Characterization of TrxC, an Atypical Thioredoxin Exclusively Present in Cyanobacteria

Cyanobacteria form a diverse group of oxygenic photosynthetic prokaryotes considered to be the antecessor of plant chloroplast. They contain four different thioredoxins isoforms, three of them corresponding to m, x and y type present in plant chloroplast, while the fourth one (named TrxC) is exclusively found in cyanobacteria. TrxC has a modified active site (WCGLC) instead of the canonical (WCGPC) present in most thioredoxins. We have purified it and assayed its activity but surprisingly TrxC lacked all the classical activities, such as insulin precipitation or activation of the fructose-1,6-bisphosphatase. Mutants lacking trxC or over-expressing it were generated in the model cyanobacterium Synechocystis sp. PCC 6803 and their phenotypes have been analyzed. The ΔtrxC mutant grew at similar rates to WT in all conditions tested although it showed an increased carotenoid content especially under low carbon conditions. Overexpression strains showed reduced growth under the same conditions and accumulated lower amounts of carotenoids. They also showed lower oxygen evolution rates at high light but higher Fv’/Fm’ and Non-photochemical-quenching (NPQ) in dark adapted cells, suggesting a more oxidized plastoquinone pool. All these data suggest that TrxC might have a role in regulating photosynthetic adaptation to low carbon and/or high light conditions.España, MINECO BIO2016-75634-PJunta de Andalucía P12-BIO-1119 , BIO-28

Some mathematical principles of milityary combat and counter – insurgency

El trabajo comprende el uso de teoría matemática de sistemas dinámicos, teoría de estabilidad lineal y análisis no lineal para la construcción de modelos matemáticos de distintos escenarios de combate entre una fuerza militar y fuerzas insurgentes. El estudio y la interpretación de dichos modelos permiten a una fuerza militar tomar las decisiones óptimas para poder enfrentar de manera efectiva a movimientos insurgentes y evitar riesgos innecesarios.Tesi

Comparative Analysis for Web Applications Based on REST Services: MEAN Stack and Java EE Stack

The use of REST (Representational State Transfer Protocol) web services has increased and has become the most widely used implementation today. The most crucial part of a web development project based on REST services is the choice of the right tools for the front-end, back-end, and database environment. The main objective of this work is to test the MEAN stack as an effective solution for a web application based on REST services. JavaScript has been the client-side programming language running in the browser. The MEAN stack consists of MongoDB (database), Node.js. (web server), Express (back-end), and Angular (front-end) to build web applications. We compare the MEAN stack with a very popular stack such as Java EE that groups MongoDB (database), Apache/Tomcat (web server), Spring boot (back-end) and JSP/HTML/CSS (front-end), with respect to its components, features, and environment configuration. Two similar applications were built with the MEAN and Java EE stacks. We compared and analyzed several features and test criteria such as response time, performance, and data loading.     Keywords: software architecture, Javascript, REST, web service, Java E

Ordovician conodonts of the Devendeus Formation at the Angosto de La Quesera (Cordillera Oriental of Salta, Argentina) : Taxonomic considerations and biostratigraphic significance

Conodonts recovered from the Devendeus Formation at the Angosto de La Quesera locality (Cordillera Oriental of Salta) in northwestern Argentina include Nogamiconus sp., Problematoconites perforatus, Drepanoistodus sp., Oneotodus sp., Scolopodus filosus, Teridontus nakamurai and Variabiloconus sp., as a part of an association that defines a pre-Paltodus deltifer Zone. The material most likely represents the Cordylodus angulatus and/or the lower part of the Rossodus manitouensis Zone. These microfossils support the dating of the Devendeus Formation as Tremadocian (Early Ordovician)

Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in chlamydomonas

Fatty acids are synthesized in the stroma of plant and algal chloroplasts by the fatty acid synthase complex. Newly synthesized fatty acids are then used to generate plastidial lipids that are essential for chloroplast structure and function. Here, we show that inhibition of fatty acid synthesis in the model alga Chlamydomonas reinhardtii activates autophagy, a highly conserved catabolic process by which cells degrade intracellular material under adverse conditions to maintain cell homeostasis. Treatment of Chlamydomonas cells with cerulenin, a specific fatty acid synthase inhibitor, stimulated lipidation of the autophagosome protein ATG8 and enhanced autophagic flux. We found that inhibition of fatty acid synthesis decreased monogalactosyldiacylglycerol abundance, increased lutein content, down-regulated photosynthesis, and increased the production of reactive oxygen species. Electron microscopy revealed a high degree of thylakoid membrane stacking in cerulenin-treated cells. Moreover, global transcriptomic analysis of these cells showed an up-regulation of genes encoding chloroplast proteins involved in protein folding and oxidative stress and the induction of major catabolic processes, including autophagy and proteasome pathways. Thus, our results uncovered a link between lipid metabolism, chloroplast integrity, and autophagy through a mechanism that involves the activation of a chloroplast quality control system.Ministerio de Economía y Competitividad BFU2015-68216-PJunta de Andalucía CVI-7336, BIO2015-74432-JI

Modelo dinámico-probabilístico sobre el comportamiento de los pueblos indígenas en aislamiento de la Amazonia Ecuatoriana.

La supervivencia de los pueblos en aislamiento (PIAs), Tagaeri? Taromenane en la Amazonia ecuatoriana, está influida por agentes externos y características culturales. El área definida como Zona Intangible para el desplazamiento de los PIAs presenta cierta debilidad geográfica, lo que alterna el funcionamiento de estas comunidades. El objetivo de este trabajo es conocer el comportamiento de las comunidades aisladas en relación con las fronteras de contactos. Se ha utilizado el software libre NetLogo versión 5.0.3, para simular la evolución durante 100 años de dos comunidades. Las poblaciones estimadas iniciales de indígenas en asilamiento fue de 100, 500, 1000 individuos por escenario. El comportamiento está condicionado por las variables: individuos Taromenane, individuos Tagaeri, tasa de nacimientos, disponibilidad de alimento en la Amazonía, crecimiento de la vegetación, valor energético de la alimentación, y energía de la vegetación. Las características guerreras por la protección de territorio propias de los PIAs, han permitido concluir que la intensificación de las presiones externas sumadas a los enfrenamientos entre grupos aislados genera su propia extinción en un espacio temporal de 100 años