96 research outputs found
Epidemiology of the lymphatic-dwelling filarioid nematode Rumenfilaria andersoni in free-ranging moose (Alces alces) and other cervids of North America
Background: Moose (Alces alces) are a culturally and economically valued species in Minnesota, where the northeast population has decreased by 60 % since 2006. The cause of the decline is currently unclear; however, parasites, predation, and climate change have all been implicated. Nematode parasites are important pathogens in North American moose, potentially causing severe disease and mortality. Recent spread of Rumenfilaria andersoni, a filarioid nematode of moose, has been documented in Finnish cervids; however, little is known about the epidemiology of this parasite in North America. Methods: To investigate the prevalence and distribution of R. andersoni, 584 blood samples were collected from live-captured and dead animals and screened microscopically for the presence of microfilariae using a modified Knott's test. Microfilariae were identified based on morphological characteristics. A subset of Knott's-positive animals was subjected to polymerase chain reaction (PCR) with filarioid-specific primers targeting the first internal transcribed spacer region (ITS-1) of the rRNA gene cluster. Results: Rumenfilaria microfilariae were present in 20.5 % of Minnesota moose (n = 352), with slight fluctuations observed over four years. Minnesota white-tailed deer (Odocoileus virginianus) (n = 2) and moose (n = 44) from Alaska, Montana, Washington, Maine, and New Hampshire also harbored R. andersoni, suggesting this parasite occurs widely throughout North American moose herds, and white-tailed deer can serve as a patent host. Sequence analysis of cervid blood (moose, n = 15; white-tailed deer, n = 1) confirmed the identity of R. andersoni and revealed the existence of two distinct clades. Genetic comparisons of R. andersoni isolates from North America and semi-domesticated Finnish reindeer found the two groups to be closely related, supporting previous hypotheses that R. andersoni was recently introduced into Finland by the importation of deer from the United States. Conclusions: To the best of our knowledge these observations represent the first report of R. andersoni within the contiguous United States and reveal this nematode as a common parasite of North American moose and white-tailed deer. Although the implications of R. andersoni infection on moose health is unclear, increased awareness of this parasite will help prevent unintentional introduction of R. andersoni into naive populations via the translocation of wild and captive cervids.Peer reviewe
Differential responses of ammonia-oxidizing archaea and bacteria to long-term fertilization in a New England salt marsh
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Aquatic Microbiology 3 (2013): 445, doi:10.3389/fmicb.2012.00445.Since the discovery of ammonia-oxidizing archaea (AOA), new questions have arisen about population and community dynamics and potential interactions between AOA and ammonia-oxidizing bacteria (AOB). We investigated the effects of long-term fertilization on AOA and AOB in the Great Sippewissett Marsh, Falmouth, MA, USA to address some of these questions. Sediment samples were collected from low and high marsh habitats in July 2009 from replicate plots that received low (LF), high (HF), and extra high (XF) levels of a mixed NPK fertilizer biweekly during the growing season since 1974. Additional untreated plots were included as controls (C). Terminal restriction fragment length polymorphism analysis of the amoA genes revealed distinct shifts in AOB communities related to fertilization treatment, but the response patterns of AOA were less consistent. Four AOB operational taxonomic units (OTUs) predictably and significantly responded to fertilization, but only one AOA OTU showed a significant pattern. Betaproteobacterial amoA gene sequences within the Nitrosospira-like cluster dominated at C and LF sites, while sequences related to Nitrosomonas spp. dominated at HF and XF sites. We identified some clusters of AOA sequences recovered primarily from high fertilization regimes, but other clusters consisted of sequences recovered from all fertilization treatments, suggesting greater physiological diversity. Surprisingly, fertilization appeared to have little impact on abundance of AOA or AOB. In summary, our data reveal striking patterns for AOA and AOB in response to long-term fertilization, and also suggest a missing link between community composition and abundance and nitrogen processing in the marsh.This work was supported in part by the National Science Foundation award DEB-0814586 (to Anne E. Bernhard). Additional support was provided by the George and Carol Milne Endowment at Connecticut College
Force-controlled pose optimization and trajectory planning for chained Stewart platforms
Introduction: We study optimization methods for poses and movements of chained Stewart platforms (SPs) that we call an “Assembler” Robot. These chained SPs are parallel mechanisms that are stronger, stiffer, and more precise, on average, than their serial counterparts at the cost of a smaller range of motion. By linking these units in a series, their individual limitations are overcome while maintaining truss-like rigidity. This opens up potential uses in various applications, especially in complex space missions in conjunction with other robots.Methods: To enhance the efficiency and longevity of the Assembler Robot, we developed algorithms and optimization models. The main goal of these methodologies is to efficiently decide on favorable positions and movements that reduce force loads on the robot, consequently minimizing wear.Results: The optimized maneuvers of the interior plates of the Assembler result in more evenly distributed load forces through the legs of each constituent SP. This optimization allows for a larger workspace and a greater overall payload capacity. Our computations primarily focus on assemblers with four chained SPs.Discussion: Although our study primarily revolves around assemblers with four chained SPs, our methods are versatile and can be applied to an arbitrary number of SPs. Furthermore, these methodologies can be extended to general over-actuated truss-like robot architectures. The Assembler, designed to function collaboratively with several other robots, holds promise for a variety of space missions
Dense Gas Tracers in Perseus: Relating the N2H+, NH3, and Dust Continuum Properties of Pre- and Proto-Stellar Cores
We investigate 35 pre-stellar cores and 36 proto-stellar cores in the Perseus
molecular cloud. We find a very tight correlation between the physical
parameters describing the N2H+ and NH3 gas. Both the velocity centroids and the
line widths of N2H+ and NH3 correlate much better than either species
correlates with CO, as expected if the nitrogen-bearing species are probing
primarily the dense core gas where the CO has been depleted. We also find a
tight correlation in the inferred abundance ratio between N2H+ and para-NH3
across all cores, with N(p-NH3)/N(N2H+)= 22 +/- 10. We find a mild correlation
between NH3 (and N2H+) column density and the (sub)millimeter dust continuum
derived H2 column density for pre-stellar cores, N(p-NH3)/N(H2) ~ 10e-8, but do
not find a fixed ratio for proto-stellar cores.
The observations suggest that in the Perseus molecular cloud the formation
and destruction mechanisms for the two nitrogen-bearing species are similar,
regardless of the physical conditions in the dense core gas. While the
equivalence of N2H+ and NH3 as powerful tracers of dense gas is validated, the
lack of correspondence between these species and the (sub)millimeter dust
continuum observations for proto-stellar cores is disconcerting and presently
unexplained.Comment: ApJ accepted. 45 pages, 8 figure
Emergence of the arterial worm Elaeophora schneideri in moose (Alces alces) and tabanid fly vectors in northeastern Minnesota, USA
Background
Moose (Alces alces) are a culturally and economically valued species in Minnesota. However, the moose population has experienced a sudden, marked decline in their range, including extirpation in the northwest and a 66% decline in the last decade in the northeast portions of the state. Although the exact cause of this decline is unclear, parasitic metastrongylid and filarioid nematode infections are known causes of morbidity and mortality in moose across North America.
Methods
To determine if these parasitic nematodes could be contributing to the Minnesota moose population decline, we molecularly examined banked tissues obtained from moose that died of known and unknown causes for the presence of nematode DNA. Extracted brain DNA of 34 individual moose was amplified utilizing primers targeting the 18S rRNA gene and internal transcribed spacer regions of nematodes.
Results
DNA sequencing revealed that PCR products obtained from 15 (44.1%) of the moose were 99% identical to Parelaphostrongylus tenuis, a metastrongylid known to cause neurological disease and death. Additionally, brain tissue from 20 (58.8%) individuals yielded sequences that most closely aligned with Elaeophora schneideri, a parasite associated with neurological impairment but previously unreported in Minnesota. Setaria yehi, a common filarioid parasite of deer, was also detected in the brain tissue of 5 (14.7%) moose. Molecular screening of 618 captured tabanid flies from four trapping sites revealed E. schneideri was present (6%) in the Minnesota environment and transmission could occur locally. Prevalence rates among the flies ranged between 0–100% per trapping site, with Chrysops spp. and Hybomitra spp. implicated as the vectors.
Conclusions
Ultimately, these data confirm that P. tenuis is widespread in the Minnesota moose population and raises the question of the significance of E. schneideri as a contributing factor to morbidity and mortality in moose
Akzeptanz erneuerbarer Kraftstoffe
Um den Hochlauf von erneuerbaren Kraftstoffen abschätzen zu können, ist es wichtig die Akzeptanz in unterschiedlichen Einsatzgebeten zu untersuchen. Im Rahmen dieses Vortrags werden die Ergebnisse von Akzeptanzanalysen für alternative Kraftstoffe in den Bereichen Schifahrt, Luftfahrt und Straßenverkehr vorgestellt. Dabei zeigt sich, dass die Grundvoraussetzungen für die Akzeptanz von erneuerbaren Kraftstoffen eine nachhaltige Bereitstellung und eine nutzerfreundliche Integration in das Bestandssystem sind
A SEROSURVEY OF DISEASES OF FREE-RANGING GRAY WOLVES (\u3ci\u3eCANIS LUPUS\u3c/i\u3e) IN MINNESOTA, USA
We tested serum samples from 387 free-ranging wolves (Canis lupus) from 2007 to 2013 for exposure to eight canid pathogens to establish baseline data on disease prevalence and spatial distribution in Minnesota’s wolf population. We found high exposure to canine adenoviruses 1 and 2 (88% adults, 45% pups), canine parvovirus (82% adults, 24% pups), and Lyme disease (76% adults, 39% pups). Sixty-six percent of adults and 36% of pups exhibited exposure to the protozoan parasite Neospora caninum. Exposure to arboviruses was confirmed, including West Nile virus (37% adults, 18% pups) and eastern equine encephalitis (3% adults). Exposure rates were lower for canine distemper (19% adults, 5% pups) and heartworm (7% adults, 3% pups). Significant spatial trends were observed in wolves exposed to canine parvovirus and Lyme disease. Serologic data do not confirm clinical disease, but better understanding of disease ecology of wolves can provide valuable insight into wildlife population dynamics and improve management of these species
The Bolocam Galactic Plane Survey IV: 1.1 and 0.35 mm Dust Continuum Emission in the Galactic Center Region
The Bolocam Galactic Plane Survey (BGPS) data for a six square degree region
of the Galactic plane containing the Galactic center is analyzed and compared
to infrared and radio continuum data. The BGPS 1.1 mm emission consists of
clumps interconnected by a network of fainter filaments surrounding cavities, a
few of which are filled with diffuse near-IR emission indicating the presence
of warm dust or with radio continuum characteristic of HII regions or supernova
remnants. New 350 {\mu}m images of the environments of the two brightest
regions, Sgr A and B, are presented. Sgr B2 is the brightest mm-emitting clump
in the Central Molecular Zone and may be forming the closest analog to a super
star cluster in the Galaxy. The Central Molecular Zone (CMZ) contains the
highest concentration of mm and sub-mm emitting dense clumps in the Galaxy.
Most 1.1 mm features at positive longitudes are seen in silhouette against the
3.6 to 24 {\mu}m background observed by the Spitzer Space Telescope. However,
only a few clumps at negative longitudes are seen in absorption, confirming the
hypothesis that positive longitude clumps in the CMZ tend to be on the
near-side of the Galactic center, consistent with the suspected orientation of
the central bar in our Galaxy. Some 1.1 mm cloud surfaces are seen in emission
at 8 {\mu}m, presumably due to polycyclic aromatic hydrocarbons (PAHs). A
~0.2\degree (~30 pc) diameter cavity and infrared bubble between l \approx
0.0\degree and 0.2\degree surrounds the Arches and Quintuplet clusters and Sgr
A. The bubble contains several clumpy dust filaments that point toward Sgr
A\ast; its potential role in their formation is explored. [abstract truncated]Comment: 76 pages, 22 figures, published in ApJ:
http://iopscience.iop.org/0004-637X/721/1/137
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Enhanced Direct Major Histocompatibility Complex Class I Self-Antigen Presentation Induced by Chlamydia Infection
The direct major histocompatibility complex (MHC) class I antigen presentation pathway ensures intracellular peptides are displayed at the cellular surface for recognition of infected or transformed cells by CD8⁺ cytotoxic T lymphocytes. Chlamydia spp. are obligate intracellular bacteria and, as such, should be targeted by CD8⁺ T cells. It is likely that Chlamydia spp. have evolved mechanisms to avoid the CD8⁺ killer T cell responses by interfering with MHC class I antigen presentation. Using a model system of self-peptide presentation which allows for posttranslational control of the model protein's stability, we tested the ability of various Chlamydia species to alter direct MHC class I antigen presentation. Infection of the JY lymphoblastoid cell line limited the accumulation of a model host protein and increased presentation of the model-protein-derived peptides. Enhanced self-peptide presentation was detected only when presentation was restricted to defective ribosomal products, or DRiPs, and total MHC class I levels remained unaltered. Skewed antigen presentation was dependent on a bacterial synthesized component, as evidenced by reversal of the observed phenotype upon preventing bacterial transcription, translation, and the inhibition of bacterial lipooligosaccharide synthesis. These data suggest that Chlamydia spp. have evolved to alter the host antigen presentation machinery to favor presentation of defective and rapidly degraded forms of self-antigen, possibly as a mechanism to diminish the presentation of peptides derived from bacterial proteins
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