Distribution and habitat use of a cryptic small cetacean, the Burmeister's porpoise, monitored from a small-scale fishery platform

This is the final version. Available from Frontiers Media via the DOI in this record. There is widespread evidence that small-scale fisheries (SSF) bycatch threatens many populations of small cetaceans, yet conservation efforts are often limited by a lack of basic knowledge regarding their abundance, distribution, and habitat use. Here, we used passive acoustic monitoring from an SSF platform-of-opportunity to better characterize the distribution and habitat use of small cetaceans in northern Peru, focussing on the little-known Burmeister's porpoise Phocoena spinipinnis. From 2009 to 2012, acoustic click detectors (C-PODs) were attached to fishing nets for the duration of 116 fishing sets (30 fishing trips). Dolphins (unspecified delphinids) and porpoises were recorded around 71 and 22% of fishing sets, respectively. The probability of occurrence and buzzing behavior (a proxy for foraging), and time spent, were linked to both static and dynamic environmental variables to examine the drivers of habitat use. Dolphin activity was spread evenly throughout the fishing area and was not linked to any habitat variables. In contrast, porpoises were detected in neritic waters, and habitat models performed well, identifying preferences for shallow (< 200 m depth) and cooler (17-18°C) waters, close (< 50 km) to shore. The high bycatch rate of small cetaceans in Peruvian SSF gave us the unique opportunity to investigate the link between bycatch and cetacean activity around vessels. We found a positive relationship between the likelihood of a bycatch event and acoustic presence for both dolphins and porpoises, however as we did not know the timing of entanglement, we could not link vocalization rates to mortality events. Nonetheless, as small cetaceans (particularly dolphins) frequently encounter fishing boats, the likelihood of entanglements may be reduced through effective efforts to alert animals to the presence of the net, either acoustically (using acoustic alarms) or visually. This study demonstrates that passive acoustic monitoring from a fisheries platform can provide insights into the distribution and habitat use of small cetaceans at relatively low cost, and is likely to be suitable in regions with low monitoring effort and high fishing pressure.Natural Environment Research CouncilEuropean UnionDarwin Initiativ

Where is my sink? Reconstruction of landscape development in 1 southwestern Africa since the Late Jurassic

Quantifying the rates and timing of landscape denudation provides a means to constrain sediment flux through time to offshore sedimentary basins. The Late Mesozoic evolution of drainage basins in southern Africa is poorly constrained despite the presence of several onshore and offshore sedimentary basins. A novel approach has been developed to calculate the volume of material eroded since the Late Jurassic at different time steps by constructing structural cross-sections and extrapolating thicknesses of eroded material. Using different assumptions, the calculated volumes of material eroded from southwestern Africa range from 2.52x10⁶ km³ (11.3 km of vertical thickness removed) to 8.87 x10⁵ km³ (4.0 km of vertical thickness removed). For the southward draining systems alone, the calculated removal of 7.81 x10⁵ – 2.60 x10⁵ km³ of material is far greater than the volumes of sediment recorded in offshore sedimentary basins (268 500 km³). Reconstruction of the drainage systems using geomorphic indicators and clast provenance of the Uitenhage Group, as well as extrapolated surface exposure ages, indicate the southern draining systems were active from the Late Jurassic with coeval activity in axial and transverse drainage systems. The calculated volumes are tied to published apatite fission track (AFT) dates to constrain the changes in exhumation rate through time (using multiple scenarios), which indicate a significant amount of Early Cretaceous exhumation (up to 1.26 x10⁶ km³, equivalent to 5.70km of vertical thickness). For the first time, this has permitted long-term landscape evolution to be used to support the interpretation that some of the ‘missing’ sediment was deposited in sedimentary basins on the Falkland Plateau as it moved past southern Africa during the Early Cretaceous. This implies that in this instance, the sinks are separated from their source areas by ~6000 km

Big catch, little sharks: Insight into Peruvian small-scale longline fisheries.

PublishedJournal ArticleThis is the final version of the article. Available from Wiley Open Access via the DOI in this record.Shark take, driven by vast demand for meat and fins, is increasing. We set out to gain insights into the impact of small-scale longline fisheries in Peru. Onboard observers were used to document catch from 145 longline fishing trips (1668 fishing days) originating from Ilo, southern Peru. Fishing effort is divided into two seasons: targeting dolphinfish (Coryphaena hippurus; December to February) and sharks (March to November). A total of 16,610 sharks were observed caught, with 11,166 identified to species level. Of these, 70.6% were blue sharks (Prionace glauca), 28.4% short-fin mako sharks (Isurus oxyrinchus), and 1% were other species (including thresher (Alopias vulpinus), hammerhead (Sphyrna zygaena), porbeagle (Lamnus nasus), and other Carcharhinidae species (Carcharhinus brachyurus, Carcharhinus falciformis, Galeorhinus galeus). Mean ± SD catch per unit effort of 33.6 ± 10.9 sharks per 1000 hooks was calculated for the shark season and 1.9 ± 3.1 sharks per 1000 hooks were caught in the dolphinfish season. An average of 83.7% of sharks caught (74.7% blue sharks; 93.3% mako sharks) were deemed sexually immature and under the legal minimum landing size, which for species exhibiting k-selected life history traits can result in susceptibility to over exploitation. As these growing fisheries operate along the entire Peruvian coast and may catch millions of sharks per annum, we conclude that their continued expansion, along with ineffective legislative approaches resulting in removal of immature individuals, has the potential to threaten the sustainability of the fishery, its target species, and ecosystem. There is a need for additional monitoring and research to inform novel management strategies for sharks while maintaining fisher livelihoods.We would like to thank the members of the fishing communities who participated in this study, in particular the fishers who were willing to have an observer onboard. We acknowledge Pro Delphinus staff: Bernedo, Cuentas, Lopez, and Mamani, for their help in data collection. We are thankful also to Pro Delphinus staff Natalia Ortiz and Nadia Balducci for support in data entry. This study was conducted in conjunction with and funded by the Darwin Initiative Sustainable Artisanal Fisheries Initiative in Peru and an initial grant from the Oak Foundation through Duke University. MJW was funded by PRIMaRE. JAS and JCM received support from ORSAS and University of Exeter, respectively

Use of a dual reporter plasmid to demonstrate bactofection with an attenuated aroa- derivative of Pasteurella multocida b:2

A reporter plasmid pSRG has been developed which expresses red fluorescent protein (RFP) from a constitutive prokaryotic promoter within Pasteurella multocida B:2 and green fluorescent protein (GFP) from a constitutive eukaryotic promoter within mammalian cells. This construct has been used to determine the location and viability of the bacteria when moving from the extracellular environment into the intracellular compartment of mammalian cells. Invasion assays with embryonic bovine lung (EBL) cells and an attenuated AroA- derivative of Pasteurella multocida B:2 (strain JRMT12), harbouring the plasmid pSRG, showed that RFP-expressing bacteria could be detected intracellularly at 3 h post-invasion. At this stage, some EBL cells harbouring RFP-expressing bacteria were observed to express GFP simultaneously, indicating release of the plasmid into the intracellular environment. At 5 h post-invasion, more EBL cells were expressing GFP, while still harbouring RFP-expressing bacteria. Concurrently, some EBL cells were shown to express only GFP, indicating loss of viable bacteria within these cells. These experiments proved the functionality of the pSRG dual reporter system and the potential of P. multocida B:2 JRMT12 for bactofection and delivery of a DNA vaccine

Bilateral undisplaced insufficiency neck of femur fractures associated with short-term steroid use: a case report

<p>Abstract</p> <p>Introduction</p> <p>We present an interesting and unusual case of a 61-year-old woman with bilateral, undisplaced, stress neck of femur fractures associated with short-term steroid use. Insufficiency fractures of the neck of femur without preceding trauma have been described in the literature, although bilateral involvement is infrequent. These fractures have been associated with strenuous exercise, seizures, renal osteodystrophy, fluoride treatment, long-term corticosteroid use, amenorrhoea, abnormal anatomy and osteomalacia due to nutritional and/or hormonal factors.</p> <p>Case Presentation</p> <p>The case we present differs from other published reports, in that the patient's symptoms developed acutely after only a short course of steroids and with no associated trauma or strenuous exercise. It is also the only case described where no operative intervention was required.</p> <p>Conclusion</p> <p>Our case reiterates the importance of considering insufficiency or stress fractures in high-risk patients who present with musculoskeletal pain. Institution of bone protection should also be considered in these patients. Morbidity related to delayed treatment has been well documented, so a high level of clinical suspicion is imperative.</p

Saddle point localization of molecular wavefunctions

The quantum mechanical description of isomerization is based on bound eigenstates of the molecular potential energy surface. For the near-minimum regions there is a textbook-based relationship between the potential and eigenenergies. Here we show how the saddle point region that connects the two minima is encoded in the eigenstates of the model quartic potential and in the energy levels of the [H, C, N] potential energy surface. We model the spacing of the eigenenergies with the energy dependent classical oscillation frequency decreasing to zero at the saddle point. The eigenstates with the smallest spacing are localized at the saddle point. The analysis of the HCN???HNC isomerization states shows that the eigenstates with small energy spacing relative to the effective (v1, v3, l) bending potentials are highly localized in the bending coordinate at the transition state. These spectroscopically detectable states represent a chemical marker of the transition state in the eigenenergy spectrum. The method developed here provides a basis for modeling characteristic patterns in the eigenenergy spectrum of bound states

Endovascular treatment of thoracoabdominal aortic aneurysm: a case report

<p>Abstract</p> <p>Introduction</p> <p>Thoracoabdominal aortic aneurysms usually present in elderly patients with serious renal, pulmonary, cerebral, or cardiac comorbidities that pose a great challenge to the attending surgeon. Endovascular techniques for the treatment of thoracoabdominal aneurysms are not yet widely used due to limitations associated with them, such as spinal and visceral ischemia.</p> <p>Case presentation</p> <p>An 87-year-old Caucasian man with a symptomatic Crawford type I thoracoabdominal aortic aneurysm was treated successfully with a long tube stent graft using endovascular techniques and without any complication in follow-up examinations. The stent was placed distal to the left subclavian artery, and proximal to the celiac axis.</p> <p>Conclusion</p> <p>The use of endovascular stents for long segment thoracoabdominal aortic aneurysms needs to undergo clinical investigation to determine whether this procedure decreases morbidity and mortality rates.</p

Crafting History: How the World Is Made. The Case of Islamic Archaeology

In this paper an archaeological and theoretical perspective that builds a relationship between the concepts of craft and of identity is presented. Both of them are concepts very widely used in archaeological and anthropological theory nowadays, and they have often been linked in field studies. However, these concepts are usually contemplated from very different points of view and with many diverse implications in each case. One of the aims of this paper is to show that craft and identity can be inserted in a common theoretical framework which in turn can be used to understand cultural change or, in other words, history within culture. The paper will start with a necessary theoretical introduction to different concepts related to craft and identity, and then a discussion on how to link these different concepts will follow. In the last part of the paper, this theoretical perspective will be applied to a field which is familiar to the author, that of Islamic archaeology. A case example of the author’s research in the Vega of Granada (southeast Spain) will be brought to the fore. This part of the paper will show how the theoretical discussion developed above can contribute to solve one of the core questions of this field, that of the definition of an Islamic culture and its application to understand the daily life of people living within it