Effects of Electricity and Altered Conductivity on Rainbow Trout Embryos: A Study to Determine Efficacy of Electricity for Eradication of Invasive Salmonids

Electricity has been an applied means of facilitating capture and removal of invasive fishes for many years. Current methods involve use of electrodes to establish a current through which passing fish will be susceptible to a brief shock to stun. This method, however, only affects free swimming individuals and is not inclusive of early life history stages such as embryos within spawning substrate. This study evaluates the susceptibility of embryonic and larval stage rainbow trout (Oncorhynchus mykiss) to direct DC current between 2-20v/cm in varying conductive waters to determine lethality for invasive salmonid eradication efforts. Rainbow trout embryos (n = 10 embryos/exposure) were initially exposed to homogeneous electric fields for 5 sec with a water conductivity of 220uS/cm from 1 day post fertilization (DPF)/ 27 temperature units (TU) to 15 DPF/405TU. Mortality was assessed 24 hours post exposure and the LV50 (lethal voltage) at 220uS/cm was determined for each TU. Embryos from six periods of development were then exposed to their respective LV50 voltages in varying conductive waters (20-600uS/cm). Susceptibility to direct DC voltages increased with voltage but overall susceptibility decreased with development. Susceptibility to a constant voltage increased with increasing conductivity and was consistent throughout early development (81TU-292TU), but the effects of increased conductivity were not enhanced in eyed embryos after 364TU. Results indicate that direct DC current applied prior to eyed embryonic stages, the period of greatest trout embryo susceptibility, is an effective means of eradicating invasive and nuisance salmonids

FATORES SOCIOCULTURAIS QUE INFLUENCIAM A PRÁTICA DA AMAMENTAÇAO ENTRE MULHERES DE BAIXA RENDA EM FORTALEZA, CEARÁ, BRASIL: UMA PERSPECTIVA A PARTIR DO MODELO DO SOL NASCENTE DE LEININGER

This study was developed to analyze socio-cultural factors that may influence the breastfeeding practices of low-income women in Fortaleza, Ceará, Brazil. This observational study utilized Leininger’s Culture Care Theory to identify and analyze key socio-cultural factors. The study was based on 12 pregnant and breastfeeding women. Using an observational data sheet created in concordance with the Sunrise model (Leininger), we visited the homes of pregnant and breastfeeding women and observed their living conditions. Our observations were recorded and photographs were taken of the overall housing conditions, the surrounding neighborhood, and the local clinic. Living conditions were impoverished. Most homes had inadequate refrigeration, structural problems, and provided small living space. This indicated the severity of the residents’ economic situations. The women observed were usually self-employed and living with at least one family member. The factors that appeared to have the most influence on women’s decisions regarding breastfeeding were familial and economic factors. It is expected that the findings of this study will lead to more culturally appropriate and effective interventions aimed at increasing breastfeeding initiation and duration.Esta investigación fue desarrollada para analizar los factores socioculturales que pueden influir en la práctica de la lactancia entre mujeres de baja renta en Fortaleza, Ceará, Brasil. Este estudio de observación utilizó la Teoría del Cuidado Cultural de Leininger para identificar y analizar los factores socioculturales clave. El estudio involucró a 12 gestantes y nodrizas. Usando un formulario de observación creado de acuerdo con el modelo del Sol Naciente (Leininger), fueron llevadas a cabo visitas en los domicilios de las mujeres y observadas sus condiciones de vida. Las observaciones fueron registradas en el formulario y se sacaron fotografías de las condiciones generales de la morada, de la vecindad y del servicio de salud local. Las condiciones de vida son pobres. La mayoría de las casas tiene refrigeración inadecuada, problemas estructurales y poco espacio. Eso revela la gravedad de las condiciones económicas de los residentes. Las mujeres observadas en general eran autónomas y vivían con por lo menos un miembro de la familia. Los factores que aparentemente ejercían mayor influencia sobre la decisión de las mujeres a favor de la lactancia fueron los económicos y familiares. Se espera que los hallazgos de este estudio lleven a intervenciones más efectivas y culturalmente apropiadas con objeto de aumentar el inicio y la duración de la lactancia.Este estudo foi desenvolvido para analisar os fatores socioculturais que podem influenciar a prática da amamentação entre mulheres de baixa renda em Fortaleza, Ceará, Brasil. Este estudo observacional utilizou a Teoria do Cuidado Cultural de Leininger para identificar e analisar os fatores socioculturais chave. O estudo envolveu 12 gestantes e nutrizes. Usando um formulário de observação criado de acordo com o modelo do Sol Nascente (Leininger) foram realizadas visitas nos domicílios das mulheres e observadas suas condições de vida. As observações foram registradas no formulário e foram realizadas fotografias das condições gerais da moradia, da vizinhança e do serviço de saúde local. As condições de vida são pobres. A maioria das casas tem refrigeração inadequada, problemas estruturais e pouco espaço. Isso revela a gravidade das condições econômicas dos residentes. As mulheres observadas em geral eram autônomas e viviam com pelo menos um membro da família. Os fatores que pareceram ter mais influência sobre a decisão das mulheres em amamentar foram os fatores econômicos e familiares. Espera-se que os achados deste estudo levem a intervenções mais efetivas e culturalmente apropriadas visando o aumento do início e duração da amamentação

The Photo Essay: A Visual Research Method for Educating Obstetricians and Other Health Care Professionals

When it comes to issues related to low-income women seeking early, adequate, or continuous prenatal ca re, the public health and medical communities continue to tell women to take responsibility for their actions. Rarely are messages aimed at providers. To help physicians see how factors in their offices and clinics can affect service utilization, the photo essay, a visual qualitative research strategy was developed using low- income minority and disenfranchised women who had recently given birth or were near to giving birth. Eight photo essays were completed. Together, the narratives, in collaboration with the photos, provided an opportunity for physicians to hear and observe women, as consumers, as they expanded their descriptions of their prenatal care experience

Predictors and associations with outcomes of length of hospital stay in patients with acute heart failure: results from VERITAS

Background: The length of hospital stay (LOS) is important in patients admitted for acute heart failure (AHF) because it prolongs an unpleasant experience for the patients and adds substantially to health care costs. Methods and Results: We examined the association between LOS and baseline characteristics, 10-day post-discharge HF readmission, and 90-day post-discharge mortality in 1347 patients with AHF enrolled in the VERITAS program. Longer LOS was associated with greater HF severity and disease burden at baseline; however, most of the variability of LOS could not be explained by these factors. LOS was associated with a higher HF risk of both HF readmission (odds ratio for 1-day increase: 1.08; 95% confidence interval [CI] 1.01–1.16; P = .019) and 90-day mortality (hazard ratio for 1-day increase: 1.05; 95% CI 1.02–1.07; P < .001), although these associations are partially explained by concurrent end-organ damage and worsening heart failure during the first days of admission. Conclusions: In patients who have been admitted for AHF, longer length of hospital stay is associated with a higher rate of short-term mortality. Clinical Trial Registration: VERITAS-1 and -2: Clinicaltrials.gov identifiers NCT00525707 and NCT00524433

Challenges of stroke management in resource-limited settings: A case- based reflection

A 19-year-old man presented with a 1-year history of headache, generalised body weakness, progressive memory loss, and disorientation. One month prior to admission, there was aggravation of the weakness of the right upper limb, with new-onset difficulty with mastication, speech impairment, apathy, and urinary incontinence. On clinical examination, the patient had a motor aphasia and a right-sided hemiparesis with increased muscle tone and hyperreflexia. A noncontrast computed tomography (CT) scan of the brain revealed large ischaemic strokes extending beyond the classical vascular territories. Cerebrospinal fluid analysis showed a mildly increased protein level. The electrocardiogram revealed an irregular sinus bradycardia. The remainder of the cardiovascular and laboratory workup was unremarkable. Considering a working diagnosis of central nervous system vasculitis, the patient was treated with aspirin, prednisolone, and physiotherapy. However, he died suddenly a few weeks later. Based on this case, we discuss the challenges of stroke management in resource-limited settings, provide practical tips for general practitioners, reflect on the potential avenues for short- and long-term action, and introduce the budding collaboration platform between the University College London, the University of Liverpool, the Queen Elizabeth Central Hospital, and the Malawi-Liverpool-Wellcome Trust Clinical Research Programme

Super-Enhancer-Associated LncRNA UCA1 Interacts Directly with AMOT to Activate YAP Target Genes in Epithelial Ovarian Cancer

Long noncoding RNAs (lncRNAs) have emerged as critical regulators of tumorigenesis, and yet their mechanistic roles remain challenging to characterize. Here, we integrate functional proteomics with lncRNA-interactome profiling to characterize Urothelial Cancer Associated 1 (UCA1), a candidate driver of ovarian cancer development. Reverse phase protein array (RPPA) analysis indicates that UCA1 activates transcription coactivator YAP and its target genes. In vivo RNA antisense purification (iRAP) of UCA1 interacting proteins identified angiomotin (AMOT), a known YAP regulator, as a direct binding partner. Loss-of-function experiments show that AMOT mediates YAP activation by UCA1, as UCA1 enhances the AMOT-YAP interaction to promote YAP dephosphorylation and nuclear translocation. Together, we characterize UCA1 as a lncRNA regulator of Hippo-YAP signaling and highlight the UCA1-AMOT-YAP signaling axis in ovarian cancer development

Designing a suite of measurements to understand the critical zone

Many scientists have begun to refer to the earth surface environment from the upper canopy to the depths of bedrock as the critical zone (CZ). Identification of the CZ as an integral object worthy of study implicitly posits that the study of the whole earth surface will provide benefits that do not arise when studying the individual parts. To study the CZ, however, requires prioritizing among the measurements that can be made – and we do not generally agree on the priorities. Currently, the Susquehanna Shale Hills Critical Zone Observatory (SSHCZO) is expanding from a small original focus area (0.08 km2 , Shale Hills catchment), to a larger watershed (164 km2 , Shavers Creek watershed) and is grappling with the prioritization. This effort is an expansion from a monolithologic first-order forested catchment to a watershed that encompasses several lithologies (shale, sandstone, limestone) and land use types (forest, agriculture). The goal of the project remains the same: to understand water, energy, gas, solute, and sediment (WEGSS) fluxes that are occurring today in the context of the record of those fluxes over geologic time as recorded in soil profiles, the sedimentary record, and landscape morphology. Given the small size of the Shale Hills catchment, the original design incorporated measurement of as many parameters as possible at high temporal and spatial density. In the larger Shavers Creek watershed, however, we must focus the measurements. We describe a strategy of data collection and modeling based on a geomorphological and land use framework that builds on the hillslope as the basic unit. Interpolation and extrapolation beyond specific sites relies on geophysical surveying, remote sensing, geomorphic analysis, the study of natural integrators such as streams, groundwaters or air, and application of a suite of CZ models. We hypothesize that measurements of a few important variables at strategic locations within a geomorphological framework will allow development of predictive models of CZ behavior. In turn, the measurements and models will reveal how the larger watershed will respond to perturbations both now and into the future