Utility of Masson’s Trichrome Stain in the Quantification of Mean Vascular Density in Normal Oral Mucosa, Epithelial Dysplasia and Oral Squamous Cell Carcinoma

Indexación: Scopus; Scielo.El objetivo de este estudio fue valuar la utilidad del uso de la tinción de Tricrómico de Masson (TM) en la cuantificación de la densidad media vascular (DMV) en Mucosa Oral Normal (MON), Displasia Epitelial Oral (DEO) y Carcinoma Oral de Células Escamosas (COCE). Estudio descriptivo de serie de casos. Se analizaron 17 muestras de MON, 15 muestras de DEO y 16 de COCE, teñidas con TM. Para determinar su utilidad, se compararon con las mismas muestras analizadas con técnica de inmunohistoquímica contra CD31. La cuantificación de la DMV se realizó en las 3 áreas de mayor vascularización de cada muestra. Se determinó la DMV según diagnóstico mediante la tinción TM e inmunohistoquímica contra CD31, y se calculó la correlación entre ambos. La DMV cuantificada con TM y contra CD31 difiere según el diagnóstico, observándose un aumento de la DMV al malignizarse el diagnóstico. No se encontraron diferencias al comparar la DMV cuantificada con TM y contra CD31. La correlación de la DMV analizado por TM y contra CD31 es significativa y moderada. La cuantificación de vasos sanguíneos es posible mediante la tinción de TM en muestras de MON, DEO y COCE, con una correlación moderada con la inmunohistoquímica contra CD31.The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000401576&lng=en&nrm=iso&tlng=e

Eficacia y seguridad del adhesivo tisular Tisuacryl en el cierre de la piel de la episiotomía

Se realizó un ensayo clínico fase III, en el Hospital Docente Materno Infantil 10 de Octubre, de Ciudad de La Habana, Cuba, entre julio de 1999 y marzo del 2000, con el objetivo de evaluar la eficacia y seguridad del adhesivo tisular Tisuacryl en el cierre de la piel de la episiotomía. Cada grupo constó de 300 pacientes, de acuerdo a los criterios de inclusión establecidos para el ensayo. En el grupo estudio se presentaron menos molestias en la episiorrafia (95.6 %) que en el grupo control (56.3 %) a los 30 minutos post parto, así como al tercer día posterior al nacimiento (97.6 % vs. 61.3 %). Al séptimo día, los resultados en el afrontamiento, estética y molestias en la herida fueron significativamente mejores con el adhesivo tisular evaluado. Los riesgos relativos de resultados adversos al séptimo día posterior al parto fueron 3.2 veces mayores en relación con afrontamiento y 2.9 veces con la estética no satisfactorias cuando se utilizó catgut cromado al comparar con el Tisuacryl. Para las molestias en la episiorrafia, el riesgo relativo se elevó a 24.2, todos con diferencias estadísticas significativas. Se concluye que el uso del adhesivo tisular Tisuacryl para el cierre de la piel en la episiotomía tiene menor probabilidad de afrontamiento y estética de la herida no satisfactoria y de menor sensación dolorosa en la herida quirúrgica que el catgut cromado.Peer Reviewe

Structure of the medium formed in heavy ion collisions

We investigate the structure of the medium formed in heavy ion collisions using three different models: the Color String Percolation Model (CSPM), the Core-Shell-Color String Percolation Model (CSCSPM), and the Color Glass Condensate (CGC) framework. We analyze the radial distribution function of the transverse representation of color flux tubes in each model to determine the medium's structure. Our results indicate that the CSPM behaves as an ideal gas, while the CSCSPM exhibits a structural phase transition from a gas-like to a liquid-like structure. Additionally, our analysis of the CGC framework suggests that it produces systems that behave like interacting gases for AuAu central collisions at RHIC energies and liquid-like structures for PbPb central collisions at LHC energies.Comment: 15 pages, 8 figure

A model for the biochemical degradation of inosine monophosphate in hake (Merluccius merluccius)

7 páginas, 3 tablas, 3 figuras, 1 apéndiceATP-derived products are typically used as early indicators of fish quality loss during storage. In this work, we explore different biochemical routes that are potentially relevant in contributing to nucleotide degradation in hake (Merluccius merluccius). A major motivation of this study is to get more insight on the biochemical degradation mechanisms of nucleotide catabolites in hake muscle at fish storage and transport conditions. This requires the identification of its relevant pathways. To that purpose, different degradation routes proposed in the literature are considered and a mathematical model for the degradation process is derived. First order kinetics are assumed for all the reactions and temperature dependence is taken into account through the Arrhenius equation. Unknown model parameters, namely activation energies and pre-exponential Arrhenius coefficients, are estimated via fitting to experimental data. From the estimation results, relevant routes are identified. The kinetic study is performed on sterile fish juice to avoid coupling with microbial degradation mechanisms or possible interferences of the food matrix that might hide biochemical interactions. The proposed scheme adequately describes biochemical changes in nucleotide catabolites under variable temperature profiles. It also reveals a pathway which at least seems relevant for nucleotide degradation in hakeThe authors acknowledge financial support from the Spanish Ministry of Science and Innovation (Projects ISFORQUALITY AGL2012-39951-C02-01, PIE 201230E042 and RESISTANCE DPI2014-54085-JIN)Peer reviewe

FACTORES DE RIESGOS QUIMICOS EN EL PERSONAL DE ENFERMERÍA.

In the present study the chemical risks to the ones were identified that the personnel is exposed of nursing. They were included in the study to 600 professionals of nursing, excluding al personal of conduction (Supervisors and Chief) and the ones that were found with sick leave. A risks evaluation headquarters was utilized where the chemical risks were registered. Al to carry out the sociodemographic analysis was found greater quantity of personnel of the female sex and without university training. It was determined associated pathologies to the chemical risks, relating to the exposition and with the control measures lack. It was found significance statistical that would be able us to indicate that the pathologies that declare the polled would be able to be related to the exposition.En el presente estudio se identificaron los riesgos químicos a los que se expone el personal de enfermería. Fueron incluidos en el estudio 600 profesionales de enfermería, excluyendo al personal de conducción (Supervisores y Jefes) y los que se encontraban con licencia por enfermedad. Se utilizó una matriz de evaluación de riesgos donde se registraron los riesgos químicos. Al realizar el análisis sociodemográfico se encontró mayor cantidad de personal del sexo femenino y sin capacitación universitaria. Se determinaron patologías asociadas a los riesgos químicos, relacionándolas con la exposición y con la falta de medidas de control. El análisis estadístico de los resultados indicaría que las patologías que manifiestan los encuestados podrían estar relacionadas con la exposición a agentes químicos

Assessing multidomain overlaps and grand nnsemble generation in CORDEX regional projections

ABSTRACT: The Coordinated Regional Climate Downscaling Experiment (CORDEX) initiative has made available an enormous amount of regional climate projections in different domains worldwide. This information is crucial for the development of adaptation strategies and policy-making. A relevant open issue in this context is assessing the potential multidomain conflicts that may result in overlapping regions and developing appropriate ensemble methods trying to make the most of all available information. This work addresses this timely topic by focusing on precipitation over the Mediterranean region, a first illustrative case study that is encompassed by both the Euro- and Africa-CORDEX domains. We focus on several mean, extreme, and temporal indices and use variance decomposition to assess the separate contribution of the domain and models to the climate change signal, concluding that the contribution of the domain alone is nearly negligible (below urn:x-wiley:grl:media:grl60267:grl60267-math-0001 in all cases). Nevertheless, for some cases, the combined model/domain effect triggers up to urn:x-wiley:grl:media:grl60267:grl60267-math-0002 of the total variance.This work has been funded by the Spanish R+D Program of the Ministry of Economy and Competitiveness, through projects MULTI-SDM (CGL2015-66583-R) and INSIGNIA (CGL2016-79210-R), cofunded by the European Regional Development Fund (ERDF/FEDER)

First report of Tomato torrado virus on weed hosts in Spain

Alfaro Fernández, AO.; Córdoba-Sellés, C.; Cebrián, M.; Herrera-Vásquez, J.; Sanchez Navarro, JA.; Juárez, M.; Espino, A.... (2088). First report of Tomato torrado virus on weed hosts in Spain. Plant Disease. 92(5):831-831. https://doi.org/10.1094/pdis-92-5-0831b83183192

Application of multi-sensor advanced DInSAR analysis to severe land subsidence recognition: Alto Guadalentín Basin (Spain)

Multi-sensor advanced DInSAR analyses have been performed and compared with two GPS station measurements, in order to evaluate the land subsidence evolution in a 20-year period, in the Alto Guadalentín Basin where the highest rate of man-induced subsidence (> 10 cm yr−1) of Europe had been detected. The control mechanisms have been examined comparing the advanced DInSAR data with conditioning and triggering factors (i.e. isobaths of Plio-Quaternary deposits, soft soil thickness and piezometric level)