shallow waters of <150 m depth. Integrating Raspberry Pi Zero W microcomputers with open source design files and software, it is hoped these camera systems will be of interest to the global marine and freshwater research communities

Arctic Seafloor Integrity Cruise No. MSM95 – (GPF 19-2_05)

The main aim of the MSM95 research expedition was to investigate and map physical impacts on the arctic seafloor in two distinct and contrasting Arctic areas (The Svalbard shelf edge and the HAUSGARTEN time series stations in the FRAM strait) with a range of research equipment. A ‘nested’ data approach was conducted in each research area, with broad seafloor mapping conducted initially with the R/V MARIA S. MERIAN onboard acoustic systems (The EM122 and EM712 bathymetric systems), followed by focused subsequent mapping conducted by PAUL 3000 automated underwater vehicle (AUV) sidescan and camera deployments, Ocean Floor Observation and Bathymetry System (OFOBS) towed sidescan and camera trawls and finally with very high resolution investigations conducted with a new mini-ROV launched directly from the OFOBS for close seafloor visual analysis. These data will be used to produce spatial distribution maps of iceberg and fishery impacts on the seafloor at three locations to the north, south and west of the Svalbard Archipelago, as well as maps of drop stone and topography variations across several of the HAUSGARTEN stations

Depression chains in seafloor of contrasting morphology, Atacama Trench margin: a comment on Marsh et al. (2018)

This comment presents acoustic and visual data showing deep seafloor depression chains similar to those reported in Marsh et al. (R. Soc. open sci. 5: 180286), though from a different deep-sea setting. Marsh et al. present data collected during cruise JC120 from polymetallic nodule rich sites within the Clarion-Clipperton Fracture Zone (CCFZ), at water depths of between 3999 and 4258 m. Within this comment, we present data collected with equivalent acoustic and imaging devices on-board the RV Sonne (SO261—March/April 2018) from the Atacama Trench, approximately 4000 m depth, which shows comparable depression chains in the seafloor. In contrast with the CCFZ observations, our study area was wholly free of polymetallic nodules, an observation therefore weakening the ‘ballast collection’ by deep-sea diving mammals formation hypothesis discussed in their paper. We support their alternate hypothesis that if these features are indeed generated by deep-diving megafauna, then they are more likely the resultant traces of infauna feeding or marks made during opportunistic capture of benthic fish/cephalopods. We observed these potential prey fauna with lander and towed camera systems during the cruise, with example images of these presented here. Both the SO261 and JC120 cruises employed high-resolution sidescan systems at deployment altitudes seldom used routinely until the last few years during scientific deep-sea surveys. Given that both cruises found these depression chains in contrasting physical regions of the East Pacific, they may have a more ubiquitous distribution than at just these sites. Thus, the impacts of cetacean foraging behaviour on deep seafloor communities, and the potential relevance of these prey sources to deep-diving species, should be considered

RV Kronprins Håkon (cruise no. 2019708) Longyearbyen – Longyearbyen 19.09. – 16.10.2019

The HACON cruise is a major component of the FRINATEK HACON project, which aims at investigating the role of the Gakkel Ridge and Arctic Ocean in biological connectivity amongst ocean basins and global biogeography of chemosynthetic ecosystems. The HACON study area is centered in the Aurora seamount and Aurora vent field

LTER HAUSGARTEN 2018 - Long-Term Ecological Research in the Fram Strait, Cruise No. MSM77, September 15 - October 13, 2018, Longyearbyen (Svalbard) - Edinburgh (Scotland)

The 77th cruise of the RV MARIA S. MERIAN contributed to various large national and international research and infrastructure projects (FRAM, ARCHES, INTAROS, ICOS, SIOS) as well as to the research programme PACES-II (Polar Regions and Coasts in the changing Earth System) of the Alfred-Wegener-Institute Helmholtz-Center for Polar and Marine Research (AWI). Investigations within Work Package 4 (Arctic sea ice and its interaction with ocean and ecosystems) of the PACES-II programme, aim at assessing and quantifying ecosystem changes from surface waters to the deep ocean in response to the retreating sea ice, and at exploring the most important (feedback) processes determining temporal and spatial variability. Contributions to the PACES-II Work Package 6 (Large scale variability and change in polar benthic biota and ecosystem functions) include the identification of spatial patterns and temporal trends in relevant benthic community functions, and the development of a comprehensive science community reference collection of observational data. Work carried out within WPs 4 and 6 will support the time-series studies at the LTER (Long-Term Ecological Research) observatory HAUSGARTEN (Fig. 1.1), where we document Global Change induced environmental variations on a polar deep-water ecosystem. This work is carried out in close co-operation between the HGF-MPG Joint Research Group on Deep-Sea Ecology and Technology and the PEBCAO Group (Phytoplankton Ecology and Biogeochemistry in the Changing Arctic Ocean) at AWI as well as the working group Microbial Geochemistry at the GEOMAR and the HGF Young Investigators Group SEAPUMP (Seasonal and regional food web interactions with the biological pump)

Acute childhood diarrhoea in northern Ghana: epidemiological, clinical and microbiological characteristics

<p>Abstract</p> <p>Background</p> <p>Acute diarrhoea is a major cause of childhood morbidity and mortality in sub-Saharan Africa. Its microbiological causes and clinico-epidemiological aspects were examined during the dry season 2005/6 in Tamale, urban northern Ghana.</p> <p>Methods</p> <p>Stool specimens of 243 children with acute diarrhoea and of 124 control children were collected. Patients were clinically examined, and malaria and anaemia were assessed. Rota-, astro-, noro- and adenoviruses were identified by (RT-) PCR assays. Intestinal parasites were diagnosed by microscopy, stool antigen assays and PCR, and bacteria by culturing methods.</p> <p>Results</p> <p>Watery stools, fever, weakness, and sunken eyes were the most common symptoms in patients (mean age, 10 months). Malaria occurred in 15% and anaemia in 91%; underweight (22%) and wasting (19%) were frequent. Intestinal micro-organisms were isolated from 77% of patients and 53% of controls (<it>P </it>< 0.0001). The most common pathogens in patients were rotavirus (55%), adenovirus (28%) and norovirus (10%); intestinal parasites (5%) and bacteria (5%) were rare. Rotavirus was the only pathogen found significantly more frequently in patients than in controls (odds ratio 7.7; 95%CI, 4.2–14.2), and was associated with young age, fever and watery stools. Patients without an identified cause of diarrhoea more frequently had symptomatic malaria (25%) than those with diagnosed intestinal pathogens (12%, <it>P </it>= 0.02).</p> <p>Conclusion</p> <p>Rotavirus-infection is the predominant cause of acute childhood diarrhoea in urban northern Ghana. The abundance of putative enteropathogens among controls may indicate prolonged excretion or limited pathogenicity. In this population with a high burden of diarrhoeal and other diseases, sanitation, health education, and rotavirus-vaccination can be expected to have substantial impact on childhood morbidity.</p

De novo Assembly of a 40 Mb Eukaryotic Genome from Short Sequence Reads: Sordaria macrospora, a Model Organism for Fungal Morphogenesis

Filamentous fungi are of great importance in ecology, agriculture, medicine, and biotechnology. Thus, it is not surprising that genomes for more than 100 filamentous fungi have been sequenced, most of them by Sanger sequencing. While next-generation sequencing techniques have revolutionized genome resequencing, e.g. for strain comparisons, genetic mapping, or transcriptome and ChIP analyses, de novo assembly of eukaryotic genomes still presents significant hurdles, because of their large size and stretches of repetitive sequences. Filamentous fungi contain few repetitive regions in their 30–90 Mb genomes and thus are suitable candidates to test de novo genome assembly from short sequence reads. Here, we present a high-quality draft sequence of the Sordaria macrospora genome that was obtained by a combination of Illumina/Solexa and Roche/454 sequencing. Paired-end Solexa sequencing of genomic DNA to 85-fold coverage and an additional 10-fold coverage by single-end 454 sequencing resulted in ∼4 Gb of DNA sequence. Reads were assembled to a 40 Mb draft version (N50 of 117 kb) with the Velvet assembler. Comparative analysis with Neurospora genomes increased the N50 to 498 kb. The S. macrospora genome contains even fewer repeat regions than its closest sequenced relative, Neurospora crassa. Comparison with genomes of other fungi showed that S. macrospora, a model organism for morphogenesis and meiosis, harbors duplications of several genes involved in self/nonself-recognition. Furthermore, S. macrospora contains more polyketide biosynthesis genes than N. crassa. Phylogenetic analyses suggest that some of these genes may have been acquired by horizontal gene transfer from a distantly related ascomycete group. Our study shows that, for typical filamentous fungi, de novo assembly of genomes from short sequence reads alone is feasible, that a mixture of Solexa and 454 sequencing substantially improves the assembly, and that the resulting data can be used for comparative studies to address basic questions of fungal biology

Seafloor images collected during the 'HACON expedition' with the RV Kronprins Haakon (cruise no. 2019708) (HK19) to the AURORA seamount, high Arctic, 19/09/2019 - 16/10/2019

The Aurora hydrothermal field sits in the westernmost segment of the Gakkel Ridge within the Western Volcanic Zone which extends for 220 km from 7°W to 3°E. The spreading rate here is 14.5-13.5mm/yr and the ridge axis floor at 4200m depth is bounded by steep normal fault rift valley walls and punctuated by a series of axial volcanic ridges and smaller volcanic mounds that rise up hundreds of meters above that axial floor depth. The Aurora field was first located associated with one such volcanic mound as part of the InterRidge two-icebreaker AMORE expedition in 2001. At 82°53'N, 6°15'W a small volcanic mound measuring ~1.5-2km in extent rises approximately 400m from the seafloor at a saddle-point where the rift- valley narrows from ~20km to ~15km wide. During a return cruise to the site aboard the FS Polarstern in 2014, CTD profiling coupled with water column sampling and CH4, TDMn and He-isotope anomalies revealed clear evidence for ongoing hydrothermal activity including strong evidence from CH4:TDMn ratios of ultramafic influence in the underlying vents. Buoyant plume signals intercepted with the CTD during that cruise suggested at least one source of venting was situated toward the south/southwest of the shallowest summit of the Aurora seamount and OFOS deep-tow camera tows from North to South across that summit revealed deep rifts through the thickly sediment seafloor surrounding the base of the volcanic mound. Those firsts were observed striking approximately E-W (across axis) immediately south of the summit of the mound on at least two OFOS tows. These paired observations (CTD, seafloor imaging) led to first imaging of an active vent at ~3900m depth at a Posidonia position of 82°53.83'N, 006°15.32'W. During 2019, as part of the 'HACON' research program, the icebreaker RV Kronprins Hakon visited the seamount, using date from previous expeditions to conduct 10 Ocean Floor Observation and Bathymetry System (OFOBS) drift stations across the summit and flanks of the seamount. The images collected during these stations are presented here. The Ocean Floor Observation and Bathymetry System (OFOBS) (Purser et al., 2018) is a towed underwater camera sled equipped with both a high resolution photo-camera (iSiTEC, CANON EOS 5D Mark III) and a high-definition video-camera (iSiTEC, Sony FCB-H11). The cameras are mounted on a steel frame (140L x 92W x 135H cm), together with two strobe lights (iSiTEC UW-Blitz 250, TTL driven), three laser pointers at a distance of 50 cm from each other that were used to estimate the size of seafloor structures, four LED lights, and a USBL positioning system (Posidonia) to track the position of the OFOS during deployments. For the duration of cruise no. 2019708 however, the ship Kongsberg transponders were used in place of the Posidonia system for USBL positioning. Positioning information is further augmented via input from an onboard IXBLUE inertial navigation system (INS) with DVL input. The sidescan bathymetry sonar is an interferometric Edgetech 2205 AUV/ROV MPES (Multi Phase Echosounder) with two sidescan frequencies (230 kHz & 540 kHz) for different range and resolution achievements. The transducers additionally hold a bathymetric receive array to calculate bathymetric 2.5D data in the range of the 540 kHz sidescan sonar with around 800 data points per ping. A forward acoustic camera gives ~20m warning of approaching obstacles in front of the OFOBS sled. During deployments, the OFOBS is lowered to ~1.5 m above the seafloor then towed by the ship / ice drift at speeds of up to 0.8 kn. Ideal deployment speed is 0.4 kn, and for the majority of deployments made during this cruise, drift was slower. Every 20 seconds a 26 megapixel still image of the seafloor is taken by the device, and there is the option to additionally take 'hotkey' images of features of interest. The collected images are presented here, with positions based on a splined interpretation of the in-situ Kongsberg transponder position