PROJECT PEMBUATAN MEDIA INTERNAL FISCOMM ( FISIP COMMUNITY )

Internal media of internal communication media within an organization or company. Internal media published for purposes of internal organization, especially public organizations. The goal for the internal public are informed about policies and activities implemented company. So the vision and mission can be conveyed best through internal media. Thus, internal media publishing is a communication activities to meet the interests of the two parties, organizations or corporate interests and the interests of public internal. The title of the internal media Fisip Undip is "FIScomm" which stands for Community Fisip which could have a meaning that is, a container or means consisting of a large family Fisip Undip that serves as a medium aspiration, input, opinions, information, entertainment, and certainly also related policies Fisip Undip, as well as information that is instructive and informative, fresh and up to date. Internal media FIScomm is an internal media published every three months. Powered by a design that is not monotonous, colourfull, design and artwork, namely the combination of the abstract artwork and photo illustration. The content of our magazine is 40% under the theme of the organization, 20% and 40% non-organization of entertainment. The content of our magazine are: Cover, non-commercial advertising, editorial notes, content (content), image branding, technology, education, reviews, top news, inspiring, information, culinary, fashion, inboxmail, articles, travel. In making the internal medium, the writer served as editor as well as reporters. An editor assigned to conduct the process of editing (editing), an opinion editorial, proposing and writing news / photos to be loaded, giving direction to all the editorial team of the news that will be loaded on every issue, directing and fostering reporter in search of news and catch up news sources , determine the appropriateness of a news, photos, and design for a publishing house, planning the content for each issue, develop, nurture, build a lobby with news sources. While the task is to find a reporter and news sources are assigned to interview the editor, write the interview. Internal media FIScomm still far from perfection, so we desperately need the criticism and constructive suggestions from readers

Pain Relief with Wet Cupping Theraphy in Rats is Mediated by Heat Shock Protein 70 and β-Endorphin

Background: Wet cupping therapy is a complementary therapy in pain management. The mechanism of this therapy, however, needs further elucidation. Cells injured by wet cupping therapy seem to stimulate the expression of heat shock protein 70 (HSP70). Its benefit in pain reduction could be mediated by the expression of ß-endorphin. This study aimed at determining the correlation between HSP70 and ß-endorphin after wet cupping therapy. Methods: Sixteen male Wistar rats were divided into control (CG; n=8) and treatment (TG; n=8) groups. The rats in both groups were injected with complete Freund’s adjuvant (CFA) at the footpad. In the TG, wet cupping therapy was done at the left and right paralumbar regions 48 hours after the CFA injection. Twenty-four hours after therapy, the hot plate test was done to assess pain threshold. Thereafter, immunohistochemistry from the skin subjected to wet cupping therapy was conducted for HSP70 and ß-endorphin. Results: The expression of HSP70 was significantly higher in the keratinocytes of the TG (20.25±3.53; P<0.001) than in the keratinocytes of the CG (10.50±2.44; P<0.001). The expression of ß-endorphin was significantly higher in the keratinocytes of the TG (22.37±3.52; P<0.001) than in the keratinocytes of the CG (5.12±1.72; P<0.001). The results also revealed a high correlation between HSP70 and ß-endorphin (β=0.864; P<0.001). Pain threshold after wet cupping therapy was significantly higher in the TG (22.81±6.34 s; P=0.003) than in the CG (11.78±3.56 s). Conclusions: The benefit of wet cupping therapy in terms of pain reduction in rats could be mediated by the expression of HSP70 and ß-endorphin

An in Vitro Antiosteoporotic Activity of 96% Ethanol Extract of Abelmoschus Manihot L. Medik Leaves Using Mc3t3-e1 Preosteoblast Cells

Osteoporosis is a disease characterized by low bone mass and structural deterioration of bone tissue. Estrogen deficiency causes loss of bone mineral density which causes osteoporosis. Phytoestrogen is a potential alternative of estrogen that can be used as Hormone Replacement Therapy on osteoporosis with minimum side effects. Many edible plants contain phytoestrogens that are believed to promote bone health. Abelmoschus manihot (L.) Medik has been known as a plant that is empirically used in the traditional medicine and has potency to prevent osteoporosis. The aim of this research is to determine whether the Abelmoschus manihot (L.) Medik leaves have the potential to increase bone formation in an in vitro assays using preosteoblast cell line MC3T3-E1. The results showed an increasing activity of Alkaline phosphatase using confocal laser scanning microscopy technique

Validation of the self-completed Cambridge-Hopkins questionnaire (CH-RLSq) for ascertainment of restless legs syndrome (RLS) in a population survey.

BACKGROUND AND PURPOSE: Epidemiological studies of restless legs syndrome (RLS) have been limited by lack of a well validated patient-completed diagnostic questionnaire that has a high enough specificity to provide a reasonable positive predictive value. Most of the currently used patient completed diagnostic questionnaires have neither been validated nor included items facilitating the differential diagnosis of RLS from conditions producing similar symptoms. The Cambridge-Hopkins diagnostic questionnaire for RLS (CH-RLSq) was developed with several iterations to include items covering the basic diagnostic features of RLS and to provide some basic differential diagnosis. This validation study sought to determine the sensitivity and specificity of the RLS diagnosis based on this questionnaire. PATIENTS AND METHODS: The CH-RLSq was completed by 2005 blood donors who were asked to consent to being contacted for a telephone diagnostic interview. A scoring criterion was established for ascertainment of RLS based on the clinical definition of the disorder and the exclusion of "mimic" conditions. A weighted sample (N=185) of all completed questionnaires was selected for expert clinical diagnosis of RLS using the validated Hopkins Telephone Diagnostic Interview (HDTI). The telephone interviewers were blinded to all questionnaire responses. RESULTS: A telephone diagnosis was obtained on 183 of the sample's 185 questionnaires. The questionnaire's normalized sensitivity and specificity were 87.2% and 94.4%, respectively, for RLS compared to not RLS. The positive predictive values in this sample were 85.5%. CONCLUSIONS: The Cambridge-Hopkins RLS questionnaire provides a reasonable level of sensitivity and specificity for ascertainment of RLS in population-based studies

RLS and blood donation.

BACKGROUND AND PURPOSE: The link between brain iron deficiency and RLS is now well established. In a related observation, several conditions that can deplete iron stores have been linked to increased probability of RLS. Blood donation has been linked to iron deficiency. It has thus been hypothesized that donating blood may be a risk factor for developing RLS. PATIENTS AND METHODS: Two thousand and five UK blood donors, ranging from first-time donors to some who had donated more than 70 times, completed the validated Cambridge-Hopkins RLS questionnaire (CH-RLSq) following their donation session. The questionnaire included a set of questions designed to diagnose RLS. The donors' histories of blood donations were determined both from self-report and from the National Blood Service database. RESULTS: A number of statistical models were constructed to determine whether the probability of RLS diagnosis was related to the history of blood donations. Controlling for age and sex, no evidence was found to suggest that a greater number or frequency of blood donations increased the risk of RLS. Even amongst sub-groups especially vulnerable to iron depletion through blood donation, such as vegetarians or low weight individuals, no evidence for an increased risk of RLS could be found. CONCLUSIONS: We found no evidence that the frequency or number of blood donations up to the UK maximum of three times a year would increase the risk of RLS

Comparison of the increase of both muscle strength and hypertrophy of biceps brachii muscle in strengthening exercise with low-intensity resistance training with and without the application of blood flow restriction and high-intensity resistance training.

Background: Strengthening exercise is an important component of rehabilitation programs. Recently, it is reported that strengthening exercise with low-intensity resistance training (20-30% of 1 Repetition Maximum) combined with the application of blood flow restriction potentially increases muscle strength and induces muscle hypertrophy as well. Objective: This study aims to compare the increase in muscle strength and hypertrophy among the muscles trained with high- intensity resistance training (HIRT), low-intensity resistance training combined with the application of blood flow restriction using a sphygmomanometer cuff (LIRT+BFR) and low-intensity resistance training (LIRT) alone. Method: The subjects (n=18) are randomly and equally divided into three groups, those are the groups getting the strengthening exercise for left Biceps Brachii muscle with HIRT (70% of 1 RM), LIRT (30% of 1 RM)+BFR and LIRT (30% of 1 RM). Before starting and after getting the resistance training for five weeks, each subject is measured for the left arm circumference. Results: HIRT and LIRT+BFR produce an increase of the left arm circumference significantly greater than LIRT. However, there is no significant difference in the increase of left arm circumference between the HIRT and LIRT+BFR. There is a significant difference in the increase of the peak torque of left flexion elbow joint among the three groups, either at 60o/s or 120o/s or 180o/s angular velocity. At 60o/s angular velocity, LIRT+BFR produces the greatest increasing of the left elbow peak torque among the three groups. At both 120o/s and 180o/s angular velocity, there is no significant difference in the increase of the peak torque of flexion left elbow joint between HIRT and either LIRT+BFR or LIRT. However, at both 120o/s and 180o/s angular velocity, there is a significant difference in the increase of the peak torque of flexion left elbow joint between LIRT+BFR and LIRT. Conclusion: the strengthening exercise for Biceps Brachii muscle with LIRT+BFR induces the Biceps Brachii muscle hypertrophy and increases the muscle strength more than strengthening exercise with LIRT alone. Compared to the HIRT, LIRT+BFR induces an equal muscle hypertrophy and increases moremuscle strength for the trained Biceps Brachii muscle

Efek Pemberian Ekstrak Etanol 70% Barleria Lupulina Lindl dan Kombinasi Latihan Fisik Pada Mencit yang di induksi Deksametason

Barleria lupulina Lindl is one of Indonesia medicinal plants that contain iridoid, phenylpropanoid, lignin and benzyl alcohol glycoside. Traditionally it was used as antidiabetic, anti-inflammatory, antirheumatic and antiosteoporosis. Based on the previes study, the 70% ethanolic extract, buthanolic fraction and water fraction of B.lupulina stimulates alkaline phosphatase as marker of osteoblast cells (bone formation). In this research, study on the antiosteoporotic activity of 70% ethanolic extracts of B.lupulina and exercise combination on mice that induced by dexamethasone has been done. The result showed that 70% ethanolic extract of B.lupulina increase the number of osteoblast cells and rigidity of the bone matrix by morphology figure. The exercise combination showed the highest number of osteoblast cells.Key words : Barleria lupulina Lindl, osteoblast cells, bone matri

Efek penghambatan ekspresi ERβ bebas oleh fraksi n-butanol daun semanggi (Marsilea crenata Presl.) pada sel hFOB 1.19

Wanita pascamenopause seringkali mengalami berbagai macam masalah kesehatan, diantaranya adalah osteoporosis yang diakibatkan gangguan produksi hormon estrogen dalam tubuh. Salah satu alternatif yang potensial menggantikan hormon estrogen adalah fitoestrogen. Daun Semanggi (Marsilea crenata Presl.) diketahui mengandung senyawa fitoestrogen dengan kadar tinggi. Tujuan dari penelitian ini adalah untuk mengetahui efek fraksi n-butanol daun M. crenata yang mengandung fitoestrogen dalam meningkatkan aktivitas human fetal osteoblast 1.19 (hFOB 1.19) cells pada proses pembentukan tulang. Peningkatan aktivitas sel hFOB 1.19 diketahui melalui pengukuran ekspresi estrogen receptor β (ERβ) bebas dengan metode immunocytochemistry dan instrumen confocal laser scanning microscopy (CLSM). Fraksi n-butanol diberikan dengan varian konsentrasi 62,5; 125; dan 250 ppm, serta genistein sebagai kontrol positif diberikan dengan konsentrasi 1 μM. Hasil penelitian menunjukkan bahwa fraksi n-butanol daun M. crenata dapat meningkatkan aktivitas sel hFOB 1.19, yang ditunjukkan dengan penurunan ekspresi ERβ bebas pada sel hFOB 1.19 setelah pemberian fraksi n-butanol semua konsentrasi, yaitu sebesar 594.108; 1245,216; dan 1205,592 arbitrary unit (AU) pada p<0,05. Dari data dapat disimpulkan bahwa fraksi n-butanol daun M. crenata potensial dikembangkan sebagai produk antiosteoporosis

CORRELATION OF Ki67 EXPRESSION TO DEGREE OF DYSPLASIA IN LINGUAL EPITHELIAL DYSPLASIA IN Rattus norvegicus EXPOSURED BY CIGARETTE SMOKE (Hubungan Ekspresi Ki67 Terhadap Derajat Displasia Pada Epitel Mukosa Lidah Rattus norvegicus yang Dipapar Asap Rokok)

Background: Dysplasia is malignant transformation of stratified squamous epithelium characterized by cellular atypia and loss of stratification and maturation. It can caused by cigarette smoke. Measuring the activity of cell proliferation as the beginning of dysplasia is very useful to know the development of malignancy. One of proliferation marker is Ki67 that expressed in all cell cycle phase except G0.Methods: 30 male wistar rats divided into: control grup (n=10), exposed to cigarette smoke for 4 weeks (n=10), 8 weeks (n=10) as much as 20 cigarettes/group/day/exposure used smoking pump. Specimen were stained HE to see dysplasia and Ki67 expression seen immunohistochemically. Statistical analyzed by Spearman correlation.Result: There was an increase in mean of Ki67 expression and significant differences between groups (p=0,000) and also significant difference of degree of dysplasia between groups (p=0,000). There was a strong correlation between Ki67 score to degree of dysplasia (p=0.001).Conclusion: Increased of Ki67 expression proportional to the Increase in degrees of dysplasi

Investigation of altering single-nucleotide polymorphism density on the power to detect trait loci and frequency of false positive in nonparametric linkage analyses of qualitative traits

Genome-wide linkage analysis using microsatellite markers has been successful in the identification of numerous Mendelian and complex disease loci. The recent availability of high-density single-nucleotide polymorphism (SNP) maps provides a potentially more powerful option. Using the simulated and Collaborative Study on the Genetics of Alcoholism (COGA) datasets from the Genetics Analysis Workshop 14 (GAW14), we examined how altering the density of SNP marker sets impacted the overall information content, the power to detect trait loci, and the number of false positive results. For the simulated data we used SNP maps with density of 0.3 cM, 1 cM, 2 cM, and 3 cM. For the COGA data we combined the marker sets from Illumina and Affymetrix to create a map with average density of 0.25 cM and then, using a sub-sample of these markers, created maps with density of 0.3 cM, 0.6 cM, 1 cM, 2 cM, and 3 cM. For each marker set, multipoint linkage analysis using MERLIN was performed for both dominant and recessive traits derived from marker loci. Our results showed that information content increased with increased map density. For the homogeneous, completely penetrant traits we created, there was only a modest difference in ability to detect trait loci. Additionally, as map density increased there was only a slight increase in the number of false positive results when there was linkage disequilibrium (LD) between markers. The presence of LD between markers may have led to an increased number of false positive regions but no clear relationship between regions of high LD and locations of false positive linkage signals was observed