Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii

Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period of 12 months when stored at −70°C using dimethyl sulfoxide (DMSO; 5 or 10%) as cryopreservant. As A. castellanii are naturally capable of encystment, studies were undertaken to determine whether induced encystment might improve the viability of organisms under a number of storage conditions. A. castellanii cysts stored in the presence of Mg2+ at 4°C remained viable over the study period, although time to confluence was increased from approximately 8 days to approximately 24 days over the 12-month period. Storage of cysts at −70°C with DMSO (5 or 10%) or 40% glycerol, but not 80% glycerol as cryopreservants increased their viability over the 12-month study period compared with those stored at room temperature. Continued presence of Mg2+ in medium during storage had no adverse effects and generally improved recovery of viable organisms. The present study demonstrates that A. castellanii can be stored as a non-multiplicative form inexpensively, without a need for cryopreservation, for at least 12 months, but viability is increased by storage at −70°C

Preferencias hacia carne de cordero en supermercados de Temuco, región de la Araucanía, Chile

Lobos, G (Lobos, German). Univ Talca, Escuela Ingn Comercial, Talca, ChileThe low levels of consumption of lamb meat in Chile suggest the need for studies on consumer preferences in order to orient production. A study with this object was carried out to evaluate preferences for various cuts, breeds, state (fresh or frozen) and price of lamb's meat among supermarket buyers in Temuco, Chile, and the existence of different market segments, through a survey of 400 persons. Using a fractional factorial design for conjoint analysis, it was determined that the state of the meat was more important than the cut, the price and the breed, with a preference for meat in half carcasses and quarters, Araucano lamb, fresh, at a medium price level. Four consumer segments were distinguished by analysis of hierarchic conglomerates. The majority group (47.7%) was sensitive to the state and the cut, with preference for meat in quarters, fresh, Texel breed. The second group (25.3%) was sensitive to the breed, presenting the strongest preferences for meat in whole carcass, fresh, Araucano lamb. The third group (14.3%) was sensitive to the price, preferred meat in quarters, fresh, Araucano lamb. The minority group (12.7%) was sensitive to the state and price, preferring meat in quarters, Texel breed, and was the only group which preferred frozen meat and would pay a higher price. The segments were distinguished by age, ethnic origin and satisfaction with food-related life. Thus the commercialisation strategy for lamb in supermarkets in Temuco should concentrate principally on the sale of fresh meat cut in quarter

Fusarium head blight detection, spikelet estimation, and severity assessment in wheat using 3D convolutional neural networks

Fusarium head blight (FHB) is one of the most significant diseases affecting wheat and other small grain cereals worldwide. The development of resistant varieties requires the laborious task of field and greenhouse phenotyping. The applications considered in this work are the automated detection of FHB disease symptoms expressed on a wheat plant, the automated estimation of the total number of spikelets and the total number of infected spikelets on a wheat head, and the automated assessment of the FHB severity in infected wheat. The data used to generate the results are 3-dimensional (3D) multispectral point clouds (PC), which are 3D collections of points - each associated with a red, green, blue (RGB), and near-infrared (NIR) measurement. Over 300 wheat plant images were collected using a multispectral 3D scanner, and the labelled UW-MRDC 3D wheat dataset was created. The data was used to develop novel and efficient 3D convolutional neural network (CNN) models for FHB detection, which achieved 100% accuracy. The influence of the multispectral information on performance was evaluated, and our results showed the dominance of the RGB channels over both the NIR and the NIR plus RGB channels combined. Furthermore, novel and efficient 3D CNNs were created to estimate the total number of spikelets and the total number of infected spikelets on a wheat head, and our best models achieved mean absolute errors (MAE) of 1.13 and 1.56, respectively. Moreover, 3D CNN models for FHB severity estimation were created, and our best model achieved 8.6 MAE. A linear regression analysis between the visual FHB severity assessment and the FHB severity predicted by our 3D CNN was performed, and the results showed a significant correlation between the two variables with a 0.0001 P-value and 0.94 R-squared

The acanthamoeba shikimate pathway has a unique molecular arrangement and is essential for aromatic amino acid biosynthesis

The shikimate pathway is the only known biosynthetic route for de novo synthesis of aromatic compounds. It is described as an ancient eukaryotic innovation that has been retained in a subset of eukaryotes, replaced in plants through the acquisition of the chloroplast, but lost in many including humans. Herein, we demonstrate that Acanthamoeba castellanii possesses the shikimate pathway by biochemical and a combination of bioinformatics and molecular biological methods. The growth of A. castellanii (Neff strain and a recently isolated clinical specimen, both T4 genotypes) is inhibited by glyphosate [N-(phosphonomethyl) glycine], an inhibitor of EPSP synthase and the addition of phenylalanine and tryptophan, which are dependent on the shikimate pathway, rescued A. castellanii from glyphosate indicating that glyphosate was specific in action. A. castellanii has a novel complement of shikimate pathway enzymes including unique gene fusions, two Type I and one Type II DAHP synthases (for which their likely sensitivities to feedback inhibition by phenylalanine, tyrosine and tryptophan has been modelled) and a canonical chorismate synthase. The shikimate pathway in A. castellanii therefore has a novel molecular arrangement, is required for amino acid biosynthesis and represents an attractive target for antimicrobials

A Numerical Model of an Acoustic Metamaterial Using the Boundary Element Method Including Viscous and Thermal Losses

[EN] In recent years, boundary element method (BEM) and finite element method (FEM) implementations of acoustics in fluids with viscous and thermal losses have been developed. They are based on the linearized Navier¿Stokes equations with no flow. In this paper, such models with acoustic losses are applied to an acoustic metamaterial. Metamaterials are structures formed by smaller, usually periodic, units showing remarkable physical properties when observed as a whole. Acoustic losses are relevant in metamaterials in the millimeter scale. In addition, their geometry is intricate and challenging for numerical implementation. The results are compared with existing measurements.The authors wish to thank Mads J. Herring Jensen, from the company COMSOL, for his support in setting up the FEM model of the metamaterial. J. Sanchez-Dehesa acknowledges the support by the Spanish Ministerio de Economia y Competitividad, and the European Union Fondo Europeo de Desarrollo Regional (FEDER) through Project No. TEC2014-53088-C3-1-R.Cutanda-Henriquez, V.; Andersen, PR.; Jensen, JS.; Juhl, PM.; Sánchez-Dehesa Moreno-Cid, J. (2017). A Numerical Model of an Acoustic Metamaterial Using the Boundary Element Method Including Viscous and Thermal Losses. Journal of Computational Acoustics. 25(4):1750006-1-1750006-11. doi:10.1142/S0218396X17500060S1750006-11750006-11254Craster, R. V., & Guenneau, S. (Eds.). (2013). Acoustic Metamaterials. Springer Series in Materials Science. doi:10.1007/978-94-007-4813-2Cummer, S. A., Christensen, J., & Alù, A. (2016). Controlling sound with acoustic metamaterials. Nature Reviews Materials, 1(3). doi:10.1038/natrevmats.2016.1Cutanda-Henríquez, V., & Juhl, P. M. (2013). An axisymmetric boundary element formulation of sound wave propagation in fluids including viscous and thermal losses. The Journal of the Acoustical Society of America, 134(5), 3409-3418. doi:10.1121/1.4823840Bruneau, M., Herzog, P., Kergomard, J., & Polack, J. D. (1989). General formulation of the dispersion equation in bounded visco-thermal fluid, and application to some simple geometries. Wave Motion, 11(5), 441-451. doi:10.1016/0165-2125(89)90018-8Kampinga, W. R., Wijnant, Y. H., & de Boer, A. (2010). Performance of Several Viscothermal Acoustic Finite Elements. Acta Acustica united with Acustica, 96(1), 115-124. doi:10.3813/aaa.918262Kampinga, W. R., Wijnant, Y. H., & de Boer, A. (2011). An Efficient Finite Element Model for Viscothermal Acoustics. Acta Acustica united with Acustica, 97(4), 618-631. doi:10.3813/aaa.918442BELTMAN, W. M. (1999). VISCOTHERMAL WAVE PROPAGATION INCLUDING ACOUSTO-ELASTIC INTERACTION, PART I: THEORY. Journal of Sound and Vibration, 227(3), 555-586. doi:10.1006/jsvi.1999.2355Graciá-Salgado, R., García-Chocano, V. M., Torrent, D., & Sánchez-Dehesa, J. (2013). Negative mass density andρ-near-zero quasi-two-dimensional metamaterials: Design and applications. Physical Review B, 88(22). doi:10.1103/physrevb.88.224305Homentcovschi, D., & Miles, R. N. (2011). An analytical-numerical method for determining the mechanical response of a condenser microphone. The Journal of the Acoustical Society of America, 130(6), 3698-3705. doi:10.1121/1.3652853Geuzaine, C., & Remacle, J.-F. (2009). Gmsh: A 3-D finite element mesh generator with built-in pre- and post-processing facilities. International Journal for Numerical Methods in Engineering, 79(11), 1309-1331. doi:10.1002/nme.257

A phase 3 multicenter, prospective, open-label efficacy and safety study of immune globulin (human) 10% caprylate/chromatography purified in patients with myasthenia gravis exacerbations

Background: Myasthenia gravis (MG) is an autoimmune disorder affecting neuromuscular transmission. Exacerbations may involve increasing bulbar weakness and/or sudden respiratory failure, both of which can be critically disabling. Management of MG exacerbations includes plasma exchange and intravenous immunoglobulin (IVIG); they are equally effective, but patients experience fewer side effects with IVIG. The objective of this study was to assess the efficacy and safety of immune globulin caprylate/chromatography purified (IGIV-C) in subjects with MG exacerbations. Methods: This prospective, open-label, non-controlled 28-day clinical trial was conducted in adults with MG Foundation of America class IVb or V status. Subjects received IGIV-C 2 g/kg over 2 consecutive days (1 g/kg/day) and were assessed for efficacy/safety on Days 7, 14, 21, and 28. The primary efficacy endpoint was the change from Baseline in quantitative MG (QMG) score to Day 14. Secondary endpoints of clinical response, Baseline to Day 14, included at least a 3-point decrease in QMG and MG Composite and a 2-point decrease in MG-activities of daily living (MG-ADL). Results: Forty-nine subjects enrolled. The change in QMG score at Day 14 was significant (p < 0.001) in the Evaluable (-6.4, n = 43) and Safety (-6.7, n = 49) populations. Among evaluable subjects, Day 14 response rates were 77, 86, and 88% for QMG, MG Composite, and MG-ADL, respectively. IGIV-C showed good tolerability with no serious adverse events. Conclusions: The results of this study show that IGIV-C was effective, safe, and well tolerated in the treatment of MG exacerbations

Systemic metabolic derangement, pulmonary effects, and insulin insufficiency following subchronic ozone exposure in rats

Acute ozone exposure induces a classical stress response with elevated circulating stress hormones along with changes in glucose, protein and lipid metabolism in rats, with similar alterations in ozone-exposed humans. These stress-mediated changes over time have been linked to insulin resistance. We hypothesized that acute ozone-induced stress response and metabolic impairment would persist during subchronic episodic exposure and induce peripheral insulin resistance. Male Wistar Kyoto rats were exposed to air or 0.25 ppm or 1.00 ppm ozone, 5 h/day, 3 consecutive days/week (wk) for 13 wks. Pulmonary, metabolic, insulin signaling and stress endpoints were determined immediately after 13 wk or following a 1 wk recovery period (13 wk + 1 wk recovery). We show that episodic ozone exposure is associated with persistent pulmonary injury and inflammation, fasting hyperglycemia, glucose intolerance, as well as, elevated circulating adrenaline and cholesterol when measured at 13 wk, however, these responses were largely reversible following a 1 wk recovery. Moreover, the increases noted acutely after ozone exposure in non-esterified fatty acids and branched chain amino acid levels were not apparent following a subchronic exposure. Neither peripheral or tissue specific insulin resistance nor increased hepatic gluconeogenesis were present after subchronic ozone exposure. Instead, long-term ozone exposure lowered circulating insulin and severely impaired glucose-stimulated beta-cell insulin secretion. Thus, our findings in young-adult rats provide potential insights into epidemiological studies that show a positive association between ozone exposures and type 1 diabetes. Ozone-induced beta-cell dysfunction may secondarily contribute to other tissue-specific metabolic alterations following chronic exposure due to impaired regulation of glucose, lipid, and protein metabolism

Psychophysical Olfactory Tests and Detection of COVID-19 in Patients With Sudden Onset Olfactory Dysfunction: A Prospective Study

Objective: To investigate the coronavirus disease 2019 (COVID-19) status of patients with initial sudden olfactory anosmia (ISOA) through nasopharyngeal swabs for reverse transcription–polymerase chain reaction (RT-PCR) analysis and to explore their olfactory dysfunctions with psychophysical olfactory evaluation. Methods: A total of 78 ISOA patients were recruited from April 6, 2020, to April 10, 2020, through a public call of University of Mons (Mons, Belgium). Patients benefited from nasopharyngeal swabs and fulfilled the patient-reported outcome questionnaire. Among them, 46 patients performed psychophysical olfactory evaluation using olfactory identification testing. Based on the duration of the ISOA, 2 groups of patients were compared: patients with olfactory dysfunction duration ≤12 days (group 1) and those with duration >12 days (group 2). Results: In group 1, 42 patients (87.5%) had a positive viral load determined by RT-PCR and 6 patients (12.5%) were negative. In group 2, 7 patients (23%) had a positive viral load and 23 patients (77%) were negative. The psychophysical olfactory evaluation reported that anosmia and hyposmia occurred in 24 (52%) and 11 (24%) patients, respectively. Eleven patients were normosmic. The viral load was significantly higher in patients of group 1 compared with those of group 2. Conclusions: Coronavirus disease 2019 was detected in a high proportion of ISOA patients, especially over the first 12 days of olfactory dysfunction. Anosmia is an important symptom to consider in the detection of COVID-19 infection

Mediterranean diet and depression

Objective: The adherence to a Mediterranean Dietary Pattern ensures an adequate intake of B vitamins and w-3 fatty acids. A protective role on depression has been suggested for both nutrients. Design: Cross-sectional analysis from the SUN (Seguimiento Universidad de Navarra) prospective cohort study. Data from 9670 participants (4211 men and 5459 women) were analised. Logistic regression analyses were fitted to assess the association between B-vitamins and w-3 fatty acids intake (quintiles) and the prevalence of depression. Results: Folate intake was inversely associated with depression prevalence among men, especially smokers. Among women, B12 vitamin intake was inversely associated with depression, especially among smokers and physically active women. No significant associations were observed for w-3 fatty acids intake. Conclusions: The adherence to a Mediterranean Dietary Pattern ensures an adequate intake of fruits, nuts, vegetables, cereals, legumes or fish, important sources of nutrients linked to depression prevention

“…4542 Miles from Home…”: Repositioning English Language Learners as Power Brokers and Teachers as Learners in the Study Abroad Context

This article provides an empirical context for the role that bi/multi-lingual children and families may play in supporting pre-service and in-service educators engaging difference through a literacy and language situated study abroad internship in Chile. Drawing on data over a 15-year longitudinal study of the program, the authors examine how students and parents navigate serving the role of teacher, whereas the teacher participants navigate a new role as a learner in a context where they, many for the first time, experience being language and cultural minorities