Supplementary Tables S7-S9

Supplementary Table S7 - Porcine plasma proteins submitted for further analysis.Supplementary Table S8 - Proteins significantly altered in response to the T1 diet.Supplementary Table S9 - Proteins significantly altered in response to the T2 diet

Supplementary Tables S9-S11

Supplementary Table S9 - Porcine plasma proteins submitted for further analysis.Supplementary Table S10 - Proteins significantly altered in response to the T1 diet.Supplementary Table S11 - Proteins significantly altered in response to the T2 diet.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Supplementary Tables S9-S11

Supplementary Table S9 - Porcine plasma proteins submitted for further analysis.Supplementary Table S10 - Proteins significantly altered in response to the T1 diet.Supplementary Table S11 - Proteins significantly altered in response to the T2 diet

Regulacja bilansu chlorków w osoczu krwi cieląt w pierwszym tygodniu życia

Badania przeprowadzono na 10 klinicznie zdrowych cieliczkach rasy polsko-fryzyjskiej odmiany czarno-białej, w ciągu pierwszych 7 dnia życia postnatalnego. Celem prezentowanych badań była analiza nerkowej regulacji bilansu chlorków podczas pierwszego tygodnia życia cieląt. Stężenie chlorków w osoczu krwi cieląt w pierwszym tygodniu życia wynosiło średnio 98,86 mmol · l–1 i mieściło się w granicach norm fizjologicznych. Do piątego dnia życia koncentracja tego elektrolitu była stabilna, a następnie w siódmym dniu życia nieznacznie się obniżyła. Przy ujednoliconym żywieniu (siarą i mlekiem matek) obserwowane zmiany w kolejnych dniach pierwszego tygodnia życia związane były głównie ze zmianami czynności nerek (przesączania w kłębkach i resorpcji kanalikowej). W pierwszym tygodniu życia cieląt obserwowano istotne zmiany wielkości ładunku przesączonego, resorpcji kanalikowej oraz wydalania chlorków z moczem. Analiza zmian wielkości ładunku przesączonego chlorków wskazuje, że zmiana stężenia chlorków w osoczu krwi nie miała wpływu na ten wskaźnik. Stwierdzono, że decydującym mechanizmem regulującym wydalanie chlorków z moczem są zmiany wielkości resorpcji tego elektrolitu w kanalikach nerkowych. Uzyskane wyniki wskazują, że nerki cieląt noworodków są dostatecznie przygotowane do regulacji stężenia chlorków w osoczu krwi.The experiment was carried out on 10 clinically healthy, female Polish-Friesian var. Black-and-White calves, during the first seven days of postnatal life. The aim of this study was to analyze renal regulation of chloride balance in calves during the first seven days of life. The mean blood plasma chloride concentration during the first week of calves’ life was 98.86 mmol · l–1 and was within the physiological reference values. The concentration of this electrolyte was stable until the fifth day of life, and was followed by a slight decrease on the seventh day of life. Due to the fact that the feeding was standardized (dam’s colostrum and milk), the changes in concentration of plasma chloride in the following days of the first week of life were mainly associated with the renal activity (glomerular filtration and tubular reabsorption). Significant changes in glomerular filtration load, tubular reabsorption and urine excretion of chloride were recorded during the first week of life. The values of the filtered load of chloride were not influenced by changes in plasma chloride concentration. Our results indicate that changes in chloride tubular reabsorption represent crucial mechanism responsible for urinary chloride excretion. The obtained results show that neonate calf’ kidneys are sufficiently prepared to regulate blood chloride level

Supplementary Tables S7-S9

Supplementary Table S7 - Porcine plasma proteins submitted for further analysis.Supplementary Table S8 - Proteins significantly altered in response to the T1 diet.Supplementary Table S9 - Proteins significantly altered in response to the T2 diet.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Feeding milk replacer instead of whole milk affects blood plasma proteome and lipid profile in preruminant calves

The study was undertaken to determine the effect of feeding milk or milk-replacer on the blood plasma proteome and lipid profile in calves during the second week of life. Feeding milk-replacer significantly decreased the expression of plasma apoA-I. Age of calves affected apoA-I expression, which was higher on the 8th than on the 11th and 14th day of life. A significant effect of interaction between diet and age was also observed. The expression of apoA-IV, was significantly affected by diet and was lower in calves fed milk replacer. Expression of this protein was significantly lower at the 8th day of life and was up-regulated in the calves fed milk-replacer at the second week of life. Calves fed milk-replacer had greater expression of haptoglobin, which differed significantly between days of blood sampling, being higher on the 8th than on the11th and 14th day. The interactive effect of diet and age affected haptoglobin expression, which was successively down-regulated in calves fed milk replacer. Diet had a significant effect on the plasma lipid profile. Animals fed milk had a greater concentration of TC, HDLC and LDLC. The composition of milk-replacer, especially fat source, is probably the main factor that affects expression of proteins involved in cholesterol metabolism and level of components of lipid profile in calves fed formula. We claim that the initially increased level of haptoglobin, followed by its decrease during the second week of life in calves fed milk-replacer may indicate the presence of short-term stress induced by changes in the feeding system

Proteomic strategies in research on the cardiovascular system

The evolution of proteomics facilitates protein separation, identification and characterization, which give better insight into molecular mechanisms underlying cardiovascular physiology and pathophysiology. Investigations on the expression of proteins, their interactions and post-translational modifications contributes to our better understanding of disease processes and provides new cardiovascular biomarkers. Up to now, laboratory animals have been employed in proteomic research on human disorders, although rodent models do not genuinely reflect human conditions. Because of the similarities in anatomy, physiology and metabolism, farm animals such as pigs, cows or sheep are increasingly being used as model organisms in human cardiovascular research. It should be noted that application of proteomics has a huge potential for gaining some new insight into physiology and pathophysiology of the cardiovascular system, which cannot be provided with conventional methods. Up to date, complete heart and aorta proteomes of human and several animal species have been established. Several proteomic studies on human diseases, including atherosclerosis, myocardial infarction and dilated cardiomyopathy, have been conducted and proved to be very valuable in bringing key information on their aetiology and progression, as well as new challenges for biomedical investigation. The aim of this review is to summarize achievements in proteomics of cardiovascular physiology and pathophysiology with the use of domestic and laboratory animal models

Excessive amount of lactose in the diet of two-week-old calves induces urinary protein changes

The present paper was undertaken to analyse and identify urinary proteins that were significantly altered in urine of calves in response to short-term administration of milk replacer with lactose addition. We used 2-D electrophoresis combined with matrix-assisted laser desorption/ionisation and time-of-flight (MALDI-TOF) mass spectrometry. Of all spots analysed, four showed significantly decreased abundance: alpha-1-antiproteinase (A1AT), serotransferrin (TF), sex hormone-binding globulin (SHBG) and cytochrome P450 2E1 (CYP2E1). One displayed an increased abundance: adenosine triphosphate (ATP)-citrate synthase. The changes in abundance of SHBG and CYP2E1 proteins were caused by the direct effect of an oversupply of sugar, while A1AT, TF and ATP-citrate synthase showed altered abundance probably due to indirect effects. The results of this study confirmed that calves' urine is a very precious biological material to evaluate the renal function, and it may be valuable in veterinary and zootechnical diagnostics